Triticum Aestivum Research Articles

Identification of physiological race of wheat stem rust (Puccinia graminis f.sp.tritici) and reaction of bread wheat cultivars to emerging dominant races

Identification of physiological race of wheat stem rust (Puccinia graminis f.sp.tritici) and reaction of bread wheat cultivars to emerging dominant races

Transcriptome-wide m6A methylation profile reveals its potential role underlying drought response in wheat (Triticum aestivum L.).

This study revealed the transcriptome-wide m6A methylation profile under drought stress and found that TaETC9 might regulate drought tolerance through mediating RNA methylation in wheat. Drought is one of the most destructive environmental constraints limiting crop growth and development. N6-methyladenosine (m6A) is a prevalent and important post-transcriptional modification in various eukaryotic RNA molecules, playing the crucial role in regulating drought response in plants. However, the significance of m6A in wheat (Triticum aestivum L.), particularly its involvment in drought response, remains underexplored. In this study, we investigated the transcriptome-wide m6A profile under drought stress using parallel m6A immunoprecipitation sequencing (MeRIP-seq). Totally, 4221 m6A peaks in 3733 m6A-modified genes were obtained, of which 373 methylated peaks exhibited differential expression between the control (CK) and drought-stressed treatments. These m6A loci were significantly enriched in proximity to stop codons and within the 3'-untranslated region. Integration of MeRIP-seq and RNA-seq revealed a positive correlation between m6A methylation and mRNA abundance and the genes displaying both differential methylation and expression were obtained. Finally, qRT-PCR analyses were further performed and the results found that the m6A-binding protein (TaETC9) showed significant up-regulation, while the m6A demethylase (TaALKBH10B) was significantly down-regulated under drought stress, contributing to increased m6A levels. Furthermore, the loss-of-function mutant of TaECT9 displayed significantly higher drought sensitivity compared to the wild type, highlighting its role in regulating drought tolerance. This study reported the first wheat m6A profile associated with drought stress, laying the groundwork for unraveling the potential role of RNA methylation in drought responses and enhancing stress tolerance in wheat through epigenetic approaches.

Development and identification of a novel wheat-Thinopyrum ponticum disomic substitution line DS5Ag(5D) with new genes conferring resistance to powdery mildew and leaf rust

BackgroundPowdery mildew (caused by Blumeria graminis f. sp. tritici (Bgt)) and leaf rust (caused by Puccinia triticina (Pt)) are prevalent diseases in wheat (Triticum aestivum L.) production. Thinopyrum ponticum (2n = 10x = 70, EeEeEbEbExExStStStSt) contains genes that confer high levels of resistance to these diseases.ResultsAn elite wheat-Th. ponticum disomic substitution line, DS5Ag(5D), was developed in the Bainong Aikang 58 (AK58) background. The line was assessed using genomic in situ hybridization (GISH), oligo-nucleotide probe multiplex (ONPM) fluorescence in situ hybridization (FISH), and molecular markers. Twenty eight chromosome-specific molecular markers were identified for the alien chromosome, and 22 of them were co-dominant. Additionally, SNP markers from the wheat 660 K SNP chip were utilized to confirm chromosome identification and they provide molecular tools for tagging the chromosome in concern. The substitution line demonstrated high levels of resistance to powdery mildew throughout its growth period and to leaf rust at the adult stage. Based on the resistance evaluation of five F5 populations between the substitution lines and wheat genotypes with different levels of sensitivity to the two diseases. Results showed that the resistance genes located on 5Ag confered stable resistance against both diseases across different backgrounds. Resistance spectrum analysis combined with diagnostic marker detection of known resistance genes of Th. ponticum revealed that 5Ag contained two novel genes, Pm5Ag and Lr5Ag, which conferred resistance to powdery mildew and leaf rust, respectively.ConclusionsIn this study, a novel wheat-Th. ponticum disomic substitution line DS5Ag(5D) was successfully developed. The Th. ponticum chromosome 5Ag contain new resistance genes for powdery mildew and leaf rust. Chromosomic—specific molecular markers were generated and they can be used to track the 5Ag chromosome fragments. Consequently, this study provides new elite germplasm resources and molecular markers to facilitate the breeding of wheat varieties that is resistant to powdery mildew and leaf rust.

