Component Of Medicine Research Articles

Recent advances in green solvents-based liquid-phase microextraction techniques for chromatographic analysis of active components in traditional Chinese medicine

Recent advances in green solvents-based liquid-phase microextraction techniques for chromatographic analysis of active components in traditional Chinese medicine

A Simple, Cost-Effective, and Sustainable Method for Extraction and Quantification of Chebulagic Acid from Terminalia bellirica (Gaertn.) Roxb. Using RP-HPLC: A Comparative Study

Chebulagic acid (CA), a hydrolysable tannin found in Terminalia bellirica (T. bellirica) (Gaertn.) Roxb., has gained prominence as a significant component in Ayurvedic medicine. This study optimized methods for determining CA in T. bellirica, focusing on fruit samples and prioritizing simplicity, cost-effectiveness, less time duration, and sustainability. We evaluated various extraction techniques, including continuous shaking, microwave assisted extraction, and Soxhlet extraction using green solvents. Quantification of CA was conducted using Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), with confirmation achieved via UV-Visible (UV-Vis) spectroscopy, Thin Layer Chromatography (TLC), and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Results indicated that Soxhlet and microwave significant extraction methods, particularly with ethanol as the green solvent. Soxhlet extraction yielded 49607.73±18.82 μg/mL for fresh fruit and 42952.53±19.09 μg/mL for dry fruit, while microwave extraction resulted in 43627.56±113.48 μg/mL for fresh fruit and 40466.33±130.90 μg/mL for dry fruit respectively. These findings conclude that ethanol as a green solvent in Soxhlet or microwave assisted extraction emerge as most efficient, straightforward, and effective approach for detecting and screening CA in T. bellirica samples.

Integrating Data Mining and Network Pharmacology for Traditional Chinese Medicine for Drug Discovery of Diabetic peripheral neuropathy

The purpose of this study was to examine the therapeutic potential of core traditional Chinese medicine (CTCM) in the treatment of diabetic peripheral neuropathy (DPN) through the use of a data-driven approach that combined network pharmacology and data mining. Important components of traditional Chinese medicine (TCM) and the targets that correspond with them were found through the examination of numerous databases and clinical prescriptions. The possible therapeutic pathways were investigated, with an emphasis on the AGE-RAGE pathway that was discovered via network pharmacology analysis. By evaluating histopathological alterations, inflammatory and apoptotic markers, microcirculation, and blood hypercoagulability in a rat model of DPN, the effectiveness of CTCM was confirmed.Through experimental validation in DPN rats, it was shown that CTCM improved histopathology, decreased inflammation and apoptosis, improved microcirculation, and corrected coagulation abnormalities in addition to alleviating neuropathic pain. These studies show the value of data-driven approaches in advancing traditional medicine research for drug development and offer a mechanistic basis for CTCM's therapeutic potential in DPN.

Pharmacological activities and mechanisms of proteins and peptides derived from traditional Chinese medicine

Abstract Proteins and peptides are important active components of traditional Chinese medicine (TCM) widely found in plants, animals, and fungi. Modern research indicates that the protein constituents of TCM possess various pharmacological activities, including antitumor effect, immunomodulation, antioxidation, antihypertension, anti-inflammation, cardiovascular and nervous system protection, blood sugar regulation, and bacteriostasis effects. Despite significant progress in recent years regarding the pharmacological activities and mechanisms of TCM components, research on protein components of TCM has lagged. Many TCM protein components have yet to be effectively developed, and their pharmacological activities and mechanisms remain insufficiently elucidated. This article provides a comprehensive review of the pharmacological activities and mechanisms of TCM proteins and peptides. Additionally, the current shortcomings in research are discussed to offer some insights, aiming to promote further investigation of TCM proteins and peptides and facilitate their application in new drug development and clinical treatment.

Acupuncture in the Treatment of Male Infertility: A Review

In recent years, there has been a global decline in the quality of human semen, leading to a gradual increase in the incidence of infertility, which significantly impacts men’s health. Due to the complex etiology of male infertility, clinical treatment is predominantly confined to drugs, surgery, and assisted reproductive technology. However, the efficacy of these approaches often falls short of meeting clinical needs, prompting the exploration of new treatment methods. Acupuncture, a crucial component of traditional Chinese medicine (TCM), has gained increasing prominence in the treatment of male infertility, yielding noteworthy results. This article offers an overview of TCM’s perspective on male infertility, explores the clinical efficacy of acupuncture in treating male infertility and the corresponding mechanisms, and summarizes the existing challenges in the current acupuncture-based treatment of male infertility. It also clarifies the validity of acupuncture and provides references for the future development of acupuncture in the treatment of male infertility.

