Completely Randomized Design Research Articles

Methods of Breaking Seed Dormancy for Four Species of Ornamental Cacti

The aim of this study was to investigate methods for breaking the seed dormancy of cacti that are cultivated in Thailand. To stimulate seed germination and decrease the production time for cacti, the research used four cacti species: Astrophytum asterias, Echinocactus grusonii, Myrtillocactus geometrizans, and Turbinicarpus alonsoi, which are extensively grown as ornamental cacti species in Thailand. The experimental design used in this study was a completely randomized design (CRD). The pot experiment consisted of 4 dormancy breaking methods and six replicates, 30 seeds per pot. For the control, seeds were sown onto planting media directly. In the experimental samples, seeds were subjected to different pre-soaking treatments: soaking for 24 h in room temperature water (28 2oC), GA3 500 ppm for 24 h, and H2SO4 (50%) for 30 min. The seed germination and plant growth data were collected after sowing cacti seeds for two months. The results showed that soaking cacti seeds in room temperature water produced the highest germination rates for A. asterias, E. grusonii, and M. geometrizans. In addition, this treatment also reduced the production time of M. geometrizans. Concurrently, A. asterias, E. grusonii, and M. geometrizans presented higher stem diameters and root lengths of the seedlings, as well as the best result on the seedling vigor of cacti. Soaking seeds with GA3 resulted in the highest germination percentage of T. alonsoi and accelerated seed germination of A. Asterias, and T. alonsoi. Moreover, this treatment led to the maximum root lengths and seedling vigor of T. alonsoi. However, soaking seeds with H2SO4 was a treatment that gave E. grusonii the maximum root length and seedling vigor.

Evaluation of Growing Media and Chemical Treatment on the Seedling Growth of High Chill Peach

The present study entitled “Evaluation of Growing Media and Dormancy Breaking Chemical on the Seedling Growth of High Chill Peach” was conducted at Dept. of Horticulture, College of Agriculture and Horticulture Research Centre, G.B.P.U.A&T, Pantnagar, Uttarakhand during the year 2022-23. The experiments were carried out in Factorial Complete Randomized Design (FCRD) with 3 replications per treatment. Under seedling growth studies, the total numbers of treatment were 16 with 2 factors comprising of four growing media (soil: sand: FYM, soil: sand: vermicompost, soil: sand: cocopeat and soil: FYM: vermicompost: cocopeat) and four chemical treatments (control, GA3 @ 500 ppm, kinetin @ 50 ppm and thiourea @ 5000 ppm). In the experiment the maximum seedling length, shoot fresh weight, shoot dry weight, root length, root fresh weight and root dry weight was recorded in soil: FYM: vermicompost: cocopeat, GA3 @ 500 ppm and interaction M4C3 (soil: FYM: vermicompost: cocopeat + GA3 @ 500ppm) while the minimum values were recorded in soil: sand: cocopeat, control and M3C1. Result from this study discovered that GA3 @ 500 ppm along with the growing media soil: FYM: vermicompost: cocopeat recorded the maximum seedling length, diameter, number of leaves, leaf area, shoot fresh weight and shoot dry weight which can be recommended for raising the rootstocks for the production of quality planting material of peach for seedling rootstock.

The combination of nanoparticles and endophytes boosts Thyme (Thymus vulgaris L.) resistance to drought stress by elevating levels of phenolic compounds, flavonoids, and essential oils

