Objective: The aim of this study was to investigate the relationship between musculoskeletal complaints, alexithymia defined as difficulty in the description and recognition of emotions, depression and quality of life in university students. Materials and Methods: 396 (233 female, 163 male) university students aged 18-25 were included. Extended Nordic Musculoskeletal Questionnaire was used to evaluate the musculoskeletal disorders, Toronto Alexithymia Scale was used to evaluate alexithymia, Beck Depression Inventory was used to evaluate the emotional state, Short Form-36 was used to evaluate the quality of life. Statistical analysis including descriptive and correlation was performed, p<0.05 was accepted for statistical significance. Results: Body regions which were most commonly reported for experiencing pain, low back (49.2%), neck (43.7%), upper back (40.2%) at some point in their lifetime, low back (38.2%), neck (35.4%), upper back (33.1%) during the past 12 months, low back (28.5%), neck (28.3%), upper back (24.5%) in the past month, low back (11.4%), neck (11.4%), upper back (10.1%) on the day of study. It was found that there was a correlation between the complaints of low back and neck pain with depression levels (p=0.00). Conclusion: In cases, musculoskeletal pain was the most common spine in all periods. This situation can be caused by the exposure of students to their fixed body positions in their daily lives for a long time. Although the quality of life of cases was high in terms of quality of life, we observed that emotionally affected negatively.