Complete Section Research Articles

Neutron transfer in 7Li+205Tl system

Neutron transfer cross sections for 7Li+205Tl system were measured near Coulomb barrier energies using online γ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\gamma $$\\end{document}-ray detection technique. One neutron stripping, two neutron stripping, and one neutron pickup cross sections have been extracted and are compared with the Coupled Reaction Channel (CRC) calculations. The systematics of one and two neutron stripping and pickup cross sections with a 7Li projectile on several targets show an approximate universal behaviour. A comparison of integrated neutron transfer cross sections with complete and incomplete fusion cross sections available with 7Li projectile is presented to understand the systematic behaviour. The neutron transfer along with cumulative sum of complete and incomplete fusion was found to explain the estimated reaction cross section in 7Li+205Tl system.

Research on intelligent cutting control technology of transverse moving machine for large cross-section roadheader

With the continuous development of the coal mine industry in our country, the cross-section of the roadway is getting larger and larger. The widely used EBZ series roadheader cannot complete the roadway cutting operation with a large cross-section at one time, so it usually needs to move the equipment left and right to complete all its cutting tasks. This paper studies the intelligent control technology of large cross-section roadway cutting, which mainly solves the positioning and attitude determination technology of roadheader in the large cross-section, and offers an accurate shape-cutting control technology and a path planning technology using a lateral moving machine. This paper proposes to combine the signals of laser sensor and fiber-optic inertial navigation system to form a combined positioning and attitude determination system, so as to make use of the advantages and avoid the disadvantages of their respective systems, and improve the accuracy of roadheader positioning and attitude determination. A swing kinematics model of the cutting arm is established, based on which the precise motion control of the first roadway section cutting and the second roadway section cutting can be realized. A key parameter calculation model of roadheader lateral moving machine is put forward to calculate the operation parameters for roadheader lateral moving machine path planning. Finally, the experimental research on intelligent cutting control technology of large cross-section roadheader is carried out in 12307 intelligent heading face of Wangjialing Coal Mine. The results show that the positioning error of the combined positioning and attitude determination system proposed in this paper is between 50-90 mm in the X-axis direction and between 25-45 mm in the Y-axis direction. The average attitude measurement error of heading angle is between 0.5 and 1.5°. This makes the technology appropriate for engineering use. The studied cutting control system achieves a cutting error of 25-50 mm for the first section, and a cutting error of 40-90 mm for the complete section. The complete section cutting error meets the ±100 mm cutting error requirement required in engineering. The intelligent cutting control technology of the lateral moving machine of the large-section tunnel boring machine studied in this paper can realize accurate cutting of the large-section tunnel of 5.6 meters. The research results of this paper provide a reference for the intelligent construction of heading faces.

Analytical railway line capacity methods from graph-theoretical and polyhedral perspectives

ABSTRACT This paper rigorously addresses the consumed capacity underestimation caused by division-based methods in graph theory and polyhedral geometry. Graph-theoretically, such underestimation occurs because subgraphs in shorter sections approximate their combined graph. Our polyhedral study suggests that the capacity analysis is computationally ‘easy’ because its linear programme in the complete section has an orthogonal projection on the Euclidean plane containing five proper faces, one being the objective-defined one-dimensional optimal facet. Geometrically, we conclude that the polyhedra of the linear programmes in shorter and complete sections have parallel facets, with the former nested within the latter. Consequently, the optimal values of the former cannot exceed those of the latter. With these theoretical insights, we derive a train operation labelling algorithm that takes proven linear time. Beyond enabling faster capacity computation, the findings deepen our understanding of the connection between infrastructure capacity utilisation and timetable structure, ultimately leading to more efficient railway optimisation.

Ultrastructural analysis of whole glomeruli using array tomography.

