Palaemon Serratus Research Articles

Arsenic and Chromium in Sea Foods from Niger Delta of Nigeria: A Case Study of Warri, Delta State

This study determined the concentration of Arsenic and Chromium in sea foods samples from Ethiope River. The sea foods were bought from different locations along the bank of the river. Arsenic concentration ranged from 0.046 ± 0.01 to 0.083 ± 0.05 mg/kg, while the chromium concentration ranged from 0.079 ± 0.04 to 0.152 ± 0.14 mg/kg. Palaemon serratus has the highest concentration of arsenic and chromium, 152 ± 0.14 mg/kg and 0.081 ± 0.04 mg/kg respectively, while Harengula jaguana has the lowest concentration of arsenic and chromium, 0.079 ± 0.04 mg/kg and 0.046 ± 0.01 mg/kg respectively. There are various oil prospecting companies and oil and gas related industries in this area which discharge untreated waste products into Ethiope River. In view of this, there is need to determine the level of arsenic and chromium contamination of the river, since the inhabitants depend on the river for fishing and other domestic uses.

Short-term toxic effects of naphthalene and pyrene on the common prawn (Palaemon serratus) assessed by a multi-parameter laboratorial approach: mechanisms of toxicity and impairment of individual fitness

The short-term (96 h) toxic effects of two polycyclic aromatic hydrocarbons (PAHs), naphthalene (NAP) and pyrene (PYR), on the common prawn (Palaemon serratus) were investigated in laboratory bioassays, including a fitness related assay based on the post-exposure swimming velocity. Other effect criteria were biomarkers of neurotoxicity, oxidative stress and bioenergetics, and mortality. In the range of concentrations tested (NAP: 0.13–8 mg/L; PYR: 0.006–0.4 mg/L), both PAHs impaired the swimming velocity, induced oxidative stress and damage, and changed the activity of lactate dehydrogenase and isocitrate dehydrogenase. NAP also caused mortality (96 h-LC50 = 3.5 mg/L). Thus, both PAHs were able to cause toxic effects on P. serratus after a short period of exposure through the water, including the reduction of individual fitness. PYR was five folds more effective in reducing the swimming velocity of P. serratus than NAP. These findings are of interest for the marine ecological risk assessment of oil spills.

Macroinvertebrates and fishes as biomonitors of heavy metal concentration in the Seixal Bay (Tagus estuary): Which species perform better?

The present study aims to evaluate the utilization of several benthic macroinvertebrate and fish species as bioindicators of heavy metal contamination in a relatively enclosed estuarine area, the Seixal Bay (Tagus estuary, Portugal). 266 specimens of 16 taxa were captured during four sampling campaigns and the concentration of lead, chromium, nickel, copper, zinc, cobalt and cadmium were quantified in fish muscle and whole soft body of the invertebrates, as well as in suspended particulate matter in the water column and sediments. Larger predator fishes with higher mobility presented low levels of contamination, probably due to their feedings sites being located outside the bay in less contaminated areas. The species of the Gobiidae family, Pomatoschistus microps, Pomatoschistus minutus and Gobius niger presented higher values of non-metabolic elements (Pb, Ni and Cd) while Scrobicularia plana, Nereis diversicolor, Carcinus maenas and Palaemon serratus were more associated to the accumulation of Cu, Cr and Zn. Nephtys hombergii and Crangon crangon, showed intermediate concentrations of all metals. These results points out to a future possible utilization of S. plana, N. diversicolor, P. serratus and C. maenas and of Pomatoschistus species as bioindicators of heavy metal contamination in ecological quality monitoring programs.

