Community Members Research Articles

ScyNet: Visualising interactions in community metabolic models

Abstract Motivation Genome-scale community metabolic models are used to gain mechanistic insights into interactions between community members. However, existing tools for visualising metabolic models only cater to the needs of single organism models. Results ScyNet is a Cytoscape app for visualising community metabolic models, generating networks with reduced complexity by focusing on interactions between community members. ScyNet can incorporate the state of a metabolic model via fluxes or flux ranges, which is shown in a previously published simplified cystic fibrosis airway community model. Availability and Implementation ScyNet is freely available under an MIT licence and can be retrieved via the Cytoscape App Store (apps.cytoscape.org/apps/scynet). The source code is available at Github (github.com/univieCUBE/ScyNet).

Attitude of Community Members towards the Capacity of the Village Land Councils in managing land use conflicts between farmers and pastoralists in Tanzania: A cross sectional Case study Analysis.

Village Land Councils have been established to resolve land use conflicts in rural Tanzania. The history reveals that Tanzania has been mostly affected with the endless land based conflicts between farmers and pastoralists.The presence of VLCs in rural Tanzania has brought significant changes in rural areas where farmers and pastoralists compete to utilize and possess scarce resources. However, rural dwellers have different opinions on the worthness of the respective local organs in resolving land use conflicts between farmers and pastoralists. This study therefore intended to explore attitudes from the community members towards the capacity of the Village Land Councils in managing land use conflicts between farmers and pastoralists. Sem-structured interviews were used to collect data where semantic differential scale was used as the appropriate tool for exploring attitudes from the respondents particularly: farmers and pastoralists who mostly rely on VLCs for mediation services. NVivo software was used for analyzing such data. The results indicated that majority of respondents (farmers and pastoralists) in Mbarali and Kilosa District Councils had positive attitude towards VLCs due to their performance in resolving land use conflicts between farmers and pastoralists. Similar response was obtained from the farmers operating in Tunduru District Council. However, some pastoralists in Tunduru District Councils had remained skeptical on the capacity of the VLCs to resolve conflicts and create conducive working environment for both conflicting parties. Their doubts relied on injustice practices such as corruption, nepotism and tribalism that were directly associated with VLC members. Based on the findings, the study suggests for more capacity building programs for enhancing the capacity of VLC members to act firmly and objectively and for the community members so that they develop a sense of responsibilities in supporting the VLCs’ operations for the common good.

Nonnative pest establishment: Spatial patterns and public detection

Early detection of new pests can reduce their long-term impacts by enabling more rapid management response. Knowledge of pest establishment risk and background rates of detection (e.g., by the public) can help inform more cost-effective targeting of formal early detection survey programs. Here we quantify county-level locational attributes associated with pest establishment and detection by the public using data for 113 new pest incursions detected in the United States from 2010 through 2018. Aligning with expectations, we find a higher likelihood of new pest establishment in counties with higher human population numbers, nearer to ports (<250 km), and with amenable climate characteristics. Controlling for potential sample selection issues, we find that pests are less likely to be first detected by the public (e.g., homeowners, community members) versus by other sources (e.g., agency surveys, researchers, or agricultural operators) in counties with higher total crop sales values and lower human population number. The negative association between public detection and high agricultural values may reflect greater survey efforts by other sources (e.g., by agency surveillance programs, researchers, and agricultural operators) in high-value agricultural areas. The positive association between public detection and human population size may reflect larger numbers of public detectors (i.e., people) available to encounter the pests. Our models provide spatially explicit estimates of the likelihood of new pest establishment across U.S. counties and of the likelihood that an established pest would first be detected by the public. These estimates can serve as quantitative inputs to decision-support activities for new pest surveillance planning.

Redesigning Resettlement Homes for Internally Displaced Persons (IDPs) in Borno State

This study proposes a culturally informed redesign of resettlement homes for Internally Displaced Persons (IDPs) in Borno State, Nigeria. The existing housing designs often overlook the cultural values and nuances of the Kanuri people, the predominant ethnic group in the region. The research utilizes a mixed-methods approach, incorporating qualitative insights from interviews and focus groups with Kanuri community members, and data from surveys assessing current housing satisfaction, combining traditional Kanuri architectural features, spatial layouts, and social practices to create culturally responsive resettlement homes. This design intervention aims to enhance the emotional well-being, social cohesion, and resilience of IDPs in their new settlements. The study explores the impact of culturally sensitive design on the emotional well-being, social cohesion, and resilience of IDPs in their new settlements. Results indicate that incorporating traditional Kanuri design principles can significantly enhance the liveability and acceptance of resettlement homes, fostering a supportive environment that respects and preserves cultural identity. The findings underscore the importance of cultural considerations in architectural design for displaced populations, advocating for policies and practices that prioritize cultural relevance in housing solutions. This paper contributes to the broader discourse on humanitarian architecture and the role of culturally responsive design in improving the quality of life for displaced communities.