The renal glomerulus produces primary urine from blood plasma by ultrafiltration. The ultrastructure of the glomerulus is closely related to filtration function and disease development. The ultrastructure of glomeruli has mainly been evaluated using transmission electron microscopy; however, the volume that can be observed using transmission electron microscopy is extremely limited relative to the total volume of the glomerulus. Consequently, observing structures that exist in only one location in each glomerulus, such as the vascular pole, and evaluating low-density or localized lesions are challenging tasks. Array tomography (AT) is a technique used to analyze the ultrastructure of tissues and cells via scanning electron microscopy of serial sections. In this study, we present an AT workflow that is optimized for observing complete serial sections of the whole glomerulus, and we share several analytical examples that use the optimized AT workflow, demonstrating the usefulness of this approach. Overall, this AT workflow can be a powerful tool for structural and pathological evaluation of the glomerulus. This workflow is also expected to provide new insights into the ultrastructure of the glomerulus and its constituent cells.

Outcome Prediction by Diffusion Tensor Imaging (DTI) in Patients with Traumatic Injuries of the Median Nerve.

Background/Objectives: The accurate quantification of peripheral nerve axonal regeneration after injury is critically important. Current strategies are limited to detecting early reinnervation. DTI is an MRI modality permitting the assessment of fractional anisotropy, which increases with axonal regeneration. The aim of this pilot study is to evaluate DTI as a potential predictive factor of clinical outcome after median nerve section and microsurgical repair. Methods: We included 10 patients with a complete section of the median nerve, who underwent microsurgical repair up to 7 days after injury. The follow-up period was 1 year, including the current strategy with clinical visits, the Rosén-Lundborg score and electroneuromyography. Additionally, DTI MRI of the injured wrist was planned 1, 3 and 12 months post-operatively and once for the contralateral wrist. Results: The interobserver reliability of DTI measures was almost perfect (ICC 0.802). We report an early statistically significant increase in the fractional anisotropy value after median nerve repair, especially in the region located distal to the suture. Meanwhile, Rosén-Lundborg score gradually increased between the third and sixth month, and continued to increase between the sixth and twelfth month. Conclusions: DTI outcomes three months post-operation could offer greater predictability compared to current strategies. This would enable faster decision-making regarding the need for a potential re-operation in cases of inadequate early reinnervation.

Use of Melinex film for flat embedding tissue sections in LR White.

Tissue slices can undergo distortions during processing into resin for light and electron microscopy as a result of differential shrinkage of the various tissue components, and this may necessitate removal of a considerable amount of material from the final resin-embedded tissue block to ensure production of complete sections of the sample. To mitigate this problem, a number of techniques have been devised that ensure the sample is held flat during the final curing/polymerisation of the resin. For embedding in acrylic resins, oxygen must be excluded as it inhibits polymerisation, and methods devised for epoxy resin embedding are generally unsuitable. The method describes the preparation and use of air-tight flat-embedding chambers prepared from Melinex film and provides an inexpensive, technically simpler, and versatile alternative to chambers formed from either Thermanox coverslips or Aclar films that have previously been advocated for such purposes. Lay description: Tissue slices can undergo distortions during processing into resin for light and electron microscopy as a result of differential shrinkage of the various tissue components. Such distortions may necessitate removal of a considerable amount of material to ensure production of complete sections of the sample. For embedding in acrylic resins, oxygen must be excluded as it inhibits polymerisation, and methods devised for epoxy resin flat-embedding are generally unsuitable. Air-tight flat-embedding chambers prepared from either Thermanox coverslips, or a combination of PTFE-coated glass slides, polycarbonate film gaskets, and Aclar film have been advocated for such purposes. Thermanox coverslips are expensive and limited in size to 22mm × 60mm, and the alternative method is technically complicated. Melinex film is commercially available as 210mm × 297mm sheets and is approximately 1/20th the price of Thermanox and less than half the price of Aclar film. The method describes the preparation and use of embedding chambers made from Melinex film, glass slides and double-sided adhesive tape as a technically simpler, inexpensive and versatile alternative to both Thermanox coverslips and the Aclar film method.