Effects of temperature and salinity on the survival and development of larval and juvenile Palaemon serratus (Decapoda: Palaemonidae) from Irish waters

The combined effects of temperature and salinity on the survival and development of larval and juvenile Palaemon serratus from the west coast of Ireland were investigated. Survival over time was measured at thirty combinations of temperature and salinity ranging from 10–19°C and 9–34‰ in a fully factorial design. Salinity had a stronger influence than temperature on survival at all larval stages except stage V. For juveniles the main effect changed from temperature between 100 and 200 degree days to salinity between 200 and 600 degree days and temperature between 600 and 800 degree days. Estimates of time taken to 50% mortality showed that juveniles tolerated lower salinities for longer periods and exhibited optimal salinity values which were 3‰ lower than larvae, at temperatures between 10 and 15°C. Larval stage durations were found to be influenced by temperature but not salinity. Comparison with published data suggests that populations of P. serratus have adapted to local conditions of temperature and salinity. The results presented here have practical implications for fisheries assessment and management, as the incorporation of environmental effects into stock–recruitment models can improve their predictive capacity.

Localization and Properties of Cholinesterases in the Common Prawn (Palaemon serratus): a Kinetic-Histochemical Study

Due to the diversity in biochemical properties and tissue distribution of cholinesterase (ChE) enzymes, a characterization should be performed before they are used as biomarkers for monitoring pesticide contamination. In this study we investigate the distribution of ChEs and their kinetic properties in diverse tissues of the common prawn Palaemon serratus. The results concerning the histochemical localization of ChEs suggest that the highest amount of ChE activity occurs in prawn eyes, followed by the brain, gills, and digestive tract. Negligible staining was observed in the muscle. We describe the kinetic properties of ChEs in eyes, gills, and hepatopancreas, investigating their substrate preferences with different thiocholine esters and their sensitivity to inhibition with selective inhibitors. The results suggest that the studied enzymes are ChEs and not nonspecific esterases, due to their apparent affinity for choline esters, with a distinct preference for the substrate acetylthiocholine, and their high sensitivity to inhibition by eserine sulfate. P. serratus eyes can be considered the best tissue for recovery of ChE enzyme: this tissue is easy to isolate, it expresses high levels of ChE, and the enzyme shows properties of a vertebrate AChE.

Heavy and trace metal concentrations in three rockpool shrimp species (Palaemon elegans, Palaemon adspersus and Palaemon serratus) from Tenerife (Canary Islands)

Trace metal concentrations (Fe, Cu, Co, Zn, Ni and V) were investigated in three rockpool shrimp species (Palaemon elegans, Palaemon adspersus and Palaemon serratus) from six littoral sampling sites (polluted and non-polluted) of Tenerife in the Canary Islands (Central Eastern Atlantic), Spain. Sex ratio for all three species has been determined: females predominate over males in all species and significant differences in total length and cephalothorax length was detected between sexes, being females larger than males. By other side, concentrations of trace metals were determined in whole specimens. Higher mean values for every metal were observed in P. adspersus. In males, higher values were observed in P. elegans and P. serratus, whereas in P. adspersus, mean values are higher in females. Finally, mean concentrations of trace metals studied were higher, in general terms, in the two clearly polluted stations: Santa Cruz of Tenerife commercial harbour and its fishery dock dependency.

Ultrastructural Changes in Hepatopancreas of Palaemon serratus, Following Treatment with Petroleum Carcinogenic Compounds

The aim of this study was to determine the effects of Polyaromatic Hydrocarbons (PAHs) on the fine structure of the hepatopancreas of Palaemon serratus. For this purpose, shrimps were intramuscularly injected with sublethal dose (100 ng μL ) of perylene, christen and benzo (a) pyrene. Samples of -1 hepatopancreas were then taken for electron microscopic examinations (three weeks postinjection). Ultrathin sections revealed that the exposure changed the structure of hepatopancreatic epithelial cells (Rand Fcells) in perylene and christen injected specimens. However, benzo (a) pyrene treated group was less affected. Major changes observed in the hepatopancreatic cells included thickening and folding of basal lamina, distorted microvillous border, condensation and margination of heterochromatin, dilation and fragmentation of RER, well-developed lysosomal compartment and swelling mitochondria. Such ultrastructural changes could serve as a useful research tool and a general biomarker in studies of toxic stress in aquatic habitat.

