Complex Integration Research Articles

Abstract We136: HnRNPA1 Modulates Expression of Myogenic Differentiation Factors by Maintenance of Ppp1r1b - lncRNA /PRC2 Complex Integrity

Objective: HnRNPA1 is an important member of the HNRNP family, serves multiple functions. The long noncoding RNA Ppp1r1b is a key regulator of myogenesis in cardiac and skeletal muscle development. This study aimed to investigate the role of hnRNPA1 in Ppp1r1b-lncRNA mediated myogenesis and identify the underlying molecular mechanisms. Methods: Ppp1r1b-lncRNA : protein complex was isolated by RNA pulldown assay. The corresponding proteins were identified by mass spectrometry. The binding sites of hnRNPA1 and EZH2 on Ppp1r1b-lncRNA were further evaluated by RNA pulldown assay and western blotting. After downregulation of hnRNPA1, expression of muscle development related genes was measured by qRT-PCR and western blot analysis. Furthermore, EZH2 RIP (RNA Immunoprecipitation), H3K27me3 CHIP (Chromatin Immunoprecipitation) and Ppp1r1b-lncRNA CHIRP (Chromatin Isolation by RNA Purification) were applied. Results: HnRNPA1 and EZH2 both bind to Ppp1r1b-lncRNA and their bindng sites are located at different positions. EZH2 RIP assay and H3K27me3 CHIP show decreased Ppp1r1b-lncRNA /EZH2 interaction and corresponded decreased H3K27me3 modification after downregulation of hnRNPA1. The expression of myogenic diffrentiation related genes was significantly suppressed by hnRNPA1-RNAi while CHIRP assay indicated elevated interaction of Ppp1r1b-lncRNA with Myod1 and Myogenin promoter. Conclusions: HnRNPA1 facilitates the integrity of Ppp1r1b-lncRNA /PRC2 complex, which preserves correct regulation of myogenesis gene expression. After hnRNPA1 suppression, the elevated promoter occupancy of Ppp1r1b- lncRNA corresponds with transcriptional repression. The hnRNPA1/ Ppp1r1b lncRNA /PRC2 complex regulatory axis is a potential therapeutic target during heart and skeletal muscle development.

Proximity ligation-based sequencing for the identification of human papillomavirus genomic integration sites in formalin-fixed paraffin embedded oropharyngeal squamous cell carcinomas.

Human papillomavirus (HPV) infections are an increasing cause of oropharyngeal squamous cell carcinomas (OPSCC). Integration of the viral genome into the host genome is suggested to affect carcinogenesis, however, the correlation with OPSCC patient prognosis is still unclear. Research on HPV integration is hampered by current integration detection technologies and their unsuitability for formalin-fixed paraffin-embedded (FFPE) tissues. This study aims to develop and validate a novel targeted proximity-ligation based sequencing method (targeted locus amplification/capture [TLA/TLC]) for HPV integration detection in cell lines and FFPE OPSCCs. For the identification of HPV integrations, TLA/TLC was applied to 7 cell lines and 27 FFPE OPSCCs. Following preprocessing steps, a polymerase chain reaction (PCR)-based HPV enrichment was performed on the cell lines and a capture-based HPV enrichment was performed on the FFPE tissues before paired-end sequencing. TLA was able to sequence up to hundreds of kb around the target, detecting exact HPV integration loci, structural variants, and chromosomal rearrangements. In all cell lines, one or more integration sites were identified, in accordance with detection of integrated papillomavirus sequences PCR data and the literature. TLC detected integrated HPV in 15/27 FFPE OPSCCs and identified simple and complex integration patterns. In general, TLA/TLC confirmed PCR data and detected additional integration sites. In conclusion TLA/TLC reliably and robustly detects HPV integration in cell lines and FFPE OPSCCs, enabling large, population-based studies on the clinical relevance of HPV integration. Furthermore, this approach might be valuable for clonality assessment of HPV-related tumors in clinical diagnostics.

A related-event approach to event integration in Japanese complex predicates: iconicity, frequency, or efficiency?

A related-event approach to event integration in Japanese complex predicates: iconicity, frequency, or efficiency?

Synergy and antagonism in the integration of BCR and CD40 signals that control B-cell proliferation.