Rapid method for paraffin embedding of precision-cut liver slices

Precision-cut liver slices (PCLS) are tissue explants extensively used as an ex vivo model for metabolism and toxicity studies. When in vitro assays are conducted, it is imperative to perform a histomorphological evaluation as part of the viability analyses throughout the assay time. It is considered that good quality PCLS histological sections are difficult to obtain because they are hard to manipulate, and may shrink or fold during processing. Moreover, bibliography is not detailed on the embedding processes used. In this article, we propose an adjusted and rapid method for paraffin embedding of PCLS from crossbreed steers. Each PCLS was covered with a piece of gauze and placed into a histological cassette. These cassettes were submitted to a series of baths: 80 % ethanol for 10 min; 3 baths of 96 % ethanol for 10 min; 3 baths of butanol for 10 min; 1 bath of butanol-paraffin (1:1) for 20 min in a 60 °C laboratory oven; and 3 baths of paraffin for 20 min in a 60 °C laboratory oven. Folded paper boxes were used to produce paraffin blocks. It was possible to obtain complete sections with preserved cell morphology and no artifacts, and tissue appearance was similar to previous PCLS processed through the routine protocol, demonstrating the adequacy of the method implemented.

Dating the middle and late Quaternary Caspian Sea-level fluctuations: First luminescence data from the coast of Turkmenistan

The Caspian region provides the basis of the stratigraphy and palaeogeography of the Pleistocene of Central Eurasia, and has been actively studied for more than 200 years. Many issues concerning the palaeogeographic history of the various basins of Caspian Quaternary remain unsolved, but the main problem centres on uncertainties in the chronology. Previous chronological studies have focused on a detailed description of the sedimentation history in the Lower Volga and Eastern Caucasus, ignoring a number of unique sites on the eastern Caspian coast, mainly due to inaccessibility. We obtained access to one of the most complete sections, western Cheleken, located on the Caspian coast of Turkmenistan. Within this sandy and generally lowland coast, the western part of the Cheleken peninsula forms a 10 km wide cliff opening a unique series of Middle Pleistocene - Holocene sediments which record in detail the history of Caspian transgressions and regressions. Stratigraphical, geomorphological and palaeontological studies of the section were supplemented with luminescence and radiocarbon dating. Analysis of Caspian molluscs and luminescence ages allowed the reconstruction of the sedimentary history of this region during one of the most important events of the Caspian Basin: the Early and Late Khazarian, Khvalynian and Novocaspian transgression/regression series. Luminescence dating of quartz and feldspar provided 12 OSL, IR50 and pIRIR290 ages, comparison of which showed that quartz grains were sufficiently bleached before deposition. Our chronology provides, for the first time, a deeper understanding of the sedimentation and environmental history in this region and the entire Caspian Sea. The Early Khazarian transgression occurred here at ∼220–200 ka (MIS 7). The Late Khazarian stage recorded in shallow-marine clays had relatively stable level ∼150–130 ka ago. In the second half of MIS 5 sea-level decreased and sedimentation was controlled by a large river, most probably the palaeo-Amu-Daria. The Early Khvalynian stage started at ∼27 ka and continued presumably until ∼14 ka.

Torque and Drag Analysis for Horizontal Extended Reach Coiled Tubing Drilling

One of the major elements faced in downhole drilling operations of oil and gas wells is the limited reach of coiled tubing (CT) in horizontal wellbores. To address this issue, this work creates an extended reach limit simulation of CT with starting bending curvature in a horizontal wellbore for an Iraqi well in the Ahdeb oil field. Using Drillbench software, a calculation approach is provided based on contact force and buckling models to determine the extended reach limit of CT. The findings of this study have significant implications for improving downhole drilling engineering design parameters and predicting the extended reach limit of CT. By considering factors such as friction resistance and contact force between the wellbore, a more accurate assessment of the CT’s abilities can be suggested based on well trajectory curvature and well devotion. To achieve this, a slim open hole with a diameter of 6 inches was drilled to a measured depth ranging from 3755 to 3986.5 m. The results showed that a consistent trend in azimuth and inclination in the completed section, with the hook load increasing by about 25 tons during pickup and decreasing by 11.4 tons during slack-off along the curved section due to friction effect. Additionally, elongation and relative stress showed slight increase during pickup in curved areas due to friction and temperature effect, while values decreased during slack-off due to drag action. Pressure loss in the curved section was found to be lower compared to the vertical section, primarily due to the bit nozzle discharge effect. Eventually, the friction coefficient values remained within acceptable industry limits. Ultimately, the study determined limited extended lengths for CT in this horizontal well.

