Community Of Microorganisms Research Articles

Salinity matters the most: How environmental factors shape the diversity and structure of cyanobacterial mat communities in high altitude arid ecosystems.

Microbial mats are complex communities of benthic microorganisms that occur at the soil-water interphase in lakes' shores, streams, and ponds. In the cold, mountainous desert of Eastern Pamir (Tajikistan), where scarce water bodies are influenced by extreme environmental conditions, photosynthetic cyanobacteria form diverse mats. The mats are characterized by different morphology and thickness. Their habitats exhibit a wide range of conditions; from oligosaline to hypersaline, oligotrophic to hypertrophic, and from cold ponds to hot springs. The aim of the present study was to reveal the taxonomic composition and structure of these mats and to examine which environmental factors influence them. Fifty-one mats were collected from small water bodies around Bulunkul, Karakul, and Rangkul Lakes in 2015 and 2017. The physical and chemical properties of the water were measured in situ, while the concentration of nutrients was analyzed ex-situ. To reveal the taxonomic composition of the mats, the hypervariable V3-V4 region of the 16S rRNA gene was examined using NGS technology. The results of bioinformatic analyses were compared with microscopic observations. They showed that Cyanobacteria was the dominant phylum, constituting on average 35% of bacterial ASVs, followed by Proteobacteria (28%), Bacteroidota (11%), and Firmicutes (9%). Synechococcales, Oscillatoriales, and Nostocales orders prevailed in Oxyphotobacteria, with a low contribution of Chroococcales, Gloeobacterales, and Chroococcidiopsidales. Occasionally the non-photosynthetic Vampirivibrionia (Melainabacteria) and Sericytochromatia from sister clades to Oxyphotobacteria were noted in the samples. Moreover, there was a high percentage of unidentified cyanobacterial sequences, as well as the recently described Hillbrichtia pamiria gen. et sp. nov., present in one of the samples. Salinity, followed by Na and K concentrations, correlated positively with the composition and structure of Oxyphotobacteria on different taxonomic levels and the abundance of all bacterial ASVs. The study suggests that the investigated communities possibly host more novel and endemic species. Among the environmental factors, salinity influenced the Oxyphotobacteria communities the most. Overall, the microenvironmental factors, i.e. the conditions in each of the reservoirs seemed to have a larger impact on the diversity of microbial mats in Pamir than the "subregional" factors, related to altitude, mean annual air temperature and distance between these subregions.

Effect of Non-Nutritive Sweeteners on the Gut Microbiota.

The human gut microbiota, a complex community of microorganisms living in the digestive tract, consists of more than 1500 species distributed in more than 50 different phyla, with 99% of bacteria coming from about 30-40 species. The colon alone, which contains the largest population of the diverse human microbiota, can harbor up to 100 trillion bacteria. The gut microbiota is essential in maintaining normal gut physiology and health. Therefore, its disruption in humans is often associated with various pathological conditions. Different factors can influence the composition and function of the gut microbiota, including host genetics, age, antibiotic treatments, environment, and diet. The diet has a marked effect, impacting the gut microbiota composition, beneficially or detrimentally, by altering some bacterial species and adjusting the metabolites produced in the gut environment. With the widespread use of non-nutritive sweeteners (NNS) in the diet, recent investigations have focused on their effect on the gut microbiota as a mediator of the potential impact generated by gastrointestinal-related disturbances, such as insulin resistance, obesity, and inflammation. We summarized the results from pre-clinical and clinical studies published over the last ten years that examined the single effects of the most consumed NNS: aspartame, acesulfame-K, sucralose, and saccharin. Pre-clinical studies have given conflicting results for various reasons, including the administration method and the differences in metabolism of the same NNS among the different animal species. A dysbiotic effect of NNS was observed in some human trials, but many other randomized controlled trials reported a lack of significant impacts on gut microbiota composition. These studies differed in the number of subjects involved, their dietary habits, and their lifestyle; all factors related to the baseline composition of gut microbiota and their response to NNS. The scientific community still has no unanimous consensus on the appropriate outcomes and biomarkers that can accurately define the effects of NNS on the gut microbiota.