Genome-wide association studies on resistance to powdery mildew in cultivated emmer wheat.

Powdery mildew, caused by the fungal pathogen Blumeria graminis (DC.) E. O. Speer f. sp. tritici Em. Marchal (Bgt), is a constant threat to global wheat (Triticum aestivum L.) production. Although ∼100 powdery mildew (Pm) resistance genes and alleles have been identified in wheat and its relatives, more is needed to minimize Bgt's fast evolving virulence. In tetraploid wheat (Triticum turgidum L.), wild emmer wheat [T. turgidum ssp. dicoccoides (Körn. ex Asch. & Graebn.) Thell.] accessions from Israel have contributed many Pm resistance genes. However, the diverse genetic reservoirs of cultivated emmer wheat [T. turgidum ssp. dicoccum (Schrank ex Schübl.) Thell.] have not been fully exploited. In the present study, we evaluated a diverse panel of 174 cultivated emmer accessions for their reaction to Bgt isolate OKS(14)-B-3-1 and found that 66% of accessions, particularly those of Ethiopian (30.5%) and Indian (6.3%) origins, exhibited high resistance. To determine the genetic basis of Bgt resistance in the panel, genome-wide association studies were performed using 46,383 single nucleotide polymorphisms (SNPs) from genotype-by-sequencing and 4331 SNPs from the 9K SNP Infinium array. Twenty-five significant SNP markers were identified to be associated with Bgt resistance, of which 21 SNPs are likely novel loci, whereas four possibly represent emmer derived Pm4a, Pm5a, PmG16, and Pm64. Most novel loci exhibited minor effects, whereas three novel loci on chromosome arms 2AS, 3BS, and 5AL had major effect on the phenotypic variance. This study demonstrates cultivated emmer as a rich source of powdery mildew resistance, and the resistant accessions and novel loci found herein can be utilized in wheat breeding programs to enhance Bgt resistance in wheat.

Atmospheric Cold Plasma as an Alternative to Chlorination in Soft Wheat Flour to Prepare High-Ratio Cakes

Atmospheric Cold Plasma as an Alternative to Chlorination in Soft Wheat Flour to Prepare High-Ratio Cakes

Genetic Analysis of Stripe Rust Resistance in the Chinese Wheat Cultivar Luomai 163.

Stripe or yellow rust (YR) caused by Puccinia striiformis tritici (Pst) is an important foliar disease affecting wheat production globally. Resistant varieties are the most economically and environmentally effective way to manage this disease. The common winter wheat (Triticum aestivum L.) cultivar Luomai 163 exhibited resistance to Pst races CYR32 and CYR33 at the seedling stage and showed a high level adult plant resistance in the field. To understand the genetic basis of YR resistance in this cultivar, 142 F5 recombinant inbred lines (RILs) derived from cross Apav#1 × LM163 and both parents were genotyped with the 16K SNP array and bulked segregant analysis sequencing (BSA-Seq). The analysis detected a major gene, YrLM163, at the seedling stage associated with the 1BL.1RS translocation. Additionally, three genes for resistance at the adult plant stage were detected on chromosome arms 1BL (Lr46/Yr29/Pm39/Sr58), 6BS and 6BL in Luomai 163, whereas Apav#1 contributed resistance at a QTL on 2BL. These QTL explained YR disease severity variations ranging from 6.9 to 54.8%. KASP markers KASP-2BL, KASP-6BS and KASP-6BL for three novel loci QYr.hzau-2BL, QYr.hzau-6BS and QYr.hzau-6BL were developed and validated. QYr.hzau-1BL, QYr.hzau-2BL and QYr.hzau-6BS showed varying degrees of resistance to YR when present individually or in combination based on genotype and phenotype analysis of a panel of 570 wheat accessions. Six RILs combining resistance alleles of all QTL, showing higher resistance to YR in the field than Luomai 163 with disease severities of 10.7-16.0%, are important germplasm resources for breeding programs to develop YR resistant wheat varieties with good agronomic traits.