Fusion and one-neutron stripping process for 6Li + 94Zr system around the Coulomb Barrier* *This research is supported by the National Nature Science Foundation of China (U2167204, 11975040, 1832130, 12375130), the Director's Foundation of Department of Nuclear Physics, China Institute of Atomic Energy 12SZJJ-202305. The Brazilian authors are grateful to the partial support from financial agencies FAPERJ, CNPq, and INTC-FNA

The complete and incomplete fusion cross section as well as one-neutron stripping process of 6Li + 94Zr system were measured at the energies around the Coulomb barrier by online γ-ray method. In addition to a 30% suppression factor when compared with the measured total fusion process, the complete fusion cross section in 6Li + 94Zr system was observed to be significantly lower than those in the nearby 6Li + 90, 96Zr system. The new experimental result implies that the coupling with breakup channel in the 6Li-induced fusion processes can be affected by the inner structure of the target, which is still not clear in any available model calculation. For the one-neutron stripping process, the direct production cross sections for each level in 95Zr were extracted and compared with the coupled reaction channel calculation, offering a unique opportunity to examine the single-particle nature of the produced excited states. Given the fact that an overall overestimation of the production cross section for 954-keV and 1618-keV levels was observed in the comparison, further investigation is highly demanded in order to understand the full reaction mechanism for the one-neutron stripping process induced by 6Li.

The Rafael gas-condensate discovery – a potential game-changer in the Canning Basin

The Rafael 1 exploration well discovered an abnormally high-pressured gas-condensate pool in the Kimberley region in late 2021. Data supports a minimum gross gas column of 165 m at the well location trapped within a large faulted four-way closure in a dolomite reservoir sequence with strong similarities and age equivalent to the oil-bearing Ungani Dolomite reservoir in the Ungani Field. A successful production test in March 2022 resulted in sustained natural flows of gas and condensate to surface. The presence of thick sandstones of the Anderson Formation and a complete Laurel section beneath the Meda Unconformity were unexpected based on the pre-drill interpretation. Serendipitously, it provided the perfect geological setting for a significant hydrocarbon accumulation and emphasised the need to consider multiple geological scenarios when evaluating prospects to capture the full range of uncertainties and potential pool sizes. Although the range in size of the contingent resources is wide and requires further drilling for refinement, the Rafael 1 discovery could be one of the most significant onshore gas discoveries since Waitsia 1 and provides impetus and incentive for continued exploration in the basin. Several pathways for commercialisation have been identified linked to the resource size. A comprehensive appraisal program is underway including the recently completed acquisition of a 200 km2 3D seismic survey to optimally locate wells, and plans for drilling to reduce uncertainty and support the first phase of development based on a low case contingent resource that could underpin the energy needs of the Kimberley region.

Further investigation on the fusion of 6Li with 209Bi target at near-barrier energies

Abstract The complete and incomplete fusion cross sections for 6Li+209Bi were measured via the in-beam γ-ray method around the Coulomb barrier. The cross sections of (deuteron captured) incomplete fusion (ICF) products were re-quantified experimentally for this reaction system. The results reveal that the ICF cross section is equivalent to that of complete fusion (CF) above the Coulomb barrier and dominant near or below the barrier. A theoretical calculation based on the Continuum Discretized Coupled Channel (CDCC) method is performed for the aforementioned CF and ICF cross sections, and the result is consistent with the experimental ones. The universal fusion function (UFF) is also compared with the measured CF cross section with different barrier parameters, demonstrating that the CF suppression factor is quite sensitive to the choice of potential, which can reflect both dynamic and static effects of breakup on the fusion process.

Semi-inclusive production of spin- 3/2 hadrons in deep inelastic scattering

We investigate the production of spin-3/2 hadrons in semi-inclusive deep inelastic lepton-nucleon scatterings. The complete differential cross section is derived through the kinematic analysis and expressed in terms of 288 structure functions, corresponding to all polarization configurations and azimuthal modulations. For an unpolarized lepton beam, half of the 192 structure functions have nonzero leading order contributions in the parton model, among which 42 are from rank-3 tensor polarized fragmentation functions of the hadron. For a polarized lepton beam, one third of the 96 structure functions contribute at the leading order and 14 of them are from rank-3 tensor polarized fragmentation functions. In addition to the formalism, we perform a model estimation of the spin transfer to a ShLLL polarized hadron and sizable asymmetry is expected. Therefore, these newly defined observables for the production of a spin-3/2 hadron in a deep inelastic scattering process can be explored in future experiments to understand nucleon spin structures and spin-dependent fragmentation functions. Published by the American Physical Society 2024

Reliability of Percutaneous Achilles Tenotomy in the Treatment of Idiopathic Clubfoot.