Paclobutrazol Biodegradation of Native Bacteria in Mango Orchards in Mekong Delta, Vietnam

Background: Paclobutrazol (PBZ), a plant growth regulator, is usually used to promote off-season flowering in mango orchards in the Mekong Delta, Vietnam. Several studies demonstrated that PBZ degradation in the soil takes several months and its residue effects to the soil environment and consequently to the plants and microorganism community. The objective of this study was to identify native bacteria that can biodegrade PBZ in the soil of mango orchards. Methods: Soil samples were collected in thirty mango orchards in Vietnam’s Mekong Delta. One gram of soil was mixed with distilled water and injected into a PBZ culture medium. After culturing, bacterial colonies were isolated and transferred onto the TSA medium in order to characterize morphological and biochemical assays and to evaluate PBZ biodegradation ability. Four strains of bacteria, which had high PBZ biodegradation, were identified and evaluated PBZ biodegradation ability. Additionally, 16S rDNA region was sequenced. Result: The results of identification indicated that the four high PBZ biodegradation bacterial strains related to Pseudomonas fluorescens, Pseudomonas nitroreducens, Burkholderia cepacia, Acinetobacter seifertii. After 30 days, the content of paclobutrazol biodegradable by these bacteria was 36.51%, 36.23%, 34.19% and 33.56%, respectively, at an initial concentration of 60 ppm.

Fungal involvement in bio- weathering of historical monument with reference to Rumi Darwazaat Lucknow (Uttar Pradesh), India

Fungi are complex communities of microorganisms that damage historic monuments. The present study was made to examine the diversity of fungi associated with deteriorated monument sites. In the present investigation, 10 samples were collected from various portions of deteriorating sites of Rumi Darwaza (gate) at Lucknow. Five fungal species isolated from deteriorated monument sites are reported in this paper. The most frequent isolated fungal species from the historic monument sites are Aspergillus niger.

Оценка динамики изменения площади связанных с водой экосистем: национальный подход

SDG indicator 6.6.1 «Change in the extent of water-related ecosystems over time» is designed to protect the integrity of ecological functions and preserve the biodiversity of water-related ecosystems - lakes, reservoirs, rivers, flooded wetlands, etc. The development of a national methodology for assessing the dynamics of changes in the area of water-related ecosystems is due to the need to generate data on the indicator within the framework of international approaches, to create a single database for making management decisions on the conservation of fresh water and the communities of plants, animals and microorganisms interacting with it.According to the concept of the international methodology, taking into account national characteristics, a project of national methodology was developed to calculate the proxy indicator 6.6.1.1 «Dynamics of changes in the area of surface water bodies», which is formed by comparing data on the area of lakes, reservoirs and rivers of the base period with the subsequent target five-year period. Based on the comparison of the base and target periods, the percentage change in spatial extent is calculated. To calculate the percentage change in spatial extent, data on the areas of lakes, reservoirs and rivers included in the Register of Surface Water Bodies of the Republic of Belarus, geoportal data on land types, certification results, cartographic calculations based on GIS, VIS and UAV own aerial photography are used. The calculation of changes in the water surface area for lakes, reservoirs, rivers was carried out. It is shown that in the study period (2018-2022), compared with the previous one (2008-2012), the water surface area of lakes (total number 2041), reservoirs (total number 84), rivers (total number 19) decreased respectively by 7,83 km2 or 0,63 %, 2,03 km2 or 0,31 %, 2,15 km2 or 0,27 %. Data, in accordance with the developed methodology, will be generated for a five-year period starting from 2023.

Remediation of Cr(VI) contaminated soil by chitosan stabilized FeS composite and the changes in microorganism community

