Component Of Response Research Articles

Living in the ‘Blue Zone’ of a sea-level rise inundation map: Community perceptions of coastal flooding in King Salmon, California

As sea-level rise (SLR) inundation maps proliferate, it is important to study their politics – both how they are created and how they act upon and shape various lives and places. This paper uses the example of King Salmon, CA – a rural, low-income residential area projected to be one of the most at risk to SLR on the US West Coast – to examine how a community responds to external projections showing SLR risk to their homes and businesses. Through interviews with 17 King Salmon community members and observation of a county-hosted ‘communities at risk’ workshop, we examined the community’s social context, their past experiences with flooding, and their reaction to SLR projection maps including what next steps they would like to see taken. Residents expressed a strong connection to the place, noting that it is one of the few affordable places to live on the coast in California. We found that residents already live with regular flooding during larger tides of the year and have taken steps to adapt. We observed a strong generational component in responses to projection maps with many older respondents believing or hoping that the worst effects from SLR would not come until after they passed away. Residents expressed a lack of faith in government to address flooding concerns both at present and into the future, noting that general maintenance issues have gone unaddressed for decades. Many residents interviewed and observed seemed open or at least resigned to the possibility of relocation at a future undetermined time. This work reveals the power dynamics inherent in climate projections like SLR maps, which, due to their technical nature and mobility, can leave communities out of conversations related to potential futures. Findings also have implications related to climate and SLR work – highlighting the importance of understanding community context; contributing to equity considerations about how wealth and other demographic factors shape how communities interact with SLR planning; and spotlighting the need for sustained learning, engagement, and co-production with communities in the ‘blue zones’ of SLR inundation maps.

Changes in visual function in long‐term type 1 diabetic patients without diabetic retinopathy using full‐field electroretinogram

Aims/Purpose: To evaluate changes in retinal function in type 1 diabetic patients (DM1) without diabetic retinopathy (DR) using full‐field electroretinogram (ffERG).Methods: Forty‐six eyes of long term DM1 patients without DR were compared to 46 eyes of healthy subjects. None of them had any ophthalmic pathology that could alter the findings. All the subjects were evaluated following the standardized international ISCEV guidelines. The amplitude and implicit time (IT) of each component of scotopic and photopic responses were evaluated, along with interocular differences.Results: There were no age differences between groups. A significant increase in the rod response IT of the DM1 patients was observed compared to the control group (p = 0.0012). The b wave amplitude in the mixed response was statistically reduced in the DM1 group compared to the control group (p = 0.03). There was an increase in the IT of both a and b‐wave in the mixed response at the higher stimulus intensity in the DM1 compared to control (p = 0.023 and p = 0.004 respectively). b/a rate was similar in both groups. The oscillatory potentials (OPs) showed an increase in their IT (p = 0.008) and a decrease in OP2 amplitude in the DM1 group. Cone mediated b‐wave was diminished in DM1.Conclusions: Full‐field electroretinogram enables the detection of subclinical findings in long term DM1 patients suggesting the existence of neurodegeneration prior the appearance of DR, particularly in the rod system and OPs.

Exploring the evolution of T cell function and diversity across different stages of non-small cell lung cancer.

The immune system plays a key role in detecting and fighting cancerous tumors. T cells are a crucial component in both natural and therapeutic cancer immunoediting responses, but it is unclear if they are the primary agents of these processes. In this study, patients with lung lesions detected by CT scan were selected, and their peripheral blood samples were analyzed for T cell population and serum cytokines/chemokines. T cell subtypes (CD3, CD4, CD8, CD27, CD28, CD45, CD45RA, CD57, CCR7, and PD1) and serum cytokines/chemokines (IL-2, IL-6, IL-10, IFN-γ, TGF-β, TNFα, CXCL1, CXCL9, and CXCL12) were measured by flow cytometry and analysis before surgical resection or other cancer treatments. The frequency of T cell subpopulations in patients with lung cancer (n = 111) corresponded to those seen in patients with T cell exhaustion. As lung cancer progressed, the proportion of effector memory T cells decreased, while the proportion of naive T cells, PD-1, CD57+, CD28+CD27+, CD45RA+, and CD3+CD4+CCR7 increased. Circulating CD8+PD1+ T cells were positively correlated with intra-tumoral PD-L1 expression. Concurrently, serum levels of IL-2, TGF-β, and CXCL9 decreased, while IL-6, IL-10, IFN-γ, and CXCL12 increased during the progression of lung cancer. In conclusion, T cell dysfunction is associated with cancer progression, particularly in advanced-stage lung cancer, and cancer immunoediting will provide early-stage cancer detection and further therapeutic strategies.