BackgroundAs climate change and water scarcity increasingly threaten agricultural productivity, enhancing plant resilience to drought has attracted great attention. This study explored the potential of combining Fe2O3 nanoparticles (FeNPs), endophytic bacteria (EB), and endophytic fungi (EF) to boost drought tolerance in Thymus vulgaris. The research aimed to assess how these combined treatments affect the plant’s physiological responses and chemical composition under drought stress.ResultsA factorial experiment was designed using completely randomized design (CRD) method, incorporating four irrigation levels [100%, 75%, 50%, and 25% field capacity (FC)], four FeNPs concentrations (0, 0.5, 1, and 1.5 mg L−1), and three endophyte types (control, bacteria, and fungi). After extracting, purifying, identifying, and screening EB and EF from nine Lamiaceae species, the endophytes Azospirillum lipoferum and Aspergillus oryzae isolated from Salvia mirzayanii exhibited the highest drought resistance. The highest amounts of TFM (45.45 g) and TDM (21.56 g) were obtained using the combination of EB and FeNPs at 1 mg L−1 under irrigation with 100% FC. At 25% FC, EB treatment increased the activities of polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) by 62.12% and 18.23% compared to the control, respectively. In addition, under optimal humidity conditions, FeNP concentrations were higher than 0.5 mg L−1 leading to a decrease in PPO activity. At 25% FC, the highest content of total phenols was observed with a 37.5% increase under EB treatment using 1 mg L−1 of FeNPs, while the highest content of total flavonoids showed a 62.72% increase under EF treatment with 0.5 mg/L of FeNPs. The highest level of p-coumaric acid was obtained under EF (34.27% compared to control). At 25% FC, EB and FeNPs (1 mg L−1) increased the level of caffeic acid in thyme plants by 24.70% and 10.08%, respectively. In addition, inoculation with EB increased (11.9%) the content of ferulic acid in plants and the application of FeNPs as a foliar spray decreased the level of ferulic acid in thyme plants. The highest essential oil percentage at 25% FC was observed after inoculation with EF and FeNPs (0.5 mg L−1), resulting in significant increases of 14.7% and 82.12%, respectively, compared to the control. A decrease in irrigation level led to an increase in the percentage of essential oil in thyme plants while decreasing dominant essential oil compounds, thymol, and carvacrol. The levels of thymol and carvacrol in the essential oil were not affected by the interaction effects of drought stress, endophytes, and FeNPs. EF and FeNPs (1.5 mg L−1), respectively, caused significant increases of 17.44% and 29.87% compared to the control in the amount of thymol and significant increases of 13.75% and 31.01% in the amount of carvacrol. All FeNPs concentrations decreased the concentrations of ferulic acid, FeNPs (1.5 mg L−1) and PPO; these particles act as abiotic stressors at sub-toxic levels and become phytotoxic at higher concentrations.ConclusionsIt seems that combining endophytes with FeNPs showed promise in enhancing drought tolerance in T. vulgaris. These treatments have significantly contributed to the production of enzymatic antioxidants, flavonoids, and phenolic compounds, leading to a reduction in the amounts of oxidants. At the same time, they have improved both the quality and quantity of essential oils. This highlights the importance of establishing an effective antioxidant system in response to environmental stresses.Graphical

Pengaruh Penambahan Minyak Nabati pada Media Tumbuh terhadap Pertumbuhan Cendawan Entomopatogen Lecanicillium lecanii

Pest attacks are one of the challenges frequently encountered in rice cultivation. Effective and environmentally friendly control can be achieved with the entomopathogenic fungus Lecanicillium lecanii. The addition of vegetable oils such as peanut oil and castor oil can enhance the growth and development of the entomopathogenic fungus. This study aims to find the corn growth medium with the addition of vegetable oils that provides the best effect for the growth of the entomopathogenic fungus L. lecanii. The method used is a Completely Randomized Design (CRD) with a single factor consisting of 7 treatments with 4 replications: Control (Corn Medium); T1 (Corn + Peanut Oil 2 ml); T2 (Corn + Peanut Oil 5 ml); T3 (Corn + Peanut Oil 10 ml); J1 (Corn + Castor Oil 2 ml); J2 (Corn + Castor Oil 5 ml); J3 (Corn + Castor Oil 10 ml). The results of this study indicate that the addition of vegetable oils to the corn growth medium has a significant effect on the growth of the entomopathogenic fungus L. lecanii. The most effective growth medium for L. lecanii is corn medium + 10 ml peanut oil, providing an incubation period of 1 days and a colony diameter of 8.00 cm.

