Recent studies of late Quaternary landscape evolution in stream valleys throughout the central Great Plains of North America have identified thick packages of MIS 3-age alluvium and colluvium beneath terraces, alluvial fans, and footslopes. Here, we review and build on these studies to highlight the geomorphic history of river systems in the Central Plains during MIS 3 and reconstruct the paleoclimatic conditions and vegetation communities that existed in the region at that time.A suite of 50 radiocarbon and optically stimulated luminescence ages indicate that late MIS 3 was a period of widespread landscape stability and soil development in the Central Plains. In general, river systems were actively aggrading during early MIS 3, but aggradation slowed after ca. 40 ka, allowing soils to develop until ca. 30–27 ka. In some river valleys, a second pulse of aggradation occurred shortly after that time followed by a period of landscape stability and pedogenesis during early MIS 2 (ca. 24–19 ka). Evidence for widespread landscape stability in stream valleys implies that high magnitude flooding was minimal. Atmospheric circulation patterns that favor these conditions include zonal airflow that inhibits incursions of moist, maritime air from the Gulf of Mexico.Paleoenvironmental conditions during late MIS 3 were reconstructed using the stable carbon, oxygen, and hydrogen isotopic composition of pedogenic phyllosilicates, carbonates, and organic matter. Estimated paleotemperatures ranged from 6 to 9 °C ± 3 °C and are approximately 4–7 °C lower than modern mean annual temperature. The estimated δ18O values of soil water from phyllosilicates range from −10.6‰ to −9.6‰. These estimates are lower (18O-depleted) by about 1.5–3‰ relative to modern meteoric δ18O values and we estimate that the δ18O of paleoprecipitation was likely 18O-depleted by up to 8.5‰ relative to modern values. Our estimated δ18O values for soil waters in the Central Plains during late MIS 3 can be explained by climatic conditions characterized by 1) cooler temperatures, 2) precipitation being sourced primarily from Pacific/Arctic air masses, 3) seasonal changes in precipitation patterns (i.e., predominantly winter precipitation), or 4) a combination of the above. δ13C values indicate the presence of a notable proportion of C4 vegetation in the Central Plains during late MIS 3. We suggest that C4 productivity was likely favored at that time by a low atmospheric CO2 environment that had sufficiently warm temperatures and effective precipitation during the growing season.