Mass Decontamination of Companion Dogs in Disaster: Planning for Personnel, Water, and Time Requirements.

The importance of companion animals in the daily lives of people, and the increasing incidence and severity of natural disasters impacting people and their animals, is very well documented. However, despite the advancement of companion animal response capabilities, decontamination remains an inconsistently implemented component of disaster response. The challenge for local authorities is their need for planning factors and protocols specific to companion animal decontamination which are generally lacking. Data is provided on the average time requirements, water use and containment resources necessary, and the personnel required to decontaminate (decon) a large number of companion dogs. Sixty-three lightly contaminated, medium weight, short to medium coat, highly tractable dogs (Labradors and Hounds) from a State facility colony were used to determine the water requirements, soap effectiveness, and time required to complete decon (washing/bathing). Data were collected over a 6-mo period using 2 personnel that were randomly assigned to wash the dogs. Difference in weight, bathing time, and water use between groups was evaluated using a 2-tailed 2-sample t-test for independent data. The time and water requirements were significantly different between medium coated dogs and short coated dogs. On average, for a short coated dog, the amount of time to complete decon was 7 min, and the amount of water was 8-10 gal. For medium coated dogs, the time increased to 10-12 min to complete the process and 12-15 gal water. The results of this study provide important insights emergency management planners, animal response team members, and community personnel tasked with implementation of mass decontamination of companion dogs following a natural or man-made disaster.

Multiple Genes Core to ERAD, Macroautophagy and Lysosomal Degradation Pathways Participate in the Proteostasis Response in α1-Antitrypsin Deficiency

BackgroundIn the classical form of α1-antitrypsin deficiency (ATD), the misfolded α1-antitrypsin Z (ATZ) variant accumulates in the endoplasmic reticulum (ER) of liver cells. A gain-of-function proteotoxic mechanism is responsible for chronic liver disease in a sub-group of homozygotes. Proteostatic response pathways, including conventional ERAD and autophagy, have been proposed as the mechanisms that allow cellular adaptation and presumably protection from the liver disease phenotype. Recent studies have concluded that a distinct lysosomal pathway called ERLAD completely supplants the role of the conventional macro-autophagy pathway in degradation of ATZ. Here we used several state-of-the-art approaches to more fully characterize the proteostatic responses in cellular systems that model ATD. MethodsWe used CRISPR-mediated genome editing coupled to a cell selection step by FACS to carry out screening for proteostasis genes that regulate ATZ accumulation and combined that with selective genome editing in two other model systems. ResultsERAD genes are key early regulators and multiple autophagy genes, from classical as well as from ERLAD and other newly described ER-phagy pathways, participate in degradation of ATZ in a manner that is temporally regulated and evolves as ATZ accumulation persists. Time-dependent changes in gene expression are accompanied by specific ultrastructural changes including dilation of the ER, formation of globular inclusions, budding of autophagic vesicles and alterations in the overall shape and component parts of mitochondria. ConclusionsMacro-autophagy is a critical component of the proteostasis response to cellular ATZ accumulation and it becomes more important over time as ATZ synthesis continues unabated. Multiple sub-types of macro-autophagy and non-autophagic lysosomal degradative pathways are needed to respond to the high concentrations of misfolded protein that characterizes ATD and these pathways are attractive candidates for genetic variants that predispose to the hepatic phenotype.

Jasmonic acid improves barley photosynthetic efficiency through a possible regulatory module, MYC2-RcaA, under combined drought and salinity stress.

