Common Source Research Articles

Temporal dynamics, meteorological effects, secondary organic aerosol estimation, and source identification of size‐segregated carbonaceous aerosols

AbstractDuring the period 2019–2020, size‐segregated aerosol samples containing elemental and organic carbon (EC and OC) were investigated. These samples were collected weekly using an eight‐stage cascade impactor from an urban site located at Aksaray University, Aksaray. The quantification of EC and OC was carried out through a thermal‐optical transmission device. The results revealed consistent size distribution attributes of EC and OC between winter and summer. Although EC accounted for an insignificant percentage (4.4%) of particulate matter (PM) in the PM9.0–10.0 fraction during winter, a more substantial portion of OC in the same fraction (13.4%) comprised EC. Seasonal variations were distinct for EC but not significant for OC. Strong correlations between OC and EC were observed in coarse particle fractions, indicating a common source, with weaker correlations in fine particles. The highest OC/EC ratio was in the PM0.43–0.65 fraction, followed by PM2.1–3.3. The ratio of OC to EC in fine PM exceeded the threshold of 15 consistently. The observation indicates that as particle size increases, there is a noticeable decline in the OC to EC ratios. Secondary organic aerosols (SOA) accounted for 60.8% (winter) and 89.8% (summer) of OC values, emphasizing the substantial impact of SOA on Aksaray's atmosphere. Both seasons exhibited a multimodal distribution of ambient OC. In winter, the EC distribution was dominated by fine particles, with a bimodal pattern (PM1.1–2.1 and PM0.43–0.65 peaks). Common pollutant sources, including traffic emissions, road dust, biogenic emissions, and coal combustion, were identified for both seasons in coarse and fine particle fractions. These findings underscore the importance of emission control strategies targeting fine PM in Aksaray.

A scoping review on the methodological and reporting quality of scoping reviews in China

BackgroundScoping reviews have emerged as a valuable method for synthesizing emerging evidence, providing a comprehensive contextual overview, and influencing policy and practice developments. The objective of this study is to provide an overview of scoping reviews conducted in Chinese academic institutions over the last decades.MethodWe conducted a comprehensive search of nine databases and six grey literature databases for scoping reviews conducted in Chinese academic institutions. The reporting quality of the included reviews was assessed using the Preferred Reporting Items for PRISMA-ScR checklist. We performed both quantitative and qualitative analyses, examining the conduct of the scoping reviews and exploring the breadth of research topics covered. We used Chi-squared and Wilcoxon rank-sum tests to compare methodological issues and reporting quality in English and Chinese-language reviews.ResultsA total of 392 reviews published between 2013 and 2022 were included, 238 English-reported reviews and 154 Chinese-reported reviews, respectively. The primary purposes of these reviews were to map and summarize the evidence, with a particular focus on health and nursing topics. 98.7% of reviews explicitly used the term “scoping review”, and the Arksey and O’Malley framework was the most frequently cited framework. Thirty-five English-reported scoping reviews provided a protocol for scoping review. PubMed was the most common source in English-reported reviews and CNKI in Chinese-reported reviews. Reviews published in English were more likely to search the grey literature (P = 0.005), consult information specialists (P < 0.001) and conduct an updated search (P = 0.012) than those in Chinese. Reviews published in English had a significantly high score compared to those published in Chinese (16 vs. 14; P < 0.001). The reporting rates in English-reported reviews were higher than those in Chinese reviews for seven items, but lower for structured summary (P < 0.001), eligibility criteria (P < 0.001), data charting process (P = 0.009) and data items (P = 0.015).ConclusionThere has been a significant increase in the number of scoping reviews conducted in Chinese academic institutions each year since 2020. While the research topics covered are diverse, the overall reporting quality of these reviews is need to be improved. And there is a need for greater standardization in the conduct of scoping reviews in Chinese academic institutions.

