Community Function Research Articles

Using alpha, beta, and zeta diversity to map the structure and function of fish community in the central East China Sea

Using alpha, beta, and zeta diversity to map the structure and function of fish community in the central East China Sea

Environmental influence on the functional ecological structure of benthic macrofaunal communities of the northwest Iberian coast

Evaluating the functional structure of benthic macrofaunal communities provides insights into how environmental drivers shape the ecosystem and establishes a baseline knowledge of the communities’ dynamics and functioning. This understanding allows the prediction of responses to environmental changes and the implementation of efficient conservation and management strategies. Here we examine the structures and functions of benthic macrofaunal communities on the Northwest Iberian coast concerning environmental factors such as depth, hydrodynamic energy, and bottom type. The results suggest that the community assemblages and their function are structured by factors which influence food availability and habitat heterogeneity. The different sites exhibited different trait compositions and functional structures, indicating that distinct functions are performed according to environmental conditions. The communities found in sandy bottom areas with low hydrodynamic conditions presented frail functionality and demonstrated high vulnerability to alterations in their environment. Conversely, the communities found in rocky bottoms with high hydrodynamic conditions exhibited a fulfilled functional niche space, rendering them more resilient to such changes and less prone to loss of function. Although the analyses did not reveal significant differences in the factor depth, its influence on several factors seems relevant in shaping the functional structure of the communities. These findings highlight the importance of understanding the impact of local environmental conditions on ecosystem functioning, to effectively implement monitoring, management, and conservation strategies.

Nitrogen deposition in the middle-aged and mature coniferous forest: Impacts on soil microbial community structure and function

The impact of nitrogen (N) deposition on soil microbial community structure and function has been a focal point of research; however, the influence of forest age and N form on the microbial response to N deposition has yet not to be fully understood. To address this gap, metagenomic sequencing was utilized to explore the responses of soil microbial community structure and functional genes to five years of N addition in middle-aged and mature coniferous forests in southwest China. Adopting a randomized block design, the experiment included two N forms ((NH4)2SO4 and KNO3) across four levels of addition (0, 10, 20 and 40 kg N ha−1 yr−1). Our findings reveal that the composition of the microbial community structure and functional genes involved in the carbon (C), N, phosphorus (P), and sulphur (S) cycling varied significantly between middle-aged and mature forest (P < 0.001). At the phylum level, the soil microbial community in the mature forest were dominated by Proteobacteria (30.4–38.8 %), Acidobacteriota (8.5–24.1 %), and Chloroflexota (8.1–21.5 %), and the soil microbial community in the middle-aged forest showed a greater presence of Acidobacteriota (28.9–34.0 %) and Actinobacteriota (13.3–19.2 %) with a lower proportion of Chloroflexota (0.4–1.2 %). In both middle-aged and mature forests, functional gene abundance, and the composition of microbial community and functional genes were unaffected by N addition rate (P = 0.793) and N form (P = 0.725). The taxonomic composition of soil microbial community in the mature forest showed a significant positive correlation with soil pH, soil organic carbon (SOC) and total nitrogen (TN) (P < 0.01), while the microbial community composition in the middle-aged forests was not significant correlated with soil pH, SOC and TN (P > 0.05). These findings underscore that N addition in the middle-aged and mature coniferous forest does not significantly impact the soil microbial community structure and function across different N forms and N addition rate, suggesting that soil microorganisms of the forest ecosystem exhibit strong resilience to increased N supply.

Analysis of bacterial community structure, functional variation, and assembly mechanisms in multi-media habitats of lakes during the frozen period

