Compact Zone Research Articles

The mechanism and performance of rock breakage by undercutting disc cutter with advanced slotting

To study the mechanism and performance of rock breakage by an undercutting disc cutter with advanced slotting, a three-dimensional numerical model of rock cutting by a disc cutter with advanced slotting assistance was established based on the discrete element method. The parallel bond constitutive model was selected to describe the micromechanical properties of rock. The correctness of the established numerical model is verified through rock breakage experiments, and the rock cutting process by the disc cutter was analyzed by a combination of the force chain and crack distribution. The influencing factors, such as advanced slotting depth, cutting thickness, rock strength, and cutter rotation speed, on rock cutting performance were investigated. The results show that a compact zone is gradually formed between the rock and disc cutter at the beginning, then a large number of microscopic tensile and shear cracks in the compact zone due to micro failure of rock are formed; the subsequent main rock fragment is mainly caused by tensile failure; advanced slotting can reduce the rock bearing capacity and bending resistance, the rock above the advanced slotting fractured easily due to its lower bending resistance, and the volume compact zone is relatively small. When the advanced slotting depth is equal to 12.5 mm, the propulsive force and specific energy consumption of rock cutting by the disc cutter are reduced by 61.6% and 16.5%, respectively. The propulsive force and specific energy consumption increase as the rock strength increases, but they tend to close when the rock strength is greater than 80 MPa, which indicates that advanced slotting assistance is more suitable for hard rock. The results obtained in this paper can provide the operating parameters determination under different factors to some extent of the undercutting disc cutter in a pre-cut condition, which further improve the rock breaking performance of mechanized cutter.

The impact of dust evolution on the dead zone outer edge in magnetized protoplanetary disks

Context. The dead zone outer edge corresponds to the transition from the magnetically dead to the magnetorotational instability (MRI) active regions in the outer protoplanetary disk midplane. It has been previously hypothesized to be a prime location for dust particle trapping. A more consistent approach to access such an idea has yet to be developed, since the interplay between dust evolution and MRI-driven accretion over millions of years has been poorly understood. Aims. We provide an important step toward a better understanding of the MRI–dust coevolution in protoplanetary disks. In this pilot study, we present a proof of concept that dust evolution ultimately plays a crucial role in the MRI activity. Methods. First, we study how a fixed power-law dust size distribution with varying parameters impacts the MRI activity, especially the steady-state MRI-driven accretion, by employing and improving our previous 1+1D MRI-driven turbulence model. Second, we relax the steady-state accretion assumption in this disk accretion model, and partially couple it to a dust evolution model in order to investigate how the evolution of dust (dynamics and grain growth processes combined) and MRI-driven accretion are intertwined on million-year timescales, from a more sophisticated modeling of the gas ionization degree. Results. Dust coagulation and settling lead to a higher gas ionization degree in the protoplanetary disk, resulting in stronger MRI-driven turbulence as well as a more compact dead zone. On the other hand, fragmentation has an opposite effect because it replenishes the disk in small dust particles which are very efficient at sweeping up free electrons and ions from the gas phase. Since the dust content of the disk decreases over millions of years of evolution due to radial drift, the MRI-driven turbulence overall becomes stronger and the dead zone more compact until the disk dust-gas mixture eventually behaves as a grain-free plasma. Furthermore, our results show that dust evolution alone does not lead to a complete reactivation of the dead zone. For typical T-Tauri stars, we find that the dead zone outer edge is expected to be located roughly between 10 au and 50 au during the disk lifetime for our choice of the magnetic field strength and configuration. Finally, the MRI activity evolution is expected to be crucially sensitive to the choice made for the minimum grain size of the dust distribution. Conclusions. The MRI activity evolution (hence the temporal evolution of the MRI-induced α parameter) is controlled by dust evolution and occurs on a timescale of local dust growth, as long as there are enough dust particles in the disk to dominate the recombination process for the ionization chemistry. Once that is no longer the case, the MRI activity evolution is expected to be controlled by gas evolution and occurs on a viscous evolution timescale.