In response to infection or vaccination, a successful antibody response must enrich high-affinity antigen-reactive B-cells through positive selection, but eliminate auto-reactive B-cells through negative selection. B-cells receive signals from the B-cell receptor (BCR) which binds the antigen, and the CD40 receptor which is stimulated by neighboring T-cells that also recognize the antigen. How BCR and CD40 signaling are integrated quantitatively to jointly determine B-cell fate decision and proliferation remains unclear. To investigate this, we developed a differential-equations-based model of the BCR and CD40 signaling networks activating NFκB. Our model accurately recapitulates the NFκB dynamics of B-cells stimulated through their BCR and CD40 receptors, correctly predicting that costimulation induces more NFκB activity. However, when linking it to established cell fate decision models of cell survival and cell cycle control, it predicted potentiated population expansion that was not observed experimentally. We found that this discrepancy was due to a time-dependent functional antagonism exacerbated by BCR-induced caspase activity that can trigger apoptosis in founder cells, unless NFκB-induced survival gene expression protects B-cells in time. Guided by model predictions, sequential co-stimulation experiments revealed how the temporal dynamics of BCR and CD40 signaling control the fate decision between negative and positive selection of B-cell clonal expansion. Our quantitative findings highlight a complex non-monotonic integration of BCR and CD40 signals that is controlled by a balance between NFκB and cell-death pathways, and suggest a mechanism for regulating the stringency of B-cell selection during an antibody response.

Oxidative Stress and Mitochondria Are Involved in Anaphylaxis and Mast Cell Degranulation: A Systematic Review.

Anaphylaxis, an allergic reaction caused by the massive release of active mediators, can lead to anaphylactic shock (AS), the most severe and potentially life-threatening form of anaphylactic reaction. Nevertheless, understanding of its pathophysiology to support new therapies still needs to be improved. We performed a systematic review, assessing the role and the complex cellular interplay of mitochondria and oxidative stress during anaphylaxis, mast cell metabolism and degranulation. After presenting the main characteristics of anaphylaxis, the oxidant/antioxidant balance and mitochondrial functions, we focused this review on the involvement of mitochondria and oxidative stress in anaphylaxis. Then, we discussed the role of oxidative stress and mitochondria following mast cell stimulation by allergens, leading to degranulation, in order to further elucidate mechanistic pathways. Finally, we considered potential therapeutic interventions implementing these findings for the treatment of anaphylaxis. Experimental studies evaluated mainly cardiomyocyte metabolism during AS. Cardiac dysfunction was associated with left ventricle mitochondrial impairment and lipid peroxidation. Studies evaluating in vitro mast cell degranulation, following Immunoglobulin E (IgE) or non-IgE stimulation, revealed that mitochondrial respiratory complex integrity and membrane potential are crucial for mast cell degranulation. Antigen stimulation raises reactive oxygen species (ROS) production from nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and mitochondria, leading to mast cell degranulation. Moreover, mast cell activation involved mitochondrial morphological changes and mitochondrial translocation to the cell surface near exocytosis sites. Interestingly, antioxidant administration reduced degranulation by lowering ROS levels. Altogether, these results highlight the crucial role of oxidative stress and mitochondria during anaphylaxis and mast cell degranulation. New therapeutics against anaphylaxis should probably target oxidative stress and mitochondria, in order to decrease anaphylaxis-induced systemic and major organ deleterious effects.

Impacts of operating variables in forward roll coating process of viscous hybrid nanofluid

Abstract Roll coating plays a significant role in various coating industries such as magnetic records, wallpapers, wrapping, adhesive tapes, books and magazines, photographic and plastic films. The thin layer coating of a magnetohydrodynamic (MHD) viscous hybrid nanofluid by passing through the space between two co-rotating rolls has been studied in an isothermal and incompressible analysis. The governing equation of mass and momentum are obtained then dimensionless using lubrication approximation theory (LAT). The velocity, pressure gradient, and pressure distribution are determined by the exact solution. Using Simpson’s (3/8) rule for numerical integration, the complex integral is examined. Important engineering parameters including power and roll separating force delivered by the rolls to the fluid are also estimated numerically. Raising the volume fraction of nanoparticles raises the pressure distribution and pressure gradient while having little effect on the velocity profile. It seems that the magnetic field and hybrid nanofluid, both seem very advantageous for the efficient roll coating process, controlling the separation force, power input, and distance between the attachment and separation point.