Ice, water, and sediment represent three interconnected habitats in lake ecosystems, and bacteria are crucial for maintaining ecosystem equilibrium and elemental cycling across these habitats. However, the differential characteristics and driving mechanisms of bacterial community structures in the ice, water, and sediments of seasonally frozen lakes remain unclear. In this study, high-throughput sequencing technology was used to analyze and compare the structure, function, network characteristics, and assembly mechanisms of bacterial communities in the ice, water, and sediment of Wuliangsuhai, a typical cold region in Inner Mongolia. The results showed that the bacterial communities in the ice and water phases had similar diversity and composition, with Proteobacteria, Bacteroidota, Actinobacteria, Campilobacterota, and Cyanobacteria as dominant phyla. The bacterial communities in sediments displayed significant differences from ice and water, with Chloroflexi, Proteobacteria, Firmicutes, Desulfobacterota, and Acidobacteriota being the dominant phyla. Notably, the bacterial communities in water exhibited higher spatial variability in their distribution than those in ice and sediment. This study also revealed that during the frozen period, the bacterial community species in the ice, water, and sediment media were dominated by cooperative relationships. Community assembly was primarily influenced by stochastic processes, with dispersal limitation and drift identified as the two most significant factors within this process. However, heterogeneous selection also played a significant role in the community composition. Furthermore, functions related to nitrogen, phosphorus, sulfur, carbon, and hydrogen cycling vary among bacterial communities in ice, water, and sediment. These findings elucidate the intrinsic mechanisms driving variability in bacterial community structure and changes in water quality across different media phases (ice, water, and sediment) in cold-zone lakes during the freezing period, offering new insights for water environmental protection and ecological restoration efforts in such environments.

Metagenomic Profiling of Bacterial Communities and Functional Genes in Penaeus monodon

Penaeus monodon is one of the world’s most important aquaculture species, with its host-associated microbial community playing a crucial role in its growth, metabolism, immune response, and adaptation. In our study, we utilized Illumina high-throughput sequencing to investigate the composition, structure, and function of the intestinal microbial communities of P. monodon from two different regions in Guangdong. Our results identified 176 phyla across both populations, with Proteobacteria and Firmicutes being predominant. Furthermore, we identified 3095 genera, with Photobacterium, Vibrio, and Aliiroseovarius being the most dominant. Functional gene analysis based on KEGG data indicated that the carbohydrate metabolism and amino acid metabolism were significant at the secondary metabolic pathway level. The eggNOG functional annotation revealed that the genes involved in replication, recombination, and repair are of paramount importance. The CAZy annotation results indicated that Glycoside Hydrolases (GH) have the highest abundance. The Pfam annotation analysis showed that the two most prevalent domains are P-loop NTPase and NADP Rossmann. Our investigation provides a reference for species-level and functional-level analyses of the intestinal microbiota of P. monodon, contributing valuable insights into its microbial ecology.

Microbiomes of three coral species in the Mexican Caribbean and their shifts associated with the Stony Coral Tissue Loss Disease.

Stony Coral Tissue Loss Disease (SCTLD) has caused widespread coral mortality in the Caribbean Region. However, how the disease presence alters the microbiome community, their structure, composition, and metabolic functionality is still poorly understood. In this study, we characterized the microbial communities of the tissues of apparently healthy and diseased SCTLD colonies of the species Siderastrea siderea, Orbicella faveolata, and Montastraea cavernosa to explore putative changes related to the presence of SCTLD. Gammaproteobacteria, Alphaproteobacteria, and Bacteroidia were the best represented classes in the healthy tissues of all coral species, and alpha diversity did not show significant differences among the species. The microbial community structure between coral species was significantly different (PERMANOVA: F = 3.46, p = 0.001), and enriched genera were detected for each species: Vibrio and Photobacterium in S. siderea, Spirochaeta2 and Marivivens in O. faveolata and SAR202_clade and Nitrospira in M. cavernosa. Evidence of SCTLD in the microbial communities was more substantial in S. siderea, where differences in alpha diversity, beta diversity, and functional profiles were observed. In O. faveolata, differences were detected only in the community structure, while M. cavernosa samples showed no significant difference. Several microbial groups were found to have enriched abundances in tissue from SCTLD lesions from S. siderea and O. faveolata, but no dominant bacterial group was detected. Our results contribute to understanding microbial diversity associated with three scleractinian coral species and the shifts in their microbiomes associated with SCTLD in the Mexican Caribbean.