Struktura skalního sesuvu Kutiště vyvinutého v krystaliniku Hrubého Jeseníku

Slope failures developed in crystalline rocks of the Hrubý Jeseník Mts. (Czechia) are quite sparse phenomenon. So far, only a few deep-seated landslides have been described from areas near Keprník Mt. and Červená hora Mt., while shallow flood- related flows are more frequent. The newly identified Kutiště rockslide (GPS 50.1437297N, 17.0563289E) is located in the village of Jindřichov (Šumperk District, the Olomouc Region, Czechia) about 1 km northeast of Štolný hřbet Mt. (883 m a. s. l.) within the Hrubý Jeseník Mts. The rockslide has been geomorphologically mapped and structural analysis and geophysical measurements using electrical resistivity tomography have been carried out to interpret surface and subsurface structure of the landslide. It is a planar rockslide developed in biotitic orthogneiss of the Keprník Nappe, covering an area of approximately 0.13 km2 (ca. 500 × 250 m) with a total altitude difference of about 140 m. The landslide is dominated by a morphologically very distinct amphitheatre-like headscarp. The approximately 300 m long and relatively compact transport zone of the slide is dissected by several transverse fissures and tension cracks, and it is delimited towards the bedrock by a planar sliding surface at depths of 10–17 m. The accumulation part of the landslide, about 150 m long, has further collapsed by gravity. The resistivity image in this part indicates a sliding surface at a depth of about 25 m. The structural data indicate that the slope failure was structurally predisposed by an orthogonal system of longitudinal (NNW-SSE) and transverse (ENE-WSW) brittle structures, and the hillslope-conformal metamorphic foliation of orthogneiss inclined at an angle of 11° towards N to NNW. The resistivity image of the rockslide does not exclude the presence of deeper sliding surfaces throughout the entire extent of the slope deformation, which would enable its further development. The morphologically fresh appearance of the landslide is not necessarily a result of low age, but rather of the strong resistance of the orthogneiss to weathering.

Development of a novel rapid repairing agent for concrete based on GFRP waste powder/GGBS geopolymer mortars

The use of waste glass fiber-reinforced polymer (GFRP) powder as a binding agent in the synthesis of geopolymers offers an eco-friendly solution for managing GFRP waste. The aluminosilicate present in GFRP powder can be activated by an alkaline solution to produce a sustainable geopolymer. In this study, a novel rapid repairing material was developed from partially-replaced ground granulated blast furnace slag (GGBS) by GFRP waste powder as precursors in geopolymer mortar (GM). The effect of GFRP powder replacement and mix proportions on the fresh and hardened properties of GMs were evaluated. It was found that an increase in GFRP powder replacement led to an increase in the setting time and a decrease in workability. The highest 28-day compressive strength was obtained at a 20% replacement of GGBS with GFRP powder. At this optimal mixture, the GMs achieved a compressive strength of 15.2 MPa after 6 h and 35.7 MPa after 1 day. Results from the slant shear test revealed that the failure pattern of the GM-repaired concrete samples changed from interface failure to monolithic failure and explosive spalling in the concrete substrate (CS), when the test duration increased from 3-days to 7-days and 28-days, respectively. This was attributed to the formation of a compact interfacial zone with prolonged testing duration, leading to increased bond strength. The developed GMs demonstrate a short setting time, excellent flowability, high early mechanical strength and superior bond properties, and thus can be applied as rapid concrete repair materials or as protective coatings for concrete in corrosive environments.

Understanding the effect of high-volume fly ash on micro-structure and mechanical properties of cemented coal gangue paste backfill