Uji Efektivitas Beberapa Media Tumbuh Alternatif Limbah Organik dengan Penambahan Tepung Kulit Udang terhadap Pertumbuhan Cendawan Entomopatogen Lecanicillium lecanii

Alternative control uses the entomopathogenic fungus Lecanicillium lecanii which is propagated using agro-industrial waste as a substrate for the production of locally available conidia. Propagation of L. lecanii was carried out on media supplemented with shrimp shell flour as additional nutrition. This research aims to provide the best alternative media for the growth of L. lecanii. The research was carried out at the Biological Agents Laboratory (BBPOPT), from March to May 2024. The method used was an experiment with a single factor Completely Randomized Design (CRD). This research consisted of 7 treatments in 4 repetitions: K (Corn Media); A (Sugar Bagasse); B (Tea Dregs); C (Sawdust); D (Sugar Bagasse + Shrimp Shell Flour); E (Tea Dregs + Shrimp Shell Flour); F (Sawdust + Shrimp Shell Flour). The results showed that L. Lecanii has a short incubation period, namely one hsi and alternative organic waste media with the addition of shrimp shell flour is more effectively used in the propagation of the L. lecanii fungus and has a significant effect on colony diameter (8.84 cm) in sawdust + shrimp shell flour. Alternative media with the addition of shrimp shell flour is recommended for the growth of the fungus L. lecanii.

Potential of California Variety Papaya Leaf Extract (Carica papaya L. California variety) As Bioherbicide of Snake Grass Weed (Cyperus rotundus L.)

Efforts to increase production yields are by controlling weeds. Weeds that often grow and we encounter in cultivated plants are weed grass (C. rotundus). California papaya leaves have the potential to be a bioherbicide because they contain allelochemicals that can inhibit weed growth. This study aims to determine the effect of papaya leaves (C. papaya) on the growth of teki grass (C. rotundus) and determinetheconcentration level of papaya leaf allelopathy that most effectively inhibits the growth of teki grass.The method used was a completely randomized design (CRD) with five replications and four treatmentsof0%, 30%, 50%, and 70%. For data analysis in this study using the SPSS version 25.0 program. Papaya leaf extract (C. papaya) is able to inhibit the growth of weed grass (C. rotundus). The concentration of papaya leaf extract (C. papaya) that most effectively inhibits the growth of weed is 50% concentration with a mortality rate (64%), for the lowest plant heightis50% concentration which is 13.84 cm, for the slowestgrowthrate is 50% concentrationwith a growthrate of -0.16 cm.

Combined Effect of Arbuscular Mycorrhizal Fungi and Bradyrhizobium japonicum on Growth, Yield and Nodulation in Soybean

Response of soybean for combined application of AM fungi and Bradyrhizobium japonicum were studied in pot culture experiment in completely randomized design (CRD). Dual inoculation of AM fungi and B. japonicum improved growth, yield, AMF colonization and nodulation in soybean plant, in comparison to single inoculation. Increase in the level of AMF inoculation promoted the growth significantly. Dry weight of the plant was 5.54 g/ plant and 6.02 g/ plant in plants treated with B. japonicum and AMF respectively, which significantly increased to 7.36 g/ plant up on combined application. Similar results were obtained in plant height, No. of pods /plant, seed index, nodulation, AMF density and colonization and chlamydospore population in soybean rhizosphere. Thus, the experiment signifies the importance of combined application for the improvement of crop growth and yield.

Physical Characteristics, Moisture Content and Organoleptic Properties of Afkir Laying Hen Meat with Different Marination Durations in Pineapple Crown Extract

Afkir laying hen meat has a tough texture, making it less desirable to consumers. Pineapple crown extract is known to improve the texture of spent laying hen meat due to its bromelain enzyme content. This study aims to determine the effect of marination duration using pineapple crown extract on the pH, cooking loss, water content, water holding capacity, texture, and organoleptic value of spent laying hen meat. The study was conducted using a Completely Randomized Design with 4 treatments (different marination times of 0, 2, 4, and 6 hours) and 5 replications. The parameters observed in this study included pH, cooking loss, water content, water holding capacity, texture, and organoleptic values. Test data were analyzed statistically using Analysis of Variance with a 5% significance level, and if significant effects were found, the analysis was followed by Duncan’s Multiple Range Test. Organoleptic test data were analyzed with the Kruskal-Wallis method, and if there were significant effects, further analysis was conducted using the Mann-Whitney test. The results showed a significant effect (P<0.05) of marination time using pineapple crown extract on the pH, water content, and organoleptic parameters, including taste, texture, color, and overall preference, with no significant effect (P>0.05) on cooking loss, water holding capacity, or the aroma attribute in the organoleptic test. Marination time also decreased the toughness of afkir laying hen meat texture, as analyzed descriptively. Marination for 2-6 hours decreased pH and water content, tenderized the texture, and improved meat preference. Keywords: Afkir laying hen; bromelain; tenderness; pineapple crown; marination

