Communities Study Research Articles

Development, characterization, and replication of proteomic aging clocks: Analysis of 2 population-based cohorts.

Biological age may be estimated by proteomic aging clocks (PACs). Previous published PACs were constructed either in smaller studies or mainly in white individuals, and they used proteomic measures from only one-time point. In this study, we created de novo PACs and compared their performance to published PACs at 2 different time points in the Atherosclerosis Risk in Communities (ARIC) study of white and black participants (around 75% white and 25% black). A total of 4,712 plasma proteins were measured using SomaScan in blood samples collected in 1990 to 1992 from 11,761 midlife participants (aged 46 to 70 years) and in 2011 to 2013 from 5,183 late-life participants (aged 66 to 90 years). The de novo ARIC PACs were constructed by training them against chronological age using elastic net regression in two-thirds of healthy participants in midlife and late life and validated in the remaining one-third of healthy participants at the corresponding time point. We also computed 3 published PACs. We estimated age acceleration for each PAC as residuals after regressing each PAC on chronological age. We also calculated the change in age acceleration from midlife to late life. We examined the associations of age acceleration and change in age acceleration with mortality through 2019 from all-cause, cardiovascular disease (CVD), cancer, and lower respiratory disease (LRD) using Cox proportional hazards regression in participants (irrespective of health) after excluding the training set. The model was adjusted for chronological age, smoking, body mass index (BMI), and other confounders. We externally validated the midlife PAC using the Multi-Ethnic Study of Atherosclerosis (MESA) Exam 1 data. The ARIC PACs had a slightly stronger correlation with chronological age than published PACs in healthy participants at each time point. Associations with mortality were similar for the ARIC PACs and published PACs. For late-life and midlife age acceleration for the ARIC PACs, respectively, hazard ratios (HRs) per 1 standard deviation were 1.65 and 1.38 (both p < 0.001) for all-cause mortality, 1.37 and 1.20 (both p < 0.001) for CVD mortality, 1.21 (p = 0.028) and 1.04 (p = 0.280) for cancer mortality, and 1.68 and 1.36 (both p < 0.001) for LRD mortality. For the change in age acceleration, HRs for all-cause, CVD, and LRD mortality were comparable to the HRs for late-life age acceleration. The association between the change in age acceleration and cancer mortality was not significant. The external validation of the midlife PAC in MESA showed significant associations with mortality, as observed for midlife participants in ARIC. The main limitation is that our PACs were constructed in midlife and late-life participants. It is unknown whether these PACs could be applied to young individuals. In this longitudinal study, we found that the ARIC PACs and published PACs were similarly associated with an increased risk of mortality. These findings suggested that PACs show promise as biomarkers of biological age. PACs may be serve as tools to predict mortality and evaluate the effect of anti-aging lifestyle and therapeutic interventions.

An integrated strain-level analytic pipeline utilizing longitudinal metagenomic data.

The advancement in DNA-sequencing technology has enabled the high-resolution identification of microorganisms in microbial communities. Since different microbial strains within species may contain extreme phenotypic variability (e.g., nutrition metabolism, antibiotic resistance, and pathogen virulence), investigating within-species variations holds great scientific promise in understanding the underlying mechanism of microbial biological processes. To fully utilize the shared genomic variants across longitudinal metagenomics samples collected in microbiome studies, we develop an integrated analytic pipeline (LongStrain) for longitudinal metagenomics data. It concurrently leverages the information on proportions of mapped reads for individual strains and genome-wide SNVs to enhance the efficiency and accuracy of strain identification. Our method helps to understand strains' dynamic changes and their association with genome-wide variants. Given the fast-growing longitudinal studies of microbial communities, LongStrain which streamlines analyses of large-scale raw sequencing data should be of great value in microbiome research communities.