The utilization of coal gangue and fly ash to produce cemented paste backfill is an effective strategy to mitigate the environmental impact of such bulk solid wastes. In this study, we aimed to investigate the hardening properties of cemented coal gangue paste backfill (CCGPB) containing high-volume fly ash using macroscopic and microscopic characterization techniques. Specifically, uniaxial compressive strength (UCS) and slump were measured to evaluate the hardening properties of CCGPB, while Scanning Electron Microscopy (SEM) and Mercury Intrusion Porosimetry (MIP) were used to characterize the internal microstructure and internal pores. A total of 216 mixture proportions were designed through variations in the contents of cement, fly ash, water, and curing time. The resulting experimental data were used to develop a random forest model. This model was subsequently employed to evaluate the impact of different variables on the development of UCS in CCGPB. The results revealed that the slump increased with increasing fly ash contents and mass concentration. The maximum UCS was achieved at the mass concentration of 0.81 and the fly ash-cement replacement ratio of 0.42, which was also verified by the SEM showing a more compact interfacial transition zone with hydration products of calcium silicate hydrates, calcium aluminosilicate hydrates, and ettringites. The MIP results showed that the addition of fly ash had slight effect on the pores less than 1 μm, while pores larger than 1 μm increased with increasing fly ash content. The porosity ratio decreased by only 4% when fly ash was increased from 30% to 50%. The simulation results by the developed RF model indicated that the curing age had the highest relative importance (47.7%), while the UCS gain was less sensitive to the fly ash content compared with the cement. Overall, this study provides valuable insights into the application of fly ash and coal gangue to cemented paste backfill.

Application value of multiparametric MRI for evaluating iron deposition in the substantia nigra in Parkinson's disease.

This study aimed to investigate the application value of multi-parametric magnetic resonance imaging (MRI) in the diagnosis of iron deposition in the substantia nigra dense zone in Parkinson's disease (PD) and to evaluate the diagnostic value of the correlation among multi-parametric imaging indicators, clinical stage, and disease duration. Thirty-six patients with clinically confirmed PD and 36 healthy controls were enrolled. The disease course was recorded, and PD severity was graded using the Hoehn-Yahr (H-Y) scale. All subjects underwent magnetic sensitivity weighted imaging (SWI), neuromelanin magnetic resonance imaging (NM-MRI), and a T2*mapping sequence. Based on the fusion of the NM-MRI and SWI amplitude maps, phase maps, and T2*MAPPING value maps, NM-MRI was used to delineate the dense zone of the substantia nigra, which was divided into three sub-regions: upper, middle, and lower. In this way, the amplitude, phase, and R2* values of each sub-region and the average value of the sum of the three sub-regions were obtained simultaneously in the SWI amplitude, phase, and T2*MAPPING maps. The multi-parameter imaging indices were compared between the two groups, and the correlation between them and clinical indices was evaluated in the PD group. The upper (amplitude, phase value, R2* value), middle, and lower (amplitude) right substantia nigra compact zones were significantly different between the PD and control groups. The upper (phase value, R2* value) and middle (amplitude) areas of the left substantia nigra compact zone were also significantly different between the two groups (all P < 0.05). The mean values (amplitude, phase value, R2* value) of the right substantia nigra densification zone and the mean values (phase value) of the left substantia nigra densification zone were also significantly different (all P < 0.05). Amplitudes in the middle and lower parts of the right substantia nigra dense zone were negatively correlated with the H-Y grade (middle: r = -0.475, P = 0.003; lower: r = -0.331, P = 0.049). Amplitudes of the middle and lower parts of the dense zone of the left substantia nigra were negatively correlated with the H-Y grade (middle: r = -0.342, P = 0.041; lower: r = -0.399, P = 0.016). The average amplitude of the right substantia nigra compact zone was negatively correlated with the H-Y grade (r = -0.367, P = 0.027). The average R2* value of the compact zone of the left substantia nigra was positively correlated with the H-Y grade (r = 0.345, P = 0.040). Multiparametric MRI sequence examination has application value in the evaluation of iron deposition in the dense zone of the substantia nigra in PD. Combined with NM-MRI, fusion analysis is beneficial for accurately locating the substantia nigra compact zone and quantitatively analyzing the iron deposition in different sub-regions. Quantitative iron deposition in the middle and lower parts of the substantia nigra dense zone may become an imaging biological indicator for early diagnosis, severity evaluation, and follow-up evaluation of PD and is thus conducive for clinical diagnosis and treatment evaluation.

Alpha-klotho and peritoneal membrane status: Ahypothesis generating study.

