Molecular Weight Components Research Articles

Characterisation of LC-MS-based low molecular weight compounds and fatty acids of four wild edible mushrooms

The total fatty acids and low molecular weight components were determined from four wild edible mushrooms (Tuber aetivum, Cantharellus cibarius, Boletus edulis, and Tricholoma matsutake). The fatty acids were investigated by gas chromatography-mass spectrometry (GC-MS), and the low molecular weight compounds were identified by liquid chromatograph-mass spectrometry (LC-MS) combined with multivariate statistical analysis. A total of 32 fatty acids were detected. The total fatty acids content was the highest in Tub. aetivum. Linoleic acid was the highest in the four wild edible mushrooms. Besides, a total of 144 and 91 differential with 40 mutual low molecular weight compounds were identified in positive and negative ion modes, respectively, mainly including amino acids, organic acids, sugars, and vitamins. Comprehensive multivariate statistics showed that betaine, choline, and 13 other compounds were differential low molecular weight compounds of the four wild edible mushrooms. These results expand our knowledge of the nutrition and flavour precursors in wild edible mushrooms.

Pyrolysis kinetics and product distribution of α-cellulose: Effect of potassium and calcium impregnation

Cellulose accounts for the largest proportion of lignocellulosic biomass. Herein, experimental and simulation studies are used to deeply understand the kinetic characteristics of the thermal decomposition of α-cellulose. The simulated data is in good agreement with the experimental data in the aspects of the conversion and the conversion rate versus temperature. The decomposition of α-cellulose, mainly occurring at 270–420 °C, induced an apparent activation energy ranging from 175.42 kJ/mol to 197.73 kJ/mol at a conversion of 10–90%. With 0.1–0.2 wt% K or Ca impregnation into the α-cellulose, the mean activation energy for pyrolysis was lowered (from 181.47 kJ/mol (for α-cellulose) to 141.11 kJ/mol (for 0.2 wt% K/α-cellulose) and 159.46 kJ/mol (for 0.1 wt% Ca/α-cellulose)) and higher amounts of liquid and gas products were produced. Furthermore, the addition of potassium and calcium increased the production of lower molecular weight components, such as furfural and its derivatives. The kinetic parameters of the α-cellulose pyrolysis were determined based on a nonlinear least-squares regression of the experimental data assuming first-order kinetics and correlated with the simulated result. The kinetic rate constants indicate that the predominant reaction pathway is from α-cellulose into a liquid product, rather than from α-cellulose into a gas product.

Use of an ultraviolet light-activated persulfate process to degrade humic substances: effects of wavelength and persulfate dose.

Natural organic matter (NOM), commonly found in surface and ground waters, form disinfection by-products in drinking water. Generally, advanced oxidation processes (AOPs) featuring hydrogen peroxide are used to treat water; however, sulfate radical recently has been used to treat recalcitrant organics, because it is associated with a higher oxidation potential and more effective removal than hydroxyl radicals. Hence, in this research, we evaluated persulfate oxidation efficiency in terms of reductions in humic substance levels and investigated the degradation mechanism. The results showed that ultraviolet-activated persulfate effectively treated humic substances compared with hydrogen peroxide and direct irradiation. Treatment was dose and wavelength dependent; higher persulfate concentrations or shorter UV wavelengths were more effective for treating humic substances as high concentration sulfate radicals were created. The degradation mechanism was similar to that of hydrogen peroxide. Aromatic and chromophore components were more susceptible to degradation than were lower molecular weight components, being initially decomposed into the latter, reducing UV254 absorbance and the SUVA254. Lower molecular weight materials were eventually degraded to end products: NPOC levels fell. And we also treated the inflow of a drinking water treatment plant with persulfate, and humic substances were effectively removed.

The Toxic Effects of Low Molecular Weight Components of Cow Colostrums: The Short-Term and Long-Term Effects

Background: Low-molecular components of colostrum (LMWCC) have a pronounced biological activity. We investigated the effect of different doses of LMWCC (0.01, 0.1, 1 and 5 g/100 g of body weight) on the behavior, dynamics of growth, acute and chronic toxicity, as well as the relative weight of the liver, spleen and kidneys of the experimental animals. Methods: We used 100 3-month male rats in the experiment. All animals were divided into 5 groups of 20 animals in each group, LMWCC was administered per os, and the control group received sterile water. Results: LMWCC revealed no pirogenic effect in a wide diapason of doses (0,01-5 g/100 g of animal mass). Immediately after the LMWCC administration the short-time decrease in the motion activity was observed. The low doses of LMWCC induced diarrhea in 10-20 % of of animals, the super-large dose – in 75% of animals. The acute toxicity of LMWCC (death of 15 % of animals) was detected only in super-large dose of LMWCC and it was accompanied by pronounced diarrhea. LMWCC influenced the mass of liver, spleen and kidney. 60 days after LMWCC administration the mass of liver was restored to control weight, but the mass of spleen and kidney was not restored. Conclusion: LMWCCdo not have chronic toxicity and can be attributed to non-toxic compounds with a possible side effect - a violation of the digestive system.