The combined stress of drought and salinity is prevalent in various regions of the world, affects several physiological and biochemical processes in crops, and causes their yield to decrease. Photosynthesis is one of the main processes that are disturbed by combined stress. Therefore, improving the photosynthetic efficiency of crops is one of the most promising strategies to overcome environmental stresses, making studying the molecular basis of regulation of photosynthesis a necessity. In this study, we sought a potential mechanism that regulated a major component of the combined stress response in the important crop barley (Hordeum vulgare L.), namely the Rubisco activase A (RcaA) gene. Promoter analysis of the RcaA gene led to identifying Jasmonic acid (JA)-responsive elements with a high occurrence. Specifically, a Myelocytomatosis oncogenes 2 (MYC2) transcription factor binding site was highlighted as a plausible functional promoter motif. We conducted a controlled greenhouse experiment with an abiotic stress-susceptible barley genotype and evaluated expression profiling of the RcaA and MYC2 genes, photosynthetic parameters, plant water status, and cell membrane damages under JA, combined drought and salinity stress (CS) and JA + CS treatments. Our results showed that applying JA enhances barley's photosynthetic efficiency and water relations and considerably compensates for the adverse effects of combined stress. Significant association was observed among gene expression profiles and evaluated physiochemical characteristics. The results showed a plausible regulatory route through the JA-dependent MYC2-RcaA module involved in photosynthesis regulation and combined stress tolerance. These findings provide valuable knowledge for further functional studies of the regulation of photosynthesis under abiotic stresses toward the development of multiple-stress-tolerant crops.

Discrimination of object information by bat echolocation deciphered from acoustic simulations.

High-precision visual sensing has been achieved by combining cameras with deep learning. However, an unresolved challenge involves identifying information that remains elusive for optical sensors, such as occlusion spots hidden behind objects. Compared to light, sound waves have longer wavelengths and can, therefore, collect information on occlusion spots. In this study, we investigated whether bats could perform advanced sound sensing using echolocation to acquire a target's occlusion information. We conducted a two-alternative forced choice test on Pipistrellus abramus with five different targets, including targets with high visual similarity from the front, but different backend geometries, i.e. occlusion spots or textures. Subsequently, the echo impulse responses produced by these targets, which were difficult to obtain with real measurements, were computed using three-dimensional acoustic simulations to provide a detailed analysis consisting of the acoustic cues that the bats obtained through echolocation. Our findings demonstrated that bats could effectively discern differences in target occlusion spot structure and texture through echolocation. Furthermore, the discrimination performance was related to the differences in the logarithmic spectral distortion of the occlusion-related components in the simulated echo impulse responses. This suggested that the bats obtained occlusion information through echolocation, highlighting the advantages of utilizing broadband ultrasound for sensing.

The Sub-Metered HVAC Implemented for Demand Response Dataset

Abstract Closed-loop control of commercial building heating, ventilating, and air conditioning (HVAC) for demand response requires measurements used as feedback to the controllers. Demand response effectiveness is usually measured as a power deviation from baseline, but the building automation system (BAS) does not usually collect power measurements, and whole-building electric meters typically measure power at intervals of 15 min, which may be too slow for some types of demand response. Demand response strategies are sometimes focused on components of building HVAC systems, e.g., the response of supply/return fans to temperature set-point changes, but these components are usually not submetered. Fan power can be estimated from physics-based models leveraging BAS data, e.g., airflow measurements; but our ability to effectively close the loop on these estimates is not clear. In this paper, we introduce a massive dataset that contains both submetered fan power data and BAS data for several building HVAC systems during typical operation and demand response events. Through a case study we show that models leveraging BAS data alone do not provide accurate estimates of fan power during event transients, making it unlikely that closed-loop control of commercial building HVAC components for demand response would be effective using BAS data alone. This demonstrates the value of submetering HVAC components. More broadly, our dataset will enable future research bridging the gap between building control and power systems research.

The non-pathogenic protozoon Leishmania tarentolae interferes with the activation of NLRP3 inflammasome in human cells: new perspectives in the control of inflammation.