New insight to the enriched microorganisms driven by pollutant concentrations and types for industrial and domestic wastewater via distinguishing the municipal wastewater treatment plants

Enriched microbial communities and their metabolic function were investigated from the three wastewater treatment plants (WWTPs), which were CWWTP (coking wastewater), MWWTP1 (domestic wastewater), and MWWTP2 (mixed wastewater with domestic wastewater and effluent from various industrial WWTPs that contained the mentioned CWWTP). Pollutant types and concentrations differed among the three WWTPs and the reaction units in each WWTP. CWWTP had a higher TCN and phenol concentrations than the MWWTPs, however, in MWWTP2 no phenol was discovered but 0.72 mg/L TCN was found in its anaerobic unit. RDA results revealed that COD, TN, TP, TCN, NO3−-N, and phenol were the main factors influencing the microbial communities (P < 0.05). CPCoA confirmed the microbial community difference driven by pollutant types and concentrations (65.1% of variance, P = 0.006). They provided diverse growth environments and ecological niches for microorganisms, shaping unique bacterial community in each WWTP, as: Thiobacillus, Tepidiphilus, Soehngenia, Diaphorobacter in CWWTP; Saccharibacteria, Acidovorax, Flavobacterium, Gp4 in MWWTP1; and Mesorhizobium, Terrimicrobium, Shinella, Oscillochloris in MWWTP2. Group comparative was analyzed and indicated that these unique bacteria exhibited statistically significant difference (P < 0.01) among the WWTPs, and they were the biomarkers in each WWTP respectively. Co-occurrence and coexclusion patterns of bacteria revealed that the most of dominant bacteria in each WWTP were assigned to different modules respectively, and these microorganisms had a closer positive relationship in each module. Consistent with the functional profile prediction, xenobiotics biodegradation and metabolism were higher in CWWTP (3.86%) than other WWTPs. The distinct functional bacteria metabolized particular xenobiotics via oxidoreductases, isomerases, lyases, transferases, decarboxylase, hydroxylase, and hydrolase in each unit or WWTP. These results provided the evidences to support the idea that the pollutant types and concentration put selection stress on microorganisms in the activated sludge, shaping the distinct microbial community structure and function.

Soil depth and physicochemical properties influence microbial dynamics in the rhizosphere of two Peruvian superfood trees, cherimoya and lucuma, as shown by PacBio-HiFi sequencing

The characterization of soil microbial communities at different depths is essential to understand their impact on nutrient availability, soil fertility, plant growth and stress tolerance. We analyzed the microbial community at three depths (3 cm, 12 cm, and 30 cm) in the native fruit trees Annona cherimola (cherimoya) and Pouteria lucuma (lucuma), which provide fruits in vitamins, minerals, and antioxidants. We used PacBio-HiFi, a long-read high-throughput sequencing to explore the composition, diversity and putative functionality of rhizosphere bacterial communities at different soil depths. Bacterial diversity, encompassing various phyla, families, and genera, changed with depth. Notable differences were observed in the alpha diversity indices, especially the Shannon index. Beta diversity also varied based on plant type and depth. In cherimoya soils, positive correlations with Total Organic Carbon (TOC) and Cation Exchange Capacity (CEC) were observed, but negative ones with certain cations. In lucuma soils, indices like the Shannon index exhibited negative correlations with several metals and specific soil properties. We proposed that differences between the plant rhizosphere environments may explain the variance in their microbial diversity. This study provides insights into the microbial communities present at different soil depths, highlighting the prevalence of decomposer bacteria. Further research is necessary to elucidate their specific metabolic features and overall impact on crop growth and quality.

Unexpected methane oxidation acceleration by two species of bacteria from the rainwater promoting Methylomonas sp. in the soil

Altering the function of a mixed microbial community through specific microorganisms is a challenge. In this study, we constructed different co-culture systems to not only elucidate the mechanisms of microbial interactions but also explain the significance to the environment. Metagenomic analysis indicated that Methylomonas sp. was the main contributor to aerobic methane oxidation in the soils. Co-culture experiments showed that the introduction of Methylophilus sp. and Rhizobium sp., isolated from rainwater, significantly enhanced aerobic methane oxidation rates, on an hourly scale, representing maximum increases of 50.49 % and 24.62 %, respectively. Furthermore, metatranscriptomic analysis revealed that Methylophilus sp. and Rhizobium sp. could recolonize in the soil with abundance increasing to 1.23 % and 0.02 %. The genes of hxlA (methanol metabolism gene) and cobC (cobalamin synthesis gene) were upregulated, leading to the upregulation of methane oxidation genes such as pmoA and mmoX. The findings uncover the mechanisms of microbial interactions that improve aerobic methane oxidation and highlight the importance of these interactions in maintaining carbon cycle equilibrium in wetlands.

