Scientific Community Research Articles

YouTube and the implementation and discontinuation of the oral contraceptive pill: A mixed-method content analysis.

Women living in high-quality healthcare systems are more likely to use oral contraceptives at some point in their lives. Research findings have sparked controversial discussions about contraception in the scientific community and the media, potentially leading to higher rates of method discontinuation. Understanding the underlying motives for method discontinuation is crucial for reproductive health equity and future programming interventions. To address this question, this study aims to explore women's experiences of oral contraceptive use and discontinuation on YouTube. A concurrent explanatory mixed-methods design was used to conduct content analysis of German YouTube videos. The information from 175 videos of 158 individuals was extracted through quantitative descriptive content analysis. Twenty-one individuals were included in the qualitative content analysis. The body was a recurring theme in the pill biographies. Women described, for example, bodily sensations as reasons for taking and stopping the pill. They also described positive and negative side effects while taking the pill and after stopping. The most common side effects of taking the pill mentioned by YouTubers were mood swings (76/158), weight gain (45/158), headaches (33/158), and depressed mood (45/158). The symptoms after discontinuation reported most were facial skin impurities (108/158), decreased mood swings (47/158), hair loss (42/158), and weight loss (36/158). Overall, women overwhelmingly rated their discontinuation experience as positive (87/91). The study identified key symptoms of oral contraceptive initiation and discontinuation by portraying the experiences of female YouTubers, adding valuable insights to the understanding of method initiation and discontinuation. Further research is needed to explore women's personal experiences with method discontinuation beyond the YouTube platform.

The IMP research on business networks: a systematic literature review and research agenda

AbstractMore than 40 years of Industrial Marketing and Purchasing (IMP) research have revealed that in b2b markets, business exchange is carried out in continuous buyer–seller relationships. This involves direct and indirect interaction and dynamics resulting in significant related social and material investments and in extensive interdependencies that confer on the business landscape a market-as-network structure. Since the introduction of this ideas, research on business networks has been richly alimented by researchers of the IMP community. Yet, we do not have a clear overview of what this literature has covered in the last twenty years (in the period 2002–2022), in parallel with the many changes that have affected business landscape. With this study, we aim to examine what is the status of the last 20 years of IMP literature specifically dealing with the business networks level of analysis (45 articles) and to offer key directions for imminent research in this domain while analyzing and synthesizing extant literature.

Guanidine Hydrochloride-Induced Hepatitis B Virus Capsid Disassembly Hysteresis.

Hepatitis B virus (HBV) displays remarkable self-assembly capabilities that interest the scientific community and biotechnological industries as HBV is leading to an annual mortality of up to 1 million people worldwide (especially in Africa and Southeast Asia). When the ionic strength is increased, hepatitis B virus-like particles (VLPs) can assemble from dimers of the first 149 residues of the HBV capsid protein core assembly domain (Cp149). Using solution small-angle X-ray scattering, we investigated the disassembly of the VLPs by titrating guanidine hydrochloride (GuHCl). Measurements were performed with and without 1 M NaCl, added either before or after titrating GuHCl. Fitting the scattering curves to a linear combination of atomic models of Cp149 dimer (the subunit) and T = 3 and T = 4 icosahedral capsids revealed the mass fraction of the dimer in each structure in all the titration points. Based on the mass fractions, the variation in the dimer-dimer association standard free energy was calculated as a function of added GuHCl, showing a linear relation between the interaction strength and GuHCl concentration. Using the data, we estimated the energy barriers for assembly and disassembly and the critical nucleus size for all of the assembly reactions. Extrapolating the standard free energy to [GuHCl] = 0 showed an evident hysteresis in the assembly process, manifested by differences in the dimer-dimer association standard free energy obtained for the disassembly reactions compared with the equivalent assembly reactions. Similar hysteresis was observed in the energy barriers for assembly and disassembly and the critical nucleus size. The results suggest that above 1.5 M, GuHCl disassembled the capsids by attaching to the protein and adding steric repulsion, thereby weakening the hydrophobic attraction.

