Phytoplankton Composition Research Articles

Detection of Autumnal Concentration of Coscinodiscus granii in the Southern Baltic—A Method for In Situ Measurement of Marine Particles

Efficient means for measuring the abundance and species composition of phytoplankton in situ continue to pose a big challenge to scientists. Hitherto, analyses and interpretations have been based mainly on small numbers of data acquired from microscopic examinations of water samples. Hence, information on devices facilitating such measurements is highly desirable. This paper examines the opportunities offered by the LISST-100X instrument for measuring in situ the concentrations and spatially variable biovolumes of a species dominant in the southern Baltic during the autumnal bloom. Microscopic analysis of phytoplankton in water sampled from different locations in the southern Baltic confirmed earlier results, indicating that this bloom was due to the overriding prevalence of one microplankton diatom species—Coscinodiscus granii. Combining the microscopic measurements of C. granii cell sizes with the size distribution ranges employed by the LISST-100X yielded equivalent spherical diameters (EDSs) ranging from 47.4 to 188.0 µm, with maxima in the 78.4–92.6 and 92.6–109 ranges. Comparative analysis of the particle size distribution (PSD) spectra was used to separate the abundance of C. granii from the total suspended particulate matter (SPM). Spatial in situ measurements in 2012 and 2014 of C. granii concentrations in surface waters showed that both its abundance and its percentage contribution to the total SPM were highly variable. Over a distance of several km, these concentrations varied from values close to zero to 0.2 µL L−1 in 2012 and from 0.3 to 0.9 µL L−1 in 2014, whereas the percentage in the total SPM was found to vary locally from a few to c. 50%. The proposed method and results demonstrate the success of the LISST-100X instrument in detecting size and volume concentrations of phytoplankton in size classes ranging from 1.25 to 250 μm. However, the correct interpretation of LISST data requires that the dominant phytoplankton species concentration in the suspensions be large enough for the signal (peak) to be readily visible against the background PSD of other SPM.

Composition, Density and Diversity of Phytoplankton in Rathi Cheruvu, Bhadradri Kothagudem, Telangana, India

The current study deals with composition, density and diversity of phytoplankton during January 2023 to December 2023 monthly in the Rathi cheruvu, Bhadradri Kothagudem, Telangana, India. Samplings were collected from different locations of the study site during a one year research period. Several physico-chemical parameters were studied from two sampling stations such as water temperature, pH, DO, BOD, SO4, NO3, PO4 and Cl-, of which pH, DO and BOD were shown to be the most significant in growth and development of phytoplanton. In the current study revealed a total number of 43 species of phytoplankton belonging to four major classes i.e. Chlorophyceae with 16 species, Bacillariophyceae with 14 species, Euglenophyceae with three species and Cyanophyceae with 10 species. The maximum population of phytoplankton diversity was observed during summer followed by monsoon with a total 1058 phytoplankton species during one year of study period. Finally the study showed that the study site has a well balanced phytoplankton community.

Optical characteristics of dissolved organic matter in relation to phytoplankton in Lake Erhai, China

Dissolved organic matter (DOM), as the primary form of organic carbon in lakes, is crucial for the lake carbon cycling. As an important component of lake ecosystems, phytoplankton plays a crucial role in carbon fixation. However, we are not yet clear about the contribution of phytoplankton composition to DOM. To clarify this issue, we conducted we conducted continuous monitoring for 6 months in Lake Erhai, a plateau lake in China. Through quantitative detection of phytoplankton and spectral characteristic analysis, four fluorescence components were identified: tryptophan-like (C1), tyrosine-like (C2), fulvic-like (C3), and humic-like (C4). The Fluorescence Index (FI) ranged from 1.70 to 2.54, and the Biogenic Index (BIX) was above 0.8, indicating the DOM in Lake Erhai’s overlying water was predominantly autochthonous. Cyanophyta prevailed in September and November, while Chlorophyta dominated in other months. Phytoplankton diversity was significantly related to DOM concentration that may be related to the difference in the DOM contribution of different phytoplankton. The effect of phytoplankton biomass on C3 and C4 was prominent. Whereas, phytoplankton did not stimulate C1 and C2 significantly, indicating that protein-like may originate from terrestrial input and microbial activity. The composition of phytoplankton significantly affects the DOM composition in Lake Erhai. Therefore, the autochthonous DOM production by phytoplankton warrants further consideration in plateau lake systems.