Talipesequinovarus is a congenital ankle/foot malformation that commonly affects newborns. In its treatment using the Ponseti method, an Achilles tenotomy is frequently needed to correct residual equinus deformity. Percutaneous (PC) tenotomy is the most commonly used technique and needs to be thoroughly evaluated. The question we needed to answer was: "Does PC Achilles tenotomy result in complete tendon sectioning"? This clinical study included 56 idiopathic clubfeet in 36 patients who presented during the first 6 months of life and were treated with Ponseti manipulation and casting followed by PC Achilles tenotomy. PC tenotomy was done under general anesthesia, and Thompson's calf squeeze test was performed intraoperatively just after tenotomy. In case of a negative test (ie, ankle plantar flexion with calf squeeze), the percutaneous incision was extended to assess the cause of the negative test. Forty-nine feet (87.5%) had a complete tenotomy, as evidenced by a positive Thompson's calf squeeze test. While 7 feet (12.5%) showed a negative test, and therefore the percutaneous incision was extended and all were found to have an incompletely divided Achilles tendon. The tendons were then completely divided, after which Thompson's test became positive in all. Percutaneous Achilles tenotomy is a reliable procedure, but was found to result in incomplete tendon sectioning in 12.5% of cases, affecting the range of ankle dorsiflexion. Thompson's squeeze test is reliable in picking up cases of incomplete tenotomy after the PC technique, and in these cases the incision should be extended for complete sectioning. Level I.

Investigation of reaction and α production cross sections with 9Be projectile

In order to investigate the contribution of α production in the reaction cross sections, measurements of elastic scattering and inclusive α particle angular distributions have been carried out with the 9Be projectile on 89Y, 124Sn, 159Tb, 198Pt, and 209Bi targets over a wide angular range at energies near the Coulomb barrier. The measured elastic scattering angular distributions were fitted with optical model calculations, and reaction cross sections were extracted. The same data were also analyzed using both global optical model potentials (Global OMP) and microscopic São Paulo potentials (SPP), to obtain the reaction cross sections. The data available in the literature for 9Be projectile includes the elastic scattering angular distributions, α production cross sections, and complete fusion cross sections on these and other targets at several energies are also utilized for comparative studies. The reaction cross section extracted from the three potentials (Best Fit, Global OMP and SPP) are in reasonable agreement for all the targets except for the energies below the barrier where the results from SPP deviate by 30-50%. Inclusive α particle production cross sections were also extracted by integrating the α particle angular distributions. The present data and data available from literature of reaction and α-particle production cross sections were utilized to make systematic studies. Systematics of reaction and α-particle production cross sections revealed their universal behavior. The ratio of complete fusion (CF), inclusive α cross sections, and their sum (CF+α) to reaction cross sections show that at sub-barrier energies inclusive α dominates and above barrier CF dominates, and cumulative of these two processes almost explains the reaction cross section at all energies over a wide range of target mass. A comparative study of α production with other weakly bound projectiles is also performed and a clear distinction between projectile types is observed, which is found to correlate well with α separation energies of the projectiles.

Flanagan's condensed protocol for neurodegenerative diseases. Implementation in a clinical autopsy setting with partial supervision of a neuropathologist.

