Complete Identity Research Articles

Methods for determining the toxicity of technogenic materials used in road construction

A method for determining the toxicity of soil and technogenic materials used in road construction has been developed. It is proposed to determine the toxicity of technogenic substances used as building materials for the construction of road surfaces by using biotesting on Colpoda steinii infusoria based on the test reaction of the survival rate of these infusoria. Soil samples for toxicity determination were taken at different distances from the roadbed (5, 10, 20, 25, 50 m from the edge of the road surface). During the experiment, it was found that anyone of the tested soil samples taken at a distance of 25 and 50 m from the roadway is not toxic to soil infusoria, and even after three hours of water extraction in all experimental samples, the test objects survived. These data on toxicity confirm additional experiments conducted by another method of biotesting on test cultures. In this case, soft spring wheat (Triticum vulgare L.) variety Novosibirsk 29 and watercress (Lepidium sativum) variety Donskoy were used as test crops. Experiments with these test cultures showed complete identity of the results obtained for toxicity according to the proposed method using Colpoda steinii infusoria.

VJ Segment Usage of TCR-Beta Repertoire in Monozygotic Cystic Fibrosis Twins.

Sixteen monozygotic cystic fibrosis (CF) twin pairs of whom 14 pairs were homozygous for the most common p.Phe508del CFTR mutation were selected from the European Cystic Fibrosis Twin and Sibling Study Cohort. The monozygotic twins were examined in their T cell receptor (TCR) repertoire in peripheral blood by amplicon sequencing of the CDR3 variable region of the ß-chain. The recruitment of TCR J and V genes for recombination and selection in the thymus showed a strong genetic influence in the CF twin cohort as indicated by the shortest Jensen-Shannon distance to the twin individual. Exceptions were the clinically most discordant and/or most severely affected twin pairs where clonal expansion probably caused by recurrent pulmonary infections overshadowed the impact of the identical genomic blueprint. In general the Simpson clonality was low indicating that the population of TCRß clonotypes of the CF twins was dominated by the naïve T-cell repertoire. Intrapair sharing of clonotypes was significantly more frequent among monozygotic CF twins than among pairs of unrelated CF patients. Complete nucleotide sequence identity was observed in about 0.11% of CDR3 sequences which partially should represent persisting fetal clones derived from the same progenitor T cells. Complete amino acid sequence identity was noted in 0.59% of clonotypes. Of the nearly 40,000 frequent amino acid clonotypes shared by at least two twin siblings 99.8% were already known within the immuneACCESS database and only 73 had yet not been detected indicating that the CDR3ß repertoire of CF children and adolescents does not carry a disease-specific signature but rather shares public clones with that of the non-CF community. Clonotypes shared within twin pairs and between unrelated CF siblings were highly abundant among healthy non-CF people, less represented in individuals with infectious disease and uncommon in patients with cancer. This subset of shared CF clonotypes defines CDR3 amino acid sequences that are more common in health than in disease.

A Questão do “Insílio” em Mulheres de Cinza: o Monolinguismo e a Monoidentidade do Outro

Focaliza-se o romance moçambicano como terreno propício para a representação do insílio, isto é, o exílio interno no âmbito da língua portuguesa, e paralelamente, dos processos de identificação cultural, a partir da representação máxima da personagem Imani, tradutora do português na comunidade local. Com base na leitura da obra O monolinguismo do outro ou a prótese de origem, de Derrida procura-se analisar a língua do colonizador enquanto instância surgida no intervalo do jogo entre a presença e a ausência, problematizando-a como o fora, isto é, “a língua que não é minha”, mas advinda do outro. À medida que escapa da total apreensão, esta língua traz consigo a promessa de transbordamento. Nessa linha de raciocínio, reafirma-se, de igual modo, a impossibilidade de uma identidade plena, pronta e acabada, demonstrando-se a configuração de identidades em constante deslocamento.