Seasonal Variation, Diversity, and Composition of Planktons in the Ganga River

Plankton are important components of aquatic ecosystems because they contribute to primary production, which sustains fisheries and other ecosystem functions. The study was carried out over a one-year period at three Ganga River sites: Haridwar (Bhadrabad) site A1, Bijnor (Balawali) site A2, and Muzaffarnagar (Bairaj Ganga bridge) site. Bacillariphyceae made almost 65% of the phytoplankton at location A1 14% Chlorophyceae and 14% Cynophyceae. The zooplankton consisted of Protozoa (80%) and Cladocera (20%), with Ulvophyceae accounting for 7%. At location A2, the phytoplankton composition was Bacillariphyceae (79%) and Chlorophyceae (7%). ˃ Cynophyceae (7%). Ulvophyceae (7%), and zooplankton were Protozoa (80%), Rotifera (20%). At location A3, the phytoplankton mix shifted to Bacillariphyceae (70%). ˃ Chlorophyceae (18%) ˃ Cynophyceae (12%) and Protozoa (60%) Rotifera accounts for 40%. The Simpson diversity index (D) value for phytoplankton is 0.58, whereas for zooplankton, the value is 0.53. The mean plankton density at locations A1, A2, and A3 was 2059, 2959, and 3304 individuals per liter, respectively. Only dissolved oxygen in physicochemical characteristics correlated positively with plankton density; all other metrics correlated negatively.

Combined effect of warming, nutrients, and species pool size on the seasonal variability of phytoplankton composition: A modeling perspective

AbstractThe anticipated long‐term rise in temperature can have numerous effects on freshwater phytoplankton, however, there is a great deal of uncertainty about how biogeochemical processes modify community functioning under a warming climate. Previous works regarding a large shallow lake and a mesocosm study showed a positive relationship between the temporal variability of phytoplankton composition and mean temperature, which might be a sign of warming‐stimulated instability in ecosystem processes. To elucidate whether these observations are part of a more general phenomenon, we combined a numerical model describing the dependence of algal growth on temperature and nutrient concentration with a multispecies, multi‐nutrient model. We ran simulations representative of changes in phytoplankton communities with randomized initial species composition under different temperature scenarios (current annual temperature pattern increased with 1°C, 2°C, or 3°C), various levels of nitrogen and phosphorus supply and different degrees of temporal variability in those supplies. The model outputs showed that seasonal variability in composition can be enhanced by warming through the mechanistic links between nutrient availability, temperature, and species‐specific growth rates, but the rate of increase is also dependent on nutrient ratios and the size of the species pool. Moreover, temporal variability in nutrient loads also enhanced compositional variability, but the rate of increase also depended on the level of nutrient supply. The results of our study hint that the impact of climate change and eutrophication on phytoplankton dynamics can be more intricate than spatiotemporally limited field or experimental observations would suggest.