A comparative analysis of soil organic carbon stock and soil aggregation in two crop sequences in the Rolling Pampa (Argentina)

Understanding how farming practices affect soil organic carbon (SOC) accumulation is essential for sustainable agriculture. Agronomic decisions, such as crop sequence, significantly influence various soil properties. However, the overall benefits of crop rotation on soil structure and SOC storage remain uncertain. We compared the effects of soybean (Glycine max L.) monocropping with a three-year crop rotation comprising wheat (Triticum aestivum L.)/soybean double crop, maize (Zea mays L.), and soybean on the vertical distribution of SOC up to one metre depth. Additionally, we examined carbon accumulation as particulate organic matter-carbon (POM-C), soil aggregation, and structural stability in the topsoil. Compared to monocropping, crop rotation increased SOC concentrations (19 vs. 16 gC kg soil⁻¹), stock (12 vs. 10.7 MgC ha⁻¹), and POM-C content (6 vs. 3.2 gC kg soil⁻¹) in the first 5 cm of soil. At 20–65 cm depth, rotation showed a marginal increment of SOC stock with respect to soybean monocropping. The increased surface SOC content in the rotation positively affected the POM-C content and macro-aggregation. However, soil structural stability showed a tendency to be higher in the monocropping, especially at a depth of 5–20 cm. Overall, crop rotation demonstrated potential for enhanced carbon sequestration in temperate agroecosystems within three years, despite not significantly improving soil structural stability.

The influence of different types and doses of fertilizers on the formation of the structure of the yield of soft winter wheat variety ‘KWS Emil’ and line ‘Prino’

The influence of different types and doses of fertilizers on the formation of the structure of the yield of soft winter wheat variety ‘KWS Emil’ and line ‘Prino’

Effects of Individual and Combined Applications of Seaweed Extracts and Plant Growth-Promoting Rhizobacteria (PGPR) on Plant Growth and Physio-Biochemical Properties of Soft Wheat and Faba Bean

ABSTRACT The research on carbon materials and plants primarily focuses on their use in soil, showing variable effects. However, significant gaps exist regarding their impact under stress conditions (such as drought, salinity, etc.) and their interactions with plant physiological processes. Long-term effects on plant health and ecosystems are understudied, as are their interactions with other biofertilizers like algae extracts and plant growth-promoting rhizobacteria (PGPR). The aim of the present study was to evaluate the effects of seaweed extracts and PGPR on plant growth and some physio-biochemical properties of soft wheat (Triticum aestivum L.) and faba bean (Vicia faba L.). Two concentrations of aqueous extract from two seaweeds, Fucus spiralis (F) and Ulva rigida (U), and the PGPR bacillus strain solution S48 (S48S) were used either individually or in combination. All treatments were applied as foliar sprays. The findings showed that the combined application of Fucus spiralis extract [25%] (F), Ulva rigida extract [50%] (U), along with the PGPR S48 bacillus strain solution (S48S) promoted plant growth (50.93 cm for faba bean) and root development (17.90 cm for wheat). This treatment also resulted in increased chlorophyll content (3.25 mg/g Fresh Weight (FW) in soft wheat and 3.93 mg/g FW in faba bean), proteins (14.66 µg/mg FW in soft wheat and 23.79 µg/mg FW in faba bean), amino acids (0.46 mg/g FW in soft wheat and 0.56 mg/g FW in faba bean), and total carbohydrates (529.8 µg/ml in soft wheat and 340.7 µg/ml in faba bean) as compared to controls. On the other hand, the nitrate reductase activity was enhanced by seaweed extract application in both crops. Phosphorus (P) uptake by soft wheat and faba bean was significantly improved following the application of Ulva rigida extract [50%] (U), the PGPR S48 bacillus strain solution (S48S), and the co-treatment of Fucus spiralis extract [25%] (F) with the PGPR S48 bacillus strain solution (S48S). The findings suggest that the combined application of Fucus spiralis extract (F) and Ulva rigida extract (U) with the PGPR S48 bacillus strain solution (S48S) can significantly improve plant growth and nutrient absorption in durum wheat and faba bean. This offers a sustainable alternative to chemical fertilizers, reducing pollution and promoting healthier ecosystems. Furthermore, these biological treatments enhance plant resilience to abiotic stresses, potentially increasing yields in challenging conditions. By reducing reliance on chemical fertilizers, these practices can save money for farmers and advance sustainable agriculture.