In-suit immobilization is one of the major strategies to remediate heavy metals contaminated soil with the effectiveness largely depends on the characteristics of the added chemical reagents/materials. In this study, chitosan stabilized FeS composite (CS–FeS) was prepared to evaluate the performance of remediating the high and toxic hexavalent chromium contaminated soil from the effectiveness and microbial response aspects. The characterization analysis confirmed the successful preparation of composite, and the introduction of chitosan successfully stabilized FeS to protect it from rapid oxidation as compared to bare FeS particles. With the addition dosage at 0.1%, about 85.6% and 81.3% of Cr(VI) was reduced in 3 d based on toxicity characteristic leaching procedure (TCLP) and CaCl2 extraction, and the reduction efficiency increased to 96.6% and 94.8% in 7 d, respectively. The Cr(VI) was non-detected in the TCLP leachates with increase the CS-FeS composites to 0.5%. The percentages of HOAc-extractable Cr decreased from 25.17% to 6.12% accompanied with the increase in the residual Cr from 4.26% to 13.77% and improvement of soil enzyme activity under CS-FeS composites addition. Cr(VI) contamination reduced the diversity of microbial community in soil. Three dominate prokaryotic microorganisms, namely Proteobacteria, Actinobacteria and Firmicutes, were observed in Cr-contaminated soil. The addition of CS-FeS composites increased the microbial diversity especially for that in relative lower abundance. The relative abundance of Proteobacteria and Firmicute related to Cr-tolerance and reduction increased in CS-FeS composites added soils. Taking together, these results demonstrated the potential and promising of using the CS-FeS composites for Cr(VI) polluted soil remediation.

Negative responses of terrestrial nitrogen fixation to nitrogen addition weaken across increased soil organic carbon levels

The traditional view holds that biological nitrogen (N) fixation is energetically expensive and thus, facultative N fixers reduce N fixation rates while obligate N fixers are excluded by non-N fixers as soil N becomes rich. This view, however, contradicts the phenomenon that N fixation does not decline in many terrestrial ecosystems under N enrichment. To address this paradoxical phenomenon, we conducted a meta-analysis of N fixation and diazotroph (N-fixing microorganism) community structure in response to N addition across terrestrial ecosystems. N addition inhibited N fixation, but the inhibitory effect weakened across increased soil organic carbon (SOC) concentrations. The response ratios of N fixation (including free-living, plant-associated, and symbiotic types) to N addition were lower in the ecosystems with low SOC concentrations (<10 mg/g) than in those with medium or high SOC concentrations (10–20 and > 20 mg/g, respectively). The negative N-addition effects on diazotroph abundance and diversity also weakened across increased SOC levels. Among the climatic and soil factors, SOC was the most important predictor regarding the responses of N fixation and diazotroph community structure to N addition. Overall, our study reveals the role of SOC in affecting the responses of N fixation to N addition, which helps understand the relationships of biological N fixation and N enrichment as well as the mechanisms of terrestrial C and N coupling.

A biological aerated filter with blast furnace dust (BFD-BAF): Fe-C inner-electrolysis enhanced operation and mechanism investigation

Blast furnace dust (BFD) is a solid waste containing iron and carbon produced during iron and steel smelting, and will cause serious pollution to the environment if safe disposal cannot guaranteed. In this study, a novel filter (BFD-filler) was prepared using BFD and applied to treat domestic wastewater in biological aerated filter (BAF). The operation optimization of the reactor and the mechanism of BAF enhancement by inner-electrolysis were investigated. The results showed that the removal rates of chemical oxygen demand (COD) and NH4+-N by BFD-BAF were 95.93 % and 96.96 %. This is 26.38 % and 23.16 % higher than the conventional process and the start-up time is reduced by 13.3 %. The iron ions produced during the inner-electrolysis stimulated the growth and metabolism of microorganisms and could promote the stability of biofilm. The addition of BFD-filler affected the bacterial community of microorganisms in BAF and enhanced the bacterial abundance and specificity. Therefore, BFD-filler is suitable as a carrier for microbial attachment and immobilization and has great potential for application in BAF.

Dysbiosis of Gut Microbiome in Neurocritically Ill Patients in Intensive Care: Systematic Review