Dysferlin Enables Tubular Membrane Proliferation in Cardiac Hypertrophy.

Cardiac hypertrophy compensates for increased biomechanical stress of the heart induced by prevalent cardiovascular pathologies but can result in heart failure if left untreated. Here, we hypothesized that the membrane fusion and repair protein dysferlin is critical for the integrity of the transverse-axial tubule (TAT) network inside cardiomyocytes and contributes to the proliferation of TAT endomembranes during pressure overload-induced cardiac hypertrophy. Stimulated emission depletion and electron microscopy were used to localize dysferlin in mouse and human cardiomyocytes. Data-independent acquisition mass spectrometry revealed the cardiac dysferlin interactome and proteomic changes of the heart in dysferlin-knockout mice. After transverse aortic constriction, we compared the hypertrophic response of wild-type versus dysferlin-knockout hearts and studied TAT network remodeling mechanisms inside cardiomyocytes by live-cell membrane imaging. We localized dysferlin in a vesicular compartment in nanometric proximity to contact sites of the TAT network with the sarcoplasmic reticulum, a.k.a. junctional complexes for Ca2+-induced Ca2+ release. Interactome analyses demonstrated a novel protein interaction of dysferlin with the membrane-tethering sarcoplasmic reticulum protein juncophilin-2, a putative interactor of L-type Ca2+ channels and ryanodine receptor Ca2+ release channels in junctional complexes. Although the dysferlin-knockout caused a mild progressive phenotype of dilated cardiomyopathy, global proteome analysis revealed changes preceding systolic failure. Following transverse aortic constriction, dysferlin protein expression was significantly increased in hypertrophied wild-type myocardium, while dysferlin-knockout animals presented markedly reduced left-ventricular hypertrophy. Live-cell membrane imaging showed a profound reorganization of the TAT network in wild-type left-ventricular myocytes after transverse aortic constriction with robust proliferation of axial tubules, which critically depended on the increased expression of dysferlin within newly emerging tubule components. Dysferlin represents a new molecular target in cardiac disease that protects the integrity of tubule-sarcoplasmic reticulum junctional complexes for regulated excitation-contraction coupling and controls TAT network reorganization and tubular membrane proliferation in cardiomyocyte hypertrophy induced by pressure overload.

Additive Technologies and Materials for the Next‐Generation CubeSats and Small Satellites

AbstractCubeSat and small satellites play a very important role in modern space exploration. Their success and diverse capabilities rely on the development of efficient and compact sub‐systems. This task is not trivial due to multiple challenges posed by their small size and mass, and the solution calls for conceptually new designs for the fabrication and integration of complex miniaturized satellite components. The importance of additive techniques in small satellite manufacturing is steadily increasing as they enable rapid, large‐scale production of sophisticated architectures, such as hollow, webbed parts with a cell‐like structure similar to animal bone, with lower mass and improved functionality. Moreover, these architectures feature higher strength, enhanced heat transfer, and efficient thermal and electromagnetic radiation shielding. When compared to traditional subtractive technologies like cutting and milling, additive manufacturing proves to be more versatile and effective in realizing architectures with an increasing intricacy of shapes, structures, and compositions. The perspective explores the suitability of 3D printing in various satellite production tasks, including the propulsion system components and satellite elements. Looking ahead, the challenges and advantages of integrating 3D printing technology into satellite production, emphasizing the need for continuous development through consolidated, proactive collaborative efforts of many devoted teams are outlined.

Complex and rational hypergeometric functions on root systems

We consider some new limits for the elliptic hypergeometric integrals on root systems. After the degeneration of elliptic beta integrals of type I and type II for root systems An and Cn to the hyperbolic hypergeometric integrals, we apply the limit ω1→−ω2 for their quasiperiods (corresponding to b→i in the two-dimensional conformal field theory) and obtain complex beta integrals in the Mellin–Barnes representation admitting exact evaluation. Considering type I elliptic hypergeometric integrals of a higher order obeying nontrivial symmetry transformations, we derive their descendants to the level of complex hypergeometric functions and prove the Derkachov–Manashov conjectures for functions emerging in the theory of non-compact spin chains. We describe also symmetry transformations for a type II complex hypergeometric function on the Cn-root system related to the recently derived generalized complex Selberg integral. For some hyperbolic beta integrals we consider a special limit ω1→ω2 (or b→1) and obtain new hypergeometric identities for sums of integrals of rational functions.

