ObjectiveTo assess literature for the effectiveness and impact of femtosecond (fs) laser irradiation for surface processing of enamel and dentine. Materials and methodsThis systematic review followed PRISMA guidelines. Criteria for inclusion and exclusion were established, and searches undertaken in the PubMed/MEDLINE, Embase and, Scopus databases for articles published in English over a period of the last 25 years. In-vivo studies were excluded. Analysis explored the effects of varying laser parameters including the ablation threshold, ablation rate, relative ablation efficiency and the influence of laser processing parameters, such as the laser scanning speed, on purported surface changes and presumed thermal effects on dental enamel and dentin that arise directly as a result of fs laser irradiation. ResultsEleven articles that met the inclusion criteria reported that fs laser pulses can progressively remove (i.e., ablate) dental enamel and dentin with an acceptable precision and accuracy, with little or no observed thermal effects. These reported the use of an ultra-fast fs lasers employing different wavelengths, pulse durations and irradiation parameters, such as the output power and the pulse repetition rates. The chemical composition of enamel and dentin was found not to be significantly altered by an fs laser treatment. ConclusionSignificant efforts are still required to optimise fs laser parameters for precise and selective ablation of dental tissue and dentine to enable minimally invasive restorative procedures.