Complement Fixation Test For Diagnosis Research Articles

Immune complex decomplementation of canine sera for use in a complement-fixation test for diagnosis of visceral leishmaniasis.

Canine sera frequently become anti-complementary when heat-inactivated at 56 degrees C for 30 min, and generally cannot be used in standard complement-fixation (CF) assays. Therefore, a procedure was developed for decomplementing canine sera by absorption with particulate immune complexes consisting of sheep erythrocyte stroma optimally sensitized with anti-sheep erythrocyte antibody (hemolysin). Canine sera incubated for 20 min at 30 degrees C with sensitized stroma consistently showed less than 10% residual complement and were not anti-complementary. This decomplementation procedure was applied in a complement-fixation (CF) test for detection of serum antibodies during canine visceral leishmaniasis. Two groups of German shepherd dogs were injected intravenously with Leishmania donovani or L. donovani chagasi amastigotes, and the course of infections was followed for 12 weeks. Using freeze-thaw sonicate preparations of L. donovani parasites as antigen, reciprocal CF antibody titers above 24 were detectable in sera 7 weeks after infection and gradually increased to a maximum titer of 775 at 12 weeks. Sera from control dogs had mean titers of 24. This improved methodology enhances the potential of the CF test in the serodiagnosis of canine leishmaniasis.

Adaptation of a complement fixation test for large scale serological diagnosis of bovine leptospirosis

Summary Details are given of a method of performing a semi-automated complement fixation test for the diagnosis of bovine leptospirosis. A “Dynatiter” machine is used which enables 288 sera to be tested per hour. In a comparative study it was shown that using this semi-automated test fewer than 5% of antibody titres differed from those obtained using a manual test, either with single antigens or a polyvalent antigen.

Comparison of rapid card agglutination test with the complement fixation test for diagnosis of Anaplasma marginale infection in Cattle in Colombia

Three hundred and thirty-nine serum samples collected from nine Holstein-Friesian calves before and during natural infection with Anaplasma marginale on the north coast of Colombia were used to compare the complement fixation (CF) and rapid card agglutination tests (CT) for the diagnosis of anaplasmosis. The positive agglutination of the CT always developed several days after the first CF reaction but then persisted. In contrast, the CF test showed an initial sharp rise in titer but then showed fluctuations between trace and 1 : 80 titers. The results indicated that under field conditions the CT was the simpler and more reliable test for the diagnosis of anaplasmosis in a single animal. The CF test remains useful in experimental situations where the earliest knowledge is required of the magnitude of immunological response to challenge.

Significance of the Complement-fixation Test in Diagnosis of Amoebiasis in an Endemic Area

Significance of the Complement-fixation Test in Diagnosis of Amoebiasis in an Endemic Area

Significance of the Complement-fixation Test in Diagnosis of Amoebiasis in an endemic area

Significance of the Complement-fixation Test in Diagnosis of Amoebiasis in an endemic area

Serologic Response of Chickens to Experimental Infection with Hemophilus gallinarum, and Their Immunity to Challenge

THE study of the immunity of chickens to infection with Hemophilus gallinarum has been hampered by the lack of serologic tests. Only recently Kato et al. (1962) reported on the use of agglutination and complement fixation tests for diagnosis of infection due to H. gallinarum, and Page (1962) reported briefly on the use of a plate agglutination test to classify isolates of H. gallinarum into antigenic groups.As early as 1934 De Blieck (1934) showed that chickens that recovered from an infection of H. gallinarum had a substantial degree of immunity. This observation was also confirmed by Schalm and Beach (1936). Page et al. (1963) inoculated chickens with a bacterin of H. gallinarum and determined their degree of immunity to challenge with a virulent H. gallinarum, but he did not follow their serologic reaction.In this paper we describe the serologic response of chickens to experimental infection with H. gallinarum,…

Direct complement-fixation test for diagnosis of ornithosis in turkeys.

SummaryEmploying a soluble antigen, complement-fixation reactions were obtained with turkey antiornithosis sera. Antigens consisting of viable allantoic and killed yolk-sac virus suspensions did not show the same degree of activity. The direct complement-fixation reaction was as sensitive as the indirect complement-fixation test; therefore, the use of the direct test for the study and diagnosis of turkey ornithosis was suggested.

Complement Fixation Tests for Diagnosis of Mumps Applied to Typical and Suspect Cases of Mumps Meningo‐Encephalitis.

Complement Fixation Tests for Diagnosis of Mumps Applied to Typical and Suspect Cases of Mumps Meningo‐Encephalitis.