Phytosociological Study of Weed Communities in Zea Mays Cultivation: Contributions to Sustainable Development Goals (SDG'S) in the Ecuadorian Tropics

Objective: This study investigates the phytosociological characteristics of weed communities associated with Zea mays cultivation in different zones of the Ecuadorian tropics, aiming to identify and classify weeds to develop effective management strategies that enhance productivity while minimizing environmental impacts Theoretical Framework: The research is based on the concept that weeds, or 'noxious weeds,' significantly challenge maize cultivation by competing for essential resources. Theories related to plant competition and agroecosystem sustainability underpin the study Method: The methodology involves a phytosociological survey across five farms in Mocache, Ventanas, and Balzar. Using 45 quadrats of 50 x 50 cm in a zigzag pattern across one-hectare plots, weed species were identified and quantified. Data were analyzed to calculate biodiversity and importance value indices. Results and Discussion: Mocache had the highest weed population, while Balzar exhibited greater species diversity. Similarity indices, including Jaccard and Bray-Curtis, revealed distinct patterns in species distribution and dominance. These findings are discussed in the context of sustainable agriculture, highlighting the implications for weed management and acknowledging potential limitations due to environmental variability. Research Implications: The study provides insights into sustainable weed management in maize agroecosystems, contributing to strategies aligned with SDG 2 (Zero Hunger) and SDG 15 (Life on Land), promoting practices that enhance food security and biodiversity conservation. Originality/Value: This research offers a novel phytosociological analysis of weed communities in tropical maize cultivation, contributing to sustainable agriculture and aligning with global sustainability goals.

Six-year change in high-sensitivity cardiac troponin T with subsequent stroke risk in the general population

BackgroundThe association between change in high-sensitivity cardiac troponin T (hs-cTnT) and stroke risk in the general population remains unknown. We aimed to assess the association of a 6-year change in...

Subsequent risk of cancer among adults with peripheral artery disease in the community: The atherosclerosis risk in communities (ARIC) study

Background and aimsSeveral studies reported an increased cancer risk related to lower-extremity peripheral artery disease (PAD) but had important caveats: not accounting for key confounders like smoking, follow-up <10 years, or no race-specific results. To assess the long-term independent association of PAD with cancer incidence in a bi-racial community-based cohort. MethodsWe categorized 13,106 ARIC participants without cancer at baseline (mean age 54.0 [SD 5.7] years, 45.7 % male, and 26.1 % Black) into symptomatic PAD (clinical history or intermittent claudication), asymptomatic PAD (ankle-brachial index [ABI] ≤0.9), and five ABI categories (0.1-interval between 0.9 and 1.3 and > 1.3). We used cancer registries and medical records to ascertain cancer cases and ran multivariable Cox models. ResultsDuring the median follow-up of 25.3 years, there were 4143 incident cancer cases. 25-year cumulative incidence was 37.2 % in symptomatic PAD, 32.3 % in asymptomatic PAD, and 28.0–31.0 % in the other categories. Symptomatic and asymptomatic PAD remained significantly associated with cancer incidence after adjusting for potential confounders, including smoking and diabetes (hazard ratio [HR] 1.42 [1.05–1.92] and 1.24 [1.05–1.46], respectively). When stratified by smoking status, we observed a robust association of PAD (symptomatic and asymptomatic combined) vs. no PAD with cancer risk in ever smokers (HR 1.42 [1.21–1.67]) but not in never smokers. The results were most evident for lung cancer (HR 2.16 (95 %CI 1.65–2.83) for PAD vs. no PAD within ever smokers). ConclusionsSymptomatic and asymptomatic PAD conferred cancer risk, particularly among ever smokers and for lung cancer. Patients with PAD should receive evidence-based cancer prevention and screening.

The succession of microbial community and distribution resistance gene in response to enrichment cultivation derived from a long-term toxic metal(loid)s polluted soil

Microbial communities as the most important and active component of soil play a crucial role in the geochemical cycling of toxic metal(loid)s in the Pb and Zn smelting site soils. However, the relationships between soil microbial communities and the fractions of toxic metal(loid)s and the succession of soil microbial community and functions after enrichment cultivation have rarely been analyzed. In this study, the diversity and composition of microbial communities in soils before and after enrichment cultivation were investigated by high-throughput sequencing. And the co-occurrence relationships between soil microbial community after enrichment cultivation and MRGs genes were also analyzed through the BacMet database. Results showed that the dominant genus in the soils was Lactobacillus and Stenotrophomonas. The soil microbial community exhibited a notable correlation with Cd, Pb, and As, among which Cd exerted the most profound impact. Alishewanella, Pseudomonas, Massilia and Roseibacillus were significantly correlated with the fraction of Cd. After enrichment cultivation, the number of genera decrease to 96. And the dominant genus changed to Acinetobacter, Bacillus, Comamonas, Lysobacter, and Pseudoxanthomonas. High abundance of metal resistance genes (MRGs) including zntA, fpvA, zipB, cadA, czcA, czcB, czcC, zntA, arsR, pstS and pstB was found in the microbial community after enrichment cultivation. The potential host genus for MRGs was Acinetobacter, Comamonas, Lysinibacillus, Azotobacter, Bacillus, Lysobacter, Cupriavidus, Pseudoxanthomonas, and Thermomonas. Additionally, these microbial community after enrichment cultivation possessing pathways of bacterial chemotaxis and two-component systems was enabled them to adapt to the polluted environment. These observations provided potential guidance for microbe isolation and the development of strategies for the bioremediation of toxic metal(loid)s polluted soils.