Oil exporters and the challenges ahead: the role of NOCs in energy transitions

Abstract While constructing pathways to achieving energy transitions is challenging to all global community members, this challenge is even greater for oil-exporting countries. Oil-exporting countries and their national oil companies (NOCs) are key players in the energy transitions. These actors’ existing capacities (financial, technical, human) must be leveraged to accelerate decarbonization processes that are scaled and globally deployed. Energy transition roadmaps must embrace all relevant actors, including oil companies, as stakeholders that can significantly contribute to the acceleration of energy transitions across the globe. This situation must be reflected in outlooks and assessments of energy transitions. This article studies the regulatory conditions and interventions that have made the evolution of several NOCs possible as relevant actors in the 21st-century landscape, considering their activities in traditional energy sources, decarbonization solutions, and new energy sources. These NOCs vary with some having greater flexibility in shaping policies while others needing to comply with regulatory standards. All NOCs in this study, ADNOC, ARAMCO, and the NNPCL were entrusted with a degree of autonomy in addressing climate change, yet the larger margin of discretion ADNOC and ARAMCO corporations enjoyed has allowed them to excel in shaping and implementing these policies. The focus of this paper is on the role of soft law in this change, particularly ESG standards, as leading energy transition governance tools, which introduce accountability and environmental principles that guide the activity of these corporate actors beyond bear minimum requirements in laws and regulations.

Developing a microbial community structure index (MCSI) as an approach to evaluate and optimize bioremediation performance.

Much attention is placed on organohalide-respiring bacteria (OHRB), such as Dehalococcoides, during the design and performance monitoring of chlorinated solvent bioremediation systems. However, many OHRB cannot function effectively without the support of a diverse group of other microbial community members (MCMs), who play key roles fermenting organic matter into more readily useable electron donors, producing corrinoids such as vitamin B12, or facilitating other important metabolic processes or biochemical reactions. While it is known that certain MCMs support dechlorination, a metric considering their contribution to bioremediation performance has yet to be proposed. Advances in molecular biology tools offer an opportunity to better understand the presence and activity of specific microbes, and their relation to bioremediation performance. In this paper, we test the hypothesis that a specific microbial consortium identified within 16S ribosomal ribonucleic acid (rRNA) gene next generation sequencing (NGS) data can be predictive of contaminant degradation rates. Field-based data from multiple contaminated sites indicate that increasing relative abundance of specific MCMs correlates with increasing first-order degradation rates. Based on these results, we present a framework for computing a simplified metric using NGS data, the Microbial Community Structure Index, to evaluate the adequacy of the microbial ecosystem during assessment of bioremediation performance.

Enhancing the capacity of community health workers in prevention and control of epidemics and pandemics in Wakiso district, Uganda: evaluation of a pilot project

BackgroundCommunity Health Workers (CHWs) play a crucial role in outbreak response, including health education, contact tracing, and referral of cases if adequately trained. A pilot project recently trained 766 CHWs in Wakiso district Uganda on epidemic and pandemic preparedness and response including COVID-19. This evaluation was carried out to generate evidence on the outcomes of the project that can inform preparations for future outbreaks in the country.MethodsThis was a qualitative evaluation carried out one year after the project. It used three data collection methods: 30 in-depth interviews among trained CHWs; 15 focus group discussions among community members served by CHWs; and 11 key informant interviews among community health stakeholders. The data was analysed using a thematic approach in NVivo (version 12).ResultsFindings from the study are presented under four themes. (1) Improved knowledge and skills on managing epidemics and pandemics. CHWs distinguished between the two terminologies and correctly identified the signs and symptoms of associated diseases. CHWs reported improved communication, treatment of illnesses, and report writing skills which were of great importance including for managing COVID-19 patients. (2) Enhanced attitudes towards managing epidemics and pandemics as CHWs showed dedication to their work and more confidence when performing tasks specifically health education on prevention measures for COVID-19. (3) Improved health practices such as hand washing, vaccination uptake, and wearing of masks in the community and amongst CHWs. (4) Enhanced performance in managing epidemics and pandemics which resulted in increased work efficiency of CHWs. CHWs were able to carry out community mobilization through door-to-door household visits and talks on community radios as part of the COVID-19 response. CHWs were also able to prioritize health services for the elderly, and support the management of patients with chronic diseases such as HIV, TB and diabetes by delivering their drugs.ConclusionsThese findings demonstrate that CHWs can support epidemic and pandemic response when their capacity is enhanced. There is need to invest in routine training of CHWs to contribute to outbreak preparedness and response.