Evaluation of heavy metals in different brands of chocolates marketed in Kathmandu, Nepal, and their associated health risks

Chocolates are among the sweet food items consumed by all age groups particularly children in Nepal. However, this foodstuff may be contaminated with heavy metals from the raw ingredients, production, and packaging methods, which could bring serious health issues. Therefore, this study aimed to determine cadmium (Cd), nickel (Ni), and lead (Pb) by Flame Atomic Absorption Spectrophotometer (FAAS) in a total of thirty-seven different brands of milk-based, cocoa-based, and sugar-based chocolates available in local grocery shops of Kathmandu city, Nepal and to evaluate associated health risks in children and adults using USEPA deterministic approaches. The results revealed concentrations of Cd, Ni, and Pb in the range of 0.021 – 0.585, 1.90 – 7.24, and 0.57 – 4.29 mg/kg respectively in studied chocolates and an overall mean concentration of 0.199, 4.22, and 1.94 mg/kg respectively. The observed concentrations exceeded the maximum permissible limits set by FAO/WHO (2001). A higher concentration of all studied metals was found in cocoa-based chocolate compared to milk-based and sugar-based chocolates. The positive and significant correlations (p &lt; 0.05) among Cd, Ni, and Pb in studied chocolates indicate the possibility of contamination from common sources. Similarly, the estimated ADDing values were higher for all metals in cocoa-based chocolate for both children and adults. However, children were more prone to metals exposure than adults since their dietary intake was higher than adults. Cocoa-based chocolate in this study posed a non-carcinogenic risk to both children and adults since their hazard index (HI) values exceeded the acceptable limit (&gt;1.0). In addition, Cd and Ni posed carcinogenic risks to both receptor groups through the consumption of all three categories of chocolate. Therefore, this study suggested the use of less contaminated raw materials in chocolates as well as regular monitoring of the production chain as an attempt to ensure the quality and safety of the food products.

Street view imagery: AI-based analysis method and application

Street view imagery is an emerging form of geographic big data. It presents urban visual environments from the perspective of urban residents and also contains non-visual environment of cities, such as urban human activities and socio-economic development. However, traditional digital image processing has its limitations, and the continuous development of artificial intelligence, especially computer vision and deep learning, provides strong technical support for exploring the rich semantic information in street view imagery. This paper reviews the related research on street view imagery and its artificial intelligence analysis methods and applications. It outlines the acquisition, storage, and common data sources of street view imagery. Then it introduces computer vision, deep learning, and commonly used open-source datasets in street view imagery analysis. It also detailed three aspects of AI-based street view imagery applications, namely quantification of the physical space, urban perception, and spatial semantic speculation. Finally, issues like data acquisition, domain adaption and deep learning black box are discussed. The hotspots and prospects for the development of this research topic are also prospected.

Investigating the Sources of the French Grammar (1724) by Ivan Gorlitsky

The article is dedicated to the grammar of the French language, which is kept in the manuscript department of the Library of the Academy of Sciences (RO BAN, P I B, №106). The authorship of the manual is attributed to Ivan Gorlitsky (1690–1777). It was apparently intended for Princess Elizaveta Petrovna. The article supplements the information on this source in the works by S. V. Vlasov, L. V. Moskovkin (2013, 2017), N. V. Kareva and M. G. Sharikhina (2022). The allegation passed down from work to work about the originality of Gorlitsky’s work is disputed. Evidence shows that a significant part of the grammar text corresponds to the textbook “The true principles of the French Language” (1757) by V. J. Peyton; it is assumed that both texts go back to a common source. Other fragments of Gorlitsky’s grammar go back to F. de Fenne’s «Institutio linguae gallicae» (1680). The examples are generously taken from J.-R. Peplier’s textbook (1689). Special attention is paid to the peculiarities of the material adaptation to the Russian reader. It is pointed out that Gorlitsky often altered the examples and supplemented his text with information about the Russian language.

Characterizing the diversity and commensal origins of penA mosaicism in the genus Neisseria.