Characterization of Bacterial Communities on Trout Skin and Eggs in Relation to Saprolegnia parasitica Infection Status.

We have investigated the changes in the microbial communities on the surface of trout eggs and the skin of adult trout in relation to the presence of Saprolegnia parasitica. This pathogen causes saprolegniosis, a disease responsible for significant losses in salmonid farms and hatcheries. It is known from other disease systems that the host-associated microbiome plays a crucial role in the defence against pathogens, but if the pathogen predominates, this can lead to dysbiosis. However, analyses of the effects of S. parasitica on the diversity, composition, and function of microbial communities on fish skin and eggs are scarce. Thus, we have collected skin swabs from injured and healthy trout (N = 12), which differed in S. parasitica load, from three different fish farms in Croatia (Kostanjevac, Radovan, and Solin), while trout egg samples (N = 12) were infected with S. parasitica in the laboratory. Illumina sequencing of the V4 region of the 16S rRNA marker gene showed that infection with S. parasitica reduced the microbial diversity on the surface of the eggs, as evidenced by decreased Pielou's evenness and Shannon's indices. We further determined whether the bacterial genera with a relative abundance of >5.0% in the egg/skin samples were present at significantly different abundances in relation to the presence of S. parasitica. The results have shown that some genera, such as Pseudomonas and Flavobacterium, decreased significantly in the presence of the pathogen on the egg surface. On the other hand, some bacterial taxa, such as Acinetobacter and Janthinobacterium, as well as Aeromonas, were more abundant on the diseased eggs and the injured trout skin, respectively. Finally, beta diversity analyses (weighted UniFrac, unweighted UniFrac, Bray-Curtis) have shown that the sampling location (i.e., fish farm), along with S. parasitica infection status, also has a significant influence on the microbial communities' composition on the trout skin and eggs, demonstrating the strong influence of the environment on the shaping of the host surface microbiome. Overall, we have shown that the presence of S. parasitica was associated with changes in the diversity and structure of the trout skin/egg microbiome. The results obtained could support the development of new strategies for the management of saprolegniosis in aquaculture.

Pyrene biodegradation by bacterial consortia enriched from coastal and marine sediments: Insights into bacterial community structures and functions

As a representative polycyclic aromatic hydrocarbon (PAH), pyrene poses a significant threat to the environment and public health due to its persistence and potential toxicity. Extensive research has been conducted on biodegradation of pyrene, highlighting the crucial need to unravel its degradation mechanisms. Coastal and marine sediments are promising sources for discovering microbial candidates capable of degrading pyrene. In this study, three bacterial consortia, designated as BS, YS, and SS, were enriched from sediments collected from the Bohai Sea, Yellow Sea, and Liao River estuarine wetland, respectively. The bacterial consortia BS, YS, and SS demonstrated high pyrene degradation efficiencies, achieving 86.61 %, 80.39 %, and 75.70 % degradation at the concentration of 50 mg/L within 8 days, respectively. High-throughput sequencing of 16S rRNA gene amplicons revealed Pandoraea, Delftia, and Pseudomonas as the dominant populations in bacterial consortia BS, YS, and SS, respectively, with some species potentially being novel pyrene degraders. Further analysis included identifying pyrene degradation metabolites by LC-Q-TOF-MS (high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry) and predicting functional genes through PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved Stats). Based on these results, the metabolic pathways for pyrene degradation by the bacterial consortia were proposed, which predominantly involved the dioxygenation pathway. Overall, these findings should offer valuable bacterial resources for facilitating the bioremediation of PAH-contaminated environments.