Nutritive value of indian dwarf wheat ears as feed

Interest in growing ancient wheat species and studying the possibility of their use as raw material for feed preparation has increased recently. Indian dwarf wheat (Triticum sphaerococcum Percival) is one of the promising grain crops. The aim of the study was to determine the nutritive value of ears of Indian dwarf wheat in different phases of ripeness for use as raw material in the production of feed in comparison with soft wheat and to determine the rational time frame of their harvesting for feed preparation. Harvesting was carried out by combing without threshing and separation of grain heap in different phases of maturity. The feed was prepared from the grain heap. Chemical analyses of feed samples were performed according to standard methods. The mid-wax ripeness phase is the optimum harvesting time of Indian dwarf wheat for preparation of feed from its ears. Its ears in this phase contain maximum essential amino acids and minimum cellulose. Feed prepared from ears of Indian dwarf wheat has a better quality than feed from soft wheat, as it contains 1.06–2.23 % more of the sum of essential amino acids and 1–5 % more of individual amino acids. This allows the crop to be used in feed preparation to improve protein quality. But its 4–10 % more higher cellulose content than feed from soft wheat is a disadvantage. Grain heap (ears) of Indian dwarf wheat, harvested without threshing in the phase of early wax and mid-wax ripeness, can be a source of increasing the nutritive value of feeds, as it contains more essential amino acids than soft wheat.

Lignin, extractives and structural carbohydrate characteristics of Thinopyrum intermedium biomass reveal additional valorization opportunities for dual-crop utilization.

Thinopyrum intermedium (Host) Barkworth & D.R. Dewey, or intermediate wheat grass (IWG), is being developed as the first widely-available perennial grain candidate. However, because the crop is still in development, grain yields are lower than those of traditional cereals. Utilization of its non-grain biomass (e.g. for biofuel production and as a source of fine chemicals) would increase the economic value of its cultivation. The present study provides a structural characterization of the lignin and cell wall carbohydrates in IWG biomass and qualitative profiling of biomass extractives and compares them to those of annual wheat (Triticum aestivum) biomass grown in the same location and growing season. The monosaccharide composition and ester-linked phenolic acid contents of vegetative biomass material from annual wheat and IWG were similar. IWG vegetative biomass is rich in feruloylated arabinoxylans (AX) with a very low substitution rate, whereas the AX from IWG bran have a slightly higher substitution rate. The structure of IWG lignin was investigated using both the quantitative derivatization followed by reductive cleavage method and 2D-NMR analysis, revealing an H:G:S lignin that incorporates tricin and is acylated with coumaric acid and smaller amounts of ferulates. IWG and wheat extractives contained fatty acids, various free phenolic compounds (tricin, monolignols and phenolic acids), phenolic conjugates and phytosterols. The present study provides firm support for the further exploration of T. intermedium biomass as a carbohydrate feedstock (e.g, abundant in lightly substituted AX and cellulose polymers) for biofuel production and source of high-value fine chemicals, such as tricin. © 2024 Society of Chemical Industry.

Optimizing Agronomic Management Practices for Enhanced Radiation Capture and Improved Radiation Use Efficiency in Winter Wheat