Viral metagenomic survey of Caspian seals.

Viral diseases of pinnipeds cause substantial mortality and morbidity and can influence population demography. Viral metagenomic studies can therefore play an important role in pinniped health assessments and disease surveillance relevant to both individual species and in a "One Health" context. This study used a metagenomic approach with high throughput sequencing to make the first assessment of viral diversity in Caspian seals (Pusa caspica), the only marine mammal species endemic to the Caspian Sea. Sequencing libraries from 35 seals sampled 2009-2020 were analysed, finding sequences from the viral families Circoviridae, Parvoviridae, Herpesviridae, Papillomaviridae, Picornaviridae, Caliciviridae, Cruciviridae, Anelloviridae, Smacoviridae, and Orthomyxoviridae, with additional detection of Adenoviridae via PCR. The similarity of viral contigs from Caspian seal to sequences recovered from other pinnipeds ranged from 63.74% (San Miguel sea lion calicivirus) to 78.79% (Seal anellovirus 4). Some findings represent novel viral species, but overall, the viral repertoire of Caspian seals is similar to available viromes from other pinnipeds. Among the sequences recovered were partial contigs for influenza B, representing only the second such molecular identification in marine mammals. This work provides a foundation for further studies of viral communities in Caspian seals, the diversity of viromes in pinnipeds more generally, and contributes data relevant for disease risk assessments in marine mammals.

The piranha gut microbiome provides a selective lens into river water biodiversity

Advances in omics technologies have enabled the in-depth study of microbial communities and their metabolic profiles from all environments. Here metagenomes were sampled from piranha (Serrasalmus rhombeus) and from river water from the Rio São Benedito (Amazon Basin). Shotgun metagenome sequencing was used to explore diversity and to test whether fish microbiomes are a good proxy for river microbiome studies. The results showed that the fish microbiomes were not significantly different from the river water microbiomes at higher taxonomic ranks. However, at the genus level, fish microbiome alpha diversity decreased, and beta diversity increased. This result repeated for functional gene abundances associated with specific metabolic categories (SEED level 3). A clear delineation between water and fish was seen for beta diversity. The piranha microbiome provides a good and representative subset of its river water microbiome. Variations seen in beta biodiversity were expected and can be explained by temporal variations in the fish microbiome in response to stronger selective forces on its biodiversity. Metagenome assembled genomes construction was better from the fish samples. This study has revealed that the microbiome of a piranha tells us a lot about its river water microbiome and function.

Crafting shape-changing experiences: emotional evaluation of morphing metamaterials

ABSTRACT We introduce a novel approach to evaluate how the design properties of morphing metamaterial influence shape-changing experiences. We leverage parametric design to create cutting patterns that change the Density and Geometry material properties. Using laser-cutting, we apply the patterns to a non-stretchable woven textile, resulting in a stretchable metamaterial that we actuate using an electromagnetic coil. This systematic design approach leads to programmatic control over the material movement, making it suitable for controlled evaluation of perceived experience. In a 2×2 mixed-method study, we assess how the metamaterial’s Density and Geometry properties impact the observed experience. Our findings indicate that such changes significantly and independently affect participants’ perceived emotions, mood, associations, and preferences. Our research contributes to the body of work of systematic studies in the shape-changing community. By using our morphing metamaterial design and implementation technique, coupled with our controlled evaluation of specific design properties, we have revealed new insights about the influencing factors of shape-changing experiences. We have incorporated these insights into our morphing metamaterial design guidelines, which outline the impact of Density and Geometry properties on an observer’s perceived emotions. We hope our work will open up new possibilities for expressive, evocative metamaterial-focused shape-changing experiences.