Effects of extreme rainfall events on phytoplankton community in a subtropical eutrophic lake: a mesocosm experiment

The impact of global climate change has led to an increase in extreme rainfall events, causing fluctuations in terrigenic inputs that significantly affect aquatic communities. Lake Changhu, the third-largest freshwater lake in Hubei Province, plays a crucial role as a reservoir of aquatic germplasm resources in the middle reaches of the Yangtze River. In recent years, the lake has experienced a series of extreme rainfall events. In response to recent extreme rainfall events, a 42-day mesocosm experiment was conducted to understand the effect of terrigenic inputs on various aspects of phytoplankton in Lake Changhu, such as species composition, abundance, biomass, diversity, community turnover rates, resource use efficiency, and stability. The experiment involved the application of different terrigenic treatments, including a control group (CK, using lake water), low terrigenic input (LT), medium terrigenic input (MT), and high terrigenic input (HT). The results showed a noticeable shift in phytoplankton composition from a co-dominated state of Chlorophyta and Cyanobacteria in the CK treatment to a Cyanobacteria-dominated state in the other terrigenic treatments. Furthermore, the terrigenic inputs increased phytoplankton abundance, community turnover rates, diversity, and resistance. Comparatively, the diversity index of phytoplankton increased by 82.61%, 73.83%, and 70.41% in the LT, MT, and HT treatments, respectively, in contrast to the CK treatment. However, phytoplankton abundance decreased by 6.99%, 15.55%, and 14.76% in the LT, MT, and HT treatments. Additionally, the resource use efficiency decreased by 1.94%, 5.16%, and 14.19% in the LT, MT, and HT treatments, respectively, compared to the CK treatment. These findings provide valuable insights into monitoring and managing the water ecology in Lake Changhu, offering a scientific basis for implementing effective management strategies.

Nutrient enrichment alters phytoplankton biomass and composition via silicon limitation

Despite management efforts, anthropogenic nutrient enrichments continue to enhance phytoplankton blooms worldwide. Release of nitrogen and phosphorus compounds not only provides surplus of nutrients but also disbalances their stoichiometry. Declines in the relative availability of dissolved silicon might induce limitation in diatoms, major primary producers with silicified shells. We studied experimentally how nutrient enrichment and resulting decline in dissolved silicon to nitrogen ratios (Si:N) affect the structure and functioning of natural plankton communities. Nitrate was added to create a range of Si:N ratios and phosphate was supplied in Redfield ratio to nitrogen. We also manipulated copepod abundance to understand the top-down effects on communities experiencing nutrient enrichment. Nitrogen and phosphorus additions resulted in a steep phytoplankton biomass increase, followed by a post-bloom decline. Phytoplankton bloom biomass was higher in high nitrogen treatments but during the post-bloom period this trend switched. Biomass was sustained longer in high Si:N treatments, indicating that silicon limitation terminates the bloom. Many diatom species did not benefit from nitrogen and phosphorus enrichment and diatom dominance ceased below Si:N of 0.4:1. Under high grazing pressure, silicate was taken up faster suggesting that silicification is important in diatom defense. Copepods shaped plankton communities via feeding on dinoflagellates, chlorophytes and the diatom Skeletonema costatum but there was no significant effect of nitrogen and phosphorus enrichment on copepod abundance. Our results, combined with previous studies, show that while nutrient concentrations define the total phytoplankton bloom biomass, resource ratios are important in sustaining biomass and determining community structure and composition.

Dissolved carbohydrates and its influence on bacterioplankton diversity in the euphotic zone of Equatorial Indian Ocean during southwest monsoon

