Comparative Analysis Of Biochemical Parameters Research Articles

Comparative Analysis of Biochemical Parameters, Thermal Behavior, Rheological Features, and Gelling Characteristics of Thai Ligor Hybrid Chicken and Broiler Meats

Genetic differences typically cause differences in the structure and function of proteins in meat. The objective of this research was to examine the biochemical characteristics and functional behavior of proteins in fresh composite meat from Thai Ligor hybrid chicken (LC) and commercial broiler chicken (BC). The composite meat samples, which comprise minced breast and thigh without skin from 20 chicken carcasses in a 1:1 (w/w) ratio, were randomly selected for analysis using the completely random design (CRD). Results showed that BC meat exhibited higher ultimate pH after 24 h, Ca2+-ATPase activity, and trichloroacetic acid (TCA)-soluble peptide content compared to LC meat (p < 0.05). While both meat types showed non-significant differences in reactive sulfhydryl (SH) levels (p > 0.05), LC meat exhibited higher hydrophobicity compared to BC meat (p < 0.05). Differential scanning calorimetry (DSC) analysis revealed a single transition peak in all samples. LC meat exhibited higher thermal stability than BC meat, with transition peaks at 91 °C and 81 °C, respectively, in non-sodium chloride (NaCl) treated samples. Samples treated with 2.5% NaCl exhibited transition peaks around 70 °C for BC and 79 °C for LC. LC meat showed higher storage modulus (G′) and loss modulus (G″) values than BC meat, suggesting a stronger gel-forming tendency. LC meat gels exhibited higher hardness, cohesiveness, gumminess, and chewiness, and a slightly lower pH (6.14 vs. 5.97) compared to BC meat gels (p < 0.05). LC meat gels displayed larger expressible moisture content (p < 0.05), although the value was approximately 6%. Compared to LC meat gels, BC meat gels appeared slightly whiter (p < 0.05). To compare the lipid oxidation of BC and LC meat gels day by day, the thiobarbituric acid reactive substances (TBARS) of gels stored at 4 °C in polyethylene bags were measured on Days 0, 4, and 8. Both BC and LC meat gels showed acceptable lipid oxidation-based rancid off-flavor after short-term storage at 4 °C, with TBARS values below 2 mg malondialdehyde (MDA) equivalent/kg on Day 8. Understanding these variations in biochemical properties and functional behavior can help optimize processing methods and produce meat products of superior quality that meet consumer preferences.

Long-Term Consequences of Increased Activity of Urine Enzymes After Cardiac Surgery - A Prospective Observational Study.

IntroductionCardiac surgery associated AKI (CSA-AKI) complicates recovery and may be associated with a greater risk of developing chronic kidney disease and mortality. The aim of this study was to assess long-term clinical consequences of transient increased activity of urinary enzymes after cardiac surgery (CS).MethodsAn observational study was conducted in a group of 88 adult patients undergoing planned coronary artery bypass grafting (CABG), but all samples were obtained from 79 patients. The activity of urinary enzymes: N-acetyl-beta-glucosaminidase (NAG), arylsulfatase A (ASA) and beta-glucuronidase was evaluated in sequential urine samples. A comparative analysis of biochemical parameters was performed regarding the occurrence of acute kidney injury (AKI) defined by KIDGO at 24 hours, at day 30 and 5-years after the operation.ResultsDuring the first 24 hours after CS AKI was diagnosed in 13 patients. A comparison of the activity of urinary enzymes in pre-defined time-points showed significant differences for ASA and NAG (post OP-sample p < 0.028 and p < 0.022; POD 1 sample p < 0.004 and p < 0.001 respectively). No patient had any biochemical or clinical features of kidney failure at day 30. In the AKI group kidney failure was diagnosed in 36% of patients within 5 years of follow-up as opposed to 5% in the no AKI group. The activities of tubular enzymes in urine reflect a general injury of kidney tubules during and after the operation. However, they are not ideal biomarkers for prediction of the degree of kidney injury and further poor prognosis of CS-AKI.

A Comparative Analysis of Biochemical Parameters in the Liver of Round Goby Neogobius melanostomus (Pallas, 1814) from Two Regions of the Taganrog Bay (Sea of Azov)

A Comparative Analysis of Biochemical Parameters in the Liver of Round Goby Neogobius melanostomus (Pallas, 1814) from Two Regions of the Taganrog Bay (Sea of Azov)

Study of the ascorbate-glutathione cycle in transgenic plants Nicotiana tabacum under abiotic stress conditions

We conducted a comparative analysis of biochemical parameters for the ascorbate-glutathione cycle in non-transgenic and transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS-gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction of elevated concentrations of copper, chromium, and lead ions into the soil promotes induction of the acdS-gene expression and an increase in ACC-deaminase activity in transgenic plants. It was shown the content of ascorbic acid (АА), glutathione, ascorbate peroxidase (АРХ) and glutathione peroxidase (GP) and glutathione reductase (GR) increased in plants under abiotic stress.