Efficient computation of highly oscillatory finite-part integrals

This study is devoted to the numerical evaluation of finite-part integrals given by⨎0+∞xαf(x)(x−τ)m+1S(ωx)dx,τ>0,α>−1,m∈N, where S(ωx) represents highly oscillatory functions of the form S(ωx)=Hν(1)(ωx) and S(ωx)=eiωx with ω≫1. Through singularity separation, we introduce a uniform approximation scheme utilizing the complex integration method. The study includes an analysis of error estimates and convergence rates, demonstrating the superior effectiveness of the proposed algorithm for handling oscillatory integrands. Plentiful numerical results highlight the stability and efficiency of the developed schemes.

Connected FPSOs Thrive on Automation

Modern life is connected. Our computers, phones, and watches are all tapped into personal insights, from a daily planner to keep track of meetings and appointments to a health monitor tracking everything from heart rate to blood oxygen level in real time. This connectivity has bled over into many different facets of our lives, including work. Business and industry maintain critical connections allowing for simplification of complex tasks and the monitoring of machines for safety and performance. As the oil field has become more connected, increasing opportunities for automation have been introduced into both new and existing oilfield technologies with aims to achieve new levels of productivity and safety. Whether it is a robotic arm on the drill floor of a rig bringing pipe from the stand to the well or autonomous robots being used for inspections and other operation observations in hard-to-reach or remote places, if the connectivity is there it opens the door to a vast, new toolkit for contractors and operators alike. In 2023, a group of contractors came together to help define the next steps in automation for floating production, storage, and offloading (FPSO) vessels. The FPSO Coalition—made up of SLB, Rockwell Automation, Sensia, and Cognite—is a collaboration designed to accelerate the evolution of FPSOs by developing and employing new digital capabilities aimed at improving the reliability, availability, safety, and efficiency of the assets, while lowering the carbon footprint of their offshore operations. FPSOs have been invaluable tools for offshore development around the world for decades. Some older hardware does not have the benefit of being wired for connection or pieces of equipment being Internet of Things (IoT)-ready. As much as the coalition looks to solutions to integrate into new facilities, it also explores the limitations and potential for bringing older yet still viable components into the digital age. Under the venture, SLB was tapped to bring advanced digital solutions, subsurface domain expertise, and complex integration and project management capabilities to scale up efficiencies and lower the carbon footprint of operations across every stage of the FPSO life cycle. Rockwell would provide expertise in control and safety systems for FPSOs, as well as extensive experience in power systems. “Keeping in mind that the typical operating life of an FPSO can be 20-25-30 years, a lot can change in terms of technology, process conditions, regulatory requirements, etc.,” said Greg Trostel, global industry development manager for floating production at Rockwell Automation. “But ultimately, the main goal of automation is to ensure the reliable and safe operation of the vessel—no loss of containment, no personnel at risk, no environmental incidents. “Ideally, automation systems can adapt and grow with the changing technology and changing requirements. We depend on the automation systems to keep all the individual process modules connected to, and operating in concert with, the main vessel systems,” he said. One of the first things tasked to the coalition was a contract awarded to SLB for a multipurpose dynamic simulation solution providing an immersive digital twin environment to dynamically simulate operational processes and their control and automation systems, train personnel, and strengthen safety elements at five newbuild FPSO units to be installed in Búzios Field in the Santos Basin for Petrobras.

Ultrasonic Healing of Plastrons.