The Equatorial Indian Ocean (EIO) is oligotrophic in nature, low in productivity and characterised by relatively high bacterial biomass and heterotrophic productivity. The microbial biomass is fuelled by dissolved organic carbon (DOC) whose lability depends upon its chemistry. During an expedition in the EIO, samples for dissolved carbohydrates (DCHO), dissolved monosaccharide (MCHO), dissolved polysaccharide (PCHO), total bacterial abundance, total organic carbon (TOC), nutrients, chlorophyll a (chl a), bacterial diversity, and phytoplankton composition were estimated along two transects (TS-1 & TS-2) in the euphotic depth (200 m). DCHO and MCHO samples were analysed using 3-methyl-2-benzothiozolinone hydrazone hydrochloride (MBTH) method. The DCHO, MCHO, and PCHO concentrations in TS-1 ranged from 2.4 to 5.8 μM; 0.7 to 2.7 μM; 0.7 to 3.4 μM, while in TS-2 it ranged from 2.2 to 4.8 μM; 0.3 to 2.8 μM; 0.2 to 3.8 μM, respectively. Vertically, DCHO concentrations decreased within the photic depths in most stations. PCHO concentrations were relatively greater in TS-2 than TS-1, which may be due to preferential removal of MCHO in TS-2. In the top 200 m of the water column, Proteobacteria was the most abundant bacterial phylum followed by Cyanobacteria, Actinobacteriota, Bacteroidota, and SAR-324 while dinoflagellates dominated the phytoplankton population. The distribution of DCHO and PCHO were inversely correlated to salinity, temperature, and chl a. The bacterial heterotrophs like alpha-Proteobacteria, Chloroflexii, and Verrumicrobiota along with SAR324 and Actinobacteriota were clustered with nutrients, while Bdellovibriota and Dadabacteria clustered with MCHO, PCHO, and DCHO. These results highlight the impact of DCHO & PCHO lability on the distribution of bacterial community and delineate oceanographic factors that regulate the distribution of DCHO and its constituents in EIO waters.

Phytoplankton functional dynamics in relation to some physicochemical parameters in Lake Kuriftu (Oromia, Ethiopia)

The water quality of Lake Kuriftu is deteriorating, and monitoring programs are almost nonexistent in Ethiopia. Phytoplanktons are good indicators of environmental changes due to their fast response to a wide range of stressors. The phytoplankton functional groups (FGs) can be used as water quality indicators. This study aimed to assess the dynamics of phytoplankton functional groups (FGs) in relation to some physicochemical water quality parameters measured at three sites in Lake Kuriftu. Samples for the analysis of biological and physicochemical parameters were collected monthly, from December 2020 to April 2021. The results of the physicochemical parameters revealed that Lake Kuriftu is turbid (21.37 to 59.86 NTU), has a thin euphotic depth (ZSD0.304 to 0.548 m), and is eutrophic. Ninety-seven (97) phytoplankton taxa were identified and classified into 16 Reynolds Functional Groups (RFGs), of which six (J, MP, X1, Lo, S1, and F) were represented by more than six species. FGs S1, SN, Lo, and Lm, which often form Harmful Algal Blooms, contributed together about 33.54 mg L−1, or 87.84 %, of the total biomass recorded for Lake Kuriftu, demonstrating the water quality of the lake is deteriorating. Among the FGs, the most dominant (in terms of biomass) was S1, followed by J at all sites and in all sampling months. The results of redundancy analysis (RDA) revealed that the main driving factors of phytoplankton FG biomass and composition were TP, ZSD, TN, Salinity, and ammonia. Among these, TP and salinity impacted the FGs negatively, while TN and ammonia influenced them positively. Thus, the physicochemical parameters have a significant impact on the distribution of phytoplankton FGs, suggesting that the use of FGs for water quality monitoring programs is useful. To promote wise use and conservation of the lake ecosystem, more intensive study should be carried out on the biological and physicochemical aspects of the lake, as it is part of a tourist attraction area and a source of income for local inhabitants.

Grazing strategies determine the size composition of phytoplankton in eutrophic lakes

