Common Diet Research Articles

DIFFERENCES BETWEEN POSTAL AND STAGE CAGE FOR PEKING DUCKS PERFORMANCE

This study conducted to determine the differences of Postal cage and Stage cage on the performance of Peking Duck in Body Weight, feed conversion, and mortality. The material used in the study was 600 Peking ducks were raised separately in postal cage and stage cage with common diet from DOD (Days Old Duck) to 35 day of age. This research was a quantitative descriptive research that interpreted data from research samples, analyzed by student T test that is used to compare the means of two groups that is Peking ducklings were raised in postal cage and stage cage. The results showed that the Body Weight, FCR, and Mortality of Peking Ducks were reared in Postal Cage is 1,54, 1,94 and 1,5% while the ducks that were kept in Stage Cage is 1.53, 1,95 and 2,5%. The results indicated that there was no difference in the performance (Body weight, FCR, and Mortality) of Peking ducks housed in postal and stage cage. However, based on the average values for body weight, FCR, and mortality, the postal cage provides the best results.

Dietary protein reduction with stepwise addition of crystalline amino acids and the effect of considering a minimum glycine-serine content in broiler diets

Reducing the dependency on soybean meal (SBM) is necessary to improve the sustainability of the poultry industry. Moreover, the recommendations for minimum contents of dietary Gly+Ser require further research. Two parallel studies were executed to determine the effects of replacing SBM with crystalline amino acids (CAA) to meet the amino acid requirements and to determine whether a minimum content of Gly+Ser is necessary for broiler diets. In study 1, 1,860 one-day-old male chicks were fed a common starter phase diet (22.8% CP). During the grower-1, grower-2, and finisher phases, the control CP content was reduced (up to 2.1%) with the stepwise addition of CAA (treatments 1-5). Within each feeding phase, AME, standardized ileal digestible Lys, and the minimum Met, Thr, Val, Gly+Ser, Ile, Arg, and Trp to Lys ratios were similar. In study 2, 1,488 male chickens were used in a 2×2 factorial design with the Gly+Ser content and feed ingredients used as main factors. Performance was monitored during 41 d in both studies. Reduction in CP content linearly increased (P < 0.05) BW, ADG, and ADFI in the grower-1, grower-2, and finisher phases. When the overall FCR was adjusted considering the BW differences (FCRadj); FCRadj linearly decreased with the weighted average CP (WACP) content (P < 0.001). In the lowest CP treatment, estimated dietary N utilization efficiency was improved by 10%, and the overall N excretion was reduced by 16% compared with the control (P < 0.001). The overall SBM and soybean oil intakes were linearly reduced relative to WACP (-12.0 and -20.2% in control vs. treatment 5, respectively; P < 0.001). Formulating with a minimum Gly+Ser content in the starter phase improved the FCR (P < 0.05) only in the corn-SBM-based diet. In grower-1, increasing Gly+Ser content improved the FCR independent of the feed ingredients used (P < 0.05). Crystalline amino acids can be used to partially replace intact protein reducing the dependency on SBM. Young birds may not synthesize enough Gly endogenously and a minimum content should be provided in the early phases.

Sulfur isotopic discrimination factors differ among avian tissues and diets: Insights from a case study in Gentoo Penguins

Abstract The use of stable isotopes of sulfur (δ34S) to infer avian diets, foraging habitats, and movements is relatively uncommon, resulting in a lack of information on patterns of δ34S incorporation in avian tissue. In a controlled study of Gentoo Penguins (Pygoscelis papua), we found that diet-tissue isotopic discrimination factors (Δ34Sdiet-tissue) differed among egg components and feathers synthesized from a common diet, ranging from –0.4 to –1.7‰. We also found that methodical choices such as lipid extraction and prey tissue selection influenced calculated Δ34Sdiet-tissue values. Specifically, Δ34Sdiet-tissue values were lower (i.e., more negative) when calculated using whole fish relative to fish muscle and lipid-extraction biased egg yolk, but not fish tissue, δ34S values. The Δ34Sdiet-tissue values obtained for Gentoo Penguins fed a marine fish diet were generally lower than those reported for freshwater fish consumption by Double-crested Cormorants (Phalacrocorax auritus), the only other bird species in which Δ34Sdiet-tissue has been quantified. We found support for the hypothesis that tissue Δ34Sdiet-tissue values are inversely related to dietary δ34S values in birds, similar to what has been observed in mammals. Given this relationship, the discrimination factors reported here for Gentoo Penguins may be broadly applicable to other avian species with a similar marine diet. Finally, we provide recommendations for future studies seeking to quantify Δ34Sdiet-tissue in avian tissues and guidance to allow for greater application of sulfur stable isotope analysis in ornithological research.