NiO based catalysts obtained “in-situ” for heavy crude oil upgrading: effect of NiO precursor on the catalytic cracking products composition

Heavy and super-heavy oils are characterized by a high content of resins and asphaltenes, which complicate the transportation and refining of oil and lead to an increase in the cost of refinery products. Currently, of particular interest are catalysts that are formed directly under the conditions of the process from precursors, which are various salts. In this work influence of NiO precursor on the heavy oil catalytic cracking products composition. The upgrading in all experiments was carried out at a temperature 450 °C for 80 min. The optimal catalyst content calculated to the NiO in experiments 2 and 3 was 0.5 wt. % of the feedstock weight. The ratio salt:ethanol in experiment 3 was 1:1 wt. The products of catalytic cracking were analyzed by SARA, GC, XRD and FT-IR spectrometry. The dissolution of Ni(NO3)2•6H2O in ethanol results in a decrease in the content of high molecular weight components and an increase in the yield of the fraction boiling up to 360 °C. A decrease of sulfur content in the course of heavy oil cracking is caused by both interactions of NiO formed over cracking with S-containing compounds of feedstock leading to formation of Ni9S8 and Ni0.96S and sulfur removal in form of gas.

Effects of simulated digestion in vitro on the structure and macrophages activation of fucoidan from Sargassum fusiforme

Molecular size and spatial structure affect the bioactivities of polysaccharides. SFF is a fucoidan extracted from Sargassum fusiforme. The possible changes of SFF affected by gastrointestinal tract and subsequently changes of its physicochemical property or its bioactivity have yet to be systematically investigated. Our results showed that DSFF, the gastrointestinal digestion product of SFF, has increased reducing sugar content, increased proportion of low molecular weight components, and a more clustered island-like morphology. Both SFF and DSFF activate RAW 264.7 macrophages evidenced by the increasing level of NO, intracellular ROS, and macrophages cytokines. Further investigation showed that DSFF induced M1 phenotype polarization in RAW 264.7 cells. DSFF also showed stronger macrophage activation and phenotype polarization than SFF. Our present work showed that SFF could be digested by simulated gastrointestinal environment in vitro and the digested product DSFF showed higher efficiency in macrophages activation and phenotype polarization.

Effect of flotation fractions of long-flame coal on regulation of sulfur and coke reactivity during pyrolysis of high-sulfur coking coal

The flotation fractions of a long-flame coal were obtained by heavy medium separation method, and its effect on regulation of sulfur and coke reactivity during pyrolysis of high-sulfur coking coal were investigated by FT-IR, Raman, TG, Gieseler fluidity, N2 adsorption, XRD. The results show that the low density fractions contain more aliphatic side chains and unstable aliphatic structure, while the high-density fractions show higher amount of minerals and inert components. Low density fractions have the highest sulfur removal rate due to lower content of alkaline minerals and more aliphatic side chains. The medium molecular weight component in the low density fractions has little effect on properties of metaplast. Higher minerals and inert components in high-density fractions deteriorate the metaplast more obviously. Meanwhile, order of the coke's microcrystalline structure is reduced and the defect sites increase, and consequently, reactivity of the coke increases.