Association of left atrial function with frailty: The Atherosclerosis Risk in Communities (ARIC) study.

Frailty is common in people with cardiovascular disease. Worse left atrial (LA) function is an independent risk factor for cardiovascular disease. However, whether worse LA function is associated with frailty is unclear. We included 3292 older adults from the Atherosclerosis Risk in Communities study who were non-frail at baseline (visit 5, 2011-2013) and had LA function (reservoir, conduit, and contractile strain) measured from two-dimensional speckle-tracking echocardiography. LA stiffness index was calculated as a ratio of E/e' to LA reservoir strain. Frailty was defined using the validated Fried frailty phenotype. Incident frailty was assessed between 2016 and 2019 during two follow-up visits. LA function was analyzed as quintiles. Multivariable logistic regression examined odds of incident frailty. Median (interquartile range [IQR]) age was 74 (71-77) years, 58% were female, and 214 (7%) participants developed frailty during a median (IQR) follow-up of 6.3 (5.6-6.8) years. After adjusting for baseline confounders and incident cardiovascular events during follow-up, the odds of developing frailty was 2.42 (1.26-4.66) times greater among participants in the lowest (vs highest) quintile of LA reservoir strain and 2.41 (1.11-5.22) times greater among those in the highest (vs lowest) quintile of LA stiffness index. Worse LA function was significantly associated with the development of exhaustion, but not the other components of the Fried frailty phenotype. Worse LA function is associated with higher incidence of frailty and exhaustion component independent of LA size and left ventricular function. Future studies are needed to elucidate the underlying mechanisms that drive the observed association.

Affective incapacity of the depressed self: Evidence from a narrative study of an online depression community on Weibo

With the increasing prevalence of depression worldwide, there is a noticeable number of online depression communities (ODCs) flourishing in cyberspace. This phenomenon offers a discursive platform for observing and discerning people’s mental and affective struggles. Under the overarching framework of narrative analysis, we coded the most salient emotions expressed in 2000 pieces of comments by the top 20 core participants of ‘Zoufan’, an ODC space on China Weibo. We analyze how the members construct their emotive struggles and depressed self in self-talk (disengagement) and interactions (engagement) via linguistic deixis. Based on this communication mapping, this study offers a new notion of affective incapacity as an epistemological insight into depressive emotions. The observed phenomena of both inward-centered (self-talk) emotions and outward-centered (dialogue) emotions point to members’ reduced capacity to affect and to be affected, to intervene and to be intervened. This research is expected to educate both the public and social professionals in recognizing more nuanced emotional deliberation of self-reported depression and take early measures if need be.

Investigating the Slow Food - Place relation: An empirical study of dairy communities in Abruzzo

The paper examines the relational processes characterizing the life of Slow Food communities (or presidia) of dairy producers in the Southern Italian region of Abruzzo. The study uses the notion of assemblage to theoretically approach and empirically analyse these processes. The paper investigates the links between Slow Food and the different actors involved in the development of the presidia, thus providing an interpretation of the communities as the result of complex participatory processes. It also explores the relations in place between presidia and then the links between various products, localities, and food traditions. Through this analysis, the paper demonstrates how the territories considered are linked by the presence of interdependent food productions and by the existence of strong connections between different groups of activists and producers. The study contributes to the understanding of the dynamic and composite nature of Slow Food in terms of its relationships with other similar assemblages and with the territories in which the movement operates.