Interactions between animal temperament and exposure to endophytic tall fescue: Effects on cell-mediated and humoral immunity in beef heifers

Two experiments (n=12 Angus heifers/experiment) investigated influences of animal temperament, as indicated by exit velocity (EV; determined at weaning) and consumption of toxic endophyte-infected tall fescue seed on peripheral lymphocyte production of interferon-γ. Heifers were selected from calves born on the University of Kentucky’s C. Oran Little Research Center. In experiment 1, calves were randomly selected from 50 heifers within a single calf crop. In experiment 2, calves with the 6 fastest and 6 slowest EV in the subsequent year’s calf crop were selected. In both experiments, heifers were assigned to either high or low EV treatments based on relative ranking, and endophyte treatments (toxic endophyte-infected, E+, or endophyte-free, E-, fescue seed) were balanced by body weight. Rations were restricted to 1.8 x NEm and common diet was top-dressed with fescue seed each morning. Experiment 1 had four phases (pre-endophyte treatment/thermoneutral, increased room temperature, increased room temperature/endophyte treatment, and post-endophyte/thermoneutral) and experiment 2 had two phases (increased room temperature/endophyte treatment and thermoneutral). During endophyte treatment phases, heifers were fed their respective treatment seed. During all other phases, all heifers received E- seed. In experiment 1, proportions of lymphocytes producing interferon-γ were decreased in E+ heifers during the heat/endophyte phase (P=0.03) whereas during the subsequent thermoneutral period this response was greater in high, compared with low, EV heifers on E- treatment, with no difference observed among E+ heifers (interaction P=0.08). Also during the recovery phase, average lymphocyte production of interferon-γ was higher in E+ heifers (P=0.01). Consistent with experiment 1 findings, during the recovery period of experiment 2, endophyte exposure increased the per cell production of interferon-γ (P&amp;lt;0.01). In this experiment, the difference was of sufficient magnitude to result in a concomitant increase (P=0.03) in total interferon-γ production during that period. These results indicate peripheral lymphocyte production of interferon-γ can be influenced by both EV and endophyte exposure following periods of increased ambient temperature humidity indices, though there was minimal indication of interactions between temperament and alkaloid exposure. This may imply that cattle with high exit velocities and those previously exposed to toxic endophyte-infected tall fescue are better poised against cell-mediated challenges.

Effect of Zinc and Boron on Yield and Nutrient Content of Coriander

Micronutrient deficiency, especially zinc and boron deficiency, commonly occurs in Bangladesh soils. Nevertheless, common diets of this country’s people are often deficient in zinc. Thus, application of zinc and boron has significant value in enhancing crop yield as well as zinc and boron content in crop. With this understanding, field experiments were conducted in two consecutive seasons of 2018-19 and 2019-20 at Bangladesh Agricultural Research Institute (BARI) farm, Gazipur under the agroecological zone 28 (Madhupur Tract). Texturally the soil was sandy clay loam with 6.2 pH, 1.26% organic matter, 0.99 mg kg-1 zinc and 0.13 mg kg-1 boron content. Treatments consisted of a factorial arrangement of three levels of zinc (0, 2, 4 kg ha-1) and three levels of boron (0, 1, 2 kg ha-1) in a randomized complete block design (RCBD) with three replications. Combined application of Zn and B significantly increased the foliage and seed yield as well as their (zinc and boron) contents. On an average, among the treatment combinations, Zn4B1 produced the highest foliage yield (4.55 t ha-1) and Zn2B2 gave the highest seed yield (1.99t ha-1). The highest zinc and boron contents were found in Zn4B2 treatment for both foliage and seeds. Agronomic biofortification of zinc in coriander could be possible without reducing yield through the use of Zn4B1 treatment for foliage purpose and Zn2B2 for seed purpose.&#x0D; Bangladesh J. Agril. Res. 47(1): 91-98, March 2022

A Longitudinal Characterization of the Seminal Microbiota and Antibiotic Resistance in Yearling Beef Bulls Subjected to Different Rates of Gain.

In this study, we evaluated the seminal and fecal microbiota in yearling beef bulls fed a common diet to achieve moderate (1.13 kg/day) or high (1.80 kg/day) rates of weight gain. Semen samples were collected on days 0 and 112 of dietary intervention (n = 19/group) as well as postbreeding (n = 6/group) using electroejaculation, and the microbiota was assessed using 16S rRNA gene sequencing, quantitative PCR (qPCR), and culturing. The fecal microbiota was also evaluated, and its similarity with seminal microbiota was assessed. A subset of seminal bacterial isolates (n = 33) was screened for resistance against 28 antibiotics. A complex and dynamic microbiota was detected in bovine semen, and the community structure was affected by sampling time (R2 = 0.16, P < 0.001). Microbial richness increased significantly from day 0 to day 112, and diversity increased after breeding (P > 0.05). Seminal microbiota remained unaffected by the differential rates of gain, and its overall composition was distinct from fecal microbiota, with only 6% of the taxa shared between them. A total of 364 isolates from 49 different genera were recovered under aerobic and anaerobic culturing. Among these seminal isolates were pathogenic species and those resistant to several antibiotics. Overall, our results suggest that bovine semen harbors a rich and complex microbiota which changes over time and during the breeding season but appears to be resilient to differential gains achieved via a common diet. Seminal microbiota is distinct from the fecal microbiota and harbors potentially pathogenic and antibiotic-resistant bacterial species. IMPORTANCE Increasing evidence from human and other animal species supports the existence of a commensal microbiota in semen and that this seminal microbiota may influence not only sperm quality and fertility but also female reproduction. Seminal microbiota in bulls and its evolution and factors shaping this community, however, remain largely underexplored. In this study, we characterized the seminal microbiota of yearling beef bulls and its response to the bull age, different weight gains, and mating activity. We compared bacterial composition between seminal and fecal microbiