Comparative Functional Analysis Research Articles

A comparative analysis of oropharyngeal functions in preterm and term children with cerebral palsy

This study aims to compare term and preterm children with Cerebral Palsy (CP) in terms of their oropharyngeal functions. A total of 50 children with CP participated in the study, and were divided into two groups according to their birth history including preterm group (gestational age below 37 weeks; 60 % female; mean age = 6.86 ± 4.35 years) and term group (gestational age between 37 and 41 weeks; 60 % female; mean age = 6.48 ± 4.86 years). Chewing and swallowing functions were evaluated in terms of oropharyngeal functions. Chewing evaluation was performed by using the Karaduman Chewing Performance Scale (KCPS) and the Turkish version of the Mastication Observation and Evaluation Instrument (T-MOE). The pediatric version of the Eating Assessment Tool (PEDI-EAT-10) was used to evaluate swallowing performance of children. In addition, the Behavioral Pediatric Feeding Assessment Scale (BPFAS) was used to assess feeding behaviors of children. There were significant differences between groups in terms of KCPS (p = 0.03), T-MOE (p = 0.01), and PEDI-EAT-10 scores (p = 0.04). There was a significant difference between groups in terms of the parental frequency score from the BPFAS (p = 0.04). Oropharyngeal functions of preterm children with CP were more adversely affected than term children with CP. Clinicians working with children with CP should be aware of the risks of preterm birth on oropharyngeal functions, and take precautions for oropharyngeal dysfunction in the early period in preterm children with CP.

Enhancing peak prediction in residential load forecasting with soft dynamic time wrapping loss functions

Short-term residential load forecasting plays a crucial role in smart grids, ensuring an optimal match between energy demands and generation. With the inherent volatility of residential load patterns, deep learning has gained attention due to its ability to capture complex nonlinear relationships within hidden layers. However, most existing studies have relied on default loss functions such as mean squared error (MSE) or mean absolute error (MAE) for neural networks. These loss functions, while effective in overall prediction accuracy, lack specialized focus on accurately predicting load peaks. This article presents a comparative analysis of soft-DTW loss function, a smoothed formulation of Dynamic Time Wrapping (DTW), compared to other commonly used loss functions, in order to assess its effectiveness in improving peak prediction accuracy. To evaluate peak performance, we introduce a novel evaluation methodology using confusion matrix and propose new errors for peak position and peak load, tailored specifically for assessing peak performance in short-term load forecasting. Our results demonstrate the superiority of soft-DTW in capturing and predicting load peaks, surpassing other commonly used loss functions. Furthermore, the combination of soft-DTW with other loss functions, such as soft-DTW + MSE, soft-DTW + MAE, and soft-DTW + TDI (Time Distortion Index), also enhances peak prediction. However, the differences between these combined soft-DTW loss functions are not substantial. These findings highlight the significance of utilizing specialized loss functions, like soft-DTW, to improve peak prediction accuracy in short-term load forecasting.

Assessment of Clavicular Shortening in Displaced Midshaft Clavicular Fractures Treated by Nonoperative Method, Plating, and Elastic Nailing: Comparative Functional Analysis\'A Case Series

Assessment of Clavicular Shortening in Displaced Midshaft Clavicular Fractures Treated by Nonoperative Method, Plating, and Elastic Nailing: Comparative Functional Analysis\'A Case Series

A comparative analysis of gastrointestinal tract barrier function and immune markers in gilt vs. sow progeny at birth and weaning.