The effect of organizational uprightness behaviors on organizational brilliance through the mediation of pioneer vigilance: analytical research of the opinions of a sample of administrators at the national retirement authority - Baghdad

The study aimed to determine the effect of Organizational Uprightness Behaviors with its dimensions (empathy, trust, integrity, optimism) in Organizational Brilliance with its dimensions (leadership Brilliance, service Brilliance, creativity, knowledge Brilliance) by mediating the Pioneer Vigilance with its dimensions (survey and research, cooperation and communication, evaluation, and judgment) in the National Retirement Authority in Baghdad. The descriptive analytical approach was adopted for the purpose of the study in the research community, by distributing a questionnaire to a random sample of (169) individuals suitable for analysis from the total community of (328) individuals, represented by a group of employees in the Authority, by adopting the questionnaire as a primary tool in collecting data Statistical programs were used to analyze the data, including (Smart Pls 4, SPSS, v.28, AOMS, and V26). The research attempted to answer several questions that express the research problem, the most important of which was: Is there an impact of organizational uprightness behaviors in achieving organizational Brilliance mediated by pioneer vigilance? The importance of applying the research in the Iraqi National Retirement Authority lies in the fact that it is one of the critical organizations that target a broad segment of Iraqi society who have spent their youth-serving the country and who deserve to find the best ways to serve them and complete their transactions with all respect and appreciation, and facilitate procedures as quickly as possible. The validity of the hypotheses that stated the existence of a significant and statistical effect of organizational uprightness behaviors on organizational Brilliance was proven, and pioneer vigilance has a clear role in enhancing the size of this effect through partial mediation. The results indicate that the number and quality of publications in Organizational Uprightness Behaviors, Pioneer Vigilance, Organizational Brilliance contribute deeply to enriching and rooting this trend, in addition to the need to encourage platforms and climates for research and authorship in this vital and relevant field.

Tilia trees are preferred hosts of several ectomycorrhizal Ascomycota – New insights supported by the first community study of the endemic Tilia kiusiana

Asia is the center of Tilia (Malvaceae) species diversity. However, the ectomycorrhizal (ECM) relations of Asian Tilia are scarcely studied. This study provides the first description of ECM communities of Tilia kiusiana, a rare tree endemic to southwestern Japan. Across the natural distribution of T. kiusiana, ECM fungal communities hosted by the species were investigated in three study sites: Hyōgo (H) and Yamaguchi (Y) on Honshu Island, and Ōita (O) on Kyushu Island. Using molecular methods, T. kiusiana was revealed to host a high diversity of fungi belonging to 25 ECM lineages (16, 17, and 14 lineages on sites H, Y, and O, respectively). The ECM communities of T. kiusiana and the previously studied endemic T. japonica were compared to the available data on other ECM tree genera in Japan, revealing that three Ascomycota ECM lineages (/genea-humaria, marcelleina-peziza gerardii, /tuber-helvella) show a preference towards Tilia hosts. While the total lineage richness did not differ among the compared host tree genera, Tilia communities exhibited higher effective lineage richness at higher (less sensitive to rare taxa) diversity orders. Despite their relative rarity, Tilia trees might play an important role in mixed forests: maintaining the diversity of ECM Ascomycota and potentially buffering the ECM fungal biodiversity loss in cases of ECM tree host diversity decline.

Long-term longitudinal analysis of 4,187 participants reveals insights into determinants of clonal hematopoiesis

Clonal hematopoiesis of indeterminate potential (CHIP) is linked to diverse aging-related diseases, including hematologic malignancy and atherosclerotic cardiovascular disease (ASCVD). While CHIP is common among older adults, the underlying factors driving its development are largely unknown. To address this, we performed whole-exome sequencing on 8,374 blood DNA samples collected from 4,187 Atherosclerosis Risk in Communities Study (ARIC) participants over a median follow-up of 21 years. During this period, 735 participants developed incident CHIP. Splicing factor genes (SF3B1, SRSF2, U2AF1, and ZRSR2) and TET2 CHIP grow significantly faster than DNMT3A non-R882 clones. We find that age at baseline and sex significantly influence the incidence of CHIP, while ASCVD and other traditional ASCVD risk factors do not exhibit such associations. Additionally, baseline synonymous passenger mutations are strongly associated with CHIP status and are predictive of new CHIP clone acquisition and clonal growth over extended follow-up, providing valuable insights into clonal dynamics of aging hematopoietic stem and progenitor cells. This study also reveals associations between germline genetic variants and incident CHIP. Our comprehensive longitudinal assessment yields insights into cell-intrinsic and -extrinsic factors contributing to the development and progression of CHIP clones in older adults.