AbstractAlthough the general impacts of zooplankton grazing on phytoplankton communities are clear, we know comparatively less about how specific grazing strategies interact with environmental conditions to shape the size structure of phytoplankton communities. Here, we present a new data‐driven, size‐based model that describes changes in the size composition of lake phytoplankton under various environmental constraints. The model includes an ecological trade‐off emerging from observed allometric relationships between (1) phytoplankton cell size and phytoplankton growth and (2) phytoplankton cell size and zooplankton grazing. In our model, phytoplankton growth is nutrient‐dependent and zooplankton grazing varies according to specific grazing strategies, namely, specialists (targeting a narrow range of the size‐feeding spectrum) vs. generalists (targeting a wide range of the size‐feeding spectrum). Our results indicate that grazing strategies shape the size composition of the phytoplankton community in nutrient‐rich conditions, whereas inorganic nutrient concentrations govern phytoplankton biomass. Under oligotrophic regimes, the phytoplankton community is dominated by small cell sizes and the grazers have little to no impact. Under eutrophic regimes, dominating specialist grazers push phytoplankton towards small cells, whereas dominating generalist grazers push phytoplankton towards large cells. Our work highlights that trait‐based modeling, based on realistic eco‐physiological trade‐offs, represents a valuable tool for disentangling the interactive roles played by nutrient regimes and grazing strategies in determining the size compositions of lake phytoplankton. Ultimately, our study offers a quantitative basis for understanding how communities of lake phytoplankton may reorganize in the future in response to changes in nutrient levels and zooplankton grazing strategies.

Environmental factors controlling seasonal and spatial variability of zooplankton in thermokarst lakes along a permafrost gradient of Western Siberia

Humic thermokarst lakes of permafrost peatlands in Western Siberia Lowland (WSL) are major environmental controllers of carbon and nutrient storage in inland waters and greenhouse gases emissions to the atmosphere in the subarctic. In contrast to sizable former research devoted to hydrochemical and hydrobiological (phytoplankton) composition, zooplankton communities of these thermokarst lakes and thaw ponds remain poorly understood, especially along the latitudinal gradient, which is a perfect predictor of permafrost zones. To fill this gap, 69 thermokarst lakes of the WSL were sampled using unprecedented spatial coverage, from continuous to sporadic permafrost zone, in order to assess zooplankton (Cladocera, Copepoda, Rotifera) diversity and abundance across three main open water physiological seasons (spring, summer and autumn). We aimed at assessing the relationship of environmental factors (water column hydrochemistry, nutrients, and phytoplankton parameters) with the abundance and diversity of zooplankton. A total of 74 zooplankton species and taxa were detected, with an average eight taxa per lake/pond. Species richness increased towards the north and reached the maximum in the continuous permafrost zone with 13 species found in this zone only. In contrast, the number of species per waterbody decreased towards the north, which was mainly associated with a decrease in the number of cladocerans. Abundance and diversity of specific zooplankton groups strongly varied across the seasons and permafrost zones. Among the main environmental controllers, Redundancy Analysis revealed that water temperature, lake area, depth, pH, Dissolved Inorganic and Organic Carbon and CO2 concentrations were closely related to zooplankton abundance. Cladocerans were positively related to water temperature during all seasons. Copepods were positively related to depth and lake water pH in all seasons. Rotifers were related to different factors in each season, but were most strongly associated with DOC, depth, CH4, phytoplankton and cladoceran abundance. Under climate warming scenario, considering water temperature increase and permafrost boundary shift northward, one can expect an increase in the diversity and abundance of cladocerans towards the north which can lead to partial disappearance of copepods, especially rare calanoid species.

Spatial and Temporal Variability in Water Quality and Phytoplankton Community Composition in Charleston Harbor