Розвиток бухгалтерського обліку в Україні на основі впровадження МСФЗ: проблеми і перспективи

For more than fifteen years in Ukraine, the process of reforming the accounting system has been carried out on the basis of the introduction of the IFRS methodology. New national standards have been adopted; many changes have been made to the Accounting Law that in the end gave enterprises the opportunity to apply original international standards, and their national counterparts. The purpose of the article is to systematize the problems of introducing IFRS into Ukrainian accounting practice and to substantiate the prospects for the development of domestic accounting on the basis of further implementation of the IFRS methodology. The problems of applying IFRS in Ukraine at different stages of their implementation were considered. The advantages and disadvantages of applying international standards in the accounting practice of Ukrainian enterprises were revealed. It was found that the employees of accounting services were not sufficiently prepared for the preparation of financial statements in accordance with IFRS, in connection with which the demand for accounting outsourcing services increased and there was a need to develop and conduct accounting training seminars. The adoption of national standards on the basis of international standards should only in theory ensure their complete identity, but in practice disagreements arose between the norms of certain standards. These circumstances led to the need for wider application of original IFRS, rather than their national counterparts. For national supervisory and regulatory bodies, IFRS have become an additional tool for effective monitoring and supervision of the economic activities of enterprises. The use of IFRS has opened access to Western capital markets for Ukrainian enterprises.

Plasmodium vivax metacaspase 1 (PvMCA1) catalytic domain is conserved in field isolates from Brazilian Amazon.

In the present study, we investigated the genetic diversity of Plasmodium vivax metacaspase 1 (PvMCA1) catalytic domain in two municipalities of the main malaria hotspot in Brazil, i.e., the Juruá Valley, and observed complete sequence identity among all P. vivax field isolates and the Sal-1 reference strain. Analysis of PvMCA1 catalytic domain in different P. vivax genomic sequences publicly available also revealed a high degree of conservation worldwide, with very few amino acid substitutions that were not related to putative histidine and cysteine catalytic residues, whose involvement with the active site of protease was herein predicted by molecular modeling. The genetic conservation presented by PvMCA1 may contribute to its eligibility as a druggable target candidate in vivax malaria.

Babesia gibsoni emerging with high prevalence and co-infections in “fighting dogs” in Hungary

Babesia gibsoni is considered as an emerging protozoan parasite of dogs in North America and Europe. However, no data have been published on its prevalence, molecular-phylogenetic characteristics and associated co-infections in dogs used for illegal fighting (i.e. predisposed to acquiring this piroplasm via biting) in Europe. In this study, blood samples from 79 American Staffordshire Terrier dogs, confiscated for illegal dog fights, were molecularly analyzed for tick-borne pathogens. Babesiagibsoni was detected in 32 dogs, i.e. with a prevalence of 40.5%. In addition, Babesia vulpes was found in 8 samples (prevalence of 10.1%), for the first time in dogs in Hungary. Canine hemoplasmas were also identified in 49 samples (62%): only Mycoplasma haemocanis in 32 (40.5%) dogs, only “Candidatus Mycoplasma haematoparvum” in 9 (11.4%) dogs, and both hemoplasmas in 8 (10.1%) dogs. Thus, hemoplasma infections also showed a particularly high prevalence in this dog population. Based on a partial fragment of the 18S rRNA gene, B. gibsoni from Hungary exhibited complete sequence identity with conspecific strains reported from Europe and Asia. The cytochrome c oxidase subunit 1 (cox1) gene sequence of this isolate showed the closest identity with B. gibsoni reported from Japan but had a nonsynonymous mutation (M33I). Furthermore, the 11 B. gibsoni-positive samples analyzed for sequence variants of the cytochrome b (cytb) gene showed the presence of a common mutation (P310S). Most importantly, B. gibsoni had two further nonsynonymous mutations, M121I and F258L, in a dog with severe and relapsing anemia following atovaquone treatment. Phylogenetically, both cytb sequence variants clustered together, with a clear geographical pattern showing the closest relationship of both haplotypes identified in Hungary with those from China and Japan. To the best of our knowledge, this is the first cox1 and cytb characterization of B. gibsoni in Europe, as well as the first report on the emergence of this piroplasm and hemoplasmas with high prevalence among “fighting dogs” north of the Mediterranean Basin.