Innate immune responses against infectious agents can act as triggers of inflammatory diseases. On the other hand, various pathogens have developed mechanisms for the evasion of the immune response, based on an inhibition of innate immunity and inflammatory responses. Inflammatory diseases could thus be controlled through the administration of pathogens or pathogen-derived molecules, capable of interfering with the mechanisms at the basis of inflammation. In this framework, the NLRP3 inflammasome is an important component in innate antimicrobial responses and a major player in the inflammatory disease. Parasites of the genus Leishmania are master manipulators of innate immune mechanisms, and different species have been shown to inhibit inflammasome formation. However, the exploitation of pathogenic Leishmania species as blockers of NLRP3-based inflammatory diseases poses safety concerns. To circumvent safety issues associated with pathogenic parasites, we focused on Leishmania tarentolae, a species of Leishmania that is not infectious to humans. Because NLRP3 typically develops in macrophages, in response to the detection and engulfment microorganisms, we performed our experiments on a monocyte-macrophage cell line (THP-1), either wild type or knockout for ASC, a key component of NLRP3 formation, with determination of cytokines and other markers of inflammation. L. tarentolae was shown to possess the capability of dampening the formation of NLRP3 inflammasome and the consequent expression of pro-inflammatory molecules, with minor differences compared to effects of pathogenic Leishmania species. The non-pathogenic L. tarentolae appears a promising pro-biotic microbe with anti-inflammatory properties or a source of immune modulating cellular fractions or molecules, capable of interfering with the formation of the NLRP3 inflammasome.

Presentation of a Social Responsibility Measurement Model in Tejarat Bank with a Brand-Centric Approach

Objective: Nowadays, social responsibility is considered essential for organizations, as it significantly influences various organizational outputs. Many organizations are continually striving to improve their image through the development of their social responsibilities. This study presents a model for measuring social responsibility in Tejarat Bank. Methodology: The research was conducted using a mixed-method approach (qualitative and quantitative). The qualitative sample consisted of 10 experts, and the quantitative sample included 413 managers and specialists from Tejarat Bank, with 203 individuals selected as the statistical sample using a sample size formula. In the qualitative part, an initial model including 3 dimensions, 9 components, and 45 indicators was extracted through interviews with bank experts. Subsequently, the extracted model was tested using structural equation modeling in the LISREL software environment. Findings: Results showed three main dimensions: customer-centricity (including components of responsiveness and service quality), legal and ethical (including stakeholders, employees, community rights, and organizational activities), and environmental focus (including environmental factors, online marketing, and internet service provision). Conclusion: To improve service quality, it is suggested that managers examine the customer experience in all service delivery processes and assess customers' mental satisfaction. It is also recommended that service delivery channels to customers be standardized, meaning that services received through various means (such as mobile banking, internet banking, etc.) should be uniform, and if a customer begins a service process through one channel, they should be able to continue it through another.

Potential involvement of neutrophils on exercise effects in breast cancer malignancy.

This review aimed to comprehensively explore and elucidate multifaceted neutrophils in breast cancer, particularly in the context of physical activity. Neutrophils play a critical role in the tumor microenvironment and systemic immune response, despite their short half-life and terminal differentiation. Through a thorough review of research related to changes in immunity in breast cancer during exercise, this review aims to provide comprehensive insights into immunological changes, especially focusing on neutrophils. Recognizing that much of the existing research has predominantly focused on T cells and nature killer (NK) cells, our review seeks to shift the spotlight toward understanding how exercise affects neutrophils, a less-explored but critical immune response component in breast cancer. This study involved an extensive review of the literature (from 2000 to 2023) using the PubMed, Science Direct, and Google Scholar databases. The keywords chosen for the searches were "immune cells and exercise," "exercise and breast cancer," "tumor microenvironment and neutrophils," and "neutrophils and exercise and breast cancers." Neutrophils in the tumor microenvironment can exhibit distinct phenotypes and functions. These differences have yielded conflicting results regarding tumor progression. Exercise plays a positive role in breast cancer and alters the immune system. Physical activity can quantitatively and functionally regulate neutrophils under various conditions such as metabolic disruption or senescence. This short communication outlines exercise-induced neutrophil diversification and its role in breast cancer progression, both within and systemically within the tumor microenvironment. Exercise may provide benefits through the potential neutrophil involvement in breast cancer.

