This publication describes research on a possibility of controllable delivery of doxorubicin (DOX) into glioblastoma (GB) cells, being inside non-covalent construct with anti-EGFR DNA aptamer by intercalating into artificially created duplex. The construct has been made with previously described DNA aptamer GR20 (46 nucleotides), with 3’-end 18 nucleotides extension (GR20h), which was hybridized with the complementary DNA oligonucleotides (h). The duplex assembly is effective, the construct GR20hh is stable at 37 ºС, Tm = 59 ºС. DOX is intercalated into the construct. By applying xCelligence Real-Time Cell Analysis (RTCA) combined with self-created data processing, it has been shown that during a treatment of cell culture DOX, inside the non-covalent construct GR20hh – DOX, saves cytotoxic ability, though a kinetics of toxic action of the complex on GB cells is completely different from the kinetics of DOX along. The unique approach and the data are the bases for a development of both a regulation and a targeting of DOX cytotoxic activity toward specific GB cells.