Influence of COVID-19 on depression and anxiety

COVID-19 is an acute respiratory infection caused by the coronavirus SARS-CoV-2, potentially serious, highly transmissible and globally distributed. The consequences of the human body's exposure to the virus have been a focus of great interest for the scientific community. This study aimed to evaluate the effects of COVID-19 on depression and anxiety, with the aim of verifying the presence or absence of worsening of these neuropsychiatric diseases as a result of virus infection. To this end, the tool used was a questionnaire with 16 closed and open questions on the topic, prepared through the Google Forms platform, which was made available through the social networks WhatsApp, Instagram and Facebook, becoming available between the months of February and December of 2022. The questionnaire included questions related to the patient's clinical condition as well as whether or not anxiety and depression worsened. A total of 70 people met the inclusion criteria and responded to the questionnaire, of which 64.29% were diagnosed with anxiety, 10% with depression and 25.71% with both conditions. Of these, 55.71% persisted with neuropsychiatric conditions and were undergoing treatment, while 27.14% were without treatment and 17.14% had their treatments completed. The most effective treatments involved psychotherapy associated with pharmacological therapy. The worsening of symptoms in the post-infection period was 62.86%. An increase in the dosage of psychotropic medications was observed in 87.8% of cases, with the most serious cases being related to the time of infection and possible deleterious effects of the virus on the CNS. The results together corroborate data from the literature on the worsening of psychiatric conditions due to COVID-19.

Evolving Concepts of the SCORE System: Subtracting Cholesterol from Risk Estimation: A Way for a Healthy Longevity?

The role of cholesterol, mainly low-density lipoproteins (LDL-C), as a causal risk factor for atherosclerotic cardiovascular disease (ASCVD) is now established and accepted by the international scientific community. Based on this evidence, the European and American guidelines recommend early risk stratification and “rapid” achievement of the suggested target according to the risk estimation to reduce the number of major cardiovascular events. Prolonged exposure over the years to high levels of LDL-C is one of the determining factors in the development and progression of atherosclerotic plaque, on which the action of conventional risk factors (cigarette smoking, excess weight, sedentary lifestyle, arterial hypertension, diabetes mellitus) as well as non-conventional risk factors (gut microbiota, hyperuricemia, inflammation), alone or in combination, favors the destabilization of the atherosclerotic lesion with rupture/fissuration/ulceration and consequent formation of intravascular thrombosis, which leads to the acute clinical manifestations of acute coronary syndromes. In the current clinical practice, there is a growing number of cases that, although extremely common, are emblematic of the concept of long-term exposure to the risk factor (LDL hypercholesterolemia), which, not adequately controlled and in combination with other risk factors, has favored the onset of major cardiovascular events. The triple concept of “go lower, start earlier and keep longer!” should be applied in current clinical practice at any level of prevention. In the present manuscript, we will review the current evidence and documents supporting the causal role of LDL-C in determining ASCVD and whether it is time to remove it from any score.

Are plain language summaries more readable than scientific abstracts? Evidence from six biomedical and life sciences journals.

In recent decades, members of the general public have become increasingly reliant on findings of scientific studies for decision-making. However, scientific writing usually features a heavy use of technical language, which may pose challenges for people outside of the scientific community. To alleviate this issue, plain language summaries were introduced to provide a brief summary of scientific papers in clear and accessible language. Despite increasing attention paid to the research of plain language summaries, little is known about whether these summaries are readable for the intended audiences. Based on a large corpus sampled from six biomedical and life sciences journals, the present study examined the readability and jargon use of plain language summaries and scientific abstracts on a technical level. It was found that (1) plain language summaries were more readable than scientific abstracts, (2) the reading grade levels of plain language summaries were moderately correlated with that of scientific abstracts, (3) researchers used less jargon in plain language summaries than in scientific abstracts, and (4) the readability of and the jargon use in both plain language summaries and scientific abstracts exceeded the recommended threshold for the general public. The findings were discussed with possible explanations. Implications for academic writing and scientific communication were offered.