Effect of bisphenol A on formation and process of alergic reaction - review

One of the possible immunomodulatory effects of BPA is alterating the allergy process and asthma. The growth in the prevalence of asthma around the world roughly corresponds with the rise in the widespread usage of certain chemicals. Despite the fact that asthma is a complicated and varied condition brought on by exposure to a variety of environmental factors and genetic predispositions, many classes of chemicals that are frequently present in the surrounding environment and are linked to endocrine disruption have recently been linked to the disease's pathogenesis. BPA is endocrine disrupting chemical compound made up of two phenol rings joined by a methyl bridge and two methyl functional groups adhered to the bridge. BPA is ingested by humans, inhaled via home dust, and exposed through the skin. An allergy is an aberrant immunological reaction to an antigen, such as a protein or allergen. An ingested or breathed allergen encounters the epithelium as its initial barrier. The inflammatory condition and increased permeability of the epithelial barrier are associated with allergic inflammatory disorders, and this suggests that the epithelium barrier is more sensitive to allergen sensitization. The existing literature and scientific publications do not adequately address the direct influence of bisphenols on the development and influence of the allergic process. Multiple authors discuss how bisphenol affects specific allergic response components. We may conclude that exposure to bisphenol A alters the allergic response based on the findings of the studies and the analysis of the literature.

The yeast-human coevolution: Fungal transition from passengers, colonizers, and invaders.

Fungi are the cause of more than a billion infections in humans every year, although their interactions with the host are still neglected compared to bacteria. Major systemic fungal infections are very unusual in the healthy population, due to the long history of coevolution with the human host. Humans are routinely exposed to environmental fungi and can host a commensal mycobiota, which is increasingly considered as a key player in health and disease. Here, we review the current knowledge on host-fungi coevolution and the factors that regulate their interaction. On one hand, fungi have learned to survive and inhabit the host organisms as a natural ecosystem, on the other hand, the host immune system finely tunes the response toward fungi. In turn, recognition of fungi as commensals or pathogens regulates the host immune balance in health and disease. In the human gut ecosystem, yeasts provide a fingerprint of the transient microbiota. Their status as passengers or colonizers is related to the integrity of the gut barrier and the risk of multiple disorders. Thus, the study of this less known component of the microbiota could unravel the rules of the transition from passengers to colonizers and invaders, as well as their dependence on the innate component of the host's immune response. This article is categorized under: Infectious Diseases > Environmental Factors Immune System Diseases > Environmental Factors Infectious Diseases > Molecular and Cellular Physiology.

A digital twin-based framework for damage detection of a floating wind turbine structure under various loading conditions based on deep learning approach

Engineering has many necessary fields, and Structural Health Monitoring (SHM) is one of the most important of them. Sometimes in industrial environments, it is difficult and even impossible to collect data containing different real damages. Therefore, the problem of data acquisition represents a primary challenge in designing damage detection systems. The application of digital twin methods based on simulated models and/or Machine Learning (ML) models is a practical way to solve this problem. In this approach, a digital twin is generated for a compromised structure, utilizing a physics-based model to analyze diverse damage scenarios. Subsequently, an ML model is trained using data extracted from the physics-based model, functioning as the digital twin. This research proposes a method based on a digital twin for detecting damages in structures. The data produced from a Floating Wind Turbine (FWT) model was used to evaluate the performance of the proposed digital twin-based method. For this purpose, the FWT structure was simulated using a numerical model to address the data collection problem in the face of various uncertainties, such as changing loading conditions. In line with the concept of digital twin and to reduce the computational time, a Deep Convolution Long Short-Term Memory Neural Network (DCLSTMNN) model was designed and trained only with the frequency data of various scenarios of the simulated FWT model under constant loads (deterministic loads, including constant wind speed and airy wave model) to learn the damage-sensitive features. Then, to demonstrate the robustness of the proposed model under different uncertainties, the DCLSTMNN model was evaluated using the frequency data of the simulated FWT model under variable loading conditions (including Kaimal wind model and JONSWAP wave theory). Some vibration response components unrelated to the nature of the FWT model were removed using the Complete Ensemble Empirical Mode Decomposition (CEEMD) method. Then, the reconstructed vibration responses were used to create the frequency data using the Frequency Domain Decomposition (FDD) technique. The study results show that the proposed digital twin-based method can detect the location and severity of damage more accurately than other comparable methods despite various uncertainties.