The Charleston Harbor estuary is a dynamic ecosystem draining three rivers that surround the rapidly urbanizing greater Charleston area. Projected climate change impacts include elevated sea surface temperature and local changes in water quality that will likely alter biogeochemical cycling as well as phytoplankton abundance and community composition. Partnering with the local non-profit organization Charleston Waterkeeper, surface water samples were collected from late April through October 2021 at 20 sites in the Charleston Harbor estuary system. Water quality parameters measured included sea surface temperature (SST), salinity, pH, dissolved inorganic carbon, chromophoric dissolved organic matter (CDOM), phytoplankton pigments and nitrate and phosphate concentrations. Relatively high (6—16 μM) nitrate values were observed throughout the year and the nitrate to phosphate (N:P) ratios were consistently elevated (50—200) relative to the Redfield ratio of 16. Analysis of variance indicated that the delivery of nitrate, phosphate, and CDOM into the estuary came from upriver of the Charleston Harbor. For instance, CDOM was significantly impacted by tidal stage (p < 0.05) and was negatively correlated with salinity at low salinity sites, but no correlation was observed at high salinity sites. Seasonal patterns in phytoplankton community abundance and composition were driven by changes in SST, as overall phytoplankton biomass increased with a community shift from diatoms during colder months to flagellated cells such as prasinophytes during the warmer summer months. Phytoplankton diversity was greatest during the early summer and lowest in October. This study provides a reference baseline for water quality parameters and phytoplankton community composition in an estuarine ecosystem that is changing rapidly due to dredging and climate change processes.

Biomonitoring of the surface waters of the Volyntsevo reservoir using the fluorimetry method

The article examines the state of the drinking Volyntsevo reservoir and its tributaries in conditions of significant changes in the hydraulic regime and pollution by waste mine waters. The state of algoflora of a water body is considered, the results of studying the species composition of phytoplankton, the content of basic photopigments and photosynthetic activity of microalgae are presented. The study presents the results of the application of the method of fluorimetric assessment of the state of phytoplankton cells as the main bioindicator of the state of the aquatic environment. The research was conducted in the vicinity of Yenakievo for the Volyntsevo reservoir and the Bulavin river. These water bodies are subject to significant man-made impacts: as a result of intensive water use of reservoir resources, there has been a significant decrease in water levels and, as a result, a deterioration in water quality. The conducted studies have shown a significant excess of the permissible standards for the content of magnesium ions, sulfates, total hardness and alkalinity in water samples. The negative impact of surface water pollution on the species composition of algoflora, as well as the photosynthetic activity of phytoplankton cells, has been shown. The obtained materials can serve as a basis for further monitoring of drinking reservoirs in the region. The current situation requires constant monitoring observations aimed at identifying and eliminating all sources of pollution of the studied water bodies.

Past environmental changes: using sedimentary photosynthetic pigments to enhance subtropical reservoir management.

The historical impacts of eutrophication processes were investigated in six subtropical reservoirs (São Paulo, Brazil) using a paleolimnological approach. We questioned whether the levels of pigment indicators of algal biomass could provide information about trophic increase and whether carotenoid pigments could offer additional insights. The following proxies were employed: organic matter, total phosphorus, total nitrogen, photosynthetic pigments (by high-performance liquid chromatography), sedimentation rates, and geochronology (by 210 Pb technique). Principal component analysis indicated a gradient of eutrophication. In eutrophic reservoirs (e.g., Rio Grande and Salto Grande), levels of lutein and zeaxanthin increased over time, suggesting growth of Chlorophyta and Cyanobacteria. These pigments were significantly associated with algal biomass, reflecting their participation in phytoplankton composition. In mesotrophic reservoirs, Broa and Itupararanga, increases and significative linear correlations (r > 0.70) between pigments and nutrients are mainly linked to agricultural and urban activities. In the oligotrophic reservoir Igaratá, lower pigment and nutrient levels reflected lesser human impact and good water quality. This study underscores eutrophication's complexity across subtropical reservoirs. Photosynthetic pigments associated with specific algal groups were informative, especially when correlated with nutrient data. The trophic increase, notably in the 1990s, may have been influenced by neoliberal policies. Integrated pigment and geochemical analysis offers a more precise understanding of eutrophication changes and their ties to human factors. Such research can aid environmental monitoring and sustainable policy development.