A Framework Model of Mining Potential Public Opinion Events Pertaining to Suspected Research Integrity Issues with the Text Convolutional Neural Network Model and a Mixed Event Extractor

With the development of the Internet, the oversight of research integrity issues has extended beyond the scientific community to encompass the whole of society. If these issues are not addressed promptly, they can significantly impact the research credibility of both institutions and scholars. This article proposes a text convolutional neural network based on SMOTE to identify short texts of potential public opinion events related to suspected scientific integrity issues from common short texts. The SMOTE comprehensive sampling technique is employed to handle imbalanced datasets. To mitigate the impact of short text length on text representation quality, the Doc2vec embedding model is utilized to represent short text, yielding a one-dimensional dense vector. Additionally, the dimensions of the input layer and convolution kernel of TextCNN are adjusted. Subsequently, a short text event extraction model based on TF-IDF and TextRank is proposed to extract crucial information, for instance, names and research-related institutions, from events and facilitate the identification of potential public opinion events related to suspected scientific integrity issues. Results of experiments have demonstrated that utilizing SMOTE to balance the dataset is able to improve the classification results of TextCNN classifiers. Compared to traditional classifiers, TextCNN exhibits greater robustness in addressing the problems of imbalanced datasets. However, challenges such as low information content, non-standard writing, and polysemy in short texts may impact the accuracy of event extraction. The framework can be further optimized to address these issues in the future.

PALEOPARASITOLOGICAL CONTRIBUTIONS TO THE STUDY ON ANCIENT INFECTIONS OF HOMINIDS AND OTHER VERTEBRATES IN BRAZIL: A REVIEW

Paleoparasitology is an interdisciplinary science that studies the parasitic infections in the past, using fossils and subfossils recovered from archeological and paleontological lithostratigraphic units. During the 20th and 21st centuries, it has improved the biological knowledge on helminths and protozoans, as well as provided numerous sociocultural insights into the past civilizations. Here, we present an updated review of the paleoparasitological contributions to the analyses of ancient infections in vertebrate remains recovered from different regions of Brazil. Since its emergence, the Paleoparasitology has been important to a multidisciplinary knowledge related to sociocultural aspects of the American Pre-Columbian civilizations and may provide an important ecological, evolutionary, and biogeographical understanding of parasites of vertebrates other than humans throughout the geological time. The present data compilation suggests a prominent role in the scientific community assumed by paleoparasitological approaches, since they have been contributing to a better understanding of helminth and protozoan distribution in South America, during the Phanerozoic eon and consolidating the Paleoparasitology as a global science. Keywords: Coprolite, mummy, helminth, protozoa.

Introducing the progress monitoring tools of the EU Biodiversity Strategy for 2030

The European Union’s Biodiversity Strategy for 2030 (EU BDS) represents a pivotal step forward in the commitment to protect and restore biodiversity, not only within Europe but also at a global scale. In order to reinforce its implementation, an innovative biodiversity knowledge governance has been established. It includes, among other measures, a progress monitoring system that fosters transparency and can inform corrective action to be taken when progress is being reviewed. In this context, we introduce the official, publicly available, online tools at the core of the EU BDS progress monitoring system: the actions tracker and the dashboard. The actions tracker is specifically designed to track progress on the implementation of the more than a hundred actions stemming from the EU BDS, while the dashboard monitors progress across 16 targets using indicators. However, while the actions tracker is a mature tool, the work on the dashboard is still in progress, as indicators are missing for several targets. New scientific input is needed to propel policy tracking and ensure transparent and data-driven monitoring of the EU BDS targets. With this paper, we invite the scientific community to seize this opportunity to actively participate in the policy monitoring process.

