Cultivated land horizontal ecological compensation is an essential means of reconciling agricultural ecosystem protection and regional economic development. It is important to design a horizontal ecological compensation standard for cultivated land. Unfortunately, there are some defects in the existing quantitative assessments of horizontal cultivated land ecological compensation. In order to raise the accuracy of ecological compensation amounts, this study established an improved ecological footprint model based on the ecosystem service function, focused on estimating the value of ecosystem service function, ecological footprint, ecological carrying capacity, ecological balance index and ecological compensation values of cultivated land in all cities of Jiangxi province. It then analyzed the rationality of ecological compensation amounts in Jiangxi province, which is one of the 13 provinces of major grain-producing areas in China. The results show the following: (1) The total value of soil conservation service function, carbon sequestration and oxygen release service function and ecosystem service function in Jiangxi province showed a spatial distribution trend of "gradually increasing around Poyang Lake Basin". (2) The cultivated land ecological deficit areas in Jiangxi province are Nanchang City, Jiujiang City and Pingxiang City; ecological surplus areas are Yichun City, Ji'an City and eight other cities; and there is an obvious "Spatial Agglomeration" phenomenon in ecological deficit and ecological surplus areas where ecological deficit areas are mainly concentrated in the northwest region of Jiangxi. (3) The amount needed to attain fair ecological compensation for cultivated land is 5.2 times the payment amount for cultivated land; this indicated there is larger arable land, a favorable condition for agricultural cultivation, and better supply capacity of ecosystem services in most of the cities of Jiangxi. (4) The compensation amount for cultivated land ecological surplus areas in Jiangxi province is generally higher than the cost of ecological protection, and its proportion in GDP, fiscal revenue and agriculture-related expenditure is significantly higher than that in ecological deficit areas; this indicated that the compensation value of cultivated land could play the driving role in the protective behavior for cultivated land. The results provide a theoretical and methodological reference for the construction of horizontal ecological compensation standards for cultivated land.