Multi-habitat landscapes are more diverse and stable with improved function

Conservation, restoration and land management are increasingly implemented at landscape scales1,2. However, because species interaction data are typically habitat- and/or guild-specific, exactly how those interactions connect habitats and affect the stability and function of communities at landscape scales remains poorly understood. We combine multi-guild species interaction data (plant–pollinator and three plant–herbivore–parasitoid communities, collected from landscapes with one, two or three habitats), a field experiment and a modelling approach to show that multi-habitat landscapes support higher species and interaction evenness, more complementary species interactions and more consistent robustness to species loss. These emergent network properties drive improved pollination success in landscapes with more habitats and are not explained by simply summing component habitat webs. Linking landscape composition, through community structure, to ecosystem function, highlights mechanisms by which several contiguous habitats can support landscape-scale ecosystem services.

Difference in microbial community structure along a gradient of crater altitude: insights from the Nushan volcano.

The variation in the soil microbial community along the altitude gradient has been widely documented. However, the structure and function of the microbial communities distributed along the altitude gradient in the crater still need to be determined. We gathered soil specimens from different elevations within the Nushan volcano crater to bridge this knowledge gap. We investigated the microbial communities of bacteria and fungi in the soil. It is noteworthy that the microbial alpha diversity peaks in the middle of the crater. However, network analysis shows that bacterial (nodes 760 vs 613 vs 601) and fungal (nodes 328 vs 224 vs 400) communities are most stable at the bottom and top of the crater, respectively. Furthermore, the soil microbial network exhibited a decline, followed by an increase across varying altitudes. The core microorganisms displayed the highest correlation with pH and alkaline phosphatase (AP, as determined through redundancy analysis (RDA) and Mantel tests for correlation analysis. The fungal community has a higher number of core microorganisms, while the bacterial core microorganisms demonstrate greater susceptibility to environmental factors. In conclusion, we utilized Illumina sequencing techniques to assess the disparities in the structure and function of bacteria and fungi in the soil.IMPORTANCEThese findings serve as a foundation for future investigations on microbial communities present in volcanic soil.

Silver nanoparticles alter planktonic community structure and promote ecosystem respiration in freshwater mesocosms

The widespread use of silver nanoparticles (AgNPs) has resulted in their release into the aquatic environment, which threatens the health of aquatic ecosystems. Although the ecotoxicological effects of AgNPs have been widely reported at individual and population levels, the impact of long-term exposure to AgNPs on community structure and ecosystem function in aquatic ecosystems remains poorly understood. Herein, the present study investigated the effects of long-term exposure (28 d) to environmentally relevant concentrations (1 μg/L and 10 μg/L) of AgNPs on the community structure and function of freshwater ecosystems by artificially constructed 28 mesocosms freshwater ecosystem in experimental greenhouses, using plastic water tanks and food web manipulation. The results showed that long-term exposure to AgNPs significantly altered the community structure of zooplankton, phytoplankton, and bacterioplankton in the aquatic ecosystem. Exposure to 10 μg/L AgNPs significantly reduced the zooplankton density (70.3%, p < 0.05) and increased the phytoplankton biomass and bacterial richness and diversity via a “top-down effect.” With regards to ecosystem function, AgNPs exposure significantly increased the respiration in freshwater ecosystems but did not have a significant effect on decomposition. The partial least squares path modeling (PLS-PM) further revealed that AgNPs may have a negative impact on ecosystem functions by reducing zooplankton community density and thus increasing phytoplankton biomass. This study is the first to show that long-term exposure to environmentally relevant concentrations of AgNPs leads to alterations in plankton community structure and promotes respiration in freshwater ecosystems. It emphasizes the need for assessing the environmental risk of long-term exposure to AgNPs at the ecosystem level.

