A novel mesoporous ZrO2-TiO2 (ZT) based multi-component composite oxide catalyst V2O5-WO3-CuO-Al2O3/ZrO2-TiO2 (VWCuAl/ZT) was successfully synthesized by one-pot hydrothermal method using both Brij-35 and P123 as composite template. The results indicated that the active species of V2O5, WO3, CuO and Al2O3 could be homogeneously dispersed into mesoporous ZrO2-TiO2 catalyst carrier. Compared with the reference samples of non-mesoporous I-VWCuAl/ZT and the traditional commercial catalyst V2O5-WO3/TiO2(V-W/Ti), the prepared VWCuAl/ZT showed a superior low-temperature catalytic activity and high stability over the reaction of NH3-SCR of NO, especially high catalytic efficacy for NO reduction within a wide temperature range of between 200 degrees C to 500 degrees C.