Superhydrophobic surfaces (SHS) exhibit a pronounced ability to resist wetting. When immersed in water, water does not penetrate between the microstructures of the SHS. Instead, a thin layer of trapped gas remains, i.e., plastron. This fractional wetting is also known as the Cassie-Baxter state (CB). Impairment of superhydrophobicity occurs when water penetrates the plastron and, when complete wetting is achieved, a Wenzel state (W) results. Subsequent recovery back to CB state is one of the main challenges in the field of SHS wetting. Current methods for plastron recovery require complex mechanical or chemical integration, are time-consuming or lack spatial control. Here an on-demand, contact-less approach for performing facile transitions between these wetting states at micrometer length scales is proposed. This is achieved by the use of acoustic radiation force (ARF) produced by high-intensity focused ultrasound (HIFU). Switching from CB to W state takes <100 µs, while the local recovery back to CB state takes <45 s. To the best of authors knowledge, this is the first demonstration of ARF-induced manipulation of the plastron enabling facile two-way controlled switching of wettingstates.

Parameterization of Physiologically Based Biopharmaceutics Models: Workshop Summary Report.

This Article shares the proceedings from the August 29th, 2023 (day 1) workshop "Physiologically Based Biopharmaceutics Modeling (PBBM) Best Practices for Drug Product Quality: Regulatory and Industry Perspectives". The focus of the day was on model parametrization; regulatory authorities from Canada, the USA, Sweden, Belgium, and Norway presented their views on PBBM case studies submitted by industry members of the IQ consortium. The presentations shared key questions raised by regulators during the mock exercise, regarding the PBBM input parameters and their justification. These presentations also shed light on the regulatory assessment processes, content, and format requirements for future PBBM regulatory submissions. In addition, the day 1 breakout presentations and discussions gave the opportunity to share best practices around key questions faced by scientists when parametrizing PBBMs. Key questions included measurement and integration of drug substance solubility for crystalline vs amorphous drugs; impact of excipients on apparent drug solubility/supersaturation; modeling of acid-base reactions at the surface of the dissolving drug; choice of dissolution methods according to the formulation and drug properties with a view to predict the in vivo performance; mechanistic modeling of in vitro product dissolution data to predict in vivo dissolution for various patient populations/species; best practices for characterization of drug precipitation from simple or complex formulations and integration of the data in PBBM; incorporation of drug permeability into PBBM for various routes of uptake and prediction of permeability along the GI tract.

Paths of Least Resistance: Unconventional Effector Secretion by Fungal and Oomycete Plant Pathogens.

Effector secretion by different routes mediates the molecular interplay between host plant and pathogen, but mechanistic details in eukaryotes are sparse. This may limit the discovery of new effectors that could be utilized for improving host plant disease resistance. In fungi and oomycetes, apoplastic effectors are secreted via the conventional endoplasmic reticulum (ER)-Golgi pathway, while cytoplasmic effectors are packaged into vesicles that bypass Golgi in an unconventional protein secretion (UPS) pathway. In Magnaporthe oryzae, the Golgi bypass UPS pathway incorporates components of the exocyst complex and a t-SNARE, presumably to fuse Golgi bypass vesicles to the fungal plasma membrane. Upstream, cytoplasmic effector mRNA translation in M. oryzae requires the efficient decoding of AA-ending codons. This involves the modification of wobble uridines in the anticodon loop of cognate tRNAs and fine-tunes cytoplasmic effector translation and secretion rates to maintain biotrophic interfacial complex integrity and permit host infection. Thus, plant-fungal interface integrity is intimately tied to effector codon usage, which is a surprising constraint on pathogenicity. Here, we discuss these findings within the context of fungal and oomycete effector discovery, delivery, and function in host cells. We show how cracking the codon code for unconventional cytoplasmic effector secretion in M. oryzae has revealed AA-ending codon usage bias in cytoplasmic effector mRNAs across kingdoms, including within the RxLR-dEER motif-encoding sequence of a bona fide Phytophthora infestans cytoplasmic effector, suggesting its subjection to translational speed control. By focusing on recent developments in understanding unconventional effector secretion, we draw attention to this important but understudied area of host-pathogen interactions. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Reconstruction of the paleotemperature of the Central Yakutia Ice Complex during the Holocene period