Progeny born to primiparous sows (gilt progeny; GP) have lower birth, weaning and slaughter weights than sow progeny (SP). GP also have reduced gastrointestinal tract (GIT) development, as evidenced by lower organ weights. Therefore, the aim of this experiment was to quantify changes in GIT barrier function that occur in birth and weaning, representing two major challenges to the young piglet. The effects of parity (GP vs. SP) in GIT barrier integrity function were quantified at four timepoints: birth (~0h), 24h after birth (24h), 1-d preweaning (PrW), and 1-d postweaning (PoW) in commercially reared piglets. Due to inherent differences between newborn and weanling pigs, the results were analyzed in two cohorts, birth (0 vs. 24h, n = 31) and weaning (PrW vs. PoW, n = 40). Samples of the stomach, jejunum, ileum, and colon were excised after euthanasia and barrier integrity was quantified by measuring transepithelial resistance (TER), macromolecular permeability, the abundance of inflammatory proteins (IL-8, IL-1β, and TNF-α) and tight junction proteins (claudin-2 and -3). Papp was characterized using a dual tracer approach comprising 4 KDa fluorescein isothiocyanate (FD4) and 150kDa tetramethyl rhodamine isothiocyanate (T150)-labeled dextrans. Characteristic effects of the initiation of feeding and weaning were observed on the GIT with the initiation of feeding, such as increasing TER and reducing Papp at 24h, consistent with mucosal growth (P = 0.058) This was accompanied by increased cytokine abundance as evidenced by elevations in TNF-α and IL-1β. However, GP had increased IL-8 abundance (P = 0.011 and 0.063 for jejunum and ileum respectively) at birth than 24h overall. In the weaning cohort, jejunal and ileal permeability to FD4 was higher in GP (P = 0.05 and 0.022, respectively) while only higher ileal T150 was observed in GP (P = 0.032). Ileal claudin-2 abundance tended to be higher in SP overall (P = 0.063), but GP ileal claudin-2 expression was upregulated weaning while no change was observed in SP (P = 0.043). Finally, other than a higher jejunal TNF-α abundance observed in SP (P = 0.016), no other effect of parity was observed on inflammatory markers in the weaning cohort. The results from this study indicate that the GIT of GP have poorer adaptation to early life events, with the response to weaning, being more challenging which is likely to contribute to poorer postweaning growth.

In silico comparative structural and functional analysis of dengue virus (Serotypes 1 to 4) envelope protein

In silico comparative structural and functional analysis of dengue virus (Serotypes 1 to 4) envelope protein

Comparative functional analyses of PHR1, PHL1, and PHL4 transcription factors in regulating Arabidopsis responses to phosphate starvation.

To cope with phosphate (Pi) starvation, plants trigger an array of adaptive responses to sustain their growth and development. These responses are largely controlled at transcriptional levels. In Arabidopsis (Arabidopsis thaliana), PHOSPHATE RESPONSE 1 (PHR1) is a key regulator of plant physiological and transcriptional responses to Pi starvation. PHR1 belongs to a MYB-CC-type transcription factor family which contains 15 members. In this PHR1 family, PHR1/PHR1-like 1(PHL1) and PHL2/PHL3 form two distinct modules in regulating plant development and transcriptional responses to Pi starvation. PHL4 is the most closely related member to PHR1. Previously, using the phr1phl4 mutant, we showed that PHL4 is also involved in regulating plant Pi responses. However, the precise roles of PHL1 and PHL4 in regulating plant Pi responses and their functional relationships with PHR1 have not been clearly defined. In this work, we further used the phl1phl4 and phr1phl1phl4 mutants to perform comparative phenotypic and transcriptomic analyses with phr1, phr1phl1, and phr1phl4. The results showed that both PHL1 and PHL4 act redundantly and equally with PHR1 to regulate leaf senescence, Pi starvation induced-inhibition of primary root growth, and accumulation of anthocyanins in shoots. Unlike PHR1 and PHL1, however, the role of PHL4 in maintaining Pi homeostasis is negligible. In regulating transcriptional responses to Pi starvation at genomic levels, both PHL1 and PHL4 play minor roles when acts alone, however, they act synergistically with PHR1. In regulating Pi starvation-responsive genes, PHL4 also function less than PHL1 in terms of the number of the genes it regulates and the magnitude of gene transcription it affects. Furthermore, no synergistic interaction was found between PHL1 and PHL4 in regulating plant response to Pi starvation. Therefore, our results clarified the roles of PHL1 and PHL4 in regulating plant responses to Pi starvation. In addition, this work revealed a new function of these three transcription factors in regulating flowering time.