Effect of Palm Oil Mill Effluent (Pome) Concentration and Soil Type on the Growth of Oil Palm (Elaeis guinensis Jacq) in Pre-Nursery

The place of research was carried out at Educational and Research Garden of the STIPER Agricultural Institute which is located in Maguwoharjo Village, Depok District, Sleman Regency, Yogyakarta, Indonesia. The research location is located 118 meters above sea level. This research was conducted between March to June 2024. Two components make up the factorial experiment used in the Complete Randomized Design (CRD). The soil type had three levels: A1 = latosol soil, A2 = regusol soil, and A3 = grumusol soil, was the first factor. Palm Oil Mill Effluent (POME) concentration was the second factor. It was consisted into four levels: X1 was the control (NPK fertilizer), X2 was 150 ml per polybag, X3 was 300 ml per polybag, and X4 was 450 ml per polybag. So there were 12 treatment combinations (3 x 4 = 12) with 4 repetitions, so all the experiment 48 plants. Analysis of variance (ANOVA) was applied at the significant level of 5% to check the observation data. Duncan's Multiple Range Test (DMRT) was then used at a noticeable 5% level if there was significant difference. The weight parameters of fresh roots and the amount of chlorophyll in the leaves are influenced by the interaction of the soil and POME concentration. The composition, regusol soil and POME 450 ml/polybag provide an optimal combination of treatments. The type of soil has an impact on the weight of fresh and dry crowns, the number of leaves, the chlorophyll content in the leaves, the volume of roots, and the height of the plant. The best type of soil is regusol soil. Plant height, root volume, fresh and dry crown weight, and leaf chlorophyll content are all affected by POME concentration. The concentration of POME 450 ml/polybag showed the best results.

Performance Pheretima sp. and The Quality of Vermicompost on Media Using Clam and Snail Shell Flour

The abundance of green mussel shells, clam shells, and snail shells that have not been maximally utilized has caused environmental disturbances. These shells can be used as a substitute for worm food because of their good nutritional content. This research aimed to analyze the productivity of Pheretima sp. with the combination of shell powder (green mussel, clam, and snail) and cow dung to their living media (feed) and the quality of vermicompost. The data were analyzed using SAS Studio application with completely randomized design (CRD), 10 treatment levels, with 3 replications each. The observed variables included earthworm productivity, vermicompost quality, and vermicompost performance. The results of the studyshow a significant (P<0.05) on the use of green mussel shell 10% in all earthworm productivity variables, and show a significant (P<0.05) on the use of green mussel shell 30% in vermicompost quality and vermicompost performance. The combination of 10% green mussel shell powder in cow dung media can increase the productivity of Pheretima sp. The best vermicompost testing result on water spinach plants was found in the treatment with the addition of 30% green mussel shell powder in cow dung media.

The Productivity of Lumbricus rubellus Earthworms in Cow Manure Media with the Addition of Cricket Manure

Earthworms are one of the animals used as indicators to assess soil quality and fertility. In earthworm cultivation, several factors affect the life of earthworms, including temperature, pH, moisture, nutrient content, and media texture. This study aims to evaluate the productivity of earthworms (Lumbricus rubellus) in a mixed medium of cow manure and cricket manure. The method used in this study is a Completely Randomized Design (CRD) with 6 treatments and 3 replications, along with a Tukey posthoc test. The results showed that the addition of cricket manure to cow manure as a cultivation medium could increase earthworm productivity. The use of 90% cow manure with the addition of 10% cricket manure resulted in an average weight increase of 17 g, an average of 94 cocoons, and an average media reduction of 260.50 g. The addition of more than 10% cricket manure to cow manure could reduce earthworm productivity.