Mental health priorities and cultural-responsiveness of the Mental Health First Aid (MHFA) training for Asian immigrant populations in Greater Boston, Massachusetts

BackgroundAsians and Asian Americans have the lowest rate of mental health service utilization (25%) in the US compared to other racial/ethnic groups (39 − 52%), despite high rates of depression, anxiety, and suicidal ideation. The lack of culturally-responsive mental health trainings hinders access to mental health services for these populations. We assessed the mental health priorities of Asian communities in Greater Boston and evaluated cultural responsiveness of the Mental Health First Aid (MHFA), a first-responder training teaching participants skills to recognize signs of mental health and substance use challenges, and how to appropriately respond.MethodsThis is community-based participatory research with the Boston Chinatown Neighborhood Center (BCNC), Asian Women For Health (AWFH), and the Addressing Disparities in Asian Populations through Translational Research (ADAPT) Coalition. We conducted focus groups with community-based organization staff and community members to assess mental health priorities of Asian populations in Boston, MA. We then evaluated the utility and cultural-responsiveness of the English-language MHFA for Asian populations through pre- and post-training questionnaires and focus groups with community participants. Paired t-tests were used to evaluate questionnaire responses. Thematic analysis was used to analyze interviews.ResultsIn total, ten staff and eight community members participated in focus groups, and 24 community members completed the MHFA and pre- and post-training questionnaires. Common mental health challenges in the Asian communities reported by participants were loneliness, high stigma around mental illnesses, academic pressure, and acculturation stress. Compared to pre-training, MHFA participants demonstrated lower personal mental health stigma (p < 0.001) and higher mental health literacy (p = 0.04) post-training. Participants also noted the lack of data statistics and case studies relevant to Asian populations in the training, and desired the training be offered in languages spoken by Asian ethnic subgroups (e.g., Chinese, Vietnamese).ConclusionCultural-responsiveness of the MHFA for Asian populations could be improved with the inclusion of data and case studies that capture common mental health challenges in the Asian communities and with translation of the MHFA to non-English languages predominant in Asian communities. Increasing the cultural relevance and language accessibility of the MHFA could facilitate wider adoption of these trainings across communities and help to reduce mental health stigma and gaps in literacy and service utilization.

Qualitative Analysis of Bullying among Adolescents: A Teacher’s View

Bullying is a social problem that usually increases during adolescence and can have serious consequences on the wellbeing of students. One of the members of the educational community that is most present in these situations is the teachers. This qualitative research aims to know the teachers’ perceptions about bullying among adolescents within the school environment. It was possible to deepen the experiences of 20 teachers by conducting semistructured interviews with open-ended questions. The results obtained indicate that education professionals have been bystanders to episodes of bullying among their students and that they opt for mediation between both parties (bully and victim) as an intervention measure. Teachers believe that bullied students tend to be young people with low self-esteem, introverted and sad. On the contrary, bullies are shown to be leaders with social support, egocentric, and with low empathy. The need to know the different perceptions and strategies used by teachers in situations of bullying among their students is discussed, with the intention of promoting resources and programs that can help them in this type of situation.