Farm dairy effluent application

Dairy intensification produces a large amount of farm dairy effluents (FDE). The final disposal of FDE is generally to the soil as fertilizer. While this use of FDE as fertilizer has numerous benefits, such as reducing the application of synthetic fertilizers or improving soil quality, there are concerns about the environmental and health risks associated with microbial pathogens from animal waste, the spread of antibiotic resistance genes, nutrient losses and greenhouse gases (GHG) emissions. Furthermore, how this agricultural practice impacts the composition and functionality of soil microbial communities has not yet been elucidated. This thesis aimed to evaluate the impact of FDE applications on soil GHG emissions, the composition, activity and diversity of its microbial community, and health risks. The FDE were collected raw and from a two-stage stabilization lagoon. The N application rate normalized to 200 kg N ha−1 was divided into four seasonal applications compared to urea fertilization or a no-aggregate control. Soil samples were taken after each application for microbial and physicochemical characterization and GHG fluxes were measured up to twenty days after each application. The pasture was harvested according to typical grazing management. Repeated application of both types of FDE increased the soil nutrient status and enhanced microbial activity. Higher fescue pasture production and macronutrient content with FDE than the control positioned FDE application to soil as an alternative disposal. However, N2O emissions increased after applying both FDE’s, most notably with raw FDE. Soil microbial community composition was not significantly influenced by FDE applications, but functional diversity shifted with raw FDE. Lagoon storage effectively reduced organic C and total N along with pathogenic indicator bacteria and changed the bacterial community composition except in winter. Beta-lactam resistance genes were detected in both FDEs, but not in the soil after repeated applications.

Effects of cortisone in zebrafish (Danio rerio): Insights into gut microbiota interactions and molecular mechanisms underlying DNA damage and apoptosis

Cortisone can enter aquatic ecosystems and pose a risk to organisms therein. However, few studies have explored the effects of cortisone on the gut microbiota of aquatic organisms. Here, we exposed zebrafish (Danio rerio) to cortisone at environmentally relevant concentrations (5.0, 50.0, or 500.0 ng L−1) for 60 days to explore its toxicological effects and their association with gut microbiota changes. The terminal deoxynucleotidyl transferase-mediated dUTP–biotin nick-end labeling assay revealed that exposure to 50 ng L−1 cortisone significantly increased the intestinal cell apoptosis rate, 8-hydroxydeoxyguanosine contents, and caspase-3 and caspase-8 activities. Moreover, the transcriptome analysis results demonstrated a notable downregulation in the expression of most differentially expressed genes associated with apoptosis pathways, as well as changes in DNA replication, oxidative stress, and drug metabolism pathways; these results indicated the occurrence of cortisone-induced stress response in zebrafish. Molecular docking analysis revealed that cortisone can bind to caspase-3 through hydrogen bonds and hydrophobic interactions but that no such interactions occur between cortisone and caspase-8. Thus, cortisone may induce oxidative DNA damage and apoptosis by activating caspase-3. Finally, the 16S rRNA sequencing results demonstrated that cortisone significantly affected microbial community structures and functions in the intestinal ecosystem. These changes may indicate gut microbiota response to cortisone-induced intestinal damage and inflammation. In conclusion, the current results clarify the mechanisms underlying intestinal response to cortisone exposure and provide a basis for evaluating the health risks of cortisone in animals.

The influence of bioturbator activity on sediment bacterial structure and function is moderated by environment

Bioturbation in coastal sediments plays a crucial role in biogeochemical cycling. However, a key knowledge gap is the extent to which bioturbation influences bacterial community diversity and ecosystem processes, such as nitrogen cycling. This study paired bacterial diversity, bioturbation activity and in situ flux measurements of oxygen and nitrogen from bioturbated sediments at six estuaries along the East coast of Australia. Bacterial community diversity, composition and predicted functional profiles were similar across burrow and surface sediments but were significantly influenced by bioturbator activity (measured as number of burrows) at sites with higher fine grain content. Sediment oxygen demand increased with bioturbator activity but changes in nitrogen cycling (as measured by fluxes and predicted bacterial functional gene analysis) were more spatially variable and were unrelated to bioturbator activity and bacterial community shifts. This study highlights how bioturbator activity influences bacterial community structure and functioning and what implications this has for biogeochemical cycles in estuarine sediments.