Mosaic penA alleles formed through horizontal gene transfer (HGT) have been instrumental to the rising incidence of ceftriaxone-resistant gonococcal infections. Although interspecies HGT of regions of the penA gene between Neisseria gonorrhoeae and commensal Neisseria species has been described, knowledge concerning which species are the most common contributors to mosaic penA alleles is limited, with most studies examining only a small number of alleles. Here, we investigated the origins of recombinant penA alleles through in silico analyses that incorporated 1700 penA alleles from 35 513 Neisseria isolates, comprising 15 different Neisseria species. We identified Neisseria subflava and Neisseria cinerea as the most common source of recombinant sequences in N. gonorrhoeae penA. This contrasted with Neisseria meningitidis penA, for which the primary source of recombinant DNA was other meningococci, followed by Neisseria lactamica. Additionally, we described the distribution of polymorphisms implicated in antimicrobial resistance in penA, and found that these are present across the genus. These results provide insight into resistance-related changes in the penA gene across human-associated Neisseria species, illustrating the importance of genomic surveillance of not only the pathogenic Neisseria, but also of the oral niche-associated commensals from which these pathogens are sourcing key genetic variation.

“We have a lot weighing on us:” a Black Feminist analysis of U.S. newspaper quotes of Black women in year 1 of the COVID-19 pandemic

ABSTRACT This study examined Black women’s quotes in seven major U.S. newspapers during the first year of the COVID-19 pandemic. Lexis Uni and a university news database were used to locate articles from March 1, 2020, to March 31, 2021. The study sought to determine what topics Black women’s quotes most focused on and what their quotes revealed about their pandemic experiences. In total, 300 quotes from Black women about the pandemic in 119 articles were identified. Media Framing theory and Black Feminist theory were used to analyze the quotes. The first round of analysis using Media Framing revealed that health and justice topics were the most prevalent in the women’s quotes. Additionally, the frames of morality, attribution, and Strong Black Woman were most frequently used. Expert women were the most common sources. The secondary analysis using Black Feminist Theory revealed that, in the pandemic’s first year, Black women’s experiences included: identifying interlocking systems of oppression, resisting interlocking systems of oppression through self-definition, self-valuation, and activism, and struggling with Black womanhood and motherhood. This analysis reveals that while the media is a gatekeeper, Black women can use the media to increase awareness of their experiences and advocate against disparities.

Insights into polybrominated diphenyl ethers: occurrence, sources, and exposure health risk in selected solid waste impacted soils

&lt;p&gt;The Coordinated management of solid waste dumps from different anthropogenic environments is important for pollution management of immediate and adjacent ecosystems. This study took three soil profile samples from three functional zones: the rural, the suburban, and the urban solid waste dumps. The samples were analyzed for the concentration, origin, and associated risks of thirty-nine Polybrominated diphenyl ethers (Ʃ39PBDEs). Σ39PBDEs demonstrated high concentrations and ranged from 0.12 to 119 ng g−1 dw, in the order of rural &amp;gt; suburban &amp;gt; urban, and topsoil &amp;gt; subsoil &amp;gt; bottom soil. BDE-10, -11, -13, -15, -17, -99 and -119 showed relative abundance. Penta-BDE was moderately above standard and screening levels and demonstrated high and medium ecological risk in the rural and semi-urban zones. The non-carcinogenic and cancer risks highlight onsite exposure risks. PCA evaluation depicts an abundance of persistent and toxic flame retardant additives, and cluster analysis showed PBDEs were from a common source—cancer and hazard index exposure risk depicted rural &amp;gt; suburban &amp;gt; urban zone. The results suggested the continual use of banned flame retardant additives in commercial formulations. A comparison of PBDE implied that PBDEs in the suburban and urban zones could have undergone environmental processes, and the rural zone received fresh deposition. Migration and exposure to the detected -BDEs may increase the risk to humans in these zones. The results highlighted the need to establish a standard management protocol and monitor legacy pollutants in environmental and biological samples around solid waste dump sites and similar catchments.&lt;/p&gt;&#x0D;

A content-quality and optimization analysis of YouTube as a source of patient information for bipolar disorder