Biological Parameters and Feeding Potential of Micromus timidus Hagen (Neuroptera: Hemerobiidae) on Selected Aphid Species

In the present study, we investigated on the developmental biology, adult longevity and fecundity of Micromus timidus Hagen on Aphis craccivora (Koch), Aphis gossypii Glover, Rhopalosiphum maidis (Fitch) and Uroleucon compositae (Theobald) at room temperature (28±20C) and relative humidity of 70%. The study design followed was completely randomized design (CRD). The study was conducted with three replications with 10 larvae in each replication for biology studies and for adult parameters 3 replications with 10 pairs in each replication used. Shortest incubation period, II instar and III instar duration, total larval period, pre-pupal period, and egg to adult period of M. timidus were recorded on A. craccivora, shortest I instar duration was recorded on U. compositae and least pupal period on A. gossypii. The developmental periods for all trait were extended when fed on R. maidis. The reproductive traits viz., oviposition period and adult longevity (male and female) were maximum when M. timidus adults fed on A. gossypii, and the highest fecundity was recorded when fed on A. craccivora. Feeding potential in M. timidus increases progressively across successive instars, with III instar larvae exhibiting the highest aphid predation rate, and first instar larvae displaying the lowest feeding activity. The maximum feeding potential of the larva was noted on A. craccivora (142.47 ± 31.66), while, the adult feeding potential increased when it was given with A. gossypii (1335.10 ± 192.74).

Nano Chelated Iron and Boron Effect on Their Rates of Absorption and Transport by Moringa Plant

An experiment was carried out under a plastic house during 2019/2020 in Al-hindiya Herticaullare Station, Kerbala Province, Iraq. Seeds of Moringa Were sown in black polyethylene bags 5 kg soil capacity. Factorial experiment within Completely Randomized Design (C.R.D) with three replicates was adopted. The aim was to assess the effect of three Levels of the nano chelated Fe i.e. 0 ,180 and 360 mg. L-1, and Four Levels of B i.e. 0, 25 ,50 and 75 mg. L-1 spraying either once or twice – on the Fe and B rates of absorption and transport.

Growth, Carcass and Feed Consumption of Native Chicken Given Feed Containing Fermented Cocoa Husk Flour

The improvement of the performance of native chicken needs to be balanced with the improvement of its ration. The ingredients of the ration used do not need to be expensive, but good quality, easy to obtain, and available throughout the year. One of the feed ingredients that is widely available and is waste is Cocoa skin. Fermented Cocoa skin has a nutritional content of 2.63%, crude fat 20.14%, crude fiber, 10.39% crude protein, calcium 7.18%, and phosphorus 2.06%. Cocoa skin, in addition to its high crude fiber, also contains antinutrients in the form of tannins. Therefore, fermentation is necessary. The design used in this study was a Completely Randomized Design (CRD) with 5 (five) treatments and 3 (three) replications. The variables observed were Growth, ration consumption, protein consumption, Whole carcasses harvested when the chickens were 10 weeks old. The provision of fermented Cocoa skin up to 10% did not give a significant effect (P>0.05) on weight gain, final body weight, and whole carcass weight of native chicken aged 3-10 weeks. However, the provision of fermented Cocoa skin flour gave a significant effect on ration consumption, protein consumption, and energy consumption in native chicken aged 3-10 weeks. The best provision was obtained in the treatment of provision of fermented Cocoa skin flour as much as 5%

Utilization of Maggot Larvae (Hermetia illucens) for Processing Food Waste at the UIN Raden Intan Lampung Canteen

Waste management using maggot larvae (Hermetia illucens) can be an innovative strategy for reducing organic waste. This study aims to determine the effectiveness of maggot larvae in processing organic waste at the UIN Raden Intan Lampung Canteen. The research used a Completely Randomized Design (CRD) technique with 3 treatments and 3 replications. The observed parameters were duration, residue, and maggot weight. The organic waste used included vegetables, fruits, rice, and bones. The results showed that for bone and rice waste, maggots were able to reduce waste by 80%, producing an average residue of 141.3 grams with a decomposition duration of 515 minutes and a final maggot weight of 320.67 grams. For fruit and vegetable waste, maggots reduced waste by 58%, producing an average residue of 293 grams with a decomposition duration of 395 minutes and a final maggot weight of 560.33 grams. For mixed waste, maggots reduced waste by 81% with a decomposition duration of 496.6 minutes and a final maggot weight of 430.3 grams.