Plankton Diversity and Hydro-biological Parameters at Wazirabad Barrage in Yamuna River

This study conducted a comprehensive investigation into the qualitative and quantitative analysis of plankton diversity and Physico-chemical water quality parameters in the Yamuna River. Samples were collected from the Wazirabad barrage from June 2022 to November 2022, revealing a diverse phytoplankton community consisting of 50 genera distributed across five major classes. The abundant classes were Bacillariophyceae, with 18 genera, followed by Chlorophyceae with 20 genera, Cyanophyceae with 9 genera, Euglenophyceae with 2 genera, and Dianophyceae represented by a single genus. The qualitative analysis uncovered a zooplankton community with 20 genera from Protozoa and Rotifera, Copepoda, and Cladocera. Protozoa were represented by two genera, Copepods exhibited eight genera, Cladocera included five genera, and Rotifera featured four genera. The most abundant class of phytoplankton is Chlorophyceae and Zooplankton is Copepods. Physico chemical water quality parameters more fluctuation during the study period. These findings offer valuable insights into the biodiversity and composition of phytoplankton and zooplankton communities in the Wazirabad Barrage ecosystem.

Implications of Anthropic Activities in the Catchment Area of a Temporary Mediterranean Wetland Complex in the South of Spain

The Lagunas de Campillos Natural Reserve and adjacent ponds are fundamentally surrounded by regularly fertilized crop fields and livestock industry, producing leachates which can be found in the ponds. The interest in this Site of European Importance and the RAMSAR wetland complex lies in the habitats within it, which are included in the Directive on Habitats of Community Interest. It is essential to determine the trophic status of the ponds and the quality of these habitats, as well as whether corrective measures need to be established in order to maintain a good environmental status. To characterize and compare the ponds, different parameters were measured, such as conductivity, pH, nutrient concentration, Chl-a concentration, phytoplankton composition, phytoplankton abundance (<20 µm), and the quantification of heterotrophic microorganisms indicating contamination of the aquifers. The obtained results showed that all ponds, except a mesotrophic pond, are eutrophic or even hypertrophic, with high levels of total nitrogen (>8 mg L−1), total phosphorous (>165 μg L−1), and chlorophyll-a concentration. These findings explain the high densities of phytoplankton observed, with the predominant presence of small cells (<3.6 μm ESD). In addition, concentrations of heterotrophs and coliforms are, in some ponds, higher than expected. Eutrophication hinders ecological functions and ecosystem services, which finally affects biodiversity and human wellbeing. Five of the six analyzed ponds are within various protection figures for their essential importance to local and migrating avifauna. Therefore, ponds’ status analysis and the implementation of measures for maintaining ecosystem services and trophic state are fundamental for the sustainable management of the studied area.

Environmental driving forces and phytoplankton diversity across the Ross Sea region during a summer–autumn transition

AbstractThe Ross Sea is a highly productive system characterized by a seasonal succession of phytoplankton groups. However, most of the current understanding is based on observations on the continental shelf in spring and summer at relatively coarse taxonomic resolution. Here, we characterize community composition (class to species) using V4‐18S rRNA gene metabarcoding on transects to and across the Ross Sea (shelf‐slope and oceanic subregions) during two voyages covering the Austral summer–autumn progression in successive years. Phytoplankton composition shifted from low‐diversity diatom‐dominated (50%) communities during the summer to more diverse dinoflagellate‐dominated (48%) systems during the lower‐productivity autumn season. Prymnesiophyceae abundance was low on both voyages (10%), except on the southeast shelf‐slope, where Phaeocystis antarctica dominated a shallow mixed layer, contrary to its reported preference for deep‐mixing conditions. Amplicon sequence variant analysis identified distinct spatial patterns for two P. antarctica genotypes yet clustered certain species of Bacillariophyta and Prymnesiophyceae, indicating similar environmental preference for genotypes in these groups. Multivariate analysis of environmental drivers found a higher correlation of community composition variation with salinity and macronutrients, but less so with mixed layer depth, considered a primary determinant of taxonomic composition in the Ross Sea. Comparison between years established that community composition was temporally more stable in oceanic relative to shelf‐slope waters. This study of seasonal variation of phytoplankton community composition at finer taxonomic resolution provided insights into species‐ and strain‐specific distribution, ecological preferences, and relationships to environmental conditions in the wider Ross Sea to inform modeling and projection of future regional changes.