Increased aboveground biomass is contingent on enhanced photosynthetically active radiation intercepted by the canopy (IPAR), improved radiation use efficiency (RUE), or both. We investigated whether and how optimized agronomic management practices promote IPAR and RUE. Four integrated agronomic management treatments, i.e., local traditional practice (LP), improved local traditional practice (ILP), high-yield agronomic management (HY), and improved high-yield agronomic management (IHY), were compared over two wheat (Triticum aestivum L.) growing seasons. The average grain yield obtained with IHY was 96% relative to that of HY and was 7% and 23% higher than that with ILP and LP, respectively. Both HY and IHY consistently supported large values of the leaf area index and IPAR fraction, thereby increasing total IPAR. Treatment HY showed increased pre-anthesis RUE, manifested as a higher specific leaf nitrogen content and whole-plant N nutrition index at anthesis. The highest pre-anthesis aboveground biomass was obtained with HY due to the highest pre-anthesis IPAR and RUE. Along with a higher canopy apparent photosynthetic rate, IHY produced higher post-anthesis aboveground biomass due to its higher post-anthesis IPAR and RUE. Treatment IHY had a slightly lower total IPAR but a similar total RUE and harvest index, thus producing a slightly lower grain yield relative to HY. These results demonstrate that the optimized agronomic management practice used under IHY effectively enhances radiation capture and improves radiation utilization. Additionally, the net profit for IHY was higher than that for HY, ILP, and LP by 8%, 11%, and 88%, respectively. Considering the high grain yield, high RUE and high economic benefits, we recommend IHY as the agronomic management practice in the target region, although further study of improvements in pre-anthesis RUE is required.

Genetic diversity of promising accessions of spring soft wheat of russian and kazakh breeding for resistance to leaf and yellow rust

The study was carried out to characterize the resistance to leaf and yellow rust in promising spring common wheat accessions and to assess their diversity by Lr and Yr resistance genes for searching of promising genotypes. The material included 36 new varieties and lines, 23 of them of Russian and 13 of Kazakh breeding. Juvenile resistance to leaf and yellow rust was evaluated in the laboratory using test-clones and populations differing in virulence. Resistance to leaf rust at the seedling stage was characterized by 42 % of the accessions. 20 Lr genes were identified using specific PCR markers. Thatcher isogenic lines with identifiable genes served as positive controls. Highly effective genes Lr24 (3 accessions), LrAgi2 (3), partially effective genes Lr9 (4), Lr19 (6), ineffective genes Lr1 (7), Lr3 (13), Lr10 (4), Lr26 (12) and Lr34 (4), and wheat-rye translocation 1AL.1RS with genes of resistance to leaf, stem and yellow rust were identified in spring accessions. Resistant accessions carried two or more Lr genes. According to phytopathological analysis, no highly resistant samples to all used regional populations of the yellow rust pathogen were found. When using molecular markers, no samples with highly effective genes Yr5, Yr10, Yr15, Yr17, Yr24 were detected. Ineffective Lr9 and Yr18 genes were identified in 30 % and 8 % of the lines, respectively. The analysis indicates success in breeding for resistance to leaf rust and the need for advanced breeding for resistance to yellow rust with the involvement of genetically diverse donors.

Cytogenetic identification and molecular mapping for the wheat-Thinopyrum ponticum introgression line with resistance to Fusarium head blight.

Xinong 511, a new wheat-Thinopyrum ponticum variety with excellent fusarium head blight resistance, the QTLs were mapped to the wheat chromosomes 5B and 7A with named QFhb.nwafu-5B and QFhb.nwafu-7A, respectively. Novel Fusarium head blight (FHB) resistance germplasms and genes are valuable for wheat improvement and breeding efforts. Thinopyrum ponticum, a wild relative of common wheat, is a valuable germplasm of disease resistance for wheat improvement and breeding. Xinong 511 (XN511) is a high-quality wheat variety widely cultivated in the Yellow and Huai Rivers Valley of China with stable FHB-resistance. Through analysis of pedigree materials of the wheat cultivar XN511, we found that the genetic material and FHB resistance from Th. ponticum were transmitted to the introgression line, indicating that the FHB resistance in XN511 likely originates from Th. ponticum. To further explore the genetic basis of FHB resistance in XN511, QTL mapping was conducted using the RILs population of XN511 and the susceptible line Aikang 58 (AK58). Survey with makers closely-linked to Fhb1, Fhb2, Fhb4, Fhb5, and Fhb7, indicated that both XN511 and the susceptible lines do not contain these QTL. Using bulked segregant analysis RNA-seq (BSR-Seq) and newly developed allele-specific PCR (AS-PCR) markers, QTLs in XN511 were successfully located on wheat chromosomes 5B and 7A. These findings are significant for further understanding and utilizing FHB resistance genes in wheat improvement.