Squaramides enhance NLRP3 inflammasome activation by lowering intracellular potassium

The NLRP3 inflammasome is a component of the inflammatory response to infection and injury, orchestrating the maturation and release of the pro-inflammatory cytokines interleukin-1β (IL-1β), IL-18, and triggering pyroptotic cell death. Appropriate levels of NLRP3 activation are needed to avoid excessive tissue damage while ensuring host protection. Here we report a role for symmetrical diarylsquaramides as selective K+ efflux-dependent NLRP3 inflammasome enhancers. Treatment of macrophages with squaramides potentiated IL-1β secretion and ASC speck formation in response to K+ efflux-dependent NLRP3 inflammasome activators without affecting priming, endosome cargo trafficking, or activation of other inflammasomes. The squaramides lowered intracellular K+ concentration which enabled cells to respond to a below-threshold dose of the inflammasome activator nigericin. Taken together these data further highlight the role of ion flux in inflammasome activation and squaramides as an interesting platform for therapeutic development in conditions where enhanced NLRP3 activity could be beneficial.

Palmitic acid, but not other long-chain saturated fatty acids, increases S100B protein and TNF-α secretion by astrocytes

Obesity is a health problem that involves fat accumulation in adipose and other tissues and causes cell dysfunction. Long-chain saturated fatty acids can induce and propagate inflammation, which may also contribute to the brain alterations found in individuals with obesity. Fatty acids accumulate in astrocytes in situations of blood‒brain barrier disruption, such as inflammatory conditions. Furthermore, the increase in tumor necrosis factor-alpha (TNF-α) and S100 calcium-binding protein B (S100B) secretion is considered an essential component of the inflammatory response. We hypothesize that through their action on astrocytes, long-chain saturated fatty acids mediate some of the brain alterations observed in individuals with obesity. Here, we investigate the direct effect of long-chain fatty acids on astrocytes. Primary astrocyte cultures were incubated for 24 hours with myristic, palmitic, stearic, linoleic, or α-linolenic acids (25–100 µM). All saturated fatty acids tested led to an increase in TNF-α secretion, but only palmitic acid, one of the most common fatty acids, increased S100B secretion, indicating that S100B secretion is probably not caused in response to TNF-α release. Palmitic acid also caused nuclear migration of nuclear factor kappa B. Long-chain saturated fatty acids did not alter cell viability or redox status. In conclusion, long-chain saturated fatty acids can alter astrocytic homeostasis and may contribute to brain disorders associated with obesity, such as neuroinflammation.

RAF-like protein kinases mediate a deeply conserved, rapid auxin response

The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants and algae. The nuclear auxin pathway mediates gene expression and controls growth and development in land plants, but this pathway is absent from algal sister groups. Several components of rapid responses have been identified in Arabidopsis, but it is unknown if these are part of a conserved mechanism. We recently identified a fast, proteome-wide phosphorylation response to auxin. Here, we show that this response occurs across 5 land plant and algal species and converges on a core group of shared targets. We found conserved rapid physiological responses to auxin in the same species and identified rapidly accelerated fibrosarcoma (RAF)-like protein kinases as central mediators of auxin-triggered phosphorylation across species. Genetic analysis connects this kinase to both auxin-triggered protein phosphorylation and rapid cellular response, thus identifying an ancient mechanism for fast auxin responses in the green lineage.

Cellular mechanisms of taste disturbance induced by the non-steroidal anti-inflammatory drug, diclofenac, in mice.