Background: The human microbiome is a community of microorganisms that live in symbiosis with humans. This relationship between humans and the microbiome affects the physiology of health and disease, but not many studies have evaluated the condition of the digestive microbiome in neurology critical condition patients and its changes. This study aims to describe the microbiome characteristics of neurological patients in the intensive care unit. Methods: We conducted a systematic literature review of the relationship between the microbiome and neurology patients admitted to the intensive care unit by searching MEDLINE/PubMed, the Cochrane Database for Systematic Reviews, and Google Scholar for articles in English published through 13 June 2022. Results: There is a change in the normal flora pattern in neurological patients who are treated in the intensive care unit. The decrease in the number of colonies occurred in commensal flora bacteria and an increase in pathogenic bacteria such as Enterobacteriaceae. Discussion: The overgrowth of opportunistic pathogens suggests dysbiosis in patients with neurocritical conditions. This is thought to be correlated with the gut-brain axis relationship. This dysbiosis evaluation can be used to provide targeted microbiome therapy and to assess the risk of clinical deterioration and death in neurologic patients in intensive care. Conclusion: This study reported a significant difference between the composition of the gut microbiota in neurology patients in intensive care and the healthy population. The magnitude of this dysbiosis increases during hospitalization in the intensive care unit and has an impact on patient outcomes. Intestinal microbiota analysis is expected to provide an overview of targeted microbial therapy and predict future patient outcomes. Keywords: Neurocritical patients, intensive care, microbiome

Vertical Distribution and Source Tracking Analysis of Bacteria Composition and Nitrogen Metabolism Function of a Typical Urban Inland River

Microorganisms play an important role in the urban river nitrogen cycle. Due to the three-dimensional fluidity of river water, it is necessary to clarify the vertical distribution of community composition and nitrogen metabolism functions of microorganisms and discover how hydrodynamic factors influence microorganism sources and community composition. Based on 16S rRNA high-throughput sequencing technology, the bacteria community composition and nitrogen metabolism function of water and sediment in the North Canal at Tongzhou District Beijing City were analyzed. The effect of environmental and hydrodynamic factors on community composition and sources were studied. The results showed that the α diversity of sediment was significantly higher than that of water. Proteobacteria was the most abundant phylum, which accounted for 54.72% and 32.36% in water and sediment, respectively. Functional prediction conducted using PICRUSt2 showed that the studied North Canal had an abundance of nitrogen metabolism ability, and 47 genes related to the nitrogen cycle were obtained. Water and sediment microorganisms had a similar distribution of nitrogen metabolism functions. The copy number of genes involved with denitrification, nitrogen assimilation, and dissimilation-reduction were high, whereas the abundance of genes related to biological nitrogen fixation and nitrification were relatively low. Source tracking analysis showed that bacteria in the water that originated from upstream, neighboring sides, and sediment were 60.05%, 37.93%, and 1.05%, respectively. The amounts of bacteria in sediment that migrated from upstream, neighboring sides, and water were 50.16%, 45.55%, and 1.55%, respectively. Environmental factors, hydrodynamic conditions, and their interactions explained water bacteria community composition for 44.22%, 3.21%, and 15.60%, respectively. For sediment bacteria, the degree of explanation was 13.05%, 1.56%, and 8.51%, respectively. This indicated that environmental factors and hydrodynamic factors controlled the community composition and nitrogen cycle functions together.

Sulfamethoxazole impact on pollutant removal and microbial community of aerobic granular sludge with filamentous bacteria

In this study, sulfamethoxazole (SMX) was employed to investigate its impact on the process of aerobic granule sludge with filamentous bacteria (FAGS). FAGS has shown great tolerance ability. FAGS in a continuous flow reactor (CFR) could keep stable with 2 μg/L of SMX addition during long-term operation. The NH4+, chemical oxygen demand (COD), and SMX removal efficiencies kept higher than 80%, 85%, and 80%, respectively. Both adsorption and biodegradation play important roles in SMX removal for FAGS. The extracellular polymeric substances (EPS) might play important role in SMX removal and FAGS tolerance to SMX. The EPS content increased from 157.84 mg/g VSS to 328.22 mg/g VSS with SMX addition. SMX has slightly affected on microorganism community. A high abundance of Rhodobacter, Gemmobacter, and Sphaerotilus of FAGS may positively correlate to SMX. The SMX addition has led to the increase in the abundance of the four sulfonamide resistance genes in FAGS.

Update on the Effect of the Urinary Microbiome on Urolithiasis.