Seasonal structuring of a benthic community exposed to regular hypoxic events

The Western Trough of the Lough Hyne Marine Reserve in southern Ireland features annual episodes of profound hypoxia beneath an oxy-thermocline that develops each summer. Previous work had indicated that the hypoxia caused mass mortality of the sessile benthic fauna, but information about the winter fauna, or about mobile species was lacking. Here we report on a combined remote-operated vehicle, SCUBA and baited trap study, backed by regular oxygen-temperature-depth monitoring of the Trough. Our results show that there is community of resident benthic and mobile species during the oxic winter months that disappears in the summer. Mobile fish and crustaceans avoid the hypoxic areas of the Trough, though the prawn Palaemon serratus will venture into the hypoxic zone to scavenge on baits. At the oxy-thermocline itself, burrows of benthic fauna remain evident in summer, but their inhabitants show much reduced activity or death. Regular hypoxic episodes clearly structure the benthic community of the Trough, but this is not a simple matter of alternating mass mortality and recolonization; mobile species are clearly capable of avoiding the hypoxic zone in summer, but benefit from its productivity in winter and spring.

Does mercury interact with the inhibitory effect of dichlorvos on Palaemon serratus (Crustacea: Decapoda) cholinesterase?

Mercury is one of the most hazardous metals that may contaminate estuarine ecosystems and induce toxic effects on wildlife organisms. It has been suggested that impairment of cholinesterase (ChE) activity may be involved in the resulting mercury toxicity. Following Palaemon serratus exposure to mercury chloride (HgCl 2), no effect on ChE activity was observed whatever the concentration used (to 37.5 µM) or the time of exposure (to 7 days). By contrast, following 24 h exposure to dichlorvos, an organophosphate insecticide with a well-characterised anti-ChE action, decrease of ChE activity was observed until 30 to 40% basal activity, which seems to be the minimum activity required for prawn survival. In addition, HgCl 2 does not affect dichlorvos toxicity and treatments with a mixture of both compounds can be interpreted as the sum of the two independent toxicities. Therefore, mercury and insecticide toxicities are independent and ChE activity from P. serratus eyes seems to be a reliable and sensitive biomarker for organophosphate insecticides even when organisms are simultaneously exposed to mercury.

Mudflat nekton assemblages in the Tagus estuary (Portugal): distribution and feeding patterns

Mudflats are an important component of estuaries, where they may occupy large areas and provide feeding and nursery grounds for many fish and bird species, as in the Tagus estuary. However, knowledge on their role and functioning is still expanding. Spatial and temporal dynamics of nekton assemblages in a mudflat of the Tagus estuary were evaluated to determine their role in the estuarine functioning. Feeding activity and daily rations of the most important nekton species are also analyzed. Two intertidal mudflat areas (upper and lower) were sampled, as well as the adjoining subtidal area. Sampling was conducted every two months, during a yearly cycle. A 24 h sampling cycle was conducted to investigate daily feeding patterns and determine daily rations. Crangon crangon, Carcinus maenas, Palaemon longirostris, Palaemon serratus and Pomatoschistus microps dominated the nekton community. A marked seasonal pattern was noticed with the highest densities recorded in spring and summer, corresponding to recruitment episodes of these species. Highest biomass values were recorded in the subtidal zone, while the highest densities were recorded in the intertidal zone. The mudflats seem to play an important role in the dynamics of the Tagus estuary, acting as a feeding and nursery areas for several nektonic species.