Structural and functional diversity of microbial communities in ancient mining sites from San Luis Potosí, México

AbstractThe purpose of this study was to evaluate the effects of potentially toxic elements (PTEs) on soil microbial community functionality and structure. Charcas and Cedral in San Luis Potosí, Mexico, are sites with ancient mining activity. This activity has generated heavy metals (HMs) polluted soils that provoke deleterious effects on microbial community. Sampling was conducted in sites with and without aged mining activity. The parameters analysed were physicochemical properties and PTE concentrations. Functional diversity was evaluated with a community‐level physiological profiling (CLPP) analysis, diversity was evaluated using the richness (S), and the indices of Shannon's diversity (H), Simpson's dominance (D) and Pielou's evenness (J) were for the structure evaluation, based on relative substrate utilisation. CLPP results of set of soils indicated the following substrate utilisation pattern: carbohydrates &gt; polymers (POL) &gt; carboxylic/ketonic acids &gt; amino acids (AmA) &gt; amine/amides (A/A). In Cedral, the AmA utilisation favoured the increase of J value. In addition, it was observed that the utilisation of these compounds decreases at a higher concentration of Al and organic matter (OM) but increases at a higher concentration of Cu, Pb, Zn, Fe, Mn, and Ni, a phenomenon opposite to that was observed in Charcas. The results of set of soil indicated that AmA utilisation increases the values of S, J, D, and H. These results indicate that the microorganisms in soil are resistant to high PTE concentrations, in that condition they need accessible carbon sources as energy and as nutrients, however, they adapted to use complex carbon sources (POL). Furthermore, it presented low OM mineralisation despite high values of H, or J, then some metabolic processes could be compromised. Therefore, an intervention for soil conservation and monitoring is required.

Long-term legacy of phytoremediation on plant succession and soil microbial communities in petroleum-contaminated sub-Arctic soils

Abstract. Phytoremediation can be a cost-effective method of restoring contaminated soils using plants and associated microorganisms. Most studies follow the impacts of phytoremediation solely across the treatment period and have not explored long-term ecological effects. In 1995, a phytoremediation study was initiated near Fairbanks, Alaska, to determine how the introduction of annual grasses and/or fertilizer would influence degradation of petroleum hydrocarbons (PHCs). After 1 year, grass and/or fertilizer-treated soils showed greater decreases in PHC concentrations compared to untreated plots. The site was then left for 15 years with no active site management. In 2011, we re-examined the site to explore the legacy of phytoremediation on contaminant disappearance, as well as on plant and soil microbial ecology. We found that the recruited vegetation and the current bulk soil microbial community structure and functioning were all heavily influenced by initial phytoremediation treatment. The number of diesel-degrading microorganisms (DDMs) was positively correlated with the percentage cover of vegetation at the site, which was influenced by initial treatment. Even 15 years later, the initial use of fertilizer had significant effects on microbial biomass, community structure, and activity. We conclude that phytoremediation treatment has long-term, legacy effects on the plant community, which, in turn, impact microbial community structure and functioning. It is therefore important to consider phytoremediation strategies that not only influence site remediation rates in the short-term but also prime the site for the restoration of vegetation over the long-term.

Functional redundancy in carrion decomposition by temperate forest ant communities

Abstract Scavenging of small avian corpses may provide ants with a valuable source of nutrients, a possibility poorly studied. Here, we assessed whether ants are efficient carrion‐decomposers of avian corpses in Mediterranean dehesas through an exclusion experiment comparing decomposition rates among three treatments: vertebrate exclusion, vertebrate + invertebrate exclusion and control. Treatments were applied in a paired design in bird cavities and on the ground in two zones to assess functional redundancy. Ant community was characterized by pitfall traps, and key functional traits influencing ants' presence on carrion were identified by using phylogenetic comparative methods. Carrion decomposition was influenced by the exclusion treatment, being significantly faster when ants and other invertebrates were not excluded, but not by location (cavity/ground). Vertebrates were less likely to detect baits in cavities and removed more bait mass in the most transformed zone. Twelve of 25 ant species in the community were found foraging on baits. Ant communities differed between carrion location and localities. Phylogenetic analyses revealed that the proneness of ant species to forage on carrion was unrelated to morphological or social traits, but behavioural, being higher among species foraging collectively. Our results show that ants play a functionally unique role in the decomposition of small corpses in dehesas, and particularly so in trees and less transformed pastureland. Decomposition involves different species in different microhabitats, suggesting high functional redundancy across ants. Our findings provide new insights into the role of ants as scavengers and expand our understanding of the functioning of Mediterranean communities.