Long-term success of peritoneal dialysis relies on the integrity of the peritoneal membrane. This proof-of-concept study addressed the hypothesis that fibrosis is already present in the membrane at pre-dialysis and that the membrane status is related to the individual's uraemic fingerprint. A clinical-mechanistic, transversal, single-centre study was conducted. Pre-dialysis peritoneal biopsies were scored considering the submesothelial compact zone thickness (STM), vasculopathy and inflammation. We investigated if the membrane status could be inferred from a panel of proteins (α-Klotho, Galectin-3, FGF21, FGF23, Tweak, TNFα and hsPCR) in blood. A total 58 incident patients aged 56 ± 15 years old were included, 31% female, 55% hypertension, 29% diabetic and 24% obese. Person-to-person STM was found to be highly variable and 38% of patients were fibrosis positive. Both α-Klotho (Spearman r= -.7491, p< 0.001) and FGF21 (Spearman r= -.5102, p< 0.001) were negatively associated with STM. α-Klotho, but not FGF21, was able to discriminate fibrosis from nonfibrosis with/without inflammation and vasculopathy. PLS models identified α-Klotho as the protein most relevant for fibrosis. α-Klotho was independently associated with fibrosis of the peritoneal membrane (OR=.991 (.896-.997), p= 0.002). Before the start of dialysis in incident patients, some patients already present fibrosis of the peritoneal membrane and other patients do not. Our findings suggest that α-Klotho may be implicated in fibrosis of the peritoneal membrane.

Modeling of Daytime and Nighttime Surface Urban Heat Island Distribution Combined with LCZ in Beijing, China

Along with urbanization, surface urban heat island (SUHI) has attracted more attention. Due to the lack of perspective of spatial heterogeneity in relevant studies, it is difficult to propose specific strategies to alleviate the SUHI. This study discusses the impact of spatial heterogeneity on the day and night SUHI by taking one day and night in Beijing as an example, and uses it to improve the efficiency of SUHI simulation for related planning. This study, based on the local climate zone (LCZ), deeply discusses the relationship between urban morphology and the SUHI. Then, an artificial neural network (ANN) model with the LCZ is developed to predict the distribution of the SUHI. The results show that: (1) In summer, the general SUHI intensity distribution patterns are compact zone &gt; large low-rise zone &gt; open zone and medium floor zone &gt; low floor zone &gt; high floor zone. (2) Building density and albedo in dense areas are higher correlated with the SUHI than open areas. The building height has a significant negative correlation with the SUHI in high-rise zone, but has a positive correlation in middle and low floors. (3) The LCZ improves the overall accuracy of the ANN model, especially the simulation accuracy in the daytime. In terms of regions, LCZ2, LCZ8, and LCZ10 are improved to a higher degree. This study is helpful to formulate the SUHI mitigation strategies of “adapting to the conditions of the LCZ” and provide reference for improving the sustainable development of the urban thermal environment.

Mapping urban carbon emissions in relation to local climate zones: Case of the building sector in Bangkok Metropolitan Administration, Thailand

This study focuses on carbon emissions of the building sector in relation to local climate zone (LCZ) classification, concentrating on two major parts. First, we estimated carbon emissions in the building sector, which were calculated for weekdays and weekends real-time daily energy consumption patterns. The estimations were divided into direct (from petroleum products consumption) and indirect emissions (from electricity consumption). Second, we examined urban carbon emissions mapping in relation to LCZ. Bangkok Metropolitan Administration (BMA) was used as the case study and 2016 as the base year for examination. The results illustrate that indirect emissions in Bangkok can be up to ten times higher than direct emissions. The analysis indicates that LCZ, such as compact high-rise, large low-rise, light industry, and warehouse zones had a relatively higher carbon emission intensity than others. Additionally, we identified that the compact high-rise zone has the highest indirect emission intensity, while the light industry and warehouse zone have the greatest direct emission intensity. These results provide insights into the dynamics of carbon emission characteristics in the building sector and the methodology purported here can be used to support low carbon city planning and policymaking in Bangkok.