BackgroundThe goal of this study was to identify and evaluate the use of Arabic YouTube videos on BD as a resource for patient education. MethodsA cross-sectional evaluation of YouTube videos as a source of information for patients with BD in Arabic was performed. The study was observational and, because it did not involve human subjects, it followed the STROBE guidelines whenever possible. The quality of the videos was assessed using the validated DISCERN instrument. The search strategy involved entering the term “bipolar disorder” in the YouTube search bar, and only YouTube videos in Arabic were included. ResultsA total of 58 videos were included in this study after removing duplicates and videos unrelated to BD (Figure 1). The most common source of videos was others (38%), followed by physician (33%), educational (26%), and hospital (3%). Resources covering symptoms and prognosis were mostly in the “others” category (41%). The resources or videos that covered treatment options were mainly created by physicians (41%). However, resources or videos that included a personal story mainly belonged to the “others” category (67%). ConclusionVisual health-related instructional resources still have a significant shortage. This study highlights the poor quality of videos about serious illnesses like BD. Evaluation and promotion of the creation of visual health-related educational resources should be the primary goal of future study.

Factors Influencing Climate Information Access of the Farmers in Hiran Region, Somalia

Purpose: Like other poor states and post-conflict countries, Somalia faces significant challenges in achieving the SDGs, particularly concerning food security. Because agricultural productivity is compromised, it immediately and significantly jeopardizes the nation's food security. Adapting to climate change can maximize its benefits and lessen many of its horrendous side effects. However, Somalia does not address or cover farmers' access to climatic information, which is important when organizing policy responses. This study's goal was to assess the variables that affect farmers in Somalia's Hiran region's ability to obtain climate information.&#x0D; Methodology: The target population of this study was smallholder farmers in the Hiran region, particularly in the Baladweyn and Bulaburte districts. Both purposive and random sampling were utilized. A questionnaire was used to gather data from 222 randomly chosen smallholder farmers as part of a survey research design. Descriptive statistics and binary logistic regression were used to test the data using STATA and SPSS. The data was presented using tables and figures.&#x0D; Findings: Most farmers (78%) had access to climate information. The most common sources of information on climate crises were radio (95%), agricultural extension agents (80%), and firsthand observation (75%). Gender (p = 0.020), marital status (p = 0.036), education level (p = 0.047), farm size (p = 0.000), distance to the market (p = 0.000), and support from local and international agencies (p = 0.013) had a significant correlation with farmers access to climate information. The report advised Somalia's federal government and regional and foreign non-governmental organizations to proceed with intervention plans, focusing particularly on variables that were identified.&#x0D; Unique Contribution to Theory, Practice, and Policy: Study outcomes contribute significant information to policymakers, professionals, and the federal government of Somalia to develop policies and regulations that are relevant to the farmer's needs to adapt to the negative impacts of climate crises. It will also lead to necessary coordination among different climate actors, stakeholders, and farming communities in the region to fill any climate information gap. This will finally allow farmers to access well-timed and dependable information regarding climate disasters.

War drives forest fire risks and highlights the need for more ecologically-sound forest management in post-war Ukraine.

Since 24 February 2022, Ukraine has experienced full-scale military aggression initiated by the Russian Federation. The war has had a major negative impact on vegetation cover of war-affected regions. We explored interactions between pre-war forest management and the impacts of military activities in three of the most forested Ukrainian areas of interest (AOI), affected by the war. These were forests lying between Kharkiv and Luhansk cities (AOI 'East'), forests along the Dnipro River delta (AOI 'Kherson'), and those of the Chornobyl Exclusion Zone (AOI CEZ). We used Sentinel satellite imagery to create damaged forest cover masks for the year 2022. We mapped forests with elevated fire hazard, which was defined as a degree of exposure to the fire-supporting land use (mostly an agricultural land, a common source of ignitions in Ukraine). We evaluated the forest disturbance rate in 2022, as compared to pre-war rates. We documented significant increases in non-stand replacing disturbances (low severity fires and non-fire disturbances) for all three of the AOIs. Damaged forest cover varied among the AOIs (24,180 ± 4,715ha, or 9.3% ± 1.8% in the 'East' AOI; 7,293 ± 1,925ha, or 15.7% ± 4.1% in the 'Kherson' AOI; 7,116 ± 1,274ha, or 5.0% ± 0.9% in the CEZ AOI). Among the forests damaged in 2022, the 'Kherson' AOI will likely have the highest proportion of an area with elevated fire hazard in the coming decades, as compared to other regions (89% vs. 70% in the 'East' and CEZ AOIs respectively). Future fire risks and extensive war-related disturbance of forest cover call for forest management to develop strategies explicitly addressing these factors.