“I would be so proud of you guys and myself:” Exploring guided play learning interactions among Black caregivers and their children within a Black history home learning intervention during the COVID-19 pandemic

Existing literature on Black caregiver’s interactions with their children has overwhelmingly focused on parenting deficits and interventions designed to “fix” Black families. In utilizing the BlackCreate framework (2023), this study explores how Black caregivers intentionally crafted learning spaces for their children within the context of a six month intervention. Brilliant Joy in a Box was a six-month intervention developed in partnership with a Black woman entrepreneur that delivered caregiver-child Black history home learning boxes to families during the COVID-19 pandemic with the goal of addressing the impacts of persistent historical educational inequities for Black youth, the disproportionate impact of the pandemic Black families, and requests from community members seeking additional programming during the winter months. In the current study, we analyzed videos of 77 families interacting during a guided play activity. We found that the learning spaces created by Black families focused on four subject matters and their domains: literacy, scientific curiosity, math, and identity. We also found that Black caregivers organically utilized guided play to promote joyful interaction and learning. Findings underscore the importance of educators and school staff learning from families in order to reimagine learning and design community based interventions.

Double stigma: a cross-sectional study of Lassa patients with hearing loss in North Central Nigeria

IntroductionLassa fever is a zoonotic infectious disease endemic in West Africa with a high case-fatality rate and reported stigmatization of surviving patients. This study examines discrimination among survivors of Lassa fever (LF) complicated by hearing loss (HL).MethodsThis cross-sectional qualitative study used an in-depth interview guide to collect information from patients with HL about their experience of stigma. Interviews were conducted by a trained team of interviewers at the Jos University Teaching Hospital between January and April 2022 in Hausa language after informed consent was obtained. Recordings of the interviews were transcribed and translated from Hausa to English. Data analysis was conducted using NVivo software using a thematic framework approach.ResultsMost (73%) respondents were male (n = 11); 27% were female (n = 4). The median age was 35 years (interquartile range, 16.5). Some Lassa fever patients experienced stigma and discrimination (53%) including isolation and withdrawal of family and community support during and after illness. HL increased stigma, as some patients were labeled “deaf” by other community members, increasing perceived stigma and devaluation. HL affected the socio-economic wellbeing of some who could not communicate well with their families and customers and constrained social interactions, evoking pain and apathy. Some survivors of LF and victims of its sequelae of HL experienced double stigmatization. While they were ill with LF, a third of respondents reported avoidance and isolation by family and community members who withdrew care and support both to them and their close family members. These forms of stigmatization strained their relationships.ConclusionThere is a need to address stigma in LF survivors who develop HL through concerted community-owned awareness to improve their quality of life along with a robust social support system to aid prevention.

Community perception and experience of health services in the midst of an earthquake and COVID-19 pandemic: A phenomenological study

Indonesia is located on the Pacific Ring of Fire with various disasters occurring frequently and causing tremendous impacts. Recently, Mamuju Regency in West Sulawesi was hit by an earthquake during the COVID-19 pandemic, making primary health care (PHC) a major community health care provider facing several issues. This study aims to explore the challenges and supporting factors of the recovery of PHC after the earthquake during the COVID-19 pandemic using a descriptive phenomenology grounded in Edmund Husserl's philosophy. The Bamboo PHC (Puskesmas Bambu) was selected as the only PHC in Mamuju which was most affected by the earthquake. A focus group discussion method was conducted by involving 15 participants selected using purposive sampling and divided into three groups (health workers, health cadres, and community members). As a result, the community members acknowledged that the Bamboo PHC services programs in general were favorable before the pandemic, there were several basic and superior health service programs that the community felt were valuable. Yet, the COVID-19 pandemic worsened the situation and the use of healthcare facilities decreased. Coupled with the occurrence of natural disasters in vulnerable areas, it is increasingly causing fatalities. Therefore, disaster preparedness and community empowerment are the key to successful disaster management.

Analysis of Risk Factors and The Impact of Nitric Oxide (NO) and Carbon Monoxide (CO) Levels on Lung Function: A Comparative Study of Palm Oil Factory Workers and the Community in Kuantan Mudik District, Riau, Indonesia