Microbiota are ecological communities of commensal, symbiotic, and pathogenic microorganisms. The microbiome could be involved in kidney stone formation through hyperoxaluria and calcium oxalate supersaturation, biofilm formation and aggregation, and urothelial injury. Bacteria bind to calcium oxalate crystals, which causes pyelonephritis and leads to changes in nephrons to form Randall's plaque. The urinary tract microbiome, but not the gut microbiome, can be distinguished between cohorts with urinary stone disease (USD) and those without a history of the disease. In the urine microbiome, the role is known of urease-producing bacteria (Proteus mirabilis, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Providencia stuartii, Serratia marcescens, and Morganella morganii) in stone formation. Calcium oxalate crystals were generated in the presence of two uropathogenic bacteria (Escherichia coli and K. pneumoniae). Non-uropathogenic bacteria (S. aureus and Streptococcus pneumoniae) exhibit calcium oxalate lithogenic effects. The taxa Lactobacilli and Enterobacteriaceae best distinguished the healthy cohort from the USD cohort, respectively. Standardization is needed in urine microbiome research for urolithiasis. Inadequate standardization and design of urinary microbiome research on urolithiasis have hampered the generalizability of results and diminished their impact on clinical practice.

The Female Reproductive Tract Microbiome and Cancerogenesis: A Review Story of Bacteria, Hormones, and Disease

The microbiota is the complex community of microorganisms that populate a particular environment in the human body, whereas the microbiome is defined by the entire habitat-microorganisms and their environment. The most abundant and, therefore, the most studied microbiome is that of the gastrointestinal tract. However, the microbiome of the female reproductive tract is an interesting research avenue, and this article explores its role in disease development. The vagina is the reproductive organ that hosts the largest number of bacteria, with a healthy profile represented mainly by Lactobacillus spp. On the other hand, the female upper reproductive tract (uterus, Fallopian tubes, ovaries) contains only a very small number of bacteria. Previously considered sterile, recent studies have shown the presence of a small microbiota here, but there are still debates on whether this is a physiologic or pathologic occurrence. Of particular note is that estrogen levels significantly influence the composition of the microbiota of the female reproductive tract. More and more studies show a link between the microbiome of the female reproductive tract and the development of gynecological cancers. This article reviews some of these findings.

Preparation of milk-based probiotic lactic acid bacteria biofilms: A new generation of probiotics.

Biofilm is considered as a community of microorganisms in which cells adhere to each other on surfaces in a self-produced matrix of extracellular polymer compounds. In recent years, efforts to use the beneficial aspects of biofilm in probiotic research have intensified. In this study, probiotic biofilms of Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus were manufactured using milk and transferred to yogurt in whole and pulverized forms to test in real food conditions. Survival was assessed during 21 days of storage time as well as gastrointestinal conditions. The results indicated that Lp.plantarum and Lc.rhamnosus can form a very desirable and strong biofilm that can have a good protective effect on the survival of these bacteria in probiotic yogurt during processing, storage, and gastrointestinal conditions, in a way that, after 120 min of treatment in high acidic gastrointestinal conditions (pH2.0), the survival rate decreased by only 0.5 and 1.1 log CFU/ml. Probiotic biofilm can be used as a natural way of utilizing bacteria in biotechnology and fermentation, which is an excellent way to increase the utility of probiotics.

The Human Gut Virome and Its Relationship with Nontransmissible Chronic Diseases.

The human gastrointestinal tract contains large communities of microorganisms that are in constant interaction with the host, playing an essential role in the regulation of several metabolic processes. Among the gut microbial communities, the gut bacteriome has been most widely studied in recent decades. However, in recent years, there has been increasing interest in studying the influences that other microbial groups can exert on the host. Among them, the gut virome is attracting great interest because viruses can interact with the host immune system and metabolic functions; this is also the case for phages, which interact with the bacterial microbiota. The antecedents of virome-rectification-based therapies among various diseases were also investigated. In the near future, stool metagenomic investigation should include the identification of bacteria and phages, as well as their correlation networks, to better understand gut microbiota activity in metabolic disease progression.

Identification of Potential Antimicrobial Targets of Pseudomonas aeruginosa Biofilms through a Novel Screening Approach.

