Ventilation cooling design is one of the key technologies during the design of large hydrogenerator. With the increase of hydrogenerator capacity, the overheating problem of rotor region become more and more serious. In this paper, a 250 MW hydrogenerator is analyzed. The transient electromagnetic field of the hydrogenerator is calculated. The losses (heat sources) of rotor components in the rotor region of the hydrogenerator are determined. Three-dimensional fluid and thermal coupled mathematic model of the hydrogenerator rotor region is established. The rotor rotation of the hydrogenerator is considered. The distribution of complex fluid velocity in the rotor region is calculated using the finite volume method. The influence of fluid velocity in the different directions on the temperature of the rotor excitation winding is studied under the different flow rates in the rotor region. The surface heat-transfer coefficient distribution of the rotor components is determined. The temperature distribution of the rotor excitation winding, rotor pole body, rotor press plate, rotor damping bar, and rotor end ring is obtained. The calculated temperature results match well with test values. These provide an important reference for the rotor structural design and optimization of larger hydrogenerator.