To achieve efficient utilization of waste sand and make it a recyclable resource, the waste sand was reinforced by microbially induced calcium carbonate precipitation (MICP). Scanning Electron Microscopy (SEM)–Energy Dispersive Spectrometer (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were performed to determine the mineral morphologies and elemental compositions. The results of SEM showed that rhombohedral and dumbbell-shaped minerals filled the pores of the sand column, and the elemental compositions were C, O, Ca, Al, and P. Various organic functional groups were discovered by FTIR. Mineral compositions were analyzed by X-ray diffraction (XRD). The results showed that the mineral components were calcite and aragonite, and the crystallinity of calcite improved with the increase in the bacterial concentrations. Stable carbon isotope analyses showed that the sand columns at different bacterial concentrations ranged from −18.9‰ to −21.4‰, which were more negative than chemical calcite with −10.9‰. The mechanical properties of compression strength and splitting tensile strength proved that MICP could enhance the strength of sand columns. Thermodynamic characteristics were carefully investigated using thermogravimetric analysis from 50 °C to 1000 °C, which showed that the activation energy and thermal stability of the sand columns reinforced by MICP increased. Therefore, this study provides important insights into the process of MICP, which has good spontaneity, ecological performance, and low energy consumption. It is conducive to the construction of ecological civilization and the requirements of green development, and it has important engineering significance.