Forensic investigation of falsified antimalarials using isotope ratio mass spectrometry: a pilot investigation

We explored whether isotope ratio mass spectrometry (IRMS) is useful to investigate the origin of falsified antimalarials. Forty-four falsified and genuine antimalarial samples (artesunate, artemether-lumefantrine, dihydroartemisinin-piperaquine and sulphamethopyrazine-pyrimethamine) were analyzed in bulk for carbon (C), nitrogen (N), and oxygen (O) element concentrations and stable isotope ratios. The insoluble fraction (“starch”) was extracted from 26 samples and analyzed. Samples of known geographical origin maize, a common source of excipient starch, were used to produce a comparison dataset to predict starch source. In both an initial (n = 18) and a follow-on set of samples that contained/claimed to contain artesunate/artemether (n = 26), falsified antimalarials had a range of C concentrations less than genuine comparator antimalarials and δ13C values higher than genuine comparators. The δ13C values of falsified antimalarials suggested that C4 plant-based organic material (e.g., starch derived from maize) had been included. Using the known-origin maize samples, predictions for growth water δ18O values for the extracted “starch” ranged from − 6.10 to − 1.62‰. These findings suggest that IRMS may be a useful tool for profiling falsified antimalarials. We found that C4 ingredients were exclusively used in falsified antimalarials versus genuine antimalarials, and that it may be possible to predict potential growth water δ18O values for the starch present in falsified antimalarials.

Dermatopathologist accuracy in classifying angioinvasive fungal infections using virtual microscopy.

Due to perceived difficulty in the categorization of angioinvasive fungal infections based on histopathology, variation exists in dermatopathology reporting. This study characterized the diagnosis of angioinvasive fungal infections by light microscopy at a single academic institution over an 11-year period. Subsequently, the accuracy of blinded reclassification by virtual microscopy was measured. Seventy-six specimens with hematoxylin-eosin slides were obtained from 33 patients. The mean diagnostic accuracy of dermatopathologists in differentiating mucormycosis, hyalohyphomycosis, and phaeohyphomycosis based on blinded reclassification via virtual microscopy was 74%, with a range of 65%-91%. While there was a range in diagnostic accuracy, the highest score of 91% and the identification of common sources of error suggest that histopathologic categorization of angioinvasive fungal infections can frequently be performed. However, accurate identification is not always possible given common pitfalls in diagnosis. In addition, standardized and clinically useful reporting should be considered.

Nutrition and diet myths, knowledge and practice during pregnancy and lactation among a sample of Egyptian pregnant women: a cross-sectional study