Pseudomonas aeruginosa is an opportunistic pathogen of considerable medical importance, owing to its pronounced antibiotic tolerance and association with cystic fibrosis and other life-threatening diseases. The aim of this study was to highlight the genes responsible for P. aeruginosa biofilm tolerance to antibiotics and thereby identify potential new targets for the development of drugs against biofilm-related infections. By developing a novel screening approach and utilizing a public P. aeruginosa transposon insertion library, several biofilm-relevant genes were identified. The Pf phage gene (PA0720) and flagellin gene (fliC) conferred biofilm-specific tolerance to gentamicin. Compared with the reference biofilms, the biofilms formed by PA0720 and fliC mutants were completely eliminated with a 4-fold-lower gentamicin concentration. Furthermore, the mreC, pprB, coxC, and PA3785 genes were demonstrated to play major roles in enhancing biofilm tolerance to gentamicin. The analysis of biofilm-relevant genes performed in this study provides important novel insights into the understanding of P. aeruginosa antibiotic tolerance, which will facilitate the detection of antibiotic resistance and the development of antibiofilm strategies against P. aeruginosa. IMPORTANCE Pseudomonas aeruginosa is an opportunistic pathogen of high medical importance and is one of the main pathogens responsible for the mortality of patients with cystic fibrosis. In addition to inherited antibiotic resistance, P. aeruginosa can form biofilms, defined as communities of microorganisms embedded in a self-produced matrix of extracellular polymeric substances adhering to each other and/or to a surface. Biofilms protect bacteria from antibiotic treatments and represent a major reason for antibiotic failure in the treatment of chronic infections caused by cystic fibrosis. Therefore, it is crucial to develop new therapeutic strategies aimed at specifically eradicating biofilms. The aim of this study was to generalize a novel screening method for biofilm research and to identify the possible genes involved in P. aeruginosa biofilm tolerance to antibiotics, both of which could improve the understanding of biofilm-related infections and allow for the identification of relevant therapeutic targets for drug development.

Bacteriostatic and Antibiofilm Efficacy of a Nisin Z Solution against Co-Cultures of Staphylococcus aureus and Pseudomonas aeruginosa from Diabetic Foot Infections

Diabetes mellitus (DM) patients frequently develop diabetic foot ulcers (DFU) which are generally infected by a community of microorganisms, mainly Staphylococcus aureus and Pseudomonas aeruginosa. These bacteria exhibit a multi-drug resistance profile and biofilm-forming ability which represent a hurdle in the treatment of diabetic foot infections (DFI). We aimed to evaluate the potential of Nisin Z, an antimicrobial peptide (AMP), as an alternative treatment for severe DFI. Nisin Z shows antibacterial activity against Gram-positive and Gram-negative bacteria and an increased antibacterial effect against Gram-negatives when added to EDTA. As such, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Biofilm Inhibitory Concentration (MBIC), and Minimum Biofilm Eradication Concentration (MBEC) were determined for Nisin Z, Nisin Z + EDTA (0.4%), and Nisin Z + EDTA incorporated into guar gum, in order to test its efficacy against S. aureus and P. aeruginosa isolated from the same DFU. Results showed that Nisin Z added to the chelation agent EDTA displayed higher antibacterial and bacteriostatic efficacy against mono and dual co-cultures of S. aureus and P. aeruginosa, and higher antibiofilm efficiency against monocultures. Nisin Z was moderately cytotoxic at 200 µg/mL. Prospect in vivo studies are needed to confirm the potential of Nisin Z supplemented with EDTA to be used as a complement to conventional antibiotic therapy for severe DFI.

Gut Microbiota and Coronary Artery Disease: Current Therapeutic Perspectives.

The human gut microbiota is the community of microorganisms living in the human gut. This microbial ecosystem contains bacteria beneficial to their host and plays important roles in human physiology, participating in energy harvest from indigestible fiber, vitamin synthesis, and regulation of the immune system, among others. Accumulating evidence suggests a possible link between compositional and metabolic aberrations of the gut microbiota and coronary artery disease in humans. Manipulating the gut microbiota through targeted interventions is an emerging field of science, aiming at reducing the risk of disease. Among the interventions with the most promising results are probiotics, prebiotics, synbiotics, and trimethylamine N-oxide (TMAO) inhibitors. Contemporary studies of probiotics have shown an improvement of inflammation and endothelial cell function, paired with attenuated extracellular matrix remodeling and TMAO production. Lactobacilli, Bifidobacteria, and Bacteroides are some of the most well studied probiotics in experimental and clinical settings. Prebiotics may also decrease inflammation and lead to reductions in blood pressure, body weight, and hyperlipidemia. Synbiotics have been associated with an improvement in glucose homeostasis and lipid abnormalities. On the contrary, no evidence yet exists on the possible benefits of postbiotic use, while the use of antibiotics is not warranted, due to potentially deleterious effects. TMAO inhibitors such as 3,3-dimethyl-1-butanol, iodomethylcholine, and fluoromethylcholine, despite still being investigated experimentally, appear to possess anti-inflammatory, antioxidant, and anti-fibrotic properties. Finally, fecal transplantation carries conflicting evidence, mandating the need for further research. In the present review we summarize the links between the gut microbiota and coronary artery disease and elaborate on the varied therapeutic measures that are being explored in this context.