Exercise Response Variation: Avoiding Hysteron Proteron

ABSTRACT Precision exercise medicine is currently one of the most important topics in the clinical exercise physiology field, including exercise-based cardiac rehabilitation (EBCR). A critical component of precision exercise medicine is an appropriate examination for true exercise-associated interindividual response differences (IIRDs) because of EBCR, something that has historically not occurred. As a result, misguided efforts aimed at determining potential moderators and mediators, including genetic interactions and unethical follow-up studies, can occur given that random- and within-subject variation is not accounted for. In this article, a description is provided on how to properly test for true exercise-associated IIRDs in original randomized controlled trials (RCTs) using the standard deviation of individual response (SDIR) approach before deciding on whether an additional examination and/or follow-up studies addressing potential moderators and mediators, including genetic interactions, are necessary. To provide greater readability for a wider audience, we intentionally minimized the use of statistical formulas. It is the hope that the guidance provided in this article will lead to wider adoption of the SDIR approach for examining true IIRDs in original RCTs of EBCR studies as well as spur continued research on this topic. Adherence to the proposed methods will help avoid false conclusions regarding potential moderators and mediators, including genetic interactions, as well as unneeded follow-up studies.

The Role of Panax Notoginseng Saponins on Neuronal Oxidative Stress and Autophagy Response in Craniocerebral Injury Model Rats

This study assessed how IL-6 affects the body’s autophagy status by regulating JAK-STAT3 and explored the mechanism by which IL-6 inhibits JAK-STAT3 signaling pathway-mediated autophagy, and how Panax notoginseng total saponins promote neuronal cell regeneration in rats. A model of neuronal oxidative stress in craniocerebral injury model rats was established, and IL-6 levels were detected by ELISA. Rat neuronal cells were isolated and cultured, while dual-luciferase gene reporter experiments analyzed the targeting relationship between IL-6 and JAK. qRT-PCR detected expression of neuronal autophagy-related genes (JAK, STAT3, ULK1, OsATG7, FAM176A, and Beclin 1). The level of IL-6 in the craniocerebral injury model was significantly higher than that in the control group. IL-6 binds to 3′-UTR of JAK, and transfection with IL-6 inhibitor increases the relative luciferase activity. pMIR-JAK-mut group has no significant effect. There is a targeted regulatory relationship between IL-6 and JAK. Adding total saponins of Panax notoginseng combined with nursing intervention can promote reduction genetic expression related to neuronal autophagy damage. IL-6 can target and regulate JAK gene. IL-6 can promote neuronal development by regulating JAK-STAT3. Autophagy repairs damage and inhibits the autophagy state of cells. Panax notoginseng total saponins are effective medicinal components extracted from high quality panax notoginseng according to extraction and separation technology. The results from this study provide a better understanding of pathogenesis of neuronal oxidative stress in craniocerebral injury model rats and find potential intervention targets, which will provide more treatment methods for neuronal oxidative stress damage in craniocerebral injury model rats.

Serum metabolomics and 16S rRNA amplicon sequencing reveal the role of puerarin in alleviating bone loss aggravated by antidiabetic agent pioglitazone in type 2 diabetic mice

Ethnopharmacological relevancePioglitazone (PIO) was an anti type 2 diabetes (T2D) agent but caused bone loss and bone marrow fat accumulation. Puerarin (PUE) was a natural component of herbal medicine extracted from Pueraria lobata (Willd.) Ohwi and reduced glycemia and improved bone mass as a supplementary drug. A combination of PIO and PUE might be good for maintaining bone mass and blood glucose. Aim of the studyWe aimed to elucidate the potential correlation and underlying mechanisms of dietary supplement PUE in reducing side effects caused by PIO. Materials and methodsIn vitro, alkaline phosphatase (ALP) staining, alizarin S (ARS) staining and qRT-PCR were performed to detect the osteogenesis activity in MC3T3-E1 cells. In vivo, we established the T2D model by treating C57BL6/J mice with high-fat diets and streptozotocin (STZ). Micro-CT, hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAcP) staining were performed to observe the difference in skeletal phenotype. Serum metabolomics and 16S rRNA amplicon sequencing were applied to analyze the potential effect of the combination of PIO and PUE. ResultsWe showed that the PUE could increase ALP activity and mineralization nodes of MC3T3-E1 with PIO. PIO could aggravate bone loss but PUE alleviated the effect caused by PIO in T2D mice. PUE promoted alpha-linolenic acid metabolism and glycerophospholipid metabolism, and affected the alpha diversity of the gut microbiome by regulating the genera of Alloprevotella, Fusobacterium, Rodentibacter, etc. Correlation analysis indicated that sphingosine-1-phosphate, nonadecylic acid, and margaric acid were associated with the effect of PUE. ConclusionsTaken together, we demonstrated that PIO combined with PUE was able to lower blood sugar levels without causing bone loss. The effect of PUE mainly correlated with the genua of Alloprevotella, Fusobacterium, Rodentibacter, and Alistipes. Also, alpha-linolenic acid metabolism and glycerophospholipid metabolism were major targets of PUE.