Metabarcoding and microscopy characterization of phytoplankton from frontal areas of the Argentine Sea

The Argentine Sea is worldwide recognized as a highly productive area, characterized by extensive phytoplankton blooms during spring and summer. Despite the well-known importance of frontal areas for biomass accumulation, phytoplankton diversity remains poorly studied. In an unprecedented approach for the Argentine Sea, we combined microscopy and 18Sv4 metabarcoding analyses for a refined assessment of summer phytoplankton composition in three understudied frontal areas of the Argentine Sea (≈43°−55°S), with contrasting oceanographic conditions. Metabarcoding and microscopy analyses agreed on the detection of the dominant phytoplanktonic groups in the different frontal areas studied; chlorophytes in Valdés Peninsula, dinoflagellates in waters off Blanco Cape, and diatoms in de los Estados Island. The analysis of the phytoplankton community was significantly enriched by combining both techniques, microscopy provided cell abundances and biomass data and metabarcoding provided greater detail on species composition, revealing an important specific richness of dinoflagellates, diatoms and other delicate groups, such as chlorophytes. However, we also considered differences between the methods for certain taxa at a lower taxonomic level (species/genus) of the dominant taxa, such as the underestimation of the diatoms Asterionellopsis glacialis and Pseudo-nitzschia spp. and the overestimation of Chaetoceros contortus by metabarcoding in comparison to microscopic counts. The detection of several taxa belonging to small and delicate groups, previously overlooked due to the lack of distinct morphological features, establishes a baseline for future studies on phytoplankton diversity in the Argentine Sea.

Tannic Acid Content in the Cultured Diatom Skeletonema costatum (Greville) Cleve 1873, Isolated from the Romanian Black Sea Waters

Skeletonema costatum is a dominant species in the spring phytoplankton composition of the Romanian Black Sea coastal waters. The species was isolated during a spring bloom and maintained in laboratory conditions for the past 10 years. The biomass was obtained by culturing the species in growth medium for diatoms under continuous light and shaking for four days. The biomass was harvested by vacuum filtration and lyophilized. The tannic acid content was determined by the HPLC method in the methanolic etract of S. costatum biomass. The tannic acid content was 0.46% (4.6 mg tannic acid/g biomass). Also, the chromatogram showed the presence of other separate, but unidentified organic compounds, which may contribute to its therapeutic potential. Our results justify further research to highlight S. costatum antioidant and antimicrobial activity.

Influence of hydrological changes on the composition of phytoplankton and sediment diatoms in the Rio de la Plata estuary

ABSTRACT ENSO phases produce changes in precipitation and river flow in Eastern South America. We aimed to evaluate phytoplankton and surface sediment diatoms' response to ENSO within the Río de la Plata estuary. A 180-km-long transect off the Uruguayan coast was undertaken in May 2009 (La Niña) and May 2010 (El Niño), where physical, chemical and biological variables were measured. During El Niño we registered lower temperatures/salinities throughout the transect, and the riverine zone was specifically more homogeneous in terms of temperature, salinity, turbidity and chlorophyll-a than during La Niña. We observed changes in the species composition of both planktonic and sediment diatoms. Concomitantly, a significant depletion in planktonic diatom abundance and richness in both planktonic and benthic domain during El Niño was observed. In addition, there was a higher proportion of dinoflagellates than during La Niña, which was explained by a higher water column stability. The dominance of the chain-forming and meso-eutrophic diatom Aulacoseira was registered during El Niño in both domains, mainly in the fluvial zone, related to a high river discharge. Our findings are important to anticipate changes in microalgae composition related to oceanographic variability during potential ENSO events.