Exploring the Regulation and Function of Rpl3l in the Development of Early-Onset Dilated Cardiomyopathy and Congestive Heart Failure Using Systems Genetics Approach.

Cardiomyopathies, diseases affecting the myocardium, are common causes of congestive heart failure (CHF) and sudden cardiac death. Recently, biallelic variants in ribosomal protein L3-like (RPL3L) have been reported to be associated with severe neonatal dilated cardiomyopathy (DCM) and CHF. This study employs a systems genetics approach to gain understanding of the regulatory mechanisms underlying the role of RPL3L in DCM. Genetic correlation, expression quantitative trait loci (eQTL) mapping, differential expression analysis and comparative functional analysis were performed using cardiac gene expression data from the patients and murine genetic reference populations (GRPs) of BXD mice (recombinant inbred strains from a cross of C57BL/6J and DBA/2J mice). Additionally, immune infiltration analysis was performed to understand the relationship between DCM, immune cells and RPL3L expression. Systems genetics analysis identified high expression of Rpl3l mRNA, which ranged from 11.31 to 12.16 across murine GRPs of BXD mice, with an ~1.8-fold difference. Pathways such as "diabetic cardiomyopathy", "focal adhesion", "oxidative phosphorylation" and "DCM" were significantly associated with Rpl3l. eQTL mapping suggested Myl4 (Chr 11) and Sdha (Chr 13) as the upstream regulators of Rpl3l. The mRNA expression of Rpl3l, Myl4 and Sdha was significantly correlated with multiple echocardiography traits in BXD mice. Immune infiltration analysis revealed a significant association of RPL3L and SDHA with seven immune cells (CD4, CD8-naive T cell, CD8 T cell, macrophages, cytotoxic T cell, gamma delta T cell and exhausted T cell) that were also differentially infiltrated between heart samples obtained from DCM patients and normal individuals. RPL3L is highly expressed in the heart tissue of humans and mice. Expression of Rpl3l and its upstream regulators, Myl4 and Sdha, correlate with multiple cardiac function traits in murine GRPs of BXD mice, while RPL3L and SDHA correlate with immune cell infiltration in DCM patient hearts, suggesting important roles for RPL3L in DCM and CHF pathogenesis via immune inflammation, necessitating experimental validations of Myl4 and Sdha in Rpl3l regulation.

Raman study of the spin–lattice excitations of the layered antiferromagnets MPSe3 (M = Fe, Mn)

Magnetism in van der Waals (vdW) materials has recently garnered significant attention due to their potential for advanced applications. In this study, we investigate the magnetic transition in layered MPSe3 (M = Fe, Mn) bulk flakes utilizing temperature-dependent Raman spectroscopy. Our findings reveal unique features in the Raman spectrum that are strongly correlated with the antiferromagnetic ordering in MPSe3. We analyze the spin-dependent Raman scattering modes in MPSe3 by applying the Suzuki–Kamimura formalism. Consequently, our comparative analyses of the spin correlation function and the reduced magnetization provide compelling evidence of the 2D Ising characteristic especially in FePSe3. Our results clearly show the potential of MPSe3 as a prominent platform for exploring fundamental aspects of low-dimensional magnetism.

A comparative analysis of accident modification functions for traffic law enforcement

Traffic law enforcement is a road safety measure whose effects on accidents or injuries is best described by means of a function rather than a point estimate. An informative function should comprise both increases and decreases in enforcement. Currently available accident modification functions cannot serve this need. A fruitful approach to developing accident modification functions covering both increases and decreases in enforcement is differences-in-differences estimates based on multivariate accident prediction models. The paper explains how to develop such estimates and illustrates them. The interpretation of the results of empirical studies can be informed by a game-theoretic model of the effects of enforcement, previously published in Accident Analysis and Prevention (Bjørnskau and Elvik 1992, 507–520).