Value-based healthcare from a military health system perspective: a systematic review

ObjectivesThe aim of this systematic review was to provide an overview of value-based healthcare (VBHC) strategies and/or components within military medicine. For this purpose, the extent to which VBHC has...

Transcriptomics integrated with targeted metabolomics reveals endogenous hormone changes in tuberous root expansion of Pueraria.

Pueraria is a widely cultivated medicinal and edible homologous plant in Asia, and its tuberous roots are commonly used in the food, nutraceutical, and pharmaceutical industries. "Gange No. 5" is a local variety of Pueraria montana var. thomsonii (Bentham) M.R. Almeida (PMT) in Jiangxi Province, China. After optimizing its cultivation technique, we shortened the cultivation cycle of this variety from two years to one year, suggesting that the regulatory mechanism of the endogenous hormone system during tuberous root expansion may have changed significantly. In this study, we focused on the molecular mechanisms of endogenous hormones in promoting tuberous root expansion during one-year cultivation of "Gange No. 5". The mid-late expansion period (S4) is critical for the rapid swelling of "Gange No. 5" tuberous roots during annual cultivation. At S4, the number of cells increased dramatically and their volume enlarged rapidly in the tuberous roots, the fresh weight of a single root quickly increased, and the contents of multiple nutrients (total protein, total phenol, isoflavones) and medicinal components (puerarin, puerarin apigenin, and soy sapogenin) were at their peak values. We compared the transcriptomes and metabolomes of S1 (the pre-expansion period), S4, and S6 (the final expansion period), and screened 42 differentially accumulated hormone metabolites and 1,402 differentially expressed genes (DEGs) associated with hormone biosynthesis, metabolism, and signaling. Most Auxin, cytokinins (CKs), jasmonic acids (JAs), salicylic acid (SA), melatonin (MLT), and ethylene (ETH), reached their maximum levels at S1 and then gradually decreased; however, abscisic acid (ABA) appeared in S6, indicating that most of the endogenous hormones may play a key role in regulating the initiation of tuberous root expansion, while ABA mainly promotes tuberous root maturation. Notably, multiple key genes of the 'Tryptophan metabolism' pathway (ko00380) were significantly differentially expressed, and COBRA1, COBRA2, YUCCA10, IAA13, IAA16, IAA20, IAA27, VAN3, ACAA2, and ARF were also identified to be significantly correlated with the expansion of "Gange No. 5" tuberous roots. Our study has revealed how endogenous hormone regulation affects the expansion of "Gange No. 5" tuberous roots. These findings offer a theoretical foundation for improving the yield of PMT tuberous roots.

Protective Contribution of Rosmarinic Acid in Rosemary Extract Against Copper-Induced Oxidative Stress.

Rosemary extract (Rosmarinus officinalis) is a natural source of bioactive compounds with significant antioxidant properties. Among these, rosmarinic acid is celebrated for its potent antioxidant, anti-inflammatory, antimicrobial, and neuroprotective properties, making it a valuable component in both traditional medicine and modern therapeutic research. Neurodegenerative diseases like Alzheimer's and Parkinson's are closely linked to oxidative damage, and research indicates that rosmarinic acid may help protect neurons by mitigating this harmful process. Rosmarinic acid is able to bind cupric ions (Cu2+) and interfere with the production of reactive oxygen species (ROS) produced by copper through Fenton-like reactions. This study aims to further evaluate the contribution of rosmarinic acid within rosemary extract by comparing its activity to that of isolated rosmarinic acid. By using a detailed approach that includes chemical characterization, antioxidant capacity assessment, and neuroprotective activity testing, we have determined whether the combined components in rosemary extract enhance or differ from the effects of rosmarinic acid alone. This comparison is crucial for understanding whether the full extract offers added benefits beyond those of isolated rosmarinic acid in combating oxidative stress and Aβ-induced toxicity.

