ABSTRACT A new long-span assembly composite floor system for use in multi-column frame-tube structure is presented. The basic assembly unit consists of steel truss keel and profile sheet concrete composite slab, which of the two parts are combined together through shear stud. By means of standardized assembly connection structure, such a new floor system can realize a rapid assembly of floor and support frame. Based on test research of 1/3 scale model, the paper discusses the in-plane mechanical performance of the new floor system. Through loading at the direction of parallel to the assembly slab seam and perpendicular to the assembly slab seam, respectively, the influence of assembly orientation of floor units on the failure modes, distribution and transmission performance of the horizontal loads, and the in-plane stiffness were identified in detail. A numerical model was developed with its input parameters calibrated from full-scale experimental tests. Based on model analysis, the influence of thickness of concrete slab on the in-plane stiffness was discussed.