BackgroundGlobally, the burden of maternal malnutrition remains an enormous public health problem; malnourished pregnant women are at increased risk of having low-birth-weight (LBW) infants. Several reports suggest a possible association between malnutrition among lactating mothers and the production of smaller quantities of breast milk. Many women have incorrect nutrition knowledge during pregnancy due to false beliefs derived from popular practices. Our study was conducted to assess nutritional knowledge, myths, and practices among Egyptian women during pregnancy and lactation.MethodologyA pretested 2-page interview questionnaire was used to collect data from the study participants after written informed consent was obtained from them after clarification of the study’s aim. Obstetrics and gynecology experts collected the data from pregnant females who agreed to participate in private and university hospital antenatal care clinics in Cairo, Egypt.ResultsA total of 468 pregnant females completed the interview questionnaire. The mean knowledge score was 5 ± 3, with a median score of 5 and an IQR of 3–7, and the mean holding myths score was 3 ± 2, with a median score of 2 and an IQR of 2–4. Regarding the correct answers to the knowledge questions, more than 70% of the participants correctly answered that during the first six months of life, breast milk is the only food a baby requires, and less than 20% of them correctly answered that caffeine consumption could provoke premature birth. Regarding the holding myths questions, more than half of the participants held the myth that drinking moghat and helba increases the breast milk supply. We found that the most common source of knowledge during pregnancy and lactation among the participants was family and friends’ advice (60%), followed by others (doctors, previous education in school or university) (45%).ConclusionAmong a sample of Egyptian women, more than half held at least one myth about nutrition and diet during pregnancy and breastfeeding, so health education at antenatal outpatient clinics should be directed toward those myths to correct them. Older women with sufficient family income showed significantly higher knowledge scores than others.

Erysipelothrix amsterdamensis sp. nov., associated with mortalities among endangered seabirds.

Infectious diseases threaten endangered species, particularly in small isolated populations. Seabird populations on the remote Amsterdam Island in the Indian Ocean have been in decline for the past three decades, with avian cholera caused by Pasteurella multocida proposed as the primary driver. However, Erysipelothrix species have also been sporadically detected from albatrosses on Amsterdam Island and may be contributing to some of the observed mortality. In this study, we genomically characterized 16 Erysipelothrix species isolates obtained from three Indian yellow-nosed albatross (Thalassarche carteri) chick carcasses in 2019. Histological analyses suggest that they died of bacterial septicaemia. Two isolates were sequenced using both Illumina short-read and MinION long-read approaches, which - following hybrid assembly - resulted in closed circular genomes. Mapping of Illumina reads from the remaining isolates to one of these new reference genomes revealed that all 16 isolates were closely related, with a maximum of 13 nucleotide differences distinguishing any pair of isolates. The nucleotide diversity of isolates obtained from the same or different carcasses was similar, suggesting all three chicks were likely infected from a common source. These genomes were compared with a global collection of genomes from Erysipelothrix rhusiopathiae and other species from the same genus. The isolates from albatrosses were phylogenetically distinct, sharing a most recent common ancestor with E. rhusiopathiae. Based on phylogenomic analysis and standard thresholds for average nucleotide identity and digital DNA-DNA hybridization, these isolates represent a novel Erysipelothrix species, for which we propose the name Erysipelothrix amsterdamensis sp. nov. The type strain is A18Y020dT (=CIP 112216T=DSM 115297T). The implications of this bacterium for albatross conservation will require further study.

A146 UNDERWATER EMR WITHOUT SUBMUCOSAL INJECTION FOR COLONIC ANGIODYSPLASIA

Abstract Background Angiodysplasia is a common source of overt or occult colonic bleeding. Lesions are amenable to endoscopic thermal treatment such as APC, which requires a dedicated device and carries a small risk of perforation. Underwater EMR without submucosal injection (UEMR) is safe and effective for colorectal polyps, and requires no special equipment. Aims This study explores the use of UEMR to treat colonic angiodysplasia. We endeavoured to assess the effectiveness of treatment with UEMR in patients with this unique condition and further quantify the safety and adequacy of the obtained sample to achieve diagnosis. Further analyses were undertaken to determine the cost-burden of this method compared to comparable methods of treatment. Methods Retrospective review of clinical database for patients who underwent UEMR for colonic angiodysplasia between 2021 and 2023. Indications for treatment were iron deficiency anemia (N=4), Hematochezia (N=2), and melena (N=1). Lesions were excised en bloc using standard UEMR technique without submucosal injection and soft-coagulation electrosurgical settings. Results Six patients (5 females, mean age 76 ± 10) underwent colonoscopy with UEMR for treatment of angiodysplasia. One patient with extensive lesions required two endoscopic sessions; the others were treated during a single procedure. The median number of lesions was two; range, 1 to 13). A total of 24 lesions (size 7 ± 2 mm) were treated with UEMR. All lesions were located in the proximal colon (cecum, 14; ascending colon, 10). Histology of excised specimens confirmed a diagnosis of angiodysplasia. There were no procedural complications. Overt bleeding and anemia resolved in all patients. Conclusions In this series of patients, UEMR without submucosal injection was simple, safe and effective for treatment of proximal colon angiodysplasia. Advantages of this approach over conventional therapy with APC include reduced equipment needs, lower cost and histologic diagnosis. Further study comparing UEMR to conventional thermal modalities is warranted. Funding Agencies None