Use of SMART objectives in an interprofessional training event: screening for fall and osteoporosis risk

IntroductionSMART objectives are a major component of health coaching and lifestyle medicine, however, there are limited studies describing their use in current health care curriculums. This study incorporated SMART objectives within an Interprofessional Education (IPE) event offered to students who were screening community-dwelling adults within their community for falls and osteoporosis risk. The purpose was to evaluate if students could confidently develop a SMART objective with their client that was relevant to the screening results. MethodsStudent participants were recruited from pharmacy, nursing, and physical therapy programs at Rutgers University. The IPE event consisted of an educational pre-session assignment, 2-h virtual training session, student-led home screening with a self-selected community volunteer, and a 2-h virtual educational and debriefing session. Students taught peers through interprofessional education on their respective discipline's screening assessment tools during each session. Based on the screening results, students collaborated with their clients to develop a SMART objective aimed at addressing an identified problem. ResultsPost 3 yearly programs, 520 pharmacy, nursing, and DPT students completed the SMART objective, and 518 SMART objectives were analyzed. Analysis indicated all students were about 90 % confident in developing SMART objectives and 92 % confident in communicating with clients. Most students, regardless of discipline, felt comfortable developing a SMART objective and communicating with clients. ConclusionA significant number of people screened were found to have risk factors for falls or osteoporosis. Regardless of discipline, students worked with their client to develop appropriate SMART objectives. SMART objectives can enhance IPE programs, curriculums that include client counseling regarding lifestyle changes, and client engagement.

Physicochemical, Phytochemical, NMR, FTIR and LC-MS Analysis of Extract of Ripe Fruits of Carissa carandus L.

Background: Carissa carandus L., commonly known as Karonda, is renowned for its distinctive tangy flavor, making it a valuable ingredient in culinary applications. Additionally, its extensive array of phytoconstituents renders it a significant component of traditional Indian herbal medicine, where it has been used to address various health conditions. Despite its availability, palatability, and diverse phytochemical profile, Karonda remains under utilized, and lacks substantial scientific validation. Further research is needed to substantiate its therapeutic potential and elevate its status in modern scientific and medical contexts. Methods: An ethanolic extract was prepared from the ripe fruits using a hot continuous extraction method. Phytochemical screening of the extract identified the presence of tannins, flavonoids, phenolic compounds, saponins, steroids, and carbohydrates. Subsequent analysis using spectrometric and spectro chromatographic techniques, including NMR, FTIR, and LC-MS, were conducted, supplemented by chromatographic studies, to further elucidate the extract's chemical profile and validate the presence of these bioactive compounds. Results: The analysis of the ripe fruit extract identified several functional groups, including O-H, N-H, C=O, C-N, C-H, and –COOH, based on IR absorption bands observed in the high wave region at 3850 cm−1 and 1728 cm−1. Active compounds were further characterized by comparing these findings with standard reference charts. Proton environments and their electronic states were examined using 1H-NMR spectroscopy. The 1H-NMR spectrum revealed signals at specific δ ppm values corresponding to R-CH3, R-CH2-R, RO-C-H, and F-C-H groups, as detailed in the accompanying table. Conclusion: These results provide a comprehensive chemical fingerprint of CA fruits offering scientific validation of its chemical nature and supporting its potential therapeutic applications.

Biological Characteristics of a Novel Bibenzyl Synthase (DoBS1) Gene from Dendrobium officinale Catalyzing Dihydroresveratrol Synthesis.