Growth performance, serum metabolites, plasma protein fraction, meat lipid profile and antioxidant activities of breast meat of broiler chicken fed diets containing Cinnamomum ceylon powder, Zingiber officinale powder and Moringa oleifera leafmeal supplem

An experiment was conducted to determine growth performance, serum metabolites, plasma protein fraction, meat lipid profile and antioxidant activities of meat of broilers as affected by Cinnamomium ceylon, Zingiber officinale and Moringa oleifera supplementation. A total of 112 day old chicks were allotted into four treatments replicated four times with seven birds per replicate in a completely randomized design (CRD). Basal diets were formulated for broiler starters (0-28days) and finisher phase (29-56) days. The basal diets were divided into 4 diets : diet 1- control diet without supplementation; diet 2- contained 0.2% Cinnamomum ceylon supplementation (CCS); diet 3- contained 0.2% Zingiber officinale supplementation (ZOS), diet 4-contained 0.2% Moringa oleifera leafmeal (MOLM) supplementation. The experiment lasted for 56 days. Results showed an improvement in final liveweight, weight gain, feed intake and feed conversion ratio of birds on 0.2% MOLM. Birds on phytogenic feed additives have lower values of urea, cholesterol, glucose, high density lipoprotein and low density lipoprotein. Birds administered phytogenic supplements exhibited much greater plasma albumin levels. Low levels of meat cholesterol, triglycerides and low density lipoprotein were found in birds fed phytogenic feed additives while high levels of high density lipoprotein were recorded for birds on phytogenic feed additives. The supplementation of broilers diets with phytogenic feed additives increased the glutathione peroxidase and superoxide dismutase and decreased lipid peroxidation. It was concluded that supplementation of broiler diets with Moringa oleifera leaf meal enhanced body weight gain, antioxidant activities also reduced cholesterol level in both serum metabolites and meat.

Effect of Treating Alfalfa Seeds with Gibberellin and Nano-fertilizer on Productive Characteristics and Protein Content in Hydroponic Cultures

This study was carried out in an experiment in an incubator designed for hydroponic seed germination with tight thermal insulation in the laboratories of the Department of Plant Production Technologies at the Technical College of Agriculture in Mosul, which is affiliated with the Northern Technical University in Iraq, where the seeds of the Dutch variety alfalfa (Medicago sativa. L) were germinated commercially and four times between 2022 and 2024. The experiment was carried out in a completely randomized design (CRD) with two factors and three replicates in perforated dishes with dimensions of 28 cm in length and 19 cm in width (area 532 cm2) at a seed rate of kg / m2, resulting in an average seed weight of 53 g in one dish. The first experimental factor was the germination periods, while the second factor was soaking the seeds in solutions of varying amounts made from gibberellin and nano-compound fertilizer with the comparison treatment, which is soaking in water alone, as follows: Gibberellin 100 ppm and given the symbol GA 100ppm- Gibberellin 200 ppm and given the symbol 200ppm GA- --Nano fertilizer 20:20:20 NPK at a concentration of 2000 ppm and given the symbol NPK2000ppm. -Nano fertilizer 20:20:20 NPK at a concentration of 4000 ppm and given the symbol NPK4000ppm. This study showed that the Gibberellin 200ppm GA treatment was significantly superior to the rest of the other soaking treatments in the characteristics of the height of the incubator (3.66 cm), as well as the wet weight of both seedlings and roots and their dry weight, which were (497.88, 56.97g/dish-1), respectively, The fresh and dried root weights were considerably greater in the same treatment, at (400.86 and 43.86 g/dish-1), respectively. while the comparison treatment was superior. Morally, it was significantly superior to the rest of the other soaking treatments in the characteristic of protein in the medium was (18.29%). As for the times of hydroponic seed germination, there were no significant differences between the times in all the studied characteristics, which enhances the high degree of incubator isolation, the accuracy of implementing the experiment, and the accuracy of taking the results.