Research on Identification Algorithm Based on ECG Signal and Improved Convolutional Neural Network

In the research of ECG signal identity recognition, most of them adopt the method of feature extraction and recognition model separation, extract the time domain features, transform domain features of the original signal, or combine the features with the cross domain. Then the model is used to complete the recognition and classification. In this paper, an advanced improved convolutional neural network model is proposed, which integrates feature extraction and classification to complete identity recognition. ECG data selected from the ecg-id database and MIT-BIH arrhythmia database are directly sent to the model for automatic sign extraction after hierarchical denoising with wavelet tools and then identified. This method achieves the highest recognition rate of 98.49% on ecg-id database and 99.35% on ECG data of MIT-BIH arrhythmia database. The high reliability of the algorithm and the universality of wireless sensors in mobile devices make this research has high commercial value.

Gaudapadacharya “asparsa yoga” for attaining “no mind”: A historical method of advaita vedanta for teaching “human liberation” in a profound way

This article is based on the two verses from the 3rd chapter known as “Advaita Prakaranam” (section of nonduality) of text called “Mandukya Karika” written by the author Gaudapada. At the beginning of the text Gaudapada tells, the problems which human beings have in the world (Samsara) are due to the perception of duality (subject-object duality). He says, the duality causes Samsara (problems in the worldly life) and nonduality (one without a second) is the freedom. Hence, “Advaita” (nonduality) is the freedom (Moksha) and duality (Dvaitam) is “Samsara” (Worldly troubles or bondage). According to Shankara's commentary on Gaudapada's texts, “no-mind” can be attained by constant practice of discrimination between the real and the unreal (repeated discrimination), all based upon reasoning. Gaudapada says “Amanibhava” (no-mind) means managing the mind or spiritualizing the mind. It means when a person realizes the truth about oneself as the “Existence, consciousness, Bliss” (The Absolute or Brahman) then, in the mind, there will be no more desiring or reaching out or grasping. When the mind finds nothing out there to grasp then it becomes a “nongrasping mind” (Agraham) that is called a “no-mind” state. This “no mind” state is referred to “freedom or liberation” from worldly suffering according to Advaita Vedanta Philosophy. This state of complete identity with nondual Brahman, arrived at as a result of discrimination and negation of phenomena, is the Vedantic conception of Samadhi (which is quite different from any mystical state described as Samadhi in the Yoga system).

Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells.

SummaryPrevious analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point “mutations” in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.

To the technique of painting Nissl`evsky bodies

In view of the growing interest presented by Nissl's bodies for researchers studying the physiological and pathological structure of nerve cells, I consider it useful to publish the coloring method I have developed. In addition to the simplicity of the technique and the demonstrativeness of the preparations obtained, the proposed method has the advantage of great constancy: to this I can add that the studies of the fresh nervous cells (my studies have not yet been published) reveal the complete identity of the pictures obtained in the volume and in other cases.

Peptide Binding to HLA-E Molecules in Humans, Nonhuman Primates, and Mice Reveals Unique Binding Peptides but Remarkably Conserved Anchor Residues.

Ag presentation via the nonclassical MHC class Ib molecule HLA-E, with nearly complete identity between the two alleles expressed in humans, HLA-E*01:01 and HLA-E*01:03, can lead to the activation of unconventional T cells in humans. Despite this virtual genetic monomorphism, differences in peptide repertoires binding to the two allelic variants have been reported. To further dissect and compare peptide binding to HLA-E*01:01 and HLA-E*01:03, we used an UV-mediated peptide exchange binding assay and an HPLC-based competition binding assay. In addition, we investigated binding of these same peptides to Mamu-E, the nonhuman primate homologue of human HLA-E, and to the HLA-E-like molecule Qa-1b in mice. We next exploited the differences and homologies in the peptide binding pockets of these four molecules to identify allele specific as well as common features of peptide binding motifs across species. Our results reveal differences in peptide binding preferences and intensities for each human HLA-E variant compared with Mamu-E and Qa-1b Using extended peptide libraries, we identified and refined the peptide binding motifs for each of the four molecules and found that they share main anchor positions, evidenced by conserved amino acid preferences across the four HLA-E molecules studied. In addition, we also identified differences in peptide binding motifs, which could explain the observed variations in peptide binding preferences and affinities for each of the four HLA-E-like molecules. Our results could help with guiding the selection of candidate pathogen-derived peptides with the capacity to target HLA-E-restricted T cells that could be mobilized in vaccination and immunotherapeutic strategies.