Investigation of the optical, mechanical, and radiation shielding features for strontium-borotellurite glass system: Fabrication, characterization, and EPICS2017 computations

The optical, mechanical and radiation shielding features of novel (60-x) TeO2-10SrO-(30+x)B2O3 glass systems have been investigated. The density of the glasses decreases from 5.107 to 3.593 g/cm3 whereas the molar volume increases from 24.86 to 30.32 cm3 as the concentration of the TeO2 decreases. The band gap energy increases from 3.388 to 3.417 eV and from 3.083 to 3.118 eV for direct and indirect bandgap, respectively with the decreasing concentration of the TeO2. Further, the Urbach energy descends from 0.03959 to 0.03306 with an ascending insertion of the TeO2. The increase in elastic moduli indicated the improvement in the elastic properties of the samples with increase in TeO2 concentration. The mass attenuation coefficient (μ/ρ) was calculated via EPICS2017, and the results revealed that the μ/ρEPICS2017 is slightly lower than μ/ρ XCOM by an average of 0.001 cm2 g−1 for the five glasses, and this means that the two theoretical models of the five glass samples are in good agreement. The linear attenuation coefficient (µ) was calculated and we found that when the concentration of the high molecular weight component (TeO2) decreases from 60 to 40 mol%, a reduction in the µ is occurred, and the µ follows the trend: TBS30 > TBS35 > TBS40 > TBS45 > TBS50. We also determined the average half value layer (HVL̅) for each glass sample and we found that the HVL̅ varied between 1.835 cm (for TBS30) and 2.614 cm (for TBS50). An inverse relation between the TeO2 content and the HVL̅ is reported.

Influences of the hydrophilic components of two anthropogenic dissolved organic nitrogen groups on phytoplankton growth in Jiaozhou Bay, China

Increasing human activities have caused the accumulation of dissolved organic nitrogen (DON) in the ocean, which can alter dominant coastal phytoplankton species. However, insights into DON's effects on marine phytoplankton growth are insufficient compared with those of dissolved inorganic nitrogen (DIN), especially regarding the role of specific DON components. Therefore, in this study, the effects of the hydrophilic (Hic) and low molecular weight (LMW) components of two anthropogenic DON sources on the growth and bioavailable nitrogen uptake of phytoplankton were studied using in situ cultural experiments conducted in Jiaozhou Bay, China. Animal-derived DON from domestic and livestock breeding showed a higher bioavailability compared with that of vegetal DON derived from agricultural sources, with bioavailable component proportions of 76% ± 4% and 66% ± 3%, respectively. Both forms of DON could be absorbed by Skeletonema costatum, stimulating it to become the dominant species in the mesocosm ecosystem; the hydrophilic components of DON contributed approximately 75% of the uptake of DON by S. costatum. The bioavailability of LMW DON was significantly (p < 0.05) lower than that of the Hic DON. The high bioavailability of the Hic DON was mainly associated with its protein-like T1 and T2 components, identified using parallel factor analysis on the excitation-emission-matrix spectra, while the low bioavailability of LMW DON was mainly associated with the humus-like A component. The protein-like T2 components may be directly absorbed by algae, while T1 may be transformed through mineralization and algal absorption. Understanding the impacts of anthropogenic DON and its components on phytoplankton will help improve coastal environmental management. More knowledge of the effect of anthropogenic DON on the phytoplankton community structure in coastal waters should be accumulated in the future.

The Influence of Ethyl Branch on Formation of Shish-Kebab Crystals in Bimodal Polyethylene under Shear at Low Temperature

Formation of shish-kebab crystals using a bimodal polyethylene system containing high molecular weight (HMW) component with different ethyl branch contents was investigated. In situ small-angle X-ray scattering (SAXS) and wide-angle X-ray diffraction (WAXD) techniques were used to monitor the formation and evolution of shish-kebab structure sheared at low temperature in simple shear mode and low rate. Only the bimodal PE with no branch formed shish-kebab crystals at the shear temperature of 129 °C, and the shish length increased with the crystallization time, while bimodal PE with branch has no observable shish under the same conditions. The degree of crystallization for bimodal PE with no branch increased with time up to above 7%, while those with ethyl branch increased continually up to above 23%. Furthermore, bimodal PE’s Hermans orientation factor with no branch increased to 0.60, while those with ethyl branch only increased to a value below 0.15. This study indicated that the shish-kebab crystal formed at the low temperature of 129 °C is due to the stretch of entangled chains under shear for the bimodal PE with no branch. Only partly oriented lamellar crystals were formed for the bimodal PE with ethyl branch. All the results at the shear temperatures higher, closed to, and lower than the melting point, the modulation of shish crystals formation owing to different mechanisms of the coil-stretch transition and the stretched network by changing shear temperature was achieved in the bimodal PE samples.

Serum creatinine and cystatin C-based estimates of glomerular filtration rate are misleading in acute heart failure.