The Condensed Protocol (CP) was originally developed for the evaluation of Alzheimer's Disease (AD) and other neurodegenerative diseases as a workable alternative to the complex and costly established autopsy guidelines. The study objective is to examine the degree of implementation of the CP in the pathology department of a third level university hospital in a period of 5years. Clinical autopsies performed between 2016 and 2021 on patients aged 65years or over and did not require a specific neuropathological examination were reviewed. Histological screening and staging of neurodegenerative diseases was performed using the original immunohistochemical stains. Out of 255 autopsies, 204 met the inclusion criteria and 190 could be reviewed. The CP was applied to 99 cases; histological signs of neurodegenerative disease were observed in 92. Sampling errors were detected in 59 cases. Immunohistochemical studies were performed in 68 cases. The diseases identified were: 31 cases of AD (12 low grade; 19 intermediate), 18 amyloid angiopathy, 15 primary age-related tauopathy, 6 argyrophilic grain disease, 3 progressive supranuclear palsy, 1 Lewy body disease (of 22 cases), and 2 limbic-predominant age TDP43 encephalopathy (of 5 cases). In 30 out of 83 cases, there was more severe vascular pathology in complete sections of frontal cortex and lentiform nucleus. The CP allows reliable detection and staging of AD and related neurodegenerative diseases in clinical autopsies. However, supervision by a neuropathologist seems necessary for a fully successful implementation of the CP in a clinical hospital setting.

Upper Ordovician–Lower Silurian deposits in the Zakola section (Ilych River, Northern Urals): Composition, structure, formation environments

Research subject. Section of boundary deposits, exposed in the right side of the Zakola stream (the right tributary of the Ilych River, Northern Urals) with a total thickness of 98 m, presented with rock samples (more than 100 pieces), thin sections (100 pieces), slabs (10 pieces), and chemical analyses (15 pieces). Aim. Detailed description of the most complete section of the Upper Ordovician–Lower Silurian boundary sediments of the Shantym facies complex with a stratigraphic reference of layers, lithological typification of sediments, and restoration of sedimentation conditions. Methods. Conventional lithological and paleontological methods were used to dissect the section. Sampling was carried out after 1 m; however, samples were additionally taken when detailed observations were possible to identify lithological and facial differences. The thin sections were studied by the classic method of optical microscopy. The study of the fauna complex was carried out both by collecting samples of the author’s rocks and by the materials of A.I. Antoshkina. In addition, stock materials were attracted. Results. In the section, 14 packs of rocks were identified, which are represented by 6 lithological types of secondary dolomites. Their distribution along the section, along with the presence of characteristic faunal remains, made it possible to establish three members in the section, which can be correlated with the Katian, Hirnantian, and Ruddanian stages of the General Stratigraphic Scale. Conclusion. The Katian deposits are represented by the Yaptikshor Formation (Strata 1), which was formed under the conditions of the lower sublittoral of a ramp-type carbonate platform with a transgressive carbonate tract and a diverse fauna. The beginning of the Hirnantian (Strata 2) is characterized by shallow water conditions of the upper sublittoral up to the littoral, which reflects a sharp shallowing at the Katian–Hirnantian boundary and corresponds to the beginning of the global early Hirnantian regression. The rocks exposed higher up the section indicate sedimentation with a certain rise in the sea level; however, the correlation of this part of the section with the upper part of the Verkh Ruchey Formation is complicated by the fragmentary nature of the outcrops. The overlying Ruddanian deposits (Strata 3) were accumulated on the outskirts of a ramp-type carbonate platform under the conditions of the lower sublittoral, which corresponds to the beginning of the Early Silurian global transgression.

Differential Immunoexpression of Inhibitory Immune Checkpoint Molecules and Clinicopathological Correlates in Keratoacanthoma, Primary Cutaneous Squamous Cell Carcinoma and Metastases.