Antarctic Ardley Island terrace - An ideal place to study the marine to terrestrial succession of microbial communities.

The study of chronosequences is an effective tool to study the effects of environmental changes or disturbances on microbial community structures, diversity, and the functional properties of ecosystems. Here, we conduct a chronosequence study on the Ardley Island coastal terrace of the Fildes Peninsula, Maritime Antarctica. The results revealed that prokaryotic microorganism communities changed orderly among the six successional stages. Some marine microbial groups could still be found in near-coastal soils of the late stage (lowest stratum). Animal pathogenic bacteria and stress-resistant microorganisms occurred at the greatest level with the longest succession period. The main driving factors for the succession of bacteria, archaea, and fungi along Ardley Island terrace were found through Adonis analysis (PERMANOVA). During analysis, soil elements Mg, Si, and Na were related to the bacterial and archaeal community structure discrepancies, while Al, Ti, K, and Cl were related to the fungal community structure discrepancies. On the other hand, other environmental factors also play an important role in the succession of microbial communities, which could be different among each microorganism. The succession of bacterial communities is greatly affected by pH and water content; archaeal communities are greatly affected by ; fungal communities are affected by nutrients such as . In the analysis of the characteristic microorganisms along terrace, the succession of microorganisms was found to be influenced by complex and comprehensive factors. For instance, environmental instability, relationship with plants and ecological niches, and environmental tolerance. The results found that budding reproduction and/or with filamentous appendages bacteria were enriched in the late stage, which might be connected to its tolerance to rapid changes and barren environments. In addition, the decline in ammonia oxidation capacity of Thaumarchaeota archaeade with succession and the evolution of the fungi-plant relationship throughout classes were revealed. Overall, this research improves the understanding of the effect of the marine-to-terrestrial transition of the Ardley Island terrace on microbial communities. These findings will lay the foundation for more in-depth research regarding microbial adaptations and evolutionary mechanisms throughout the marine-terrestrial transition in the future.

Building blocks of biofilms - an engaging and hands-on microbiology outreach activity for school children and the general public.

Biofilms are naturally occurring communities of micro-organisms, attached to a surface and often embedded in a matrix of self-produced polymeric substances. Biofilms are widely implicated in human infections, particularly on prostheses and medical implants. Such biofilms are difficult to eradicate, often leading to replacement of the prosthesis and resulting in a significant burden to healthcare. Here we present a fun and engaging interactive activity targeted toward primary school/early secondary school children, introducing the concept of natural and healthcare-associated biofilms, using dental plaque as an archetypal example. Dental plaque forms as a result of poor oral/dental hygiene, and develops according to a typical series of defined stages: attachment and adherence to the surface, followed by colonization and maturation of the biofilm structure, and eventually, dispersal. This activity uses dental disclosing tablets to visualize real biofilms (plaque) on the participants teeth, and uses interlocking building-blocks to represent microorganisms, where children build three-dimensional 'biofilms' of varying shapes and structural integrities. Each of the stages of development are discussed in detail, and after building the biofilms, balls of different shapes, sizes and weights can be used as 'antimicrobials' to disrupt the biofilm structure. The outcomes of the activity are to enhance knowledge and general understanding of biofilms; their ubiquitous presence in the natural environment, development, implications in healthcare, and challenges of treatment. The various 'antimicrobial' balls also provide a basis to introduce and discuss drug selection for infections, and the importance of using the correct antimicrobial for different infections to avoid development of resistance.