AimsWe aimed to test whether the endogenous filtration markers serum creatinine or cystatin C and equation‐based estimates of glomerular filtration rate (GFR) based on these markers appropriately reflect changes of measured GFR in patients with acute heart failure.MethodsIn this prospective cohort study of 50 hospitalized acute heart failure patients undergoing decongestive therapy, we applied an intravenous visible fluorescent injectate (VFI), consisting of a low molecular weight component to measure GFR and a high molecular weight component to correct for measured plasma volume. Thirty‐eight patients had two sequential GFR measurements 48 h apart. The co‐primary endpoints of the study were safety of VFI and plasma stability of the high molecular weight component. A key secondary endpoint was to compare changes in measured GFR (mGFR) to changes of serum creatinine, cystatin C and estimated GFR.ResultsVFI‐based GFR measurements were safe and consistent with plasma stability of the high molecular weight component and glomerular filtration of the low molecular weight component. Filtration marker‐based point estimates of GFR, when compared with mGFR, provided only moderate correlation (Pearson's r, range 0.80–0.88, depending on equation used), precision (r 2, range 0.65–0.78) and accuracy (56%–74% of estimates scored within 30% of mGFR). Correlations of 48‐h changes GFR estimates and changes of mGFR were significant (P < 0.05) but weak (Pearson's r, range 0.35–0.39). Observed decreases of eGFR by more than 15% had a low sensitivity (range 38%–46%, depending on equation used) in detecting true worsening mGFR, defined by a >15% decrease in mGFR.ConclusionsIn patients hospitalized for acute heart failure, serum creatinine‐ and cystatin C‐based predictions performed poorly in detecting actual changes of GFR. These data challenge current clinical strategies to evaluate dynamics of kidney function in acute heart failure.

Amino Acid Composition and Functional Properties of Different Molecular Weight Segments of Macadamia Peptides

In this study, macadamia peptides were produced by ultrasonic-assisted alkaline protease, and five components were separated by ultrafiltration centrifuge with different molecular weight (1kDa, 3kDa, 10kDa, 30kDa). The amino acid composition, solubility, water absorption capacity and emulsifying properties of five components were compared. Results showed that all macadamia peptide components were rich in amino acid species, the contents of essential and hydrophobic amino acids ranged from 189.59 to 281.10mg/g and 204.26 to 258.78mg/g, respectively. Meanwhile, the small molecular weight components (MP-1) showed the best solubility and water absorption capacity, large molecular weight components (MP-5) had the best emulsifying properties. This result indicated that the distribution of molecular weight had significant effects on the amino acid composition and functional properties of macadamia peptide components. This study provides a theoretical basis for the further development of macadamia peptide products.

Simultaneous separation of egg white proteins using aqueous three-phase partitioning systems

Egg white proteins (ovalbumin, ovotransferrin, lysozyme, ovoinhibitor and ovomucin) are used in a wide range of applications, thus requiring the development of cost-effective purification platforms for their isolation and recovery. In this work, aqueous biphasic systems (ABS) and three phase partitioning (TPP) were investigated as combined approaches to separate egg white proteins. ABS composed of poly(ethylene)glycol of different molecular weights and potassium phosphate buffer at pH 7 were first characterized and optimized to simultaneously separate ovalbumin and lysozyme. Within the ABS concept, there is the preferential partition of both proteins to the PEG-rich phase. However, by increasing the PEG molecular weight or phase-forming components content, there is the selective precipitation of lysozyme over ovalbumin at the ABS interface, falling within the TPP concept. The best TPP systems were then applied to separate proteins directly from egg white. With the best TPP identified, there is the fractionation of 3 main proteins from egg yolk, namely ovalbumin in the PEG-rich phase, lysozyme at the ABS interface and ovoinhibitor in the salt-rich phase. The obtained results reinforce the potential of ABS/TTP combined strategies to fractionate high-value proteins from complex matrices in a single-step.

Co-Amorphous Drug Formulations in Numbers: Recent Advances in Co-Amorphous Drug Formulations with Focus on Co-Formability, Molar Ratio, Preparation Methods, Physical Stability, In Vitro and In Vivo Performance, and New Formulation Strategies.