Shoal and prey patch choice by co-occurring fishes and prawns: inter-taxa use of socially transmitted cues

Animals can use socially transmitted information to learn about the distribution and quality of resources without incurring the costs associated with having to search for and sample them first hand. Recently, it has been shown that the use of chemical social information specific to patterns of diet and habitat use is an important mechanism underpinning recognition and social organization in shoaling fishes. In this study we revealed that the use of resource-specific chemical information is not limited to conspecifics, or even members of the same taxon. In a series of laboratory experiments, we showed that threespine sticklebacks (Gasterosteus aculeatus) could recognize similar patterns of habitat use in common prawns (Leander serratus), preferentially orientating towards groups of prawns exposed to the same habitats as themselves, and even selecting foraging patches located close to them. Prawns were seen to use habitat-specific cues generated by conspecifics, but not by sticklebacks, suggesting that the benefits of forming these heterospecific social association patterns may be unequal for prawns and fishes. Our findings suggest that some species might use co-occurring, unrelated species as information centres in order to orient and locate resources within their surroundings.

NMR structure of mussel mytilin, and antiviral–antibacterial activities of derived synthetic peptides

Mytilin is a 34-residue antibacterial peptide from the mussel Mytilus galloprovincialis, which in addition possesses in vitro antiviral activity. The three-dimensional solution structure of the synthetic mytilin was established by using 1H NMR and consists of the common cysteine-stabilized αβ motif close to the one observed in the mussel defensin MGD-1. Mytilin is characterized by 8 cysteines engaged in four disulfide bonds (2–27, 6–29, 10–31, and 15–34) only involving the β-strand II. Hydrophilic and hydrophobic areas of mytilin account for 63% and 37%, respectively, a ratio very close to that of MGD-1 (64% and 36%). One linear and three cyclic fragments were designed from the interstrand loop sequence known to retain the biological activities in MGD-1. Only the fragment of 10 amino acids (C10C) constrained by two disulfide bonds in a stable β-hairpin structure was able to inhibit the mortality of Palaemon serratus shrimp injected with white spot syndrome virus (WSSV). Fifty percent inhibition was obtained by in vitro pre-incubation of WSSV with 45 μM of C10C compared with 7 μM for mytilin. Interaction between the fragment and the virus occurred very rapidly as 40% survival was recorded after only 1 min of pre-incubation. In addition, C10C was capable of inhibiting in vitro growth of Vibrio splendidus LGP32 (MIC 125 μM), Vibrio anguillarum (MIC 2 mM), Micrococcus lysodeikticus and Escherichia coli (MIC 1 mM). Destroying the cysteine-stabilized αβ structure or shortening the C1OC fragment to the C6C fragment with only one disulfide bond resulted in loss of both antiviral and antibacterial activities. Increasing the positive net charge did not enforce the antibacterial activity and completely suppressed the antiviral one. The C10C-designed peptide from mytilin appeared comparable in composition and structure with protegrin, tachyplesin and polyphemusin.

Accumulations of total metal in dominant shrimp species (Palaemon adspersus, Palaemon serratus, Parapenaeus longirostris) and bottom surface sediments obtained from the Northern Inner Shelf of the Sea of Marmara

The aim of this study is to determine the total metal (aluminum, copper, manganese, nickel, lead, zinc, cadmium, iron, mercury) contents of dominant shrimp species and sediments present at 1-50 m depths of the Northern Inner Shelf of the Sea of Marmara. Shrimp and sediment samples were collected from four regions (Büyükçekmece, Silivri, Tekirdağ, Sarköy) and from different depths (1, 5, 10, 20, 30, 50 m) at each region in September and November of 2003. Three shrimp species [Palaemon adspersus (Rathke, 1937), Palaemon serratus (Pennant, 1777), Parapenaeus longirostris (H. Lucas, 1846)] were identified to be dominant as a result of the examination on the obtained samples. Heavy metal contents of these three species were determined and the results were compared with the acceptable treshold values of the Seafood Standards and also with available literatures. The Cu contents were found to be higher than the treshold limits in all samples except P. longirostris from Silivri, also the Cd contents in all samples except P. adspersus from Büyükçekmece, the Zn contents only in P. adspersus and P. longirostris from Tekirdağ, and the Pb contents in all species from all regions. These high values are the indicator of industrial pollution. There is not any data in the Seafood Standards about Mn, Ni, Fe and Al contents in shrimp species. The values of these metal contents were given in the present study. The examination of total metal distributions in bottom surface sediment samples in the Northern Inner Shelf of the Sea of Marmara showed that the determined values were higher than the shale average at some depths of examined stations.