Asymptotic Measurement Schemes for Every Observable of a Quantum Field Theory

In quantum measurement theory, a measurement scheme describes how an observable of a given system can be measured indirectly using a probe. The measurement scheme involves the specification of a probe theory, an initial probe state, a probe observable and a coupling between the system and the probe, so that a measurement of the probe observable after the coupling has ceased reproduces (in expectation) the result of measuring the system observable in the system state. Recent work has shown how local and causal measurement schemes may be described in the context of model-independent quantum field theory (QFT), but has not addressed the question of whether such measurement schemes exist for all system observables. Here, we present two treatments of this question. The first is a proof of principle which provides a measurement scheme for every local observable of the quantized real linear scalar field if one relaxes one of the conditions on a QFT measurement scheme by allowing a non-compact coupling region. Secondly, restricting to compact coupling regions, we explicitly construct asymptotic measurement schemes for every local observable of the quantized theory. More precisely, we show that for every local system observable A there is an associated collection of measurement schemes for system observables that converge to A. All the measurement schemes in this collection have the same fixed compact coupling zone and the same processing region. The convergence of the system observables holds, in particular, in GNS representations of suitable states on the field algebra or the Weyl algebra. In this way, we show that every observable can be asymptotically measured using locally coupled probe theories.

Integrated molten and vapor condensation of polymeric carbon nitride photoelectrode towards efficient water splitting

Polymeric carbon nitride (PCN) has received extensive attention in suspension photocatalysis, whereas the preparation of high-quality PCN film is still at an initial stage, limiting its practical application in photoelectrochemical (PEC) systems. Herein, an integrated molten and vapor condensation route is realized using small molecule precursors dicyandiamide (DCDA) and thiourea (TU) to form PCN film photoelectrode with high performance. The in-situ deposition route endows the PCN film with a composite structure including a thick porous zone with suitable thickness for light absorption and thin compact zone with a higher C/N ratio for better charge transportations. The growth of the specific film and the functions of precursors DCDA and TU are investigated in detail. Owing to the unique condensation route and film structure, this PCN film exhibits excellent performance in PEC water splitting with a photocurrent density of ca. 140 µA cm−2 in 0.1 mol L−1 NaOH electrolyte at 1.23 VRHE, which is competitive or higher than the results of the reported PCN photoanodes prepared by the molten-mediated method and thermal vapor condensation. The integrated molten and vapor condensation strategy is enlightening for comprehending the growth of PCN photoelectrodes.

Effect of Counter- and Co-Swirl on Low-Frequency Combustion Instabilities of Jet A-1 Spray Flames

Abstract The present article experimentally investigates the influence of pilot swirling directions on low-frequency combustion instabilities of pilot diffusion flames in a laboratory-scale combustor with jet A-1 fuel and air at atmospheric pressure. Airblast atomization nozzles with either counter-rotating (CTR) or corotating (COR) pilot swirl flows were examined using nonlinear time-series analyses and high-speed flame imaging measurements under idle and subidle operating conditions. We show that while the amplitude and frequency of limit cycle oscillations are observed to be similar for both cases, detailed examinations of measured experimental data reveal marked differences in stabilization mechanisms and pressure-heat release coupling processes. The spray flame dynamics subjected to counter-rotating swirl flows are governed by large-amplitude pressure oscillations, even under the influence of destructive pressure-heat release rate interference. The mechanism of destructive interference is closely related to the interactions between a spiral diffusion flame and a periodically detached reaction zone. Nonpremixed liquid-fueled flames involving corotating swirl, on the other hand, feature a more compact and intense reaction zone.

Cumulative Dialytic Glucose Exposure is a Risk Factor for Peritoneal Fibrosis and Angiogenesis in Pediatric Patients Undergoing Peritoneal Dialysis Using Neutral-pH Fluids