A comparative analysis of various activation functions and optimizers in a convolutional neural network for hyperspectral image classification

A comparative analysis of various activation functions and optimizers in a convolutional neural network for hyperspectral image classification

Attention deficit disorder with hyperactivity: comparative analysis of executive functions between genres based on neurofeedback training

Attention Deficit Hyperactivity Disorder (ADHD) is a neurobiological disorder that presents a characteristic pattern of inattention and/or hyperactivity-impulsivity. ADHD directly affects Executive Functions (EF), responsible for regulating social behavior and cognitive skills. In the clinical context, the symptoms can present themselves in unique ways in each patient. Therefore, we can use different approaches to evaluate these symptoms. This article aims to analyze, through the evaluation of executive functions performed through psychological tests and neurofeedback, how ADHD symptoms are presented in males and females. Psychological tests Digit Span, Corsi Cubes, Verbal Fluency and Five Digit Test (FDT) were used. In addition, a neurofeedback game that adapts the Stroop test in an interactive application was applied through a Brain-Computer Interface. A tool developed was tested with 21 children, 11 with a confirmed diagnosis of ADHD and to 10 without the presence of this disorder. Through statistical analysis, we come to establish promises. The results of the neurofeedback analysis met what is expected in the literature, which demonstrate that patients with ADHD have a greater number of slow waves and less beta waves in the prefrontal cortex or, which indicate low stimulation of the central nervous system.

Comparative genomic survey and functional analysis of DKKL1 during spermatogenesis in the Chinese soft-shelled turtle (Pelodiscus sinensis)

A feature of the Chinese soft-shelled turtle (Pelodiscus sinensis) is seasonal spermatogenesis; however, the underlying molecular mechanism is not well clarified. Here, we firstly cloned and characterized P. sinensis DKKL1, and then performed comparative genomic studies, expression analysis, and functional validation. P. sinensis DKKL1 had 2 putative N-glycosylation sites and 16 phosphorylation sites. DKKL1 also had classic transmembrane structures that were extracellularly localized. DKKL1's genetic distance was close to turtles, followed by amphibians and mammals, but its genetic distance was far from fishes. DKKL1 genes from different species shared distinct genomic characteristics. Meanwhile, they were also relatively conserved among themselves, at least from the perspective of classes. Notably, the transcription factors associated with spermatogenesis were also identified, containing CTCF, EWSR1, and FOXL2. DKKL1 exhibited sexually dimorphic expression only in adult gonads, which was significantly higher than that in other somatic tissues (P < 0.001), and was barely expressed in embryonic gonads. DKKL1 transcripts showed a strong signal in sperm, while faint signals were detected in other male germ cells. DKKL1 in adult testes progressively increased per month (P < 0.05), displaying a seasonal expression trait. DKKL1 was significantly downregulated in testes cells after the sex hormones (17β-estradiol and 17α-methyltestosterone) and Wnt/β-catenin inhibitor treatment (P < 0.05). Likewise, the Wnt/β-catenin inhibitor treatment dramatically repressed CTCF, EWSR1, and FOXL2 expression. Conversely, they were markedly upregulated after the 17β-estradiol and 17α-methyltestosterone treatment, suggesting that the three transcription factors might bind to different promoter regions, thereby negatively regulating DKKL1 transcription in response to the changes in the estrogen and androgen pathways, and positively controlling DKKL1 transcription in answer to the alterations in the Wnt/β-catenin pathway. Knockdown of DKKL1 significantly reduced the relative expression of HMGB2 and SPATS1 (P < 0.01), suggesting that it may be involved in seasonal spermatogenesis of P. sinensis through a positive regulatory interaction with these two genes. Overall, our findings provide novel insights into the genome evolution and potential functions of seasonal spermatogenesis of P. sinensis DKKL1.