Histological changes in the ovary of Palaemon serratus (Crustacea, Decapoda) from the North Adriatic Sea

Summary Despite its considerable commercial importance, the ovarian maturation of the prawn Palaemon serratus (Crustacea, Decapoda) has been scarcely studied, especially in the marine habitats of Italian waters. For the purpose of the present study female samples were collected in the North Adriatic Sea for one year. Gonadal tissue sections were prepared using current histological techniques. The ovaries feature a “proliferative centre” from which oocytes maturing in a centrifugal direction branch out. Oocyte (n = 554) size in μm was determined at various stages of development using a semiautomatic computerized imaging scanner. Five cohorts at around 60, 120, 180, 240, 700 μm of diameter were recorded in oocyte distribution frequency during ovary maturation (spring-summer). Only two cohorts at around 60 and 120 μm were observed in oocyte distribution frequency in the immature ovary (autumn-winter). The goal of this study was to provide a preliminary characterisation of the histological structure of the ovary throughout the reproductive cycle. Although P. serratus is a commercially valuable natural resource and has potential for artificial culture, little is known as to the reproductive biology of this species and, unless remedied, this could hinder its successful rearing.

INDICATORS OF PERFORMANCE IN THE FISHERY FOR SHRIMP PALAEMON SERRATUS (PENNANT) IN IRISH COASTAL WATERS

A commercial fishery for shrimp Palaemon serratus occurs on the south and west coasts of Ireland. Two temporal phases were identified: expansion from the mid 1970s to the late 1980s when maximum national landings were less than 150 t annually; and a second phase since 1990 when most years yielded more than 200 t. Maximum landings of 548 tons were reached in 1999. Three indicators of fishery performance are examined: total annual landings, weight landed per vessel per day and the average weights of individual shrimp in October and December, reconstructed from processors' records of graded shrimp. Total landings and daily landings weights correlated positively, whereas both correlated negatively with average individual shrimp weight in October and December over a 13-year period. Periodic and possibly cyclical recruitment events are identified as a contributor to improved yield.

Feeding ecology and trophic relationships of fish species in the lower Guadiana River Estuary and Castro Marim e Vila Real de Santo António Salt Marsh

In this study we analyze the feeding ecology and trophic relationships of some of the main fish species (Soleidae, Moronidae, Mullidae, Sparidae, Mugilidae, and Batrachoididae) of the lower Estuary of the Guadiana River and the Castro Marim e Vila Real de Santo António Salt Marsh. We examined the stomachs of 1415 fish caught monthly between September 2000 and August 2001. Feeding indices and coefficients were determined and used along with the results of multivariate analysis to develop diagrams of trophic interactions (food webs). Results show that these species are largely opportunistic predators. The most important prey items are amphipods, gobies (Gobiidae), shrimps ( Palaemon serratus and Crangon crangon), and polychaete worms. The lower Estuary and associated salt marshes are important nurseries and feeding grounds for the species studied. In this area, it is therefore important to monitor the effects of changes in river runoff, nutrient input, and temperature that result from construction of the Alqueva Dam upstream.

The relationship between body size and evoked potentials from the statocysts of the prawnPalaemon serratus

The organisation of the statocyst hair cells and auditory evoked potentials (AEPs) generated by the afferents in three body size classes of prawn (Palaemon serratus) have been studied using a combination of anatomical, electron microscopic and electrophysiological approaches. The statistical examination of the relationship between the sensory setae and body size showed an increase in both the length and number of statocyst hair cells as the animal grows. In view of this finding, the response of the statocyst organ to a 500 Hz tone burst was recorded from four specimens from each size class using two subcutaneous electrodes, positioned in the carapace close to the supraoesophageal ganglion and statocyst. Neither body size nor the number of afferents in the statocyst has any significant impact on the amplitude of AEPs in response to the 500 Hz tone burst. The findings of this study show that P. serratus is capable of hearing a 500 Hz tone regardless of body size, a finding that is of ecological importance when considering the effect of anthropogenic sound on crustaceans.