Dysbiosis of the gut microbiota is associated with in-hospital mortality in patients with antibiotic-associated diarrhoea: A metagenomic analysis

BackgroundThe increasing incidence of antibiotic-associated diarrhoea (AAD) is a serious health care problem. Dysbiosis of the gut microbiota is suspected to play a role in the pathogenesis of AAD, but its impact on the clinical outcomes of patients remains unclear. MethodsBetween May and October 2022, 210 patients with AAD admitted to a university hospital and 100 healthy controls were recruited. DNA extraction from stool specimens and shotgun sequencing were performed. Machine learning was conducted to assess profiling at different taxonomic levels and to select variables for multivariable analyses. ResultsPatients were classified into two groups: Clostridioides difficile infection (CDI, n = 39) and non-CDI AAD (n = 171). The in-hospital mortality rate for the patients was 20.0%, but the presence of C. difficile in the gut microbiota was not associated with mortality. Machine learning showed that taxonomic profiling at the genus level best reflected patient prognosis. The in-hospital mortality of patients was associated with the relative abundance of specific gut microbial genera rather than alpha-diversity: each of the five genera correlated either positively (Enterococcus, Klebsiella, Corynebacterium, Pseudomonas, and Anaerofustis) or negatively (Bifidobacterium, Bacteroides, Streptococcus, Faecalibacterium, and Dorea). Genes for vancomycin resistance were significantly associated with in-hospital mortality in patients with AAD (adjusted hazard ratios, 2.45; 95% CI, 1.20–4.99). ConclusionThis study demonstrates the potential utility of metagenomic studies of the gut microbial community as a biomarker for prognosis prediction in AAD patients.

Sex Differences in Hypertension and Its Management Throughout Life.

The prevalence of hypertension and uncontrolled hypertension may differ by age and sex. We included participants in the Atherosclerosis Risk in Communities study at seven study visits over 33 years (visit 1: 15 636 participants; mean age, 54 years; 55% women), estimating sex differences in prevalence of hypertension (systolic blood pressure ≥130 mm Hg; diastolic blood pressure ≥80 mm Hg; or self-reported antihypertension medication use) and uncontrolled hypertension (systolic blood pressure ≥140 mm Hg or diastolic blood pressure ≥90 mm Hg) using unadjusted and comorbidity-adjusted models. The prevalence of hypertension increased from 40% (ages, 43-46 years) to 93% (ages, 91-94 years). Within hypertensive individuals, the prevalence of uncontrolled hypertension was higher in men (33%) than women (23%) at ages 43 to 46 years but became higher in women than men starting at ages 61 to 64, with 56% of women and 40% men having uncontrolled hypertension at ages 91 to 94. This sex difference was not explained by differences in coronary heart disease, diabetes, body mass index, estimated glomerular filtration rate, number of antihypertension medications, classes of medications, or adherence to medications. In both sexes, uncontrolled hypertension was associated with a higher risk for chronic kidney disease progression (hazard ratio, 1.5 [1.2-1.9]; P=4.5×10-4), heart failure (hazard ratio, 1.6 [1.4-2.0]; P=8.1×10-7), stroke (hazard ratio, 2.1 [1.6-2.8]; P=1.8×10-8), and mortality (hazard ratio, 1.5 [1.3-1.6]; P=6.2×10-19). Sex differences in the prevalence of hypertension and uncontrolled hypertension vary by age, with the latter having implications for health throughout the life course.

Associations of multiple serum metals with the risk of metabolic syndrome among the older population in China based on a community study: A mediation role of peripheral blood cells