Introducing the STReaC (Spike Train Response Classification) toolbox

Background:This work presents a toolbox that implements methodology for automated classification of diverse neural responses to optogenetic stimulation or other changes in conditions, based on spike train recordings. New Method:The toolbox implements what we call the Spike Train Response Classification algorithm (STReaC), which compares measurements of activity during a baseline period with analogous measurements during a subsequent period to identify various responses that might result from an event such as introduction of a sustained stimulus. The analyzed response types span a variety of patterns involving distinct time courses of increased firing, or excitation, decreased firing, or inhibition, or combinations of these. Excitation (inhibition) is identified from a comparative analysis of the spike density function (interspike interval function) for the baseline period relative to the corresponding function for the response period. Results:The STReaC algorithm as implemented in this toolbox provides a user-friendly, tunable, objective methodology that can detect a variety of neuronal response types and associated subtleties. We demonstrate this with single-unit neural recordings of rodent substantia nigra pars reticulata (SNr) during optogenetic stimulation of the globus pallidus externa (GPe). Comparison with existing methods:In several examples, we illustrate how the toolbox classifies responses in situations in which traditional methods (spike counting and visual inspection) either fail to detect a response or provide a false positive. Conclusions:The STReaC toolbox provides a simple, efficient approach for classifying spike trains into a variety of response types defined relative to a period of baseline spiking.

Gender Aspects of Cognitive Functioning in Former COVID-19 Patients

This paper presents a comparative analysis of cognitive functioning in women and men after COVID-19. A comprehensive assessment covered coping behavior and life purpose orientation in their connection with cognitive functions. It involved 65 former COVID-19 patients (26 men and 39 women). The general cognitive state was assessed with the help of the Mini-Mental State Examination (MMSE) Scale while the Frontal Assessment Batter (FAB) Test made it possible to determine the level of self-control in planning behavior. The coping behavior was evaluated using R. Lazarus’s Ways of Coping Questionnaire (WCQ). The semantic orientation of behavior was carried out using the Life Purpose Orientation technique developed by D. A. Leontiev. The research revealed some gender-related differences. The men demonstrated no connection between their coping behavior and the cognitive state. They had a lower sense of purpose and were mostly past-oriented. The women, on the contrary, showed a strong relationship between coping behavior, cognitive state, and life purpose orientation. In women, the restoration of cognitive functions led to a decrease in productive and unproductive behavioral responses to stressful situations, as well as to a greater sense of purpose and retrospective analysis.

Neonicotinoid insecticide imidacloprid induces chemosensory deficits in a nontarget parasitoid wasp

Chemical pesticides are widely used to manage the population of arthropod pests. Their increasing use in agriculture has raised concerns about their harmful effects on nontarget organisms, particularly some beneficial insects such as parasitoid wasps. To assess the potential risk and ecological safety of chemical pesticides, it is necessary to understand their impacts on the physiology and behaviour of those important natural enemies of arthropod pests. Here, we applied the Drosophila parasitoid Leptopilina drosophilae as a study model to investigate the effects of sublethal doses of imidacloprid, a widely used neonicotinoid insecticide. Our results demonstrated the detrimental effects of imidacloprid on the host-searching behaviour of L. drosophilae females and the courtship behaviour of L. drosophilae males. Comparative transcriptome and functional analysis provided further insights into the potential mechanisms underlying the impaired behaviours, with the downregulated expression of certain chemoreception genes in both female and male exposed wasps. Our findings thus emphasize the importance of understanding the risks associated with the use of chemical pesticides and the need to develop more eco-friendly pest management strategies for a sustainable balance between chemical and biological control.