Haematological response of starter broiler chickens fed diets containing graded levels of dried watermelon peel meal

The experiment was carried out to assess the hematological parameters of starter broiler chickens fed feed formulated with dried watermelon peel meal (DWPM). Ninety-six 21-day old broiler chickens were randomly assigned into four (4) treatments of twenty-four (24) birds each containing 0, 10, 20 and 30% DWPM and coded as D1, D2, D3 and D4 respectively in a completely randomized design (CRD). Each treatment groups were further sub-divided into four (4) sub-groups of eight (8) birds to serve as replicate. At the end of the experiment, one bird per replicate was selected for blood analysis. Packed cell volume (PCV), haemoglobin (Hb), white blood cells (WBC), red blood cells (RBC), mean corpuscular volume (MCV), platelets concentration (PC), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC), granulocyte and lymphocyte were measured. Results showed that the DWPM diet had no effect (P>0.05) on PCV, Hb, WBC, RBC, MCV, MCH, PC and MCHC. All mean values for the haematological parameters are above the normal range except WBC and RBC. In conclusion, the lack of significant effect suggests that, DWPM can be safely incorporated up to 12.5% in to the diets of starter broiler chicks without adverse haematological consequences. Nevertheless, careful consideration should be given to ensuring optimal nutritional balance and health status in broiler flocks.

The Effect of Adhesive Ratio on the Quality of Charcoal Briquettes

This study used cassava stem charcoal and charcoal from forest fires mixed with tapioca adhesive at different ratios, the aim was to analyze the effect of different adhesive ratios on the quality of charcoal briquettes.Charcoal briquettes are formed using a 40 bar press machine with a pressing time of 10 minutes. The mixture of charcoal briquette raw materials uses a tapioca adhesive ratio of 2%, 4%, 6%, 8% and 10%.Testing is carried out to determine the quality, then analyzed and compared with the SNI 01-6235-2000 standard.The effect of adhesive ratio on adhesive quality was analyzed using a Completely Randomized Design (CRD). The test results show the following values:density 0.54 g/cm3, moisture content 4.97%, compressive strength 28.43 kg/cm2, volatile matter content 36.02%, ash content 6.36%, fixed carbon content 56.45% and calorific value 6,195 cal/gobtained from a 10% adhesive ratio. The adhesive ratio has a very significant effect on the water content, compressive strength and calorific value; has a significant effect on the density value; and has no significant effect on the volatile matter value, ash content and bound carbon content.The results of charcoal briquette testing mostly meet the standards according to SNI (No. 01-6235-2000)

Enumeration of Genetic Variability Parameters and Association among Early Seedling Growth Parameters under Polyethylene Glycol (PEG) induced Osmotic Stress in rabi Sorghum (Sorghum bicolor (l.) Moench)

Aims: The research aims to explore the variability within diverse rabi sorghum germplasms and identify the relationships among different early seedling growth traits. This will provide valuable insights for future breeding programs, aiding in the development of resilient sorghum varieties that can contribute to improved food security and agricultural sustainability. Study Design: The study was conducted by employing factorial complete randomized design (FCRD) with two replications. Where, two factors was considered, genotype as first factor and PEG concentration as second. Place and Duration of Study: The experiment was conducted during rabi season of 2023 at University of Agricultural Sciences Dharwad, Karnataka. Methodology: A total of 156 germplasm lines of rabi sorghum were assessed in vitro for early seedling growth characteristics, including germination percentage, shoot length, root length, seedling dry weight, and seedling vigor indices I and II, under both control and osmotic stress conditions induced by polyethylene glycol (PEG). Osmotic stress was induced by dissolving PEG 6000 in distilled water to achieve the desired concentration. The germplasm lines were then subjected to germination and early growth under these conditions using the paper towel method. The genetic variability parameters for each trait were quantified to examine the degree of variability in the material used, and correlation analysis was performed to understand the relationships among the traits studied. Results: The variance analysis showed highly significant differences among genotypes, treatments, and the genotype-by-environment (G×E) interactions. Genetic variability parameters revealed considerable variability across all traits studied, which tended to increase with higher stress levels. Moderate to high GCV and PCV was observed for all the traits studied. Correlation analysis demonstrated a strong, significant positive relationship among all seedling parameters, except between root length and shoot length, as well as between shoot length and germination percentage under control conditions. Conclusion: The study emphasizes the effect of osmotic stress on the early vigor and growth of sorghum seedlings. The findings reveal significant variability in seedling traits, suggesting that selection can be employed for genetic enhancement of these traits. Correlation analysis identified specific traits or combinations of traits that should be considered when selecting genotypes tolerant to osmotic stress conditions. These findings offer great potential to guide and improve selection and breeding programs focused on enhancing drought tolerant sorghum varieties.