Co-amorphous drug delivery systems (CAMS) are characterized by the combination of two or more (initially crystalline) low molecular weight components that form a homogeneous single-phase amorphous system. Over the past decades, CAMS have been widely investigated as a promising approach to address the challenge of low water solubility of many active pharmaceutical ingredients. Most of the studies on CAMS were performed on a case-by-case basis, and only a few systematic studies are available. A quantitative analysis of the literature on CAMS under certain aspects highlights not only which aspects have been of great interest, but also which future developments are necessary to expand this research field. This review provides a comprehensive updated overview on the current published work on CAMS using a quantitative approach, focusing on three critical quality attributes of CAMS, i.e., co-formability, physical stability, and dissolution performance. Specifically, co-formability, molar ratio of drug and co-former, preparation methods, physical stability, and in vitro and in vivo performance were covered. For each aspect, a quantitative assessment on the current status was performed, allowing both recent advances and remaining research gaps to be identified. Furthermore, novel research aspects such as the design of ternary CAMS are discussed.

PAT for Continuous Chromatography Integrated into Continuous Manufacturing of Biologics towards Autonomous Operation

This study proposes a reliable inline PAT concept for the simultaneous monitoring of different product components after chromatography. The feed for purification consisted of four main components, IgG monomer, dimer, and two lower molecular weight components of 4.4 kDa and 1 kDa molecular weight. The proposed measurement setup consists of a UV–VIS diode-array detector and a fluorescence detector. Applying this system, a R2 of 0.93 for the target component, a R2 of 0.67 for the dimer, a R2 of 0.91 for the first side component and a R2 of 0.93 for the second side component is achieved. Root mean square error for IgG monomer was 0.027 g/L, for dimer 0.0047 g/L, for side component 1 0.016 g/L and for the side component 2 0.014 g/L. The proposed measurement concept tracked component concentration reliably down to 0.05 g/L. Zero-point fluctuations were kept within a standard deviation of 0.018 g/L for samples with no IgG concentration but with side components present, allowing a reliable detection of the target component. The main reason inline concentration measurements have not been established yet, is the false-positive measurement of target components when side components are present. This problem was eliminated using the combination of fluorescence and UV–VIS data for the test system. The use of this measurement system is simulated for the test system, allowing an automatic fraction cut at 0.05 g/L. In this simulation a consistent yield of &gt;99% was achieved. Process disturbances for processed feed volume, feed purity and feed IgG concentration can be compensated with this setup. Compared to a timed process control, yield can be increased by up to 12.5%, if unexpected process disturbances occur.

Evaluation of Micro-Mechanism and High- and Low-Temperature Rheological Properties of Disintegrated High Volume Crumb Rubber Asphalt (DHVRA).

The aims of this paper are to prepare disintegrated high volume crumb rubber asphalt (DHVRA) with low viscosity, good workability and low-temperature performance by adding disintegrating agent (DA) in the preparation process, and to further analyze the disintegrating mechanism and evaluated high-temperature and low-temperature rheological properties. To obtain DHVRA with excellent comprehensive performance, the optimum DA dosage was determined. Based on long-term disintegrating tests and the Fluorescence Microscopy (FM) method, the correlations between key indexes and crumb rubber (CR) particle diameter was analyzed, and the evaluation indicator and disintegrating stage division standard were put forward. Furthermore, Fourier transform infrared spectroscopy (FT-IR) and Gel Permeation Chromatography (GPC) was used to reveal the reaction mechanism, and the contact angle test method was adopted to evaluate the surface free energy (SFE). In addition, the high-temperature and low-temperature rheological properties were measured, and the optimum CR content was proposed. Results indicated that the optimum DA dosage was 7.5‰, and the addition of DA promoted the melt decomposition of CR, reduced the viscosity and improved the storage stability. The 135 °C rotational viscosity (RV) of DHVRA from mixing for 3 h could be reduced to 1.475 Pa·s, and the softening point difference was even less than 2 °C. The linear correlation between 135 °C RV and the diameter of CR particle in rubber asphalt system was as high as 0.968, and the viscosity decay rate (VDR) was used as the standard to divide the disintegrating process into a fast disintegrating stage, stable disintegrating stage and slight disintegrating stage. Compared to common rubber asphalt (CRA), DHVRA has an absorption peak at 960 cm−1 caused by trans olefin = C-H, and higher molecular weight and polar component of surface energy. Compared with CRA, although the high-temperature performance of DHVRA decreases slightly, the low-temperature relaxation ability can be greatly improved.