Drug-induced taste disorders are a serious problem in an aging society. This study investigated the mechanisms underlying taste disturbances induced by diclofenac, a non-steroidal anti-inflammatory drug that reduces pain and inflammation by inhibiting the synthesis of prostaglandins by cyclooxygenase enzymes (COX-1 and COX-2). RT-PCR analyses demonstrated the expression of genes encoding arachidonic acid pathway components such as COX-1, COX-2 and prostaglandin synthases in a subset of mouse taste bud cells. Double-staining immunohistochemistry revealed that COX-1 and cytosolic prostaglandin E synthase (cPGES) were co-expressed with taste receptor type-1 member-3 (T1R3), a sweet/umami receptor component, or gustducin, a bitter/sweet/umami-related G protein, in a subset of taste bud cells. Long-term administration of diclofenac reduced the expression of genes encoding COX-1, gustducin and cPGES in mouse taste buds and suppressed both the behavioral and taste nerve responses to sweet and umami taste stimuli but not to other tastants. Furthermore, diclofenac also suppressed the responses of both mouse and human sweet taste receptors (T1R2/T1R3, expressed in HEK293 cells) to sweet taste stimuli. These results suggest that diclofenac may suppress the activation of sweet and umami taste cells acutely via a direct action on T1R2/T1R3 and chronically via inhibition of the COX/prostaglandin synthase pathway inducing down-regulated expression of sweet/umami responsive components. This dual inhibition mechanism may underlie diclofenac-induced taste alterations in humans.

The impact of crossmodal predictions on the neural processing of aesthetic stimuli.

Neuroaesthetic research has focused on neural predictive processes involved in the encounter with art stimuli or the related evaluative judgements, and it has been mainly conducted unimodally. Here, with electroencephalography, magnetoencephalography and an affective priming protocol, we investigated whether and how the neural responses to non-representational aesthetic stimuli are top-down modulated by affective representational (i.e. semantically meaningful) predictions between audition and vision. Also, the neural chronometry of affect processing of these aesthetic stimuli was investigated. We hypothesized that the early affective components of crossmodal aesthetic responses are dependent on the affective and representational predictions formed in another sensory modality resulting in differentiated brain responses, and that audition and vision indicate different processing latencies for affect. The target stimuli were aesthetic visual patterns and musical chords, and they were preceded by a prime from the opposing sensory modality. We found that early auditory-cortex responses to chords were more affected by valence than the corresponding visual-cortex ones. Furthermore, the assessments of visual targets were more facilitated by affective congruency of crossmodal primes than the acoustic targets. These results indicate, first, that the brain uses early affective information for predictively guiding aesthetic responses; second, that an affective transfer of information takes place crossmodally, mainly from audition to vision, impacting the aesthetic assessment. This article is part of the theme issue 'Art, aesthetics and predictive processing: theoretical and empirical perspectives'.

Antiviral Activity of Zinc Finger Antiviral Protein (ZAP) in Different Virus Families.

The CCCH-type zinc finger antiviral protein (ZAP) in humans, specifically isoforms ZAP-L and ZAP-S, is a crucial component of the cell's intrinsic immune response. ZAP acts as a post-transcriptional RNA restriction factor, exhibiting its activity during infections caused by retroviruses and alphaviruses. Its function involves binding to CpG (cytosine-phosphate-guanine) dinucleotide sequences present in viral RNA, thereby directing it towards degradation. Since vertebrate cells have a suppressed frequency of CpG dinucleotides, ZAP is capable of distinguishing foreign genetic elements. The expression of ZAP leads to the reduction of viral replication and impedes the assembly of new virus particles. However, the specific mechanisms underlying these effects have yet to be fully understood. Several questions regarding ZAP's mechanism of action remain unanswered, including the impact of CpG dinucleotide quantity on ZAP's activity, whether this sequence is solely required for the binding between ZAP and viral RNA, and whether the recruitment of cofactors is dependent on cell type, among others. This review aims to integrate the findings from studies that elucidate ZAP's antiviral role in various viral infections, discuss gaps that need to be filled through further studies, and shed light on new potential targets for therapeutic intervention.