Neutral-pH dialysate has been reported to be beneficial to prevent the peritoneal pathological changes in adult peritoneal dialysis (PD) patients, but its use is controversial in pediatric PD patients. In addition, the impact of cumulative dialytic glucose exposure has not been examined. Pediatric PD patients using conventional fluids (conventional group, n= 31) or those using neutral-pH fluids (neutral-pH group, n= 33) were compared. Clinical risk factors for peritoneal pathological changes in the neutral-pH group were analyzed using generalized linear modeling. Furthermore, the mechanisms of peritoneal pathological changes were explored using immunohistochemical studies and cultured cells. The median (interquartile range) duration of dialysis was 3.2 (1.7-5.3) years in overall patients. After propensity score matching, the conventional group showed increased thickening of the submesothelial compact (SMC) zone and lower luminal-to-vessel diameter (L/V) ratio than the neutral-pH group. In the neutral-pH group, the cumulative dialytic glucose exposure was an independent risk factor for greater thickness of the SMC zone (odds ratio [OR], 1.54; 95% confidence interval [CI], 1.16-2.05) and higher submesothelial microvessel density (OR, 1.29; 95% CI, 1.01-1.64). Immunohistochemical study showed that cumulative dialytic glucose exposure correlated with the proportion of the tissue expressing hypoxia inducible factor -1α (HIF-1α) and vascular endothelial growth factor-α (VEGF-α). In human peritoneal mesothelial cells, high glucose significantly increased HIF-1α and VEGF-α expressions. Cumulative dialytic glucose exposure is an independent risk factor for peritoneal fibrosis and angiogenesis in pediatric patients undergoing PD using neutral-pH fluids, which might be associated with greater VEGF-α production by myofibroblasts implying a hypoxic response.

Dual therapy with an angiotensin receptor blocker and a JAK1/2 inhibitor attenuates dialysate-induced angiogenesis and preserves peritoneal membrane structure and function in an experimental CKD rat model.

Peritoneal dialysis (PD) is limited by reduced efficacy over time. We previously showed that a Janus kinase 1/2 inhibitor (JAK1/2i) reduced inflammation, hypervascularity and fibrosis induced by 4.25% dextrose dialysate (4.25%D) intraperitoneally (IP) infused for 10 days in rats with normal kidney function. JAK/STAT signalling mediates inflammatory pathways, including angiotensin signalling. We now tested the effect of long-term JAK1/2i and/or an angiotensin receptor blocker (ARB) on peritoneal membrane (PM) in polycystic kidneys (PCK) rats infused with 4.25%D. Except for controls, all PCK rats had a tunnelled PD catheter: (1) no infusions; (2) 4.25%D; (3) 4.25%D + JAK1/2i (5 mg/kg); (4) 4.25%D +losartan (5 mg/kg); and (5) 4.25%D + losartan +JAK1/2i (5 mg/kg each) IP BID × 16 weeks (N = 5/group). PM VEGFR2 staining areas and submesothelial compact zone (SMCZ) width were morphometrically measured. Peritoneal equilibration testing measured peritoneal ultrafiltration (UF) by calculating dialysate glucose at time 0 and 90 min (D/D0 glucose). 4.25%D caused hypervascularity, SMCZ widening, fibrosis and UF functional decline in PCK rats. Angiogenesis was significantly attenuated by JAK1/2i ± ARB but not by ARB monotherapy. Both treatments reduced SMCZ area. UF was preserved consistently by dual therapy (p < 0.05) but with inconsistent responses by monotherapies. Long-term JAK1/2i ± ARB reduced angiogenesis and fibrosis, and the combination consistently maintained UF. In clinical practice, angiotensin inhibition has been advocated to maintain residual kidney function. Our study suggests that adding JAK1/2i to angiotensin inhibition may preserve PM structure and UF.

Relationship between laparoscopic and microscopic findings of peritoneum in peritoneal dialysis patients

Long-term exposure to the peritoneal dialysis solution (PDS) causes functional and morphological alterations that diminish the efficacy of peritoneal dialysis (PD). Macroscopic and microscopic findings, submesothelial compact zone (SMC) thickness and vascular patency, were associated with PD duration. The relationship between microscopic and laparoscopic morphological findings in PD patients was determined. A total of 78 laparoscopic intraperitoneal findings were recorded during PD catheter removal and 45 peritoneal tissues were obtained from the anterior parietal peritoneum. We examined macroscopic morphological findings in both parietal and visceral peritoneums and bowel movement and assessed the score semiquantitatively. SMC thickness and vascular patency were examined as microscopic findings. Total laparoscopic finding’s score (LFS) and microscopic findings, SMC thickness and vascular patency, were associated with PD duration. Total LFS was related to SMC thickness in both visceral and parietal peritoneum, whereas it was related to vascular patency in parietal but not in visceral peritoneum. There was no relationship between microscopic findings and peritoneal surface color, properties, vasculopathy, and adhesion. Total LFS in patients with newly formed membrane and omentum atrophy was higher than in those without. There was a significant relationship between microscopic and laparoscopic findings in PD patients. It is important to evaluate laparoscopic findings in more PD patients to find the predictive findings of encapsulating peritoneal sclerosis development.