Safety and quality assessment of hot-drinks vending machines in Southern Italy

Vending machines (VMs) are common and convenient sources of various food and beverage items. Due to limited available information, concerns have been raised about the hygiene and safety of products dispensed by VMs. This study aims to assess the microbiological contamination of VMs in the Campania region (Italy), by combining microbiological and molecular biology analyses. The results revealed notable microbial contamination in VMs. Total Viable Count exceeded acceptable limits in VMs components, particularly in the inner walls of beverage dispensing nozzles and in the hot-drink product. Coffee and ginseng powders exhibited comparatively lower Total Viable Count values, while chocolate displayed none. Regarding pathogens, Listeria monocytogenes and Salmonella spp. were not detected in any of the samples subjected to analysis. Bacillus cereus (B. cereus) and Staphylococcus aureus (S. aureus) were detected in various VMs components. B. cereus contamination was prevalent, exceeding 80% in some surface swabs (water intake pipe, mixer bowl, and dispensing nozzle), and coincided with a high occurrence in the hot-drink final product. S. aureus was frequently found in milk powder, dispensing nozzles, and cappuccino samples. This research offers valuable insights into VM hygiene and safety, emphasizing the importance of multifaceted approaches to address microbiological contamination and promote public health. Regulatory compliance with cleaning procedures and proactive measures, such as robust self-monitoring plans and adherence to Good Manufacturing Practices, are crucial in mitigating contamination hazards and safeguarding product quality.

Extensively drug-resistant Pseudomonas aeruginosa: clinical features and treatment with ceftazidime/avibactam and ceftolozane/tazobactam in a tertiary care university hospital center in Portugal - A cross-sectional and retrospective observational study.

Extensively drug-resistant Pseudomonas aeruginosa (XDR-PA) is a growing concern due to its increasing incidence, limited therapeutic options, limited data on the optimal treatment, and high mortality rates. The study aimed to characterize the population, the outcome and the microbiological characteristics of XDR-PA identified in a Portuguese university hospital center. All XDR-PA isolates between January 2019 and December 2021 were identified. XDR-PA was defined as resistance to piperacillin-tazobactam, third and fourth generation cephalosporins, carbapenems, aminoglycosides and fluoroquinolones. A retrospective analysis of the medical records was performed. One hundred seventy-eight individual episodes among 130 patients with XDR-PA detection were identified. The most common sources of infection were respiratory (32%) and urinary tracts (30%), although skin and soft tissue infections (18%) and primary bacteremia (14%) were also prevalent. Colonization was admitted in 64 cases. Several patients had risk factors for complicated infections, most notably immunosuppression, structural lung abnormalities, major surgery, hemodialysis or foreign intravascular or urinary devices. XDR-PA identification was more frequent in male patients with an average age of 64.3 ± 17.5 years. One non-susceptibility to colistin was reported. Only 12.4% were susceptible to aztreonam. Ceftazidime-avibactam (CZA) was susceptible in 71.5% of the tested isolates. Ceftolozane-tazobactam (C/T) was susceptible in 77.5% of the tested isolates. Antibiotic regimens with XDR-PA coverage were reserved for patients with declared infection, except to cystic fibrosis. The most frequently administered antibiotics were colistin (41 cases), CZA (39 cases), and C/T (16 cases). When combination therapy was used, CZA plus colistin was preferred. The global mortality rate among infected patients was 35.1%, significantly higher in those with hematologic malignancy (50.0%, p < 0.05), followed by the ones with bacteremia (44.4%, p < 0.05) and those medicated with colistin (39.0%, p < 0.05), especially the ones with respiratory infections (60.0%). Among patients treated with CZA or C/T, the mortality rate seemed to be lower. XDR-PA infections can be severe and difficult to treat, with a high mortality rate. Even though colistin seems to be a viable option, it is likely less safe and efficient than CZA and C/T. To the best of the authors' knowledge, this is the first description of the clinical infection characteristics and treatment of XDR-PA in Portugal.