Background: Air pollution, particularly from industrial sources like palm oil factories, poses a significant risk to respiratory health. This study aimed to investigate the impact of nitric oxide (NO) and carbon monoxide (CO) exposure on lung function in palm oil factory workers and compare it to the lung function of individuals in the surrounding community. Methods: A cross-sectional study was conducted in Kuantan Mudik District, Riau, Indonesia. One hundred palm oil factory workers with a minimum of two years of employment and 100 community members not occupationally exposed to NO and CO were recruited. Lung function was assessed using spirometry, and NO and CO levels were measured using specialized analyzers. Participants completed the European Community Respiratory Health Survey (ECRHS) questionnaire, and demographic and clinical data were collected. Statistical analyses included univariate, bivariate, and multivariate logistic regression. Results: Factory workers exhibited significantly higher levels of NO (p=0.000) and a higher prevalence of restrictive lung function (17%) compared to the community members (3%). Multivariate analysis identified NO exposure as the most significant risk factor for restrictive lung function (p=0.005). Each unit increase in NO was associated with a 1.12-fold increased risk of restrictive lung function. CO exposure, age, gender, smoking history, and the use of personal protective equipment (PPE) were not significantly associated with lung function impairment in this study. Conclusion: Occupational exposure to NO in palm oil factories is a significant risk factor for restrictive lung function impairment. Regular monitoring of NO levels and the implementation of effective control measures are crucial to protect the respiratory health of palm oil factory workers.

Comparative evaluation of 16S rRNA primer pairs in identifying nitrifying guilds in soils under long-term organic fertilization and water management

Compared with 454 sequencing technology, short-read sequencing (e.g., Illumina) technology generates sequences of high accuracy, but limited length (&amp;lt;500 bp). Such a limitation can prove that studying a target gene using a large amplicon (&amp;gt;500 bp) is challenging. The ammonia monooxygenase subunit A (amoA) gene of ammonia-oxidizing archaea (AOA), which plays a crucial part in the nitrification process, is such a gene. By providing a full overview of the community of a functional microbial guild, 16S ribosomal ribonucleic acid (rRNA) gene sequencing could overcome this problem. However, it remains unclear how 16S rRNA primer selection influences the quantification of relative abundance and the identification of community composition of nitrifiers, especially AOA. In the present study, a comparison was made between the performance of primer pairs 338F-806R, 515F-806R, and 515F-907R to a shotgun metagenome approach. The structure of nitrifier communities subjected to different long-term organic matter amendment and water management protocols was assessed. Overall, we observed higher Chao1 richness diversity of soil total bacteria by using 515F-806R compared to 338F-806R and 515F-907R, while higher Pielou’s evenness diversity was observed by using 515F-806R and 515F-907R compared to 338F-806R. The studied primer pairs revealed different performances on the relative abundance of Thaumarchaeota, AOB, and NOB. The Thaumarchaeota 16S rRNA sequence was rarely detected using 338F-806R, while the relative abundances of Thaumarchaeota detected using 515F-806R were higher than those detected by using 515F-907R. AOB showed higher proportions in the 338F-806R and 515F-907R data, than in 515F-806R data. Different primers pairs showed significant change in relative proportion of NOB. Nonetheless, we found consistent patterns of the phylotype distribution of nitrifiers in different treatments. Nitrosopumilales (NP) and Nitrososphaerales (NS) clades were the dominant members of the AOA community in soils subject to controlled irrigation, whereas Ca. Nitrosotaleales (NT) and NS clades dominated the AOA community in soils subject to flooding irrigation. Nitrospira lineage II was the dominant NOB phylotype in all samples. Overall, ideal 16S rRNA primer pairs were identified for the analysis of nitrifier communities. Moreover, NP and NT clades of AOA might have distinct environmental adaptation strategies under different irrigation treatments.

Needs, Experiences, and Hopes for Aging Futures among Older Adults in the LGBTQ Communities: A Qualitative Study in Israel.

Older lesbian, gay, bisexual, trans, and queer (LGBTQ) individuals tend to live alone, mostly without children and with scarce support from nuclear family members or biological kin. Moreover, traditional resources may not suit their specific end-of-life care needs. While studies have examined these topics in general, they lack focus on end-of-life needs, care, and planning in Israel. Moreover, research on this topic among members of LGBTQ communities is specifically lacking. This study, therefore, aimed at identifying and understanding the attitudes, perceptions, and meanings of older LGBTQ individuals in Israel regarding their needs and challenges, as they age and near end of life. The phenomenological qualitative research methodology was applied, following the interpretive approach. Twenty-one middle-aged and older LGBTQ individuals in Israel, aged ≥ 55, participated in the study. In-depth semi-structured interviews, conducted from November 2020 to April 2021, were audio-recorded, transcribed, and de-identified. Five themes emerged from the interviews: (1) Experiences of loneliness, marginalization, and trauma, and coping through liberation; (2) ageism and exclusion of older adults; (3) elastic and challenging relationships; (4) end of life as reverting into the closet and heteronormativity; and (5) death as a source of generativity and creativity. The study demonstrates that loneliness is an existential experience, exacerbated by the intersectionality of LGBTQ communities. In turn, chosen family members play a minimal role in the end-of-life care of their loved ones. While conveying ambivalence toward social services and housing for the aging, participants in this study expressed fear of being discriminated against and having to re-enter the closet as they age. Ageism and end of life do not represent finality and extinction, yet instead, signify hope and revival. Following Sandberg and Marshall's (2017) concept of queering aging futures, this study refines our understanding of life courses, demonstrating that living and thriving in old age could be positive and desirable. As such, ageism and end of life do not necessarily represent finality and extinction, and may instead signify hope and revival. The unique challenges associated with family and social support of older adults who are LGBTQ members, and their implications on care, deserve further research and are important for practice.