Bibenzyl compounds are one of the most important bioactive components of natural medicine. However, Dendrobium officinale as a traditional herbal medicine is rich in bibenzyl compounds and performs functions such as acting as an antioxidant, inhibiting cancer cell growth, and assisting in neuro-protection. The biosynthesis of bibenzyl products is regulated by bibenzyl synthase (BBS). In this study, we have cloned the cDNA gene of the bibenzyl synthase (DoBS1) from D. officinale using PCR with degenerate primers, and we have identified a novel type III polyketide synthase (PKS) gene by phylogenetic analyses. In a series of perfect experiments, DoBS1 was expressed in Escherichia coli, purified and some catalytic properties of the recombinant protein were investigated. The molecular weight of the recombinant protein was verified to be approximately 42.7 kDa. An enzyme activity analysis indicated that the recombinant DoBS1-HisTag protein was capable of using 4-coumaryol-CoA and 3 malonyl-CoA as substrates for dihydroresveratrol (DHR) in vitro. The Vmax and Km of the recombinant protein for DHR were 3.57 ± 0.23 nmol·min-1·mg-1 and 0.30 ± 0.08 mmol, respectively. The present study provides further insights into the catalytic mechanism of the active site in the biosynthetic pathway for the catalytic production of dihydroresveratrol by bibenzylase in D. officinale. The results can be used to optimize a novel biosynthetic pathway for the industrial synthesis of DHR.

Multi-Indicator Comprehensive Quality Evaluation of Turpinia arguta (Lindl.) Seem Herbs at Different Harvesting Periods

This study aimed to investigate differences in the leaf biomasses, chemical compositions, and pharmacological activities of Turpinia arguta histocultured seedlings and live seedlings at different harvesting periods (S1, S2, S3, and S4) in order to determine the optimal harvesting period. Eleven indexes, including biomass, key active components, secondary metabolites, nutrient content, and antioxidant activity, were evaluated by high-performance liquid chromatography and colorimetric methods during different harvesting periods. The weights of the 11 indexes were calculated by principal component analysis, and then a comprehensive quality evaluation was performed. The results showed significant differences in leaf biomasses, key active components, secondary metabolites, nutrient contents, and antioxidant activities between the different harvesting periods. The highest quality score was obtained for the S4 period, indicating that the quality of T. arguta was the best at this time and that the S4 period was the most suitable harvesting period. At this period (S4), the medicinal component content, antioxidant activity, and comprehensive quality score of the histocultured seedlings of T. arguta were higher than those of the live seedlings, indicating the importance of tissue culture technology in enhancing the quality of T. arguta. This study provides more novel and abundant information and reference for determining the appropriate harvesting period of T. arguta, with the aim of providing newer scientific guidance for the management of herbs.

Medical Practitioner’s experiential knowledge of the roles of Medical Social Workers at the Benjamin Mkapa Hospital, Tanzania

Medical social work is focused on improving patient treatment results by analyzing the bio-psycho-social-spiritual components of medicine. However, medical practitioners in Tanzanian hospitals do not acknowledge medical social workers' functions in healthcare environments. This study, which investigates the roles of medical social workers in Tanzanian hospitals, has the potential to significantly impact the development of new task-sharing and health policies. Using mixed methods, 94 practitioners filled out questionnaires and interviewed 11 key informants. The study revealed that nearly three-quarters of the respondents, 66(70%) indicate that the role of hospital social workers is the mental health practitioner. More than half of the respondents that is 64(68%), indicate that hospital social workers provide patient and family counseling, 58(62%) indicate providing emotional support to patients and families, and 54(57%) indicate facilitating decision-making on behalf of patients and families. Five major themes were identified: connecting patients with members of the community, performing patients' financial analysis, maintaining the social welfare of patients and hospital staff, handling cases of abuse and violence, and diagnosing the psychosocial-spiritual problems of patients. These findings will enable groups of healthcare professionals to rationally reorganize medical social workers’ roles and improve patients’ treatment outcomes in Tanzania hospitals.

Exploring Spiritual Care Competency in Palliative Medicine: A Narrative Inquiry of Physician Care Notes on Spiritual Distress.

Background: Spiritual care is a critical component of hospice and palliative medicine; however, physicians frequently lack the necessary competencies to adequately address patients' spiritual needs. This study aims to examine the spiritual care competencies of physicians as reflected in their care notes, thereby providing insights into their approaches for managing spiritual distress. Methods: A narrative inquiry approach was employed to qualitatively analyze interaction notes from 30 palliative care patients experiencing spiritual distress. Data were collected from a medical university professor's care notes between April 2019 and February 2024. Results: The most common form of spiritual distress observed was reluctance to leave life, followed by loss of dignity. Physicians demonstrated expertise in holistic care, addressing physical, psychological, and spiritual needs with a comprehensive approach. Their demeanor reflects acceptance of mortality and acknowledgment of the limitations of medical care, helping foster gratitude and contentment in patients. Effective communication was pivotal, involving the conveyance of information, empathetic engagement, and active listening. Compassionate interactions emphasized the importance of spiritual care in patient-centered care delivery. Conclusions: Spiritual care competencies are essential for improving patient outcomes and advancing comprehensive palliative care. Future efforts should integrate these competencies into communication training within medical education to more effectively enhance health care providers' competencies.