Assessing the Impact of Tillage Methods on Soil Moisture Content and Crop Yield in Hungary

A decline in rainfall as a source of agricultural water has affected and will continue to affect sustainable crop production globally including in Hungary. Conservation of the greatest water reservoir is important for the sustainable development of agriculture in Hungary. The objective of this study was to evaluate the effects of the different tillage methods on soil moisture content, grain yield, and root weight of wheat (Triticum aestivum) and sunflower (Helianthus annuus) under rainfed conditions. A field study was conducted at the Józsefmajor Experimental and Training Farm (JM) of the Hungarian University of Agriculture and Life Sciences near Hatvan. The experiment consisted of six tillage treatments: disking (D, 16 cm), shallow cultivation (SC, 20 cm), no-till (NT), deep cultivation (DC, 25 cm), loosening (L, 45 cm), and plowing (P, 30 cm). Soil moisture content (SMC) was measured monthly, and grain yield and root weight were measured at the end of the cropping period. Our results showed no significant difference in SMC between conservation and conventional tillage methods in 2018. However, in 2021, greater SMC was significantly conserved under NT compared to P. Regarding the sampling date, a significant increase in moisture with time was observed. A significantly lower SMC was observed on 3 June 2019 between L and D. while on the 9 September 2020, SMC significantly differed between P and all the other treatments (D, SC, NT, DC, and L). Interestingly in 2018, SMC was significantly lower at 10–20 cm depth between L and D. Notably the effect of depth on SMC was observed as moisture significantly increased with increasing depth in all tillage treatments. Root weight was greatest at DC (1.54 t ha−1) in 2018 and under L (3.89 t ha−1) in 2021. Similarly, wheat grain yield was greatest at DC (2.48 t ha−1) in 2018, while sunflower yield in 2021 was greatest at L (3.86 t ha−1). It is comprehensible that conservation tillage methods such as L and NT can increase SMC and grain yield.

Effects of different cycocel doses and irrigation levels on grain production, quality characteristics and water use in bread wheat

Cycocel (CCC) is a synthetic substance widely used as a growth retardant in grain crops. The importance of CCC is greater under different stress conditions. In this study, the effects of different CCC doses (D0 = 0, D1 = 750, D2 = 1500, and D3 = 2250 g ha−1) and different irrigation levels (I0 = 100% as control, I1 = 75%, I2 = 50%, and I3 = 25%) on some agro-morphological characteristics, yield and quality parameters, and crop water use characteristics in a semi-dwarf bread wheat variety were investigated. The internode length, number of grains, grain weight per spike, biological yield and grain yield values showed significant reductions, especially under I3, regardless of CCC applications. Grain yields varied between 2.31-6.14 and 2.28-4.43 kg ha−1 in 2019-2020 and 2020-2021, respectively. There was no significant difference in crop water consumption among different CCC doses under the same irrigation level. The effects of increasing water stress on the decrease in plant height were greater than that of increasing CCC application. Under I3 and increasing CCC applications, the spike length was significantly reduced. The protein content, sedimentation, wet gluten ratio, and gluten index values increased significantly, especially under I2 and I3. Plant yield response factors for both biological (0.85-0.90 in 2019-2020 and 0.74-0.99 in 2020-2021) and grain yields (0.77-0.90 in 2019-2020 and 0.77-0.1.12 in 2020-2021) varied between years depending on CCC applications and climatic conditions. The foliar CCC application for the purpose of increasing crop yield during drought season might not be recommendable.

Development and characterisation of novel durum wheat-H. chilense 4Hch chromosome lines as a source for resistance to Septoria tritici blotch.