The increase in worldwide temperatures is leading to changes in permafrost landscapes in disturbed areas, which are causing the onset of cryogenic processes. Central Yakutia has a high population density and human impact on the permafrost, which contribute to the widespread occurrence of these processes. Studying the changes in permafrost over past periods is crucial for predicting future landscape changes, monitoring soil temperature changes in current warming conditions, and evaluating the stability of the upper permafrost layer. We reconstructed the temperature regime of the soils in the Ice Complex using mathematical modeling based on the chronology of climate evolution in Central Yakutia during the Holocene. Mathematical modeling of the temperature regime of the ice complex’s soils is based on climatic and thermophysical characteristics of the soils selected from the lithological sections of Central Yakutia. Archive data from weather stations was used for the analysis and sampling of climatic characteristics such as average annual and monthly air temperatures and snow cover thickness. During periods of warming, the calculated ground temperatures at the depth of zero heat turnover were between –2.1 and –2.7 °C, showing a difference of 0.4–1 °C from the current temperatures. Conversely, during cooling periods, the temperatures ranged from –5.2 to –12.3 °C, with a variance of 2.1–9.2 °C from the current soil temperatures. Thus, the examination of computational models indicates that the thermal conditions of the soils within the Ice Complex in Central Yakutia during the most significant periods of the Holocene are impacted by the thickness of the snow cover and the integrity of the Ice Complex.

Cross-linguistic perception of Spanish intonation by Chinese speakers: Effects of linguistic experience and prosodic features

This study aims to explore the cross-linguistic perception of Spanish intonation patterns and the role of linguistic experience and prosodic features in identifying the question-statement contrasts. To this end, 43 Spanish native listeners and 75 Chinese learners of Spanish were presented with auditory stimuli in a gating paradigm for the recognition of sentence types. The results showed that listeners’ perception of target intonational contours was highly dependent on their language experience. Although Spanish listeners outperformed Chinese learners in perceiving different intonational cues, most Chinese learners were able to correctly identify sentence types after hearing the initial F0 peak due to the positive transfer of their tonal processing ability in their native language. In addition, our data revealed that the position of the final stressed syllable might also be a relevant factor for discriminating the sentence type depending on the length of the utterance. Overall, the findings seem to suggest a complex integration mechanism for cross-linguistic intonation perception, in which listeners’ language specificity and the prosodic structures of the materials should be accounted for evaluating non-native auditory performance.

A simple electronic medical record-based predictors of illness severity in sepsis (sepsis) score.

Current scores for predicting sepsis outcomes are limited by generalizability, complexity, and electronic medical record (EMR) integration. Here, we validate a simple EMR-based score for sepsis outcomes in a large multi-centre cohort. A simple electronic medical record-based predictor of illness severity in sepsis (SEPSIS) score was developed (4 additive lab-based predictors) using a population-based retrospective cohort study. Internal medicine services across four academic teaching hospitals in Toronto, Canada from April 2010-March 2015 (primary cohort) and 2015-2019 (secondary cohort). We identified patients admitted with sepsis based upon receipt of antibiotics and positive cultures. The primary outcome was in-hospital mortality and secondary outcomes were ICU admission at 72 hours, and hospital length of stay (LOS). We calculated the area under the receiver operating curve (AUROC) for the SEPSIS score, qSOFA, and NEWS2. We then evaluated the SEPSIS score in a secondary cohort (2015-2019) of hospitalized patients receiving antibiotics. Our primary cohort included 1,890 patients with a median age of 72 years (IQR: 56-83). 9% died during hospitalization, 18.6% were admitted to ICU, and mean LOS was 12.7 days (SD: 21.5). In the primary and secondary (2015-2019, 4811 patients) cohorts, the AUROCs of the SEPSIS score for predicting in-hospital mortality were 0.63 and 0.64 respectively, which were similar to NEWS2 (0.62 and 0.67) and qSOFA (0.62 and 0.68). AUROCs for predicting ICU admission at 72 hours, and length of stay > 14 days, were similar between scores, in the primary and secondary cohorts. All scores had comparable calibration for predicting mortality. An EMR-based SEPSIS score shows a similar ability to predict important clinical outcomes compared with other validated scores (qSOFA and NEWS2). Because of the SEPSIS score's simplicity, it may prove a useful tool for clinical and research applications.