ECG screening and Sudden Cardiac Death prevention in endurance athletes

Abstract Introduction Sudden cardiac death (SCD) is the leading cause of mortality in athletes during sport activity. Identifying non-invasive and repeatable risk markers during annual athletes screening remains a crucial goal for the scientific community for the prevention of cardiovascular disease. The ECG, exercise test and spirometry are part of the annual screening program in the sport population over 40 years of age. The Heart Rate Variability (HRV) has been shown to be an independent predictor of mortality in populations with CV disease, but its relationship with SCD has been little explored in those at low risk, including athletes. This study aims to use a 5 minute ECG screening in a specific population of athletes practicing endurance sports and evaluate its clinical repercussions. Aims The primary aim of the study is to assess whether morphological and spectral ECG analysis in endurance athletes by the 5-minute 'mini Holter ECG' can add information to the annual screening for the prevention of CV diseases and SCD. Materials The study population were selected from Endurance Marathon athletes. A control group was also collected for comparison. The 5 minutes ECG was recorded at rest with the Kardia Mobile 6L™ device that use three electrodes, which allows 6 leads recording. HRV indices were obtained using the Kubios software. A morphological analysis was performed and the HRV parameters were analyzed in the time and frequency domains. Results A total of 554 subjects (220 athletes-334 controls, 325 male-229 women) were analyzed. A higher incidence of sinus arrhythmias, LAFB, 1stdegree AVB, J point/ST-segment abnormalities in athletes compared to controls was observed. Athletes had a significantly shorter QTc than the control group (394.87±30.55 ms vs. 407.01±29.09 ms), suggestive of greater protection from malignant ventricular arrhythmias. The HRV PNS (-0.73±1.25 vs -0.21±1.45) and SNS (1.71±1.91 vs 0.58 ± 1.43) indices were also higher and lower in the athlete group than in the control group, suggestive for higher vagal tone. Given the absence of a normality range of standard HRV parameters in the athlete population, values in the normal range of the 5th to 95th percentile were extrapolated in the whole population for the main HRV parameters. The project, therefore, is to evaluate patients with HRV values higher or lower than 5-95 percentile to identify those deserving further clinical investigations and closer follow-up for suspected increased risk of CV disease and SCD. Conclusions The 5-minute cardiac Holter, combining a more accurate analysis in terms of morphology and arrhythmias than the 12-lead ECG, allows a greater number of static and dynamic alterations to be detected; moreover, the analysis of HRV could provide even more information, such that it could be considered as a complementary tool during the examination and FUP of athletes. Further long-term prospective studies are needed to correlate alterations in HRV and clinical events.

Changes in Convective Precipitation Reflectivity over the CONUS Revealed by High-Resolution Radar Observations from 2015 to 2021

The change in extreme precipitation events in the conterminous United States (CONUS) has been of interest to the research communities in recent years for its intensification under environmental and climate change. Previous studies have not yet used sub-hourly precipitation observations to examine convective precipitation change over the CONUS. This study aims to fill the gap by examining convective precipitation, identified by radar reflectivity, in the CONUS using the state-of-the-art Multi-radar Multi-sensor data, operated at the NOAA/National Severe Storms Laboratory, with an unprecedentedly high spatial (1 km) and temporal (2 min) resolutions. These high-resolution data are expected to better capture the precipitation peak and the precipitation pattern. The results showed that in CONUS, precipitation reflectivity increased both in magnitude and the number of convective days from 2015 to 2021. For example, in 2019, 60% of areas showed an increase in the magnitude of precipitation, and the average number of convective days over CONUS has increased by 19%. Changes in precipitation also vary by season and region. This study highlights the need for continued monitoring and understanding of the evolving pattern of extreme precipitation in the CONUS, especially at sub-hourly frequency, as it exposes significant impacts on various sectors, including agriculture, infrastructure, and human health.

What is the name of Spanish hotels? An analysis of the naming of hotels in Benidorm and Alicante

The importance of branding in tourism and hospitality has been the subject of study by the scientific community. However, there is little specific work on brand names in this sector. It is necessary to delve deeper into the predominant naming criteria in the names of hotels and other types of tourist accommodations. We have analyzed the hotel company name in a sample of 212 tourist accommodations, mostly SMEs and family businesses. The results indicate that, regarding the chosen naming criteria, there is mainly a difference between the criteria chosen to name hotels and those chosen to name other types of tourist accommodations, with the latter being more descriptive and less suggestive or evocative. The most recurring naming criterion in the sector is “fantasy” or “creative”. This research aims to expand knowledge about the choice of corporate name in the hospitality sector, based on the typology established by the scientific community, so that it can serve as a reference point for future research on naming or branding.