Isolation and molecular characterization of lumpy skin disease virus from Tamil Nadu, India during the outbreaks from 2020 to 2022.

Lumpy skin disease (LSD) caused by LSD virus is a WOAH notifiable, high-impact, transboundary poxviral disease of bovines. The first official report of LSDV in India is from Odisha state during August 2019. Since then, cases have been reported from many states including Tamil Nadu, a Southern state of India. The present study deals with isolation and molecular characterization of LSDV from Tamil Nadu during the period August 2020 to July 2022. LSDV was isolated in embryonated chicken eggs (ECE) and BHK 21 cells and was characterized based on P32, RPO30, and GPCR genes. The phylogenetic analysis revealed that Tamil Nadu isolates from India are closely related to other Indian strains, Kenyan strains and strains from neighboring countries such as Bangladesh, Nepal, and Myanmar confirming the common exotic source for the transboundary spread across borders. The presence of unique signature of amino acid (aa) at specific positions (A11, T12, T34, S99, and P199) in the GPCR sequence confirmed the identity of LSDV. A twelve nucleotide (nt94-105) insertion and corresponding aa (TILS) at 30-33 position was found in GPCR sequence and characteristic amino acid proline at 98 position (P98) in the RPO30 gene sequence of our isolates was similar to strains from Bangladesh, Nepal, and Myanmar. Further, dissimilarity of our isolates from Neethling like vaccine strains confirms the circulation of virulent filed strains responsible for the outbreaks.

Muslim pilgrims’ knowledge, attitudes, and practices regarding complementary and alternative medicine (CAM); a study conducted during Hajj season

Complementary and alternative medicine (CAM) has attracted much interest, and its prevalence in both developed and developing countries has increased. During the Hajj season, millions of Muslims from many different countries travel to Makkah for the pilgrimage. In dealing with health issues during the holy season, many pilgrims prefer to self-medicate with traditional remedies instead of visiting medical practitioners, which could affect the efforts of state healthcare organizations to maintain overall public health during this mass gathering. This study aims to gauge the prevalence of CAM use during Hajj, and to assess pilgrims’ beliefs and knowledge of CAM therapies, with particular reference to products available in Makkah. A cross-sectional survey was conducted in several camps and hotels occupied by Hajj pilgrims in Makkah, during Hajj 2023. CAM modalities were used by 68.8 % of the study participants during the Hajj season. There were almost equal numbers of men (53.7 %) and women (46.3 %) participants, with 88 % of the CAM users being non-Saudi and only 12 % Saudi. The majority of the CAM users belonged to two age groups, the 31–40 year group (29.9 %) and the 41–50 year group (34.5 %). The most frequent self-practice therapies were religious prayer/rituals (30.2 %), and the most popular practitioner therapies was herbal treatments (12.3 %). The most common source of CAM-related information was family/friends (29.2 %), for improving well-being reason (25.8 %). More than half of the participants (56.8 %) strongly believed that CAM therapies have the potential to cure disease, although they were unaware of possible interactions between CAM and conventional drugs (76.7 %). More than half of the participants (57.8 %) did not disclose their CAM usage to healthcare practitioners. Half of the sample said they used CAMs during Hajj because of the common belief that therapeutic products from the holy city of Makkah, such as Zamzam water, are more effective. In conclusion, CAM therapies are commonly used by Hajj pilgrims as they are presumed to be natural and therefore safe, raising concerns about the potential risks of relying on CAM without adequate consultation with healthcare providers or awareness of potential interactions between prescription drugs and CAM treatments.