“Lusog-Linang”: Utilizing Community-engaged Research towards Capacity Building in Health of an Underserved Community

Background and Objectives. Nurses have a pivotal role in nurturing the capabilities of individuals, families, and population groups for better health. However, underserved communities in the Philippines, such as those with socioeconomic inadequacies, geographic isolation, and service access problems, have significant limitations in attaining the highest possible level of health. Hence, a community extension service (Project “Lusog-Linang”), employing community-engaged research, was launched with the residents of an underserved locality in Central Luzon. The current paper aimed to describe the engagement of the community residents in describing their current health situation and related priority problems, as well as explore appropriate initiatives to manage the identified problems. Methods. Community-engaged research was utilized to enable the residents of Barangay San Vicente, Bamban, Tarlac to participate in health capacity building. Particularly, records review and focus group discussions were conducted to assess the community’s health needs and to identify the residents’ perceived problems. The findings were presented in a community assembly, where residents further discussed their priority health concerns and potential interventions to address them. Quantitative data were summarized through descriptive statistics, while qualitative information was synthesized via content analysis. Results. The priority community health problems included healthcare inaccessibility (trained staff, health equipment, and facility access), food insecurity, water supply limitations, and environmental sanitation. Moreover, there was inadequate knowledge and skills among the residents in health promotion, disease prevention, and illness management. While Barangay San Vicente had limited socioeconomic resources to optimize their health capacities, the sense of community among the residents is a vital resource towards empowering them to improve their health. Conclusion. The results could be utilized as a launching pad for developing appropriate health programs for theresidents of Barangay San Vicente. Hence, the next steps in Project “Lusog-Linang” should include the identification and training of core group members toward community mobilization, and further exploring collaborative and sustainable partnerships across organizations to ensure that the community will have long-term solutions to their problems. Consequently, this project could guide public health workers in ensuring the active involvement and participation of the community members in managing their own health.

'In our culture, we come when you die': Qualitative descriptive analysis of end-of-life perspectives in a reservation-based community.

To explore (1) perspectives and attitudes of Native Americans regarding transitions from serious illness to death, and (2) awareness about hospice and palliative care service models in a Great Plains reservation-based community. Qualitative descriptive study. Community members and clinicians were invited to participate in a semi-structured focus group or interview by Tribal Advisory Board members. Analysis involved three phases: (1) qualitative descriptive analysis of preliminary themes using the Addressing Palliative Care Disparities conceptual model; (2) a cultural review of the data; and (3) reflexive thematic analysis to synthesize findings. Twenty-six participants engaged in two focus groups (n = 5-6 participants in each) and interviews (n = 15). Four themes were derived from their stories: (1) family connectedness is always priority; (2) end-of-life support is a community-wide effort; (3) everyone must grieve in their own way to heal; and (4) support needs from outside the community. Findings highlight cultural considerations spanning the life course. Clinicians, researchers and traditional wisdom keepers and practitioners, particularly those working in rural and/or reservation-based settings, must provide culturally safe care. This must include acknowledging and prioritizing the needs and preferences of Native American patients and the impact on their families and communities. Leveraging community assets, such as family and social networks, is key for supporting Native American patients with serious illnesses. Additionally, facilitating greater family and caregiver involvement along a patient's care trajectory may be a pathway for easing health care workers' caseloads in reservation-based areas, where resources are limited. The Consolidated Criteria for Reporting Qualitative Research (COREQ) guideline was used. The study was ideated based on community insight. Tribal Advisory Board members oversaw all aspects including recruitment, data acquisition, interpretation of findings and tribal data dissemination.