Injury prevention and rehabilitation strategies in physical education: A machine learning-based approach using biomechanical characteristics

The field of sports biomechanics employs concepts from physics, biology, and engineering to investigate the mechanical characteristics of human motion and how they affect the body’s anatomy and functions. With the development of technology, sports biomechanics has emerged as a crucial component of sports medicine, training, and rehabilitation. To reduce the risk of injury and enhance athletic performance, sports biomechanics examines motions in sports in great length. The purpose of the study is to establish injury prevention and rehabilitation strategies for physical education (PE) teaching based on biomechanical characteristics. The early warning mode of sports injuries is recognized using advanced deep learning (DL) techniques, specifically resilient convolutional neural networks (RCNN). Biomechanical data from wearable sensors is used in this study to find trends related to sports-related injuries. A questionnaire survey of 228 students from various colleges was conducted. Individualized rehabilitation strategies will be provided to injured participants, taking into account their unique biomechanical deficiencies. These programs will be created in conjunction with physical therapists, and they will be updated in response to the patient’s progress toward recovery. The study found that their sports injuries were acute and chronic. This research demonstrated the treatment, prevention, and rehabilitation strategies of injuries in sports. The study emphasizes that biomechanical analysis is crucial for improving PE programs, which will eventually enhance students’ performance and overall health.

Carrier-free nanomedicine for combined antibacterial therapy through pH-responsive controlled release of sulfadiazine and emodin

To mitigate bacterial drug resistance and enhance antibacterial efficacy in the treatment of bacterial infections, a novel antibacterial self-releasing nanomedicine was developed in this study. The innovative nanodrug integrates the active components of traditional Chinese medicine with antibiotics to circumvent bacterial resistance. Additionally, phenylboronic acid (PBA) was used as a bacterial targeting agent to specifically localize the infection site and regulate drug release. In this system, sulfadiazine (Su), emodin (Em), and PBA were assembled through boron ester bonds and hydrophobic interactions, forming a stable self-delivery nanodrug designated as Su/Em/PBA. Subsequently, the drug release capability was evaluated at pH 5.5 and pH 7.4. Notably, the boronic acid ester bond formed by PBA is pH-responsive, demonstrating a significantly higher release of Su and Em in the acidic microenvironment of bacterial infections (pH 5.5) compared to physiological environment (pH 7.4) (Su: 93.9 % vs. 29.9 %; Em: 81.9 % vs. 16.4 %). This indicated that Su/Em/PBA exhibited an effective drug release capability at the site of bacterial infection. The bactericidal effect was evaluated through in vitro plate staining experiments and live/dead bacterial staining assays. The combination of Su and Em enhanced the antibacterial efficacy of Su/Em/PBA, achieving antibacterial rates of 84.0 % against Staphylococcus aureus (S. aureus) and 88.6 % against Escherichia coli (E. coli), signifying the effective antibacterial properties of Su/Em/PBA. In conclusion, our study presents a straightforward approach to developing antibacterial self-releasing nanomedicine with excellent antibacterial activity, making it a promising candidate for future clinical trials.

Advances and Challenges of Microneedle Assisted Drug Delivery for Biomedicals Applications: A Review.

Microneedles have been explored as a novel way of delivering active ingredients into the skin. They have various advantages, such as quick and efficient drug delivery, mechanical stability, minimal pain, variable capacity and easy use. Microneedles are enabled for the delivery of vaccine, peptides, medicinal components and in cosmetology, which couldn't go unnoticed. The novel approaches in the transdermal drug delivery system have increased the efficiency of drug delivery into the skin by crossing the skin barriers. This platform has a wide range of applications and can also be used to deliver non-transdermal biomedicals. The variety in the design of microneedles has demanded similar diversity in their methods of fabrication; micro molding and drawing lithography may be useful methods. There are different types of polymers and materials for the fabrication of microneedles. Several synthetic and natural materials are used in the fabrication of microneedles. Unique shapes, materials, and mechanical properties are modified for organ-specific applications in microneedle engineering. In this review, we discuss several factors and their roles to cross the biological barriers for transdermal drug delivery in various sites, such as in ocular, vascular, oral, and mucosal tissue. Additionally, this article highlights the future scope of transdermal drug delivery systems through microneedles.