Critical outcomes to be included in the Core Outcome Set for nutritional intervention studies in older adults with malnutrition or at risk of malnutrition: a modified Delphi Study.

As part of the development of an agreed minimum set of outcomes or Core Outcome Set (COS) for future nutritional intervention trials in older adults with malnutrition or at risk of malnutrition, this work reports on the Delphi surveys and final consensus. Outcomes from a scoping review were incorporated into a two-round Delphi survey. Researchers and healthcare professionals experienced in malnutrition in older adults were invited to take part in an online survey to rate 38 selected outcomes on a nine-point Likert scale ranging from 'not important' to 'critical' for their setting (community, hospital, or long-term care). Consensus for inclusion was reached when ≥75% (or ≥60% if a patient-reported outcome) of the participants scored the outcome as 'critical' and <15% as 'not important'. Resulting outcomes were voted for inclusion or exclusion in the COS in a final online consensus meeting. Ninety-three and 72 participants from diverse professional backgrounds and countries participated in the 1st and 2nd Delphi round, respectively. After both rounds eleven outcomes met the inclusion criteria, largely irrespective of setting. Fifteen participants, representing academia, health care, health policy, industry, and PPI, voted in a final online consensus meeting resulting in ten outcomes: malnutrition status, dietary intake, appetite, body weight or BMI, muscle strength, muscle mass, functional performance, functional limitations, quality of life, and acceptability of the intervention. Ten outcomes will form the COS which is intended to be used by the scientific community in all future nutritional intervention studies for older adults with malnutrition or at risk of malnutrition. The subsequent phase will establish the appropriate methods to measure these outcomes.

Thinking About the Future of Cognitive Remediation Therapy Revisited: What Is Left to Solve Before Patients Have Access?

In our previous paper on the Future of Cognitive Remediation published more than 10 years ago, we envisaged an imminent and wide implementation of cognitive remediation therapies into mental health services. This optimism was misplaced. Despite evidence of the benefits, costs, and savings of this intervention, access is still sparse. The therapy has made its way into some treatment guidance, but these documents weight the same evidence very differently, causing confusion, and do not consider barriers to implementation. This paper revisits our previous agenda and describes how some challenges were overcome but some remain. The scientific community, with its commitment to Open Science, has produced promising sets of empirical data to explore the mechanisms of treatment action. This same community needs to understand the specific and nonspecific effects of cognitive remediation if we are to provide a formulation-based approach that can be widely implemented. In the last 10 years we have learned that cognitive remediation is not "brain training" but is a holistic therapy that involves an active therapist providing motivation support, and who helps to mitigate the impact of cognitive difficulties through metacognition to develop awareness of cognitive approaches to problems. We conclude that, of course, more research is needed but, in addition and perhaps more importantly at this stage, we need more public and health professionals' understanding of the benefits of this therapy to inform and include this approach as part of treatment regimens.

C2c: Predicting Micro-C from Hi-C

Motivation: High-resolution Hi-C data, capable of detecting chromatin features below the level of Topologically Associating Domains (TADs), significantly enhance our understanding of gene regulation. Micro-C, a variant of Hi-C incorporating a micrococcal nuclease (MNase) digestion step to examine interactions between nucleosome pairs, has been developed to overcome the resolution limitations of Hi-C. However, Micro-C experiments pose greater technical challenges compared to Hi-C, owing to the need for precise MNase digestion control and higher-resolution sequencing. Therefore, developing computational methods to derive Micro-C data from existing Hi-C datasets could lead to better usage of a large amount of existing Hi-C data in the scientific community and cost savings. Results: We developed C2c (“high” or upper case C to “micro” or lower case c), a computational tool based on a residual neural network to learn the mapping between Hi-C and Micro-C contact matrices and then predict Micro-C contact matrices based on Hi-C contact matrices. Our evaluation results show that the predicted Micro-C contact matrices reveal more chromatin loops than the input Hi-C contact matrices, and more of the loops detected from predicted Micro-C match the promoter–enhancer interactions. Furthermore, we found that the mutual loops from real and predicted Micro-C better match the ChIA-PET data compared to Hi-C and real Micro-C loops, and the predicted Micro-C leads to more TAD-boundaries detected compared to the Hi-C data. The website URL of C2c can be found in the Data Availability Statement.