Типология ономасиологических моделей прилагательных-композитов русского языка

The subject matter of this article relates to the complex description of adjectives-composites of the Russian language within the onomasiological class “person’s social feature”. There have been analysed organization features of composites formation and ways of onomasiological structure definition as to composite adjectives within the onomasiological class. All adjective-composites of modern Russian are considered as univerbalisation composites, derivation composites and units of quasi-composition. Univerbalisation composites demonstrate a complete semantic identity with a producing word-combination. Derivation composites have shown the relations of external motivation between a basic word-combination and a new composite. Units of quasi-composition are formed on the basis of structural models of univerbalisation and derivation. The analysis of these linguistic units is carried out according to their belonging to a certain onomasiological model consisting of an onomasiological basis and an onomasiological feature that allows to distinguish 17 models of adjective composites. The author comes to the conclusion that the onomasiological class should be understood as a set of nominathems, in which the feature description is realized in one corresponding grammatical class of units (adjectives) with the help of the same structural method, mostly with the help of all kinds of composites. This research has developed the modern nomination theory and provides a basis for complex description of language units.

Compatible recurrent identities of the sandpile group and maximal stable configurations

In the abelian sandpile model, recurrent chip configurations are of interest as they are a natural choice of coset representatives under the quotient of the reduced Laplacian. We investigate graphs whose recurrent identities with respect to different sinks are compatible with each other. The maximal stable configuration is the simplest recurrent chip configuration, and graphs whose recurrent identities equal the maximal stable configuration are of particular interest, and are said to have the complete maximal identity property. We prove that given any graph G one can attach trees to the vertices of G to yield a graph with the complete maximal identity property. We conclude with several intriguing conjectures about the complete maximal identity property of various graph products.

UTERUS DUPLEX SEPARATUS S. DIDELPHYS

The congenital deformity of the uterus duplex separatus occurs, according to Schrder, so rarely that it was more often found on the section table next to such deformities that made the fetus unable to live, and only recently have a number of cases in adult women been published. In my six-year zemstvo practice on 87,000 men and women sick with all diseases in general, it comes across only the second time. Unfortunately, I cannot describe the first case, because the patient, left for a more detailed study, left without being written down even in the book; but both cases are so similar that the thought of the complete identity of the patients involuntarily appears.

A circular RNA map for human induced pluripotent stem cells of foetal origin.

BackgroundAdult skin fibroblasts represent the most common starting cell type used to generate human induced pluripotent stem cells (F-hiPSC) for clinical studies. Yet, a foetal source would offer unique advantages, primarily the absence of accumulated somatic mutations. Herein, we generated hiPSC from cord blood multipotent mesenchymal stromal cells (MSC-hiPSC) and compared them with F-hiPSC. Assessment of the full activation of the pluripotency gene regulatory network (PGRN) focused on circular RNA (circRNA), recently proposed to participate in the control of pluripotency.MethodsReprogramming was achieved by a footprint-free strategy. Self-renewal and pluripotency of cord blood MSC-hiPSC were investigated in vitro and in vivo, compared to parental MSC, to embryonic stem cells and to F-hiPSC. High-throughput array-based approaches and bioinformatics analyses were applied to address the PGRN.FindingsCord blood MSC-hiPSC successfully acquired a complete pluripotent identity. Functional comparison with F-hiPSC showed no differences in terms of i) generation of mesenchymal-like derivatives, ii) their subsequent adipogenic, osteogenic and chondrogenic commitment, and iii) their hematopoietic support ability. At the transcriptional level, specific subsets of mRNA, miRNA and circRNA (n = 4,429) were evidenced, casting a further layer of complexity on the PGRN regulatory crosstalk.InterpretationA circRNA map of transcripts associated to naïve and primed pluripotency is provided for hiPSC of clinical-grade foetal origin, offering insights on still unreported regulatory circuits of the PGRN to consider for the optimization and development of efficient differentiation protocols for clinical translation.FundingThis research was funded by Ricerca Corrente 2012–2018 by the Italian Ministry of Health.