Influence of biological and ecological factors on the bioaccumulation of polybrominated diphenyl ethers in aquatic food webs from French estuaries

Previous studies have shown the worldwide presence of six congeners of polybrominated diphenyl ethers (PBDEs) in marine biota (BDE-28, -47, -99, -100, -153 and -154). The objective of the present study was to document their presence, their level and their transfer in the food web of two major estuaries in France, the Loire and the Seine. PBDEs were quantified in eight principal species from the Loire, representing primary consumers (the bivalve Scrobicularia plana), omnivores (the worm Nereis diversicolor, the shrimps Crangon crangon, Palaemon elegans and Palaemon serratus, the flatfish Platichthys flesus and Solea solea) and supercarnivores (the eel Anguilla anguilla). In the Seine, only worms, bivalves, sole and eels have been studied. Parameters, which can interfere with the interpretation of contamination data (organ distribution, influence of weight or size of specimens, lipid richness, intrinsic variability), have been examined. BDE-47 was the predominant congener in all biota. Higher contamination was observed in most of the species collected from the Seine, in agreement with the higher human presence and economic activity in the Seine than in the Loire basin. PBDEs have been shown to biomagnify in both of the studied estuarine food webs. However, assessment of PBDE transfer from seafood products exposed to contaminants in the Seine estuary showed that human daily intake is far below the no observed adverse effect levels.

Cholinesterase from the common prawn ( Palaemon serratus) eyes: Catalytic properties and sensitivity to organophosphate and carbamate compounds

The main purpose of this study was to describe the kinetic properties of the cholinesterase (ChE) enzyme present in the eyes of the prawn Palaemon serratus, an abundant, ecological and commercially relevant species of European coastal environments. The obtained results suggest that the studied enzyme is a ChE and not a non-specific esterase, due to its apparent affinity for choline esters and the high sensitivity to eserine sulphate. This ChE displays a distinct preference for the substrate acetylthiocholine, showing a triphasic behaviour, with activation at low concentrations and inhibition by excess of substrate. Moreover, irreversible ChE inhibition by several organophosphate and carbamate compounds was characterized. All the irreversible inhibitions were homogeneous following a second-order rate reaction. The bimolecular rate constant ( k i ) values of ChE inhibition by the tested pesticides were also estimated and compared with available data from other invertebrate and vertebrate species. In conclusion, the results of the present study showed that prawn eyes possess only one ChE with typical properties of acetylcholinesterase, which is highly sensitive to the tested anti-cholinesterase compounds.

Field distribution and osmoregulatory capacity of shrimps in a temperate European estuary (SW Spain)

The spatial distribution of the six most common crustacean decapods in the benthos and hyperbenthos of the Guadalquivir estuary (SW Spain) has been analysed in relation to their osmoregulatory capacities (at 20 °C). Field densities along the estuarine salinity gradient revealed that, although the species studied showed salinity tolerance ranges in the field that do overlap to some extent, there was a considerable spatial and/or salinity-related segregation between several of them. Concerning their isosmotic points and their osmoregulatory salinity ranges, two main groups of species were distinguished: species with higher isosmotic points and tight ranges in osmoregulation, represented by marine species that entered the estuary from open sea and remained there for only part of the year ( Crangon crangon, Melicertus kerathurus and Palaemon serratus); and those with slightly lower isosmotic points and wide ranges in osmoregulation, represented by estuarine species which completed their life cycle in brackish water ( Palaemon longirostris, Palaemon macrodactylus and Palaemonetes varians). For all the species studied, their field distributions were clearly biased towards the lower end of the salinity ranges within which they osmoregulate. Nevertheless, individuals of the less euryhaline species ( M. kerathurus and P. serratus) were mainly found in less saline water when the estuarine gradient was displaced downstream and low salinities occurred close to the river mouth.