Poetic Metaphors Related to Birds: A Conceptual Blending in Puisi-Puisi Cinta, WS. Rendra

Metaphors are commonly used to vividly convey deeply abstract human emotions. This is because human emotions are indeed an abstract – conceptual. In the poem of Puisi-Puisi Cinta, the poet employs a great deal of emotional poetic metaphors relating to birds in order to communicate the variety of feelings that are experienced by the characters, such as features, roles, and visual aspects. This paper aims to study the emotional poetic metaphors related to birds in Puisi-Puisi Cinta using conceptual blending theory. The research data in the study consists the poetic metaphors related to Birds in Puisi – Pusi Cinta by WS Rendra. The data are conducted qualitatively using the blending theory. The blending theory’s G.Fauconnier and M.Turner (2002) came to the conclusion that metaphorizing is not limited by the projection from “the source domain” to “the target domain”, but includes complex dynamic integration processes, creating new blended mental spaces, which are able to create the meaning structure. The findings reveal that these vivid poetic metaphors in Puisi-Puisi Cinta by W.S Rendra are substantial through the use of imagery depicting aspects of the birds' appearances and its features to human beings. The metaphorical process, which projects from the birds to human beings, can be seen as a process of conceptual blending. In addition, each and every poetic metaphor related to birds of the poem has its characteristics in a certain cognitive model.

Theoretical design of a single-mode fiber-based bi-order Bessel beam for a STED system

Stimulated emission depletion (STED) microscopy has attracted great research attention due to its applications to breaking diffraction limits for imaging and lithography. However, its implementation based on single-mode fibers often encounters challenges such as complex structural integration, costly fabrication processes, and the need for specific fiber designs. Herein, a low-cost bi-order Bessel beam based on one single-mode fiber integrated with a structurally simple wavelength-scale microstructure (WSM) on fiber end was proposed for STED system. Through simulation study for full-scale WSM optimization, we have successfully developed a bicolor laser beam (BLB) consisting of a zero-order Bessel beam at a wavelength of 405 nm and a donuts high-order Bessel beam at a wavelength of 532 nm. This fiber-based configuration allows us to achieve a diffraction-limited spot size with a working distance of 0.67λpump and a minimum FWHM of 0.395λpump. By combining wavelength division multiplexing technology with power modulation of the donuts beam, this work provides a promising way for achieving super-resolution imaging or lithography with only one single-mode fiber.

ASSOCIATION BETWEEN WEIGHTBEARING CT AND MRI FINDINGS IN PROGRESSIVE COLLAPSING FOOT DEFORMITY

BackgroundWeightbearing computed tomography scans allow for better understanding of foot alignment in patients with Progressive Collapsing Foot Deformity. However, soft tissue integrity cannot be assessed via WBCT. As performing both WBCT and magnetic resonance imaging is not cost effective, we aimed to assess whether there is an association between specific WBCT and MRI findings.MethodsA cohort of 24 patients of various stages of PCFD (mean age 51±18 years) underwent WBCT scans and MRI. In addition to signs of sinus tarsi impingement, four three-dimensional measurements (talo-calcaneal overlap, talo-navicular coverage, Meary's angle axial/lateral) were obtained using a post processing software (DISIOR 2.1, Finland) on the WBCT datasets. Sinus tarsi obliteration, spring ligament complex and tibiospring ligament integrity, as well as tibialis posterior tendon degeneration were evaluated with MRI. Statistical analysis was performed for significant (P&lt;0.05) correlation between findings.ResultsNone of the assessed 3D measurements correlated with spring ligament complex or tibiospring ligament tears. Age, body mass index, and TCO were associated with tibialis posterior tendon tears. 75% of patients with sinus tarsi impingement on WBCT also showed signs of sinus tarsi obliteration on MRI. Of the assessed parameters, only age and BMI were associated with sinus tarsi obliteration diagnosed on MRI, while the assessed WBCT based 3D measurements were, with the exception of MA axial, associated with sinus tarsi impingement.ConclusionWhile WBCT reflects foot alignment and indicates signs of osseous impingement in PCFD patients, the association between WBCT based 3D measurements and ligament or tendon tears in MRI is limited. Partial or complete tears of the tibialis posterior tendon were only detectable in comparably older and overweight PCFD patients with an increased TCO. WBCT does not replace MRI in diagnostic value. Both imaging options add important information and may impact decision-making in the treatment of PCFD patients.