Endothelial Loss of ETS1 Impairs Coronary Vascular Development and Leads to Ventricular Non-Compaction.

Jacobsen syndrome is a rare chromosomal disorder caused by deletions in the long arm of human chromosome 11, resulting in multiple developmental defects including congenital heart defects. Combined studies in humans and genetically engineered mice implicate that loss of ETS1 (E26 transformation specific 1) is the cause of congenital heart defects in Jacobsen syndrome, but the underlying molecular and cellular mechanisms are unknown. To determine the role of ETS1 in heart development, specifically its roles in coronary endothelium and endocardium and the mechanisms by which loss of ETS1 causes coronary vascular defects and ventricular noncompaction. ETS1 global and endothelial-specific knockout mice were used. Phenotypic assessments, RNA sequencing, and chromatin immunoprecipitation analysis were performed together with expression analysis, immunofluorescence and RNAscope in situ hybridization to uncover phenotypic and transcriptomic changes in response to loss of ETS1. Loss of ETS1 in endothelial cells causes ventricular noncompaction, reproducing the phenotype arising from global deletion of ETS1. Endothelial-specific deletion of ETS1 decreased the levels of Alk1 (activin receptor-like kinase 1), Cldn5 (claudin 5), Sox18 (SRY-box transcription factor 18), Robo4 (roundabout guidance receptor 4), Esm1 (endothelial cell specific molecule 1) and Kdr (kinase insert domain receptor), 6 important angiogenesis-relevant genes in endothelial cells, causing a coronary vasculature developmental defect in association with decreased compact zone cardiomyocyte proliferation. Downregulation of ALK1 expression in endocardium due to the loss of ETS1, along with the upregulation of TGF (transforming growth factor)-β1 and TGF-β3, occurred with increased TGFBR2/TGFBR1/SMAD2 signaling and increased extracellular matrix expression in the trabecular layer, in association with increased trabecular cardiomyocyte proliferation. These results demonstrate the importance of endothelial and endocardial ETS1 in cardiac development. Delineation of the gene regulatory network involving ETS1 in heart development will enhance our understanding of the molecular mechanisms underlying ventricular and coronary vascular developmental defects and will lead to improved approaches for the treatment of patients with congenital heart disease.

Tracing a decade of activity towards a yellow hypergiant. The spectral and spatial morphology of IRC+10420 at au scales

ABSTRACT The fate of a massive star during the latest stages of its evolution is highly dependent on its mass-loss history and geometry, with the yellow hypergiants (YHGs) being key objects. We present near-IR interferometric observations of the famous YHG IRC+10420 and blue spectra taken between 1994 and 2019. Our 2.2-μm GRAVITY/VLTI observations attain a spatial resolution of ∼5 stellar radii and spatially resolve the hot emission in the K-band tracing the gas via Na i doublet emission and the Br γ emission. Our geometric modelling reveals a compact neutral zone (Na i) which is slightly larger than the continuum but within an extended Br γ emitting region. Our study confirms an hour-glass geometry of the wind, but we find no signature of a companion at 7–800 au separations at the contrast limit of our observations (3.7 mag at 3σ) to explain this geometry. We report an evolution of the ejecta over 7 yr, and constrain the opening angle of the hour-glass to be &amp;lt;10°. Lastly, we present the first blue optical spectra of IRC+10420 since 1994. The multi-epoch data indicate that the spectral type, and thus temperature, of the object has essentially remained constant during the intervening years. Therefore, the observed increase in temperature of 2000 K in less than two decades prior to 1994 is now halted. This suggests that this YHG has ‘hit’ the White Wall in the HR-diagram preventing it from evolving blue-wards, and will likely undergo a major mass-loss event in the near future.