BUDGET ASSESSMENT FOR INVESTMENT-CONSTRUCTION AND DEVELOPMENT PROJECTS (THEORY AND PRACTICE)

The practical value of standards in the field of project management is often discussed by specialists in various fields of project activity. Practicing project managers at the lower and middle levels often complain about the lack of clear and effective tools for solving specific problems of the project management execution. However, the skillful standards application may increase the success likelihood of a wide range of diverse projects with obtaining the expected business values “at the output” of the project. The systematic experience of the best specialists, acquired during the implementation of various projects and placed in the standards, can become the basis for the professional competencies development and the formation of competitive advantages for project companies in various industries. The aim of this study is to establish links between the methods recommended by the Project Management Body of Knowledge (PMBoK) for determining the budget of projects and the methods used in practice for determining the cost of construction projects, formalized in the current regulatory documents and state building codes of Ukraine. To this end, the PMBoK standard determined the basic methodological foundations of the project cost management process, its components and characteristic features. Then, based on recent publications and building standards, the main stages of the life cycle of construction projects were established, in which the cost of design work is assessed, the goals of such an assessment and the project participants that carry it out are determined. As a result, a complete methodological identity was established between the project cost estimation methods recommended by the PMBoK standard and the construction cost determination methods specified in the current regulatory documents of the Ukrainian construction industry, which confirms the full suitability of the PMBoK standard for use in construction project management practice. Research findings determine the use of the standard by both novice specialists and qualified project managers of construction projects.

Taphonomic methods and a database to establish the origin of sedimentary silicified rocks from the Middle-recent Gravettian open-air site of La Picardie (Indre-et-Loire, France)

For several years, multidisciplinary archaeological operations organized by the Ministry of Culture and named ‘Collective Research Projects’ (PCR) have enabled the design and use of a descriptive database for a part of southern and central France. This data base describes and stores the main geological aspects of sedimentary silicified rocks of chemical, biochemical or diagenetic origin (abbreviated SSR in the rest of the text) and their primary and secondary outcrops. Data were collected through a survey form describing the flint formations and using different observation grids which make up complete identity cards for each type of flint. Using their spatial attributes, these different data are linked to a shape file for flint formations, themselves digitized with ArcGIS and QGis on the basis of harmonized protocols with several layers of the WebMapService (WMS) Infoterre of the BRGM. Today, data sharing is made possible through the use of the ArcGisOnLine platform (AGOL) meeting the needs of the various users.Applied to an archaeological series, the database considers the concept of an evolutionary chain of SSR and extends an enquiry into the prehistoric relationship between SSR and their environments. According to the same criteria as those defined for the characterization of geological samples (petrology and alterology forms), it allows one to reconstruct the route traveled by any flint before its collection by prehistoric humans and then, by the use of a taphonomic form, decrypts the post-depositional SSR evolution in order to decipher the intensity and chronology of the mechanisms likely to have taken part in the sedimento-genesis of the archaeological site.In terms of understanding paleogeography, the ultimate aim of this approach, the accuracy of petro-archaeological studies is firmly established rather than being anecdotal. By demonstrating that only quantity, genetic and gitological diversity and the way in which materials from coherent geological spaces (i.e. geotopes) are introduced into the archaeological sites, allows one to discuss the method of acquisition of the materials concerned and thus to approach an understanding of the organization of prehistoric societies through their management of landscape and their relationship to territoriality. After the application of this method using the database the results are presented for particular materials coming from the Middle Gravettian series of la Picardie (Preuilly-sur-Claise, Indre-et-Loire, France).

Examining relaxivities in suspensions of nanodiamonds grafted by magnetic entities: comparison of two approaches.