#1413 High performance of ACLARA calculator of rat creatinine clearance for the chronic follow up of renal function

Abstract Background and Aims A neural network-based calculator to estimate rat creatinine clearance (ClCr), as a measurement of the glomerular filtration rate (GFR), from paired plasma creatinine concentration and body weight data has been recently developed [1]. Like human GFR estimating formulae, ACLARA averts long experimental procedures and reduces animal stress (by obviating the use of individual isolation in metabolic cages). This tool is publicly available for free use among the scientific community (https://idal.uv.es/aclara/). When collated with experimentally measured ClCr, ACLARA performs very well at comparing experiments in the whole, showing almost identical behavior of all experimental groups and conditions. Yet, ACLARA was trained mostly with data from short experiments involving acute changes in renal function, and from young rats. Fewer data from older animals was included in the development of the calculator. Thus, its field performance on longer evolution of renal function in adult rats has not yet been sufficiently validated. Accordingly, the aim of this work was to deepen in the assessment of ACLARA in the long-term evolution of rat ClCr. Method The evolution of measured (mClCr) versus ACLARA-estimated ClCr (eClCr) was compared at different time points over a period of 3 months in 20 male Wistar rats with chronic renal damage and 8 age-matched controls. In total, 176 data pairs of mClCr and eClCr were obtained. On the one hand, mClCr was determined by the usual experimental procedure [2]. Briefly, rats were allocated in metabolic cages to obtain 24-hour urine samples and urine flow (UF). Creatinine concentration was measured in the urine (Cru) and plasma (Crp) by a colorimetric assay based on the Jaffe's reaction, and mCrCl was then calculated as Cru x UF / Crp. On the other hand, eClCr was estimated with the ACLARA calculator from the corresponding Crp and body weight data. A Pearson's correlation study between mClCr and eClCr was carried out. Results Our results reveal that the information provided by the 90-day evolution of mClCr and eClCr is virtually identical at the whole experiment level (see Fig. 1). Furthermore, ACLARA seems to provide slightly more coherent and homogeneous information, as the considerable experimental error introduced by urine collection is averted in eClCr but present in mClCr. At the individual data level, ACLARA-estimated values are slightly lower than the corresponding measurements. The correlation between mClCr and eClCr was highly significant (r Pearson= 0.83; p &amp;lt; 0.0001). Conclusion ACLARA shows high performance at estimating CrCl in rats of growing age. Our results support its use also in chronic studies in which renal function must be evaluated. Funding This research was funded by grants from Instituto de Salud Carlos III (ISCIII), Ministerio de Ciencia e Innovación (PI21/01226 and PI21/00548 co-funded by the European Union; and RICORS2040, RD21/0005/0004, co-funded by the European Union – NextGenerationEU, Mecanismo para la Recuperación y la Resiliencia (MRR)) and from Consejería de Educación, Junta de Castilla y León (IES160P20), co-funded by FEDER funds. Joana Mercado-Hernández is recipient of a predoctoral fellowship from the Junta de Castilla y Leon (Spain) and the European Social Fund from the European Commission.

Accurate Protein pKa Prediction with Physical Organic Chemistry Guided 3D Protein Representation.