Emerald Ash Borer Infestation-Induced Elevated Negative Correlations and Core Genera Shift in the Endophyte Community of Fraxinus bungeana

Endophytes, prevalent in plants, mediate plant–insect interactions. Nevertheless, our understanding of the key members of endophyte communities involved in inhibiting or assisting EAB infestation remains limited. Employing ITS and 16S rRNA high-throughput sequencing, along with network analysis techniques, we conducted a comprehensive investigation into the reaction of endophytic fungi and bacteria within F. bungeana phloem by comparing EAB-infested and uninfected samples. Our findings reveal that EAB infestation significantly impacts the endophytic communities, altering both their diversity and overall structure. Interestingly, both endophytic fungi and bacteria exhibited distinct patterns in response to the infestation. For instance, in the EAB-infested phloem, the fungi abundance remained unchanged, but diversity decreased significantly. Conversely, bacterial abundance increased, without significant diversity changes. The fungi community structure altered significantly, which was not observed in bacteria. The bacterial composition in the infested phloem underwent significant changes, characterized by a substantial decrease in beneficial species abundance, whereas the fungal composition remained largely unaffected. In network analysis, the endophytes in infested phloem exhibited a modular topology, demonstrating greater complexity due to an augmented number of network nodes, elevated negative correlations, and a core genera shift compared to those observed in healthy phloem. Our findings increase understanding of plant–insect–microorganism relationships, crucial for pest control, considering endophytic roles in plant defense.

Lessons Learned Implementing Syringe Services Programs at Rural Health Departments in Kentucky.

Until recently, most syringe services programs (SSPs) in the United States operated in metropolitan areas. This study explores how SSP implementers at rural health departments in Kentucky secured support for SSP operations. In late 2020, we conducted in-depth, semi-structured interviews with 18 people involved with rural SSP implementation in Kentucky. Participants were asked to reflect on their experiences building support for SSP operations among rural health department staff and community members. Participants reported that attitudes and beliefs about SSP implementation among rural health department staff shifted quickly following engagement in educational activities and interaction with SSP clients. Participants explained that successful SSP implementation at rural health departments required sustained educational activities among community members and authorizing authorities. Future work should explore how rural communities may advocate for low-threshold and evidence-based policies that support the provision of harm reduction services.

Stakeholders’ participation in decreasing wildfire risk in the context of natural resource management in the Podpoľanie region of Slovakia

Key MessageAs the number and extent of wildfires are increasing due to climate change and human impacts, the demand for effective risk reduction strategies is growing. Due to limited resources or management capabilities in Slovakia, appropriate multistakeholder participation could help decrease the risk of wildfires while continuing to implement the principles of sustainable forest management. Thus, we recommend improving relationships with responsible stakeholders and increasing their knowledge.ContextAlthough the frequency of wildfires has declined in Slovakia, the total burned area per year and the average burned area per fire have increased rapidly, mainly due to traditional land management and increasing numbers of natural disasters resulting from climate change.AimThe objective of this study is to assess the participation and management of stakeholders in the region of Podpoľanie, which is the region of Slovakia that is most prone to wildfires. In particular, this study investigates the questions of who is accountable, in what networks, and with regard to what issues with the goal of enhancing efforts to combat wildfires.MethodsThis single-country case study features an exploratory sequential mixed-methods design. While stakeholders’ participation was explored via face-to-face interviews and interest-influence matrices, stakeholders’ prioritization was assessed via an online survey and the quantitative mapping of stakeholders’ involvement, power, interest, and knowledge.ResultsWe separately identified primary stakeholders (e.g., fire departments and state-owned forest enterprises) and secondary stakeholders (e.g., municipalities and community members). Tasks related to efforts to combat wildfires were largely in compliance with legal regulations and other mandates. Nonetheless, some stakeholders (e.g., governmental organizations involved in nature protection or nonstate forest owners and the associated enterprises) lacked the knowledge, experience, or responsibility necessary to perform these tasks.ConclusionThese stakeholders should be informed and monitored closely to achieve outcomes that can benefit a variety of stakeholders. We recommend a plan that involves improving the relationships among primary and secondary stakeholders, increasing their knowledge, and promoting effective participation to decrease the risk of wildfires in the region.