Photodegradation of dissolved organic matter of chicken manure: Property changes and effects on Zn2+/Cu2+ binding property

Untreated livestock manure contains high concentrations of dissolved organic matter (DOM), which can enter the environment through leaching and eluviation, showing an important impact on the environment. In this research, fresh chicken manure from a large-scale chicken farm was collected as the source of DOM. The infrared spectrum of the original DOM was characterized. TOC analysis, UV spectrum and 3D fluorescence spectrum were used to measure the properties of DOM before and after photodegradation. Infrared spectroscopy results show that chicken manure DOM may contain aliphatic and aromatic compounds, alcohols, phenols, polysaccharides and some protein substances; In three systems, the order of TOC removal rates of DOM was water + UV system (85%) > > water + simulated sunlight system (7.2%) > ice + simulated sunlight system (4.5%); Changes of UV spectra, fluorescence spectra, molecular weight distribution and pH value show that, in three systems, as the illumination time increased, photodegradation reduced pH value of the systems, aromaticity and humus contents of DOM, while increased the proportion of medium and/or small molecular weight components of DOM. The amounts of all these changes were proportional to DOM photodegradation rates in the system. The binding ability of DOM with Cu2+ and Zn2+ in water solution decreased significantly after the photodegradation.

Simultaneous changes of exogenous dissolved organic matter treated by ozonation in properties and interaction behavior with sulfonamides.

Effluent is often treated with ozone before being discharged into a natural water environment. This process will change the interaction between effluent organic matter and pollutants in aquatic environment. The impact of ozonation on complexation between dissolved organic matter in such wastewater and sulfadimidine often found in natural water was studied in laboratory experiments using four types of real wastewater. Ozonation was found to decrease the proportion of organic matter with a molecular weight greater than 5kDa as well as protein-like, fulvic-like and humic-like components, but except the proportion of hydrophilic components. The aromaticity of the dissolved organic matter was also reduced after ozonation. The complexation of tryptophan and tyrosine with sulfadimidine mainly depends on their hydrophobicity and large molecular weight. Ozonation of fulvic and humic acid tends to produce small and medium molecular weight hydrophilics. The complexation of humic and fulvic acids with sulfadimidine was enhanced by ozonation. Dissolved organic matter, with or without oxidation, were found to weaken sulfadimidine's inhibition of microbial growth, especially for Aeromonas and Acinetobacter species. This finding will expand our understanding about the impact of advanced treatment processes on the dissolved organic matters' properties in effluent.

Elucidation of the mechanism of subunit exchange in \u03b1B crystallin oligomers

AlphaB crystallin (αB-crystallin) is a key protein for maintaining the long-term transparency of the eye lens. In the eye lens, αB-crystallin is a “dynamical” oligomer regulated by subunit exchange between the oligomers. To elucidate the unsettled mechanism of subunit exchange in αB-crystallin oligomers, the study was carried out at two different protein concentrations, 28.5 mg/mL (dense sample) and 0.45 mg/mL (dilute sample), through inverse contrast matching small-angle neutron scattering. Interestingly, the exchange rate of the dense sample was the same as that of the dilute sample. From analytical ultracentrifuge measurements, the coexistence of small molecular weight components and oligomers was detected, regardless of the protein concentration. The model proposed that subunit exchange could proceed through the assistance of monomers and other small oligomers; the key mechanism is attaching/detaching monomers and other small oligomers to/from oligomers. Moreover, this model successfully reproduced the experimental results for both dense and dilute solutions. It is concluded that the monomer and other small oligomers attaching/detaching mainly regulates the subunit exchange in αB-crystallin oligomer.

Novel pervaporation mixed matrix membranes based on polyphenylene isophtalamide modified by metal–organic framework UiO-66(NH2)-EDTA for highly efficient methanol isolation

As a rule, the polymeric membranes have low permeability in separation of low molecular weight components. In spite of this fact, the membrane processes have significant advantages compare with conventional technologies, in particular, low energy consumption and environmental friendliness. To improve transport properties of the polymer membrane their modification should be carried out. In the present work, the development of highly methanol-permeable pervaporation membranes based on poly-m-phenylene isophthalamide (PA) is achieved by two strategies: (i) modification of PA by novel synthesized and characterized highly stable metal–organic framework UiO-66(NH2)-EDTA particles and (ii) development of supported membranes with thin selective layer on the regenerated cellulose substrate. First time the composite structure has been simulated: atomistic molecular dynamics simulations demonstrate the partial penetration of polymer inside the modifier and confirms the nature of the interaction between polymer and modifier assessed by spectroscopic methods. The optimal characteristics in respect of industrial use are obtained for supported PA/UiO-66(NH2)-EDTA (15%) membrane: 1.55 kg/(m2h) permeation flux and 93.1 wt% methanol in the permeate for the separation of azeotropic methanol/toluene mixture.