Protein pKa is a fundamental physicochemical parameter that dictates protein structure and function. However, accurately determining protein site-pKa values remains a substantial challenge, both experimentally and theoretically. In this study, we introduce a physical organic approach, leveraging a protein structural and physical-organic-parameter-based representation (P-SPOC), to develop a rapid and intuitive model for protein pKa prediction. Our P-SPOC model achieves state-of-the-art predictive accuracy, with a mean absolute error (MAE) of 0.33 pKa units. Furthermore, we have incorporated advanced protein structure prediction models, like AlphaFold2, to approximate structures for proteins lacking three-dimensional representations, which enhances the applicability of our model in the context of structure-undetermined protein research. To promote broader accessibility within the research community, an online prediction interface was also established at isyn.luoszgroup.com.

Differences in presentation, diagnosis and management of heart failure in women. A scientific statement of the Heart Failure Association of the ESC.

Despite the progress in the care of individuals with heart failure (HF), important sex disparities in knowledge and management remain, covering all the aspects of the syndrome, from aetiology and pathophysiology to treatment. Important distinctions in phenotypic presentation are widely known, but the mechanisms behind these differences are only partially defined. The impact of sex-specific conditions in the predisposition to HF has gained progressive interest in the HF community. Under-recruitment of women in large randomized clinical trials has continued in the more recent studies despite epidemiological data no longer reporting any substantial difference in the lifetime risk and prognosis between sexes. Target dose of medications and criteria for device eligibility are derived from studies with a large predominance of men, whereas specific information in women is lacking. The present scientific statement encompasses the whole scenario of available evidence on sex-disparities in HF and aims to define the most challenging and urgent residual gaps in the evidence for the scientific and clinical HF communities.

The effect of silver nanoparticles and preparations of various pharmacological groups on the bactericidal activity of &lt;i&gt;Staphylococcus aureus&lt;/i&gt;

Multidrug-resistant bacteria have become one of the most serious threats to public health worldwide. The abuse of antibiotics has contributed to the emergence and transmission of resistance mechanisms among bacteria, jeopardizing the therapeutic potential of antibiotics. Uncontrolled use of drugs leads to the formation of antibiotic resistance due to mutations in chromosomal DNA, as well as the production of plasmids, integrons from other bacteria during horizontal gene transfer. In 2010, the BRICS countries (Brazil, Russia, India, China and South Africa) accounted for 76% of antibiotic consumption, with India consuming 12.9 billion units and China – 10 billion units. As of 2017, carbapenem-resistant Acetobacter baumannii and Enterobacteriaceae resulted in approximately US$ 281 million in healthcare costs in the United States. According to numerous reports from the Center for Disease Control and Prevention, approximately 2.3 million episodes of multidrug-resistant microbial diseases resulting in 25,000 deaths are recorded annually in the United States alone. In this regard, the world community of scientists has intensified the study of the combined use of various antibacterial drugs to achieve maximum bactericidal activity. Studies have been conducted to determine the synergistic effect when using combinations of drugs of various pharmacological groups and silver nanoparticles. A significant increase in bactericidal activity by 53.87 times (from 2.528 to 0.0098 mcg/ml) was found with the combined use of AgNPs and DSMO against the reference strain Staphylococcus aureus ATCC 25953. While the cultivation of St. aureus isolate with DSMO and silver nanoparticles revealed a sensitivity increase of 128.2 times (from 5.056 to 0.039 mcg/ml).

Summer undergraduate biomedical research program for underrepresented minority students in a rural, low-income state

IntroductionDiversity can enhance the agenda and quality of biomedical research, but a dearth of underrepresented minorities and women serve as biomedical researchers. The study purpose was to examine the impact of the a summer undergraduate research program on self-efficacy in research, scientific communication, and leadership as well as scientific identity, valuing objectives of the scientific community, and intent to pursue a biomedical research career.MethodsUnderrepresented minority and female undergraduate students participated in a mentored research experience in a rural, low-income state.ResultsStudents' self-efficacy in research, scientific communication, and leadership as well as scientific identity, valuing objectives of the scientific community, and intent to pursue a biomedical research career increased post-program compared to pre-program.ConclusionThis study supports implementation of a biomedical summer undergraduate research program for URM and women in a poor, rural, settings.