High sensitivity refractive index sensing using zone plate metasurfaces with a conical phase profile

In this paper, we showed how a bulky Axicon lens can be transformed to a compact binary zone plate with conical phase profile. We built three zone plates made of three different materials and designed each zone plate to be used in high sensitivity refractive index sensing. This work is complementary to another work we have done before in which we showed mathematically how maximum sensitivity can be achieved in case of using an Axicon lens in sensing. The zone plates are designed to generate a Bessel–Gauss beam at the wavelength of 3.3 microns at which the absorption of methane gas is maximum leading to a maximum change in the refractive index. The generated intensity in the output is very sensitive to any slight change in the refractive index of the surrounding medium. Therefore, if an optical detector is positioned at the point of maximum change in the intensity with refractive index, we can easily measure the change in refractive index and hence the percentage of the gas with very high sensitivity.

Geopolymer Concrete with Lightweight Artificial Aggregates.

This article presents the physical and mechanical properties of geopolymer concrete with lightweight artificial aggregate. A research experiment where the influence of fly ash–slag mix (FA-S), as part of a pozzolanic additive, on the properties of geopolymers was carried out and the most favorable molar concentration of sodium hydroxide solution was determined. The values of three variables of the examined properties of the geopolymer lightweight concrete (GLC) were adopted: X1—the content of the pozzolanic additives with fly ash + flay ash–slag (FA + FA-S) mix: 200, 400 and 600 kg/m3; X2—the total amount of FA-S in the pozzolanic additives: 0, 50 and 100%; X3—the molarity of the activator NaOH: (8, 10 and 12 M). In order to increase the adhesion of the lightweight artificial aggregate to the geopolymer matrix, the impregnation of the NaOH solution was used. Based on the obtained results for the GLC’s compressive strength after 28 days, water absorption, dry and saturated density and thermal conductivity index, it was found that the most favorable parameters were obtained with 400 kg/m3 of pozzolanic additives (with 50% FA-S and 50% FA) and 10 NaOH molarity. Changes in the activator’s concentration from 8 to 10 M improved the compressive strength by 54% (for a pozzolana content of 200 kg/m3) and by 26% (for a pozzolana content of 600 kg/m3). The increase in the content of pozzolanic additives from 200 to 400 kg/m3 resulted in a decrease in water absorption from 23% to 18%. The highest conductivity coefficient, equal to 0.463 W/m·K, was determined, where the largest amount of pozzolanic additives and the least lightweight aggregate were added. The structural tests used scanning electron microscopy analysis, and the beneficial effect of impregnating the artificial aggregate with NaOH solution was proved. It resulted in a compact interfacial transition zone (ITZ) between the lightweight aggregate and the geopolymer matrix because of the chemical composition (e.g., silica amount), the silica content and the alkali presoaking process.

Therapeutic Targeting of GSK3β-Regulated Nrf2 and NFκB Signaling Pathways by Salvianolic Acid A Ameliorates Peritoneal Fibrosis.

Peritoneal fibrosis is a devastating complication in patients undergoing peritoneal dialysis, with no definite therapy yet available. Salvia miltiorrhiza and its major active component Salvianolic acid A (Sal A) have demonstrated a beneficial effect in myriad diseases. However, their effect on peritoneal fibrosis is unknown. In murine models of peritoneal dialysis, daily Sal A treatment substantially improved the peritoneal dialysis fluid (PDF) elicited peritoneal fibrosis, marked by thickening of the submesothelial compact zone, accumulation of extracellular matrix and increased expression of vimentin and PAI-1, concomitant with attenuation of GSK3β hyperactivity. This coincided with diminished nitrotyrosine in peritoneal tissues and increased Nrf2 nuclear translocation, entailing a lessened oxidative injury and reinforced Nrf2 antioxidant response. Meanwhile, inflammatory infiltration and maladaptive angiogenesis in peritoneal tissues provoked by PDF injury were also mitigated by Sal A, associated with a suppressed NFκB activation. Mechanistically, ectopic expression of the constitutively active GSK3β blunted the NFκB-suppressing and Nrf2-activating efficacy of Sal A in peritoneal mesothelial cells exposed to hypertonic dextrose, suggesting that GSK3β inhibition mediates the protective effect of Sal A. Collectively, our findings may open the avenue for developing a novel therapy based on Sal A for preventing peritoneal fibrosis in peritoneal dialysis.