Metal exposure has been reported to be associated with metabolic syndrome (MetS), however, the evidence remains inconclusive, particularly in elderly individuals. From May to July 2016, serum levels of 16 metals were measured using inductively coupled plasma mass spectrometry (ICP-MS) in 852 elderly individuals (≥65 years) residing in Wuhan, China. Biological detection and disease recognition were based on individual surveys conducted during health check-ups. Spearman’s rank correlation analysis was performed to identify the correlation among serum metals. The data were Ln-transformed to fit a normal distribution for further analyses. Linear and logistic regression were applied to explore the associations between metals and diseases. Restricted cubic spline (RCS) analysis was utilized to examine dose-response relationships. The Weighted Quantile Sum (WQS) score was applied to determine the empirical weights of each heavy metal in the context of their combined effect on metabolic diseases. The prevalence of MetS, hypertension, diabetes, and hyperlipidemia were 46.36 %, 68.90 %, 24.65 %, and 21.60 %, respectively. Serum metal mixture was positively associated with the prevalence of MetS (OR = 1.92, 95 % CI: 1.30–2.82), hypertension (OR = 1.50, 95 % CI: 1.01–2.23), and diabetes (OR = 2.18, 95 % CI: 1.48–3.22). In single metal models, we found that serum zinc levels were associated with an increased risk of MetS, while rubidium had a protective effect against MetS. Interestingly, different metals had distinct effects on specific diseases in this study: lithium and barium were more likely to influence blood pressure, while selenium had a more significant effect on blood glucose. Lipids were more susceptible to the effects of zinc, selenium, and strontium. Platelet count (PLT) and lymphocyte count (LYM) mediated the association between selenium exposure and hyperlipidemia, while neutrophil count (NEU) mediated the relationship between serum rubidium exposure and MetS. Our findings offer valuable etiological insights into the relationship between serum heavy metals and the prevalence of MetS, suggesting that peripheral blood cells may play a mediating role in this association.

Non-Marathi Hindutva Pop Culture and the Imagery of Shivaji: A Digital Ethnographic Exploration of YouTube Contents and Communities

The presence of cultural materials across Internet platforms should not be solely seen as diffused and disconnected propaganda materials directed towards a passive target audience. Instead, they should be seen as elements representing a community and as having the potential to form a dynamic and active community of their own through their presence in certain digital spaces through certain channels. Hence, a study of such online communities and their constituent cultural materials, together with their interrelation with the physical community, can help us to understand the actual social phenomenon marking such cultural production. In this background, the aim of this article is to understand the ways through which the image of Shivaji is employed in Hindutva cultural discourses, along with the reasons which account for the large-scale emotional appeal of the figure of Shivaji, particularly in the non-Marathi cultural landscape. This is done through an analysis of certain popular YouTube channels, their contents and the communities they appear to form. For this purpose, the article is divided into two parts: While part one surveys a few selected YouTube channels, their communities and contents, the second part analyses and contextualises the data thus gathered within the larger historiographical debates concerning Shivaji, to argue that the reason behind the popular emotional appeal and acceptability of the figure of Shivaji in Hindutva cultural discourse emanates from the sociocultural and historical ambivalence associated with his character, where he represents a fresh new revolutionary force against external onslaught as well as against internal orthodoxy. It is argued that it is this ambivalence which has made the figure of Shivaji emotionally appealing and hence acceptable to a wide section of the Hindu populace, allowing each of them to interpret, appropriate and relate with the figure of Shivaji in its own way.

Exploring naphthalene vapor biofiltration: performance and microbial community dynamics with diverse inoculums

AbstractBackgroundNaphthalene is a polycyclic aromatic hydrocarbon, highly dangerous for human health. It is produced as a byproduct of incomplete combustion of organic material and is frequently present in the air. Biofilters offer an effective alternative for its treatment. The aim of this work was to study the treatment of naphthalene vapors through biofiltration using two biofilters: one inoculated with a consortium composed of Fusarium solani and Rhodococcus erythropolis (BF1), and the other inoculated with a consortium of microbial isolates obtained from a previous biofilter eliminating naphthalene vapors.ResultsThe results demonstrate that inoculating a biofilter with a reconstructed consortium of microbial isolates from a naphthalene vapor‐eliminating biofilter allowed a reduction of the startup time from 35 to 5 days, while maintaining a consistent removal capacity (6 g m−3 h−1, equivalent to 80% removal efficiency). It was also observed that the biofilter inoculated with the reconstructed consortium exhibited comparable robustness to a biofilter previously operated for 4 months with naphthalene, with a maximum removal capacity of 14 g m−3 h−1 for a naphthalene inlet load of 17 g m−3 h−1. The study of microbial communities indicates an increase in the bacterial variability, while fungal variability remains low, with Fusarium solani being predominant at 97%.ConclusionsResults obtained during the startup of both biofilters and by challenging biofilters to increasing naphthalene concentrations or decreasing empty bed residence time showed that startup time can be reduced sevenfold by selecting the microbial consortium. An equivalent performance, in the long run, was achieved for both biofilters. © 2024 Society of Chemical Industry (SCI).