ПОКАЗАТЕЛИ КРОВИ БЫЧКОВ КАЛМЫЦКОЙ ПОРОДЫ НА ОТКОРМЕ ПРИ ВКЛЮЧЕНИИ В ИХ РАЦИОН МЕСТНЫХ ПРИРОДНЫХ КОРМОВЫХ ДОБАВОК В УСЛОВИЯХ ЦЕНТРАЛЬНОЙ ЯКУТИИ

Obtaining high results in the production of highquality beef in beef cattle breeding is impossible without well-organized fattening of gobies. In the conditions of Yakutia, fattening of gobies from 15 months of age can be carried out in autumn, after the summer grazing feeding. Under economic conditions of feeding, high growth rates of fattening young animals cannot be obtained. Therefore, intensive fattening with the use of mineral feeding of cattle is used. The paper presents research materials on the morphological and biochemical composition of blood and its serum in calves of the Kalmyk breed, which are on scientific and industrial fattening. In the experimental group of animals, local natural feed additives – zeolite and salt – were added to the diet, which served as adaptogens and sources of mineral elements. During the feeding period before the slaughter, the morphological composition of blood in terms of indicators has insignificant decreases, but within the physiological norm. We see these changes in the gobies of the experimental control group and associate them with a sharp decrease in atmospheric air temperature to –15 °C in the first ten days of November 2019 and a constant transition to roughage. It is also possible that the use of natural feed additives in the form of feeding zeolite and salt increases metabolic processes in the animal's body. A comparative analysis of biochemical parameters in the blood serum of gobies of the experimental group confirmed significant differences in hepatic enzymes (ALT and AST) in animals. The use of non-traditional natural feed additives with the inclusion in the diet of fattening gobies affects the hematological and biochemical parameters of blood and its serum, and, accordingly, meat quality.

Association of Arterial Hypertension with Hepatobiliary Pathology: The Occurrence of Comorbidity and Features of Metabolic Processes.

Comorbidity of hypertension and hepatobiliary pathology has negative medical and social consequences, including an increase in the indicators of hospital admissions, disability and mortality.ObjectiveThe aim was to study the occurrence of hypertension combined with hepatobiliary diseases depending on social status, gender and age in 2003-2017 and their influence on indicators of metabolic processes in patients with a therapeutic profile.MethodsA cross-sectional study using the inpatients’ medical record database of the clinic of Federal Research Centre for Basic and Translational Medicine (Novosibirsk, Russia), which collects demographics, diagnoses (using ICD-10 codes), procedures and examinations of all inpatients from 2003-2017 was conducted. The incidence of comorbidity of hypertension and hepatobiliary pathology depending on age, gender and social status, based on the analysis of 13496 medical records was examined. A comparative analysis of biochemical parameters characterizing the main types of metabolism (lipid, protein, carbohydrate and purine) was carried out in 3 groups of patients: with hypertension; with hepatobiliary pathology, and with a combined pathology.ResultsDuring the years 2003-2005, there was the greatest frequency of this comorbidity in workers, in women, in the age group 60 years and older. In 2009-2017, the highest incidence was observed in the male administrative staff. In patients with this comorbidity, more pronounced changes in carbohydrate, protein, lipid and purine metabolism were found in comparison with groups of patients with isolated diseases.ConclusionThe results highlight the need to improve the system of prevention and treatment of comorbidity taking into account sex, age, occupation and features of metabolism.

Analysis of the low-molecular weight antioxidants of transgenic plants Nicotiana tabacum under abiotic stress conditions

We conducted a comparative analysis of biochemical parameters of non-transgenic and transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS-gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction of elevated concentrations of copper, chromium, and lead ions into the soil promotes induction of the acdS-gene expression and an increase in ACC-deaminase activity in transgenic plants. It was shown that the content of phenolic compounds (flavonoids), ascorbic acid, the total antioxidant activity of plants increased under abiotic stress.