Impact of industrial pollution on children's lungs: A comparative analysis of pulmonary function in critically polluted and non-polluted areas in western India

BackgroundAir pollution is a pressing global health concern, particularly in low- and middle-income countries like India. Children living near industrialized and critically polluted areas (CPAs) face heightened health risks due to their developing respiratory systems. This study aimed to assess the impact of industrial pollution on children's lung function in a CPA in western India. MethodologyThe study evaluated 362 children studying in 5th to 7th standard from both a CPA and a relatively non-polluted area (NPA) for comparison. Ambient and indoor air quality measurements were taken, and pulmonary function tests were conducted to assess lung health. Group difference and regression analysis were performed with SPSS 26.0. ResultsThe CPA group showed significantly higher air pollution levels and a higher prevalence of obstructive and restrictive patterns in pulmonary function compared to the NPA group. The findings also revealed that children residing in CPA exhibited a significant decline of 530 ml forced expiratory flow rate (FEF 25–75%), and a reduction of 3.9% FEV1/FVC ratio when compared to children residing in relatively NPA. ConclusionWhile this study's cross-sectional design has limitations, it provides valuable evidence of the impact of air pollution on children's lungs in a semi-urban industrialized area. To mitigate these health risks, integrating respiratory health check-ups, including pulmonary function tests, in existing health programs, like Rastriya Bal Swasthya Karyakram (RBSK), is recommended for early detection and intervention. Implementing initiatives such as enhancing green belts around schools and continuous air quality monitoring will be crucial.

Enhancing user experience and usability in engineering education: A comparative analysis of multiple functions and tangible user interfaces

A study was conducted at University Tenaga Nasional to compare engineering students’ preferences in using augmented reality technology user interface (UI) applications to solve selected engineering problems. The study utilized a cross-sectional design and the Wilcoxon signed-rank test approach within a controlled environment to analyse the difference in rankings of engineering applications. The study also measured learnability and assessed the software quality using questionnaire for user interaction satisfaction. The multiple function UI was found to be statistically significant more effective than the tangible user interface (TUI) for engineering students. The study provides reliable evidence that multiple function UI is a better option in engineering education than TUI.

Comparative Analysis of the Excitation Functions of 238U as Breeder Fuel Using OPTMAN Code.

Comparative Analysis of the Excitation Functions of 238U as Breeder Fuel Using OPTMAN Code.

Estimation of efficiency of the information exchange on digital networks of communication of terrestrial and air basing: algorithmic and the software

Article is devoted development algorithmic and the software for an estimation of information efficiency of digital networks of communication of a range meter and decimeter waves on existing means of relaying, and also digital networks of communication with usage of a network of retranslators for the unmanned aerial vehicles providing maintenance of demanded efficiency of an information exchange at changes of the input traffic and destabilizing actions. As a result of the led analysis the urgency of application of structurally steady network of relaying on the basis of unmanned aerial vehicles which provides formation of the spatially-distributed access point for terrestrial and air users, for the purpose of the decision of tasks of effective allocation of information highways and frictionless delivery of the information on considered digital networks of communication is formulated. Algorithms are developed for the proof of perspectivity of the given approach and models of estimation of efficiency of an information exchange on digital networks of communication on the basis of the generalized index, providing an adequate estimation in the conditions of change of the input traffic and influence of noises. The comparative analysis of functioning of considered digital networks of communication with usage of existing means of relaying and offered networks of retranslators on the basis of unmanned aerial vehicles is carried out. The main outputs by results of researches are formulated and practical recommendations for choice an optimal variant of creation of researched digital networks of communication are made. Perspectives of further usage of the received results are planned at the organization of complex multiple loop adaptation of digital networks of communication of high mobility with difficult and dynamic changing structures.

서킷 트레이닝을 통한 엘리트 수구 선수들의 신체 조성과 운동 체력, 심폐기능 및 혈중 젖산 농도 비교분석

서킷 트레이닝을 통한 엘리트 수구 선수들의 신체 조성과 운동 체력, 심폐기능 및 혈중 젖산 농도 비교분석