The use of wild species as a source of genetic variability is a valued tool in the framework of crop breeding. Hordeum chilense Roem. et Schult is a wild barley species that can be a useful genetic donor for sustainable wheat breeding which carries genes conferring resistance to some diseases or increasing grain quality, among others. Septoria tritici blotch (STB), caused by the Zymoseptoria tritici fungus, is one of the most important wheat diseases worldwide, affecting both bread and durum wheat and having a high economic impact. Resistance to STB has been previously described in H. chilense chromosome 4Hch. In this study, we have developed introgression lines for H. chilense chromosome 4Hch in durum wheat using interspecific crosses, advanced backcrosses, and consecutive selfing strategies. Alien H. chilense chromosome segments have been reduced in size by genetic crosses between H. chilense disomic substitution lines in durum wheat and durum wheat lines carrying the Ph1 deletion. Hordeum chilense genetic introgressions were identified in the wheat background through several plant generations by fluorescence in situ hybridisation (FISH) and simple sequence repeat (SSR) markers. An STB infection analysis has also been developed to assess STB resistance to a specific H. chilense chromosome region. The development of these H. chilense introgression lines with moderate to high resistance to STB represents an important advance in the framework of durum breeding and can be a valuable tool for plant breeders.

СТРЕССОУСТОЙЧИВЫЙ, С ХОРОШИМ КОМПЛЕКСОМ ОСНОВНЫХ ХОЗЯЙСТВЕННЫХ ПРИЗНАКОВ СОРТ ПШЕНИЦЫ ОЗИМОЙ ТЕРСИНКА

A new variety of soft winter wheat, Tersinka, has been developed in the North Caucasus Federal Scientific Agrarian Center of the Stavropol Region. The objective of research is to create adaptive, stress-resistant genotypes of universal wheat with a set of economic traits for cultivation using intensive and medium-intensive technologies in the conditions of the North Caucasus, Lower Volga and Central Black Earth regions of Russia. New varieties are bred using modern methods of classical, haploid and marker selection. It is based on intraspecific, complex, stepwise hybridization and, starting from the third generation of hybrids (F3), continuous, targeted, individual selection of new genotypes is carried out based on the traits of the model of universal wheat varieties of varying intensity that we have developed. The experiment was carried out on fallow, the method of variety placement was systematic. In the last crossing, the Don 95 variety was used as the maternal form, the Verta variety served as the paternal form. The Tersinka variety belongs to the Erythrospermum variety. During the years of study (2021–2023), the new variety always reliably exceeded the Grom standard in grain yield. On average, this excess over the standard was 1.67 t/ha. According to the growing season, Tersinka belongs to mid-season varieties. The new variety has a higher productive tillering. It is distinguished by high drought and heat resistance and reliably exceeds the standard in terms of resistance to these stresses. According to flour-baking indicators, it belongs to strong wheat improvers. It is distinguished by high field resistance to biotic stressors and a complex of major diseases.

Common metabolism and transcription responses of low-cadmium-accumulative wheat (Triticum aestivum L.) cultivars sprayed with nano-selenium

Cadmium (Cd) contamination in soils threatens food security, while cultivating low-Cd-accumulative varieties, coupled with agro-nanotechnology, offers a potential solution to reduce Cd accumulation in crops. Herein, foliar application of selenium nanoparticles (SeNPs) was performed on seedlings of two low-Cd-accumulative wheat (Triticum aestivum L.) varieties grown in soil spiked with Cd at 3 mg/kg. Results showed that foliar application of SeNPs at 0.16 mg/plant (SeNPs-M) significantly decreased the Cd content in leaves of XN-979 and JM-22 by 46.4 and 40.8 %, and alleviated oxidative damage. The wheat leaves treated with SeNPs-M underwent significant metabolic and transcriptional reprogramming. On one hand, four specialized antioxidant metabolites such as L-Tyrosine, beta-N-acetylglucosamine, D-arabitol, and monolaurin in response to SeNPs in JM-22 and XN-979 is the one reason for the decrease of Cd in wheat leaves. Moreover, alleviation of stress-related kinases, hormones, and transcription factors through oxidative post-translational modification, subsequently regulates the expression of defense genes via Se-enhanced glutathione peroxidase. These findings indicate that combining low-Cd-accumulative cultivars with SeNPs spraying is an effective strategy to reduce Cd content in wheat and promote sustainable agricultural development.

Potential effect of novel endophytic nitrogen fixing diverse species of Rahnella on growth promotion of wheat (Triticum aestivum L.)

Potential effect of novel endophytic nitrogen fixing diverse species of Rahnella on growth promotion of wheat (Triticum aestivum L.)