Event Abstract Back to Event Antiproliferative activity of VLC fractions obtained from Asparagopsis armata associated bacteria João F. Francisco1, Andre Horta1, Nádia S. Fino1, Susete Piteus1, Celso Alves1 and Rui Pedrosa1* 1 Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, Portugal Many studies are showing the presence of bioactive compounds in marine organisms, such as algae and algae associated bacteria. Cancer is one of the major causes of death in the world, consequently research for new antitumor compounds is continuous and have high importance for the human health. The aim of this study was to evaluate the antitumor activity of Shewanella sp. associated bacteria from Asparagopsis armata. Crude extract of associated bacteria was obtained with methanol and dichloromethane (1:1) extraction. Then the crude extract was fractioned by vacuum liquid chromatography (VLC) using cyclohexane with increasing amounts of 25% of ethyl acetate, in order to isolate different compounds obtained five fractions (F1-F5). The cell viability and the cell proliferation studies were performed on human breast adenocarcinoma cell line (MCF-7 cells) according to MTT method. In cytotoxicity assay (1mg/ml; 24 hours), the highest reduction of MCF-7 viability was induced by F2 and F3 fractions (53.6% and 48.6% respectively). On the other hand in cell proliferation assay (1mg/ml; 24 hours), all fractions showed anti-proliferative activity (1mg/mL), however the highest inhibition of MCF-7 proliferation was exhibited by F3 and F5 fractions, 15% and 17,7%, respectively. These results suggest that the Shewanella sp. associated bacteria from Asparagopsis armata can be an interesting source of new antitumor drugs. Keywords: associated bacteria, algae, Marine compounds, antitumoral therapies, Natural Products, marine symbiosis Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: BLUE BIOTECH Citation: Francisco JF, Horta A, Fino NS, Piteus S, Alves C and Pedrosa R (2014). Antiproliferative activity of VLC fractions obtained from Asparagopsis armata associated bacteria. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00064 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 May 2014; Published Online: 18 Jul 2014. * Correspondence: Dr. Rui Pedrosa, Marine Resources Research Group (GIRM), ESTM, Polytechnic Institute of Leiria, Peniche, Portugal, rpedrosa@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers João F Francisco Andre Horta Nádia S Fino Susete Piteus Celso Alves Rui Pedrosa Google João F Francisco Andre Horta Nádia S Fino Susete Piteus Celso Alves Rui Pedrosa Google Scholar João F Francisco Andre Horta Nádia S Fino Susete Piteus Celso Alves Rui Pedrosa PubMed João F Francisco Andre Horta Nádia S Fino Susete Piteus Celso Alves Rui Pedrosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.