Beyond established anti-programmed cell death protein 1/programmed cell death ligand 1 immunotherapy, T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibition motif domain (TIGIT) and its ligand CD155 are promising novel inhibitory immune checkpoint targets in human malignancies. Yet, in cutaneous squamous cell carcinoma, evidence on the collective expression patterns of these inhibitory immune checkpoints is scarce. Complete tumour sections of 36 cutaneous squamous cell carcinoma, 5 cutaneous metastases and 9 keratoacanthomas, a highly-differentiated, squamoproliferative tumour, with disparately benign biologic behaviour, were evaluated by immunohistochemistry for expression of programmed cell death ligand 1 (Tumor Proportion Score, Immune Cell Score), TIGIT, CD155 and CD8+ immune infiltrates. Unlike keratoacanthomas, cutaneous squamous cell carcinoma displayed a strong positive correlation of programmed cell death ligand 1 Tumor Proportion Score and CD115 expression (p < 0.001) with significantly higher programmed cell death ligand 1 Tumor Proportion Score (p < 0.001) and CD155 expression (p < 0.01) in poorly differentiated G3-cutaneous squamous cell carcinoma compared with keratoacanthomas. TIGIT+ infiltrates were significantly increased in programmed cell death ligand 1 Immune Cell Score positive primary tumours (p = 0.05). Yet, a strong positive correlation of TIGIT expression with CD8+ infiltrates was only detected in cutaneous squamous cell carcinoma (p < 0.01), but not keratoacanthomas. Providing a comprehensive overview on the collective landscape of inhibitory immune checkpoint expression, this study reveals associations of novel inhibitory immune checkpoint with CD8+ immune infiltrates and tumour differentiation and highlights the TIGIT/CD155 axis as a potential new target for cutaneous squamous cell carcinoma immunotherapy.

Heterogeneous microstructure development in additive friction-stir deposited Al-Mg-Si alloy

Aluminum alloy 6061-T6511 was printed by additive friction stir deposition onto a cast aluminum A206-T4 plate. Metallography, X-ray powder diffraction, X-ray pole figure analysis, and electron backscatter diffraction of the center, advancing side, and retreating side of the 6061 deposit reveals different texture and grain structure characteristics across the transverse direction and through each layer. These distinct microstructural domains are indicative of spatially varied temperature, strain rate, and strain accumulation during deposition, and consequently of regions differing in the fraction of dynamic recovery and dynamic recrystallization during deposition. Hardness mapping over the complete cross section at 0.75 mm resolution shows the alloy continues to age after active deposition, and hardness increases over the 5 mm nearest the final printed layer. Additional mapping at 0.10 mm resolution shows intralayer hardness variations of the deposit on the retreating side. These observations demonstrate that complex and non-uniform thermal-mechanical transients occur during additive friction stir deposition, resulting in spatially non-uniform microstructure and properties.

Can the General Public Be a Proxy for an "At-Risk" Group in a Patient Preference Study? A Disease Prevention Example in Rheumatoid Arthritis.

When selecting samples for patient preference studies, it may be difficult or impractical to recruit participants who are eligible for a particular treatment decision. However, a general public sample may not be an appropriate proxy. This study compares preferences for rheumatoid arthritis (RA) preventive treatments between members of the general public and first-degree relatives (FDRs) of confirmed RA patients to assess whether a sample of the general public can be used as a proxy for FDRs. Participants were asked to imagine they were experiencing arthralgia and had screening tests indicating a 60% chance of developing RA within 2 yrs. Using a discrete choice experiment, participants were offered a series of choices between no treatment and 2 unlabeled hypothetical treatments to reduce the risk of RA. To assess data quality, time to complete survey sections and comprehension questions were assessed. A random parameter logit model was used to obtain attribute-level estimates, which were used to calculate relative importance, maximum acceptable risk (MAR), and market shares of hypothetical preventive treatments. The FDR sample (n = 298) spent more time completing the survey and performed better on comprehension questions compared with the general public sample (n = 982). The relative importance ranking was similar between the general public and FDR participant samples; however, other relative preference measures involving weights including MARs and market share differed between groups, with FDRs having numerically higher MARs. In the context of RA prevention, the general public (average risk) may be a reasonable proxy for a more at-risk sample (FDRs) for overall relative importance ranking but not weights. The rationale for a proxy sample should be clearly justified. Participants from the general public were compared to first-degree relatives on their preferences for rheumatoid arthritis (RA) preventive treatments using a discrete choice experiment.Preferences were similar between groups in terms of the most important and least important attributes of preventive treatments, with effectiveness being the most important attribute. However, relative weights differed.Attention to the survey and predicted market shares of hypothetical RA preventive treatments differed between the general public and first-degree relatives.The general public may be a reasonable proxy for an at-risk group for patient preferences ranks but not weights in the disease prevention context; however, care should be taken in sample selection for patient preference studies when choosing nonpatients.