Comparative analysis of biochemical parameters and gene expressions of novel markers in type II Diabetes mellitus

Background: Diabetic nephropathy (DN) is the most common cause of end stage renal disease (ESRD). Early detection of the disease and treatment of this chronic complication which would reduce the medical and economic burden. Early detection of kidney injury by evaluating gene expressions of Il-6, Il-10, LDLr, and CD36 in T2DM with pre-ESRD microalbuminuria minimizes the risk of DN. Methods: Present research work conducted at the Department of Biochemistry, School of Medicine, Navi Mumbai. This study includes 241 subjects (118 male, 123 women, and age ranges 30-70 years) were included after screening for T2DM by measurement of blood glucose in fasting, post-prandial, glycosylated haemoglobin. Microalbumin in urine and e-GFR is measured to eliminate patients of ESRD. Subjects were recruited after written consent and enrolled as per inclusion/exclusion criteria. Categorization of subjects in three study groups; group I (30-45 years), group II (46-70 years) were done on the basis of T2DM duration 3-6 years, glycosylated haemoglobin level (HbA1c) ≥ 7.0% with fasting blood glucose ≥126 mg/dl) and microalbuminuria (30-300 mg/dl) in study group, equal numbers of healthy volunteers enrolled in control group. Blood samples were processed for other renal parameters and RT-PCR to check expressions of novel genes Results: In study groups all renal, lipids parameters are within normal range except albumin/creatinine ratio (p &lt;0.012), e-GFR (p &lt;0.00) and cholesterol (p &lt;.00). Descriptive analysis showed high significance (p &lt;.00) of delta CT gene expressions, parameters in pre-ESRD microalbuminuria subjects. Conclusion: Screening biochemical renal parameters are not enough to prevent DN even in microalbuminuria. Early detection of gene expressions of novel biomarkers predicts risk of kidney injury. Early intervention may prevent morbidity and mortality of kidney due to diabetic nephropathy.

The Comparative Analysis of Biochemical Parameters in Patients with Pleural Effusions: A Prospective Study

The Comparative Analysis of Biochemical Parameters in Patients with Pleural Effusions: A Prospective Study

Comparative analysis of biochemical parameters in diabetic and non-diabetic acute myocardial infarction patients

Diabetes is a metabolic disorder characterized by enhanced production of free radicals hence oxidative stress. The aim of this study was to evaluate the activity of cardiac and antioxidant enzymes in diabetic and non-diabetic acute myocardial infarction (AMI) patients. This case-control study was conducted on 450 subjects (70-85 years). Subjects were divided into three groups (Normal, N; Non-diabetic AMI, N-AMI; and Diabetic AMI, D-AMI). Each individual was subjected to a detailed history, clinical examination, and cardiovascular parameters analysis (fasting blood sugar, HbA1c, systolic and diasystolic blood pressure, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), TC/HDL and LDL/HDL ratios). Cardiac markers (Troponin-I, creatine phosphokinase (CPK), creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), C-reactive protein (CRP) and aspartate aminotransferase (AST)) and oxidative stress markers (superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), catalase (CAT)) were also assessed. All these parameters were compared between diabetic and non-diabetic AMI patients. D-AMI individuals had high level of TC, TG, LDL, and low level of HDL in comparison to N-AMI individuals. Study suggests that cardiac markers such as Troponin I, CPK, CK-MB, AST, LDH, and CRP levels were significantly increased in patients suffering from myocardial infarction with diabetes mellitus (DM) compared to patients of myocardial infarction without DM. The activity levels of antioxidant SOD and GSH were lower in D-AMI patients than in N-AMI. However, levels of MDA and CAT were higher in D-AMI than in N-AMI controls. Study suggests elevated cardiac markers and reduced antioxidants in D-AMI patients compared to N-AMI patients.

Comparative analysis of biochemical parameters of atherosclerosis adiponectin and resistin in patients with diabetes mellitus and coronary heart disease

Comparative analysis of biochemical parameters of atherosclerosis adiponectin and resistin in patients with diabetes mellitus and coronary heart disease

Comparative analysis of biochemical parameters for differentiation of pleural exudates from transudates: Light's criteria, cholesterol, bilirubin, albumin gradient, alkaline phosphatase, creatine kinase, and uric acid

The differentiation of pleural effusions as being either transudate or exudate is the first step in the diagnosis of pleural effusions. The aim of this study was to compare the efficiency of the various biochemical parameters to the traditional criteria of Light et al., for differentiating exudates from transudates. Ninety-three pleural fluid and sera specimens were obtained and classified as transudates or exudates on the basis of their diagnosis. Of the 93 pleural fluids, 21 were transudates, 72 were exudates. The efficiencies of different parameters for detection of exudates were as follows: The criteria of Light 96%; effusion cholesterol concentration 77%; serum-fluid albumin gradient 67%, pleural/serum alkaline phosphatase ratio 83%; effusion creatine kinase levels 91%; pleural/serum creatine kinase ratio 83%, and effusion uric acid 71%. Pleural/serum uric acid ratio was insignificant for the purpose of this study.