Detonation nanodiamonds (DND) with Gd3+ ions directly grafted to the DND surface have recently demonstrated enhanced relaxivity for protons in aqueous suspensions. Herewith, the relaxivity measurements were done on a series of suspensions with the gadolinium content varied by changing number of Gd3+ ions grafted per each DND particle whereas the DND content in each suspension was kept the same. Such an approach to vary the contrast agent content differs from that commonly used in the relaxivity measurements. In the common approach, contrast agents are directly dissolved/suspended in media. Aiming to test validity of the unconventional approach, in the present study we follow the common way of measurement relaxivity: using variable concentrations of carriers (DND particles) in aqueous suspension keeping the number of Gd3+ ions per each carrier fixed. 1H NMR relaxation measurements of aqueous suspensions of DND with Gd3+ ions directly grafted to the DND surface were carried out at room temperature (293K or 20°C) in the external magnetic field B0 = 8.0T. Comparative study of two approaches for measuring relaxivity in suspensions containing DND as magnetic entities' carriers reveals complete identity of techniques in use.

IMPACT OF HLA-DPB1 MATCHING ON CLINICAL OUTCOMES AFTER HAPLOIDENTICAL-RELATED HEMATOPOIETIC STEM CELL TRANSPLANTATION.

The impact of HLA-DPB1 compatibility and its role as a transplantation antigen in haploidentical-related hematopoietic stem cell transplant (haplo-R-HSCT) have not been established, and a negative effect on survival has been suggested. The objective of the determine was to study the frequency and clinical effects of incompatibility at the HLA-DPB1 locus in the haplo-R-HSCT setting. Clinical records and electronic files of 91 patients with a hematological disease who underwent haplo-HSCT from January 2009 to October 2017 in a university medical center were scrutinized. Overall survival (OS) was estimated by the Kaplan-Meier method; the cumulative incidence of transplant-related mortality (TRM) and relapse rates was determined. Acute graft-versus-host disease was assessed by binary logistic regression. Cox regression model with a 95% confidence interval was used to examine the association between the different variables and their effect on OS. Of the 91 donor-recipient pairs, 24 (26.37%) shared complete DPB1 identity, 60 (65.93%) had a mismatch at one allele, and 7 (7.70%) were mismatched at two alleles. Twenty-four different HLA-DPB1 alleles were found; the most frequent were DPB1*04:01 (34.1%) and DPB1*04:02 (27.5%). Two-year OS, the cumulative incidence of TRM and relapse was 51.3 ± 6.8%, 28 ± 6% and 60 ± 7.8% for all haplo-related transplants, respectively, with no statistical difference between HLA-DPB1 matched and partially matched patients. In Cox regression analysis, no risk factors associated with OS, TRM, or relapses were identified. HLA-DPB1 mismatching in the haplo-R-HSCT setting did not influence transplant outcomes and was clinically tolerable. A high degree of homozygosity was found.

Rickettsia parkeri (Rickettsiales: Rickettsiaceae) in the Sky Islands of West Texas.

Rickettsia parkeri, a tick-borne pathogen distributed throughout several countries of the Americas, causes a mild to moderately severe, eschar-associated spotted fever rickettsiosis. Although most U.S. cases of R. parkeri rickettsiosis are reported from southeastern states, some have been reported recently from remote regions of southern Arizona. These cases are linked to R. parkeri-infected ticks of the Amblyomma maculatum (Acari: Ixodidae) group found in several isolated mountain ranges of southern Arizona and New Mexico, referred to as 'sky islands'. Archival records also document ticks of the A. maculatum group collected from domestic and wild animals in West Texas. We surveyed sites in two sky island chains of Jeff Davis and Brewster counties to document the off-host occurrence of these ticks and identify the presence of R. parkeri in the Trans-Pecos region of Texas. During August 2019, 43 adult A. maculatum group ticks were flagged from vegetation or removed from a road-killed, female mule deer. Of 39 samples evaluated by PCR, eight contained a partial sca0 sequence with complete identity to R. parkeri and two with complete identity to 'Candidatus Rickettsia andeanae', a species of undetermined pathogenicity. Four isolates of R. parkeri were obtained using cell culture. Persons at risk for R. parkeri rickettsiosis include those who work or recreate in these mountains, such as hikers, backpackers, research scientists, foresters, and border enforcement personnel. Additional investigations are needed to define the distribution of these medically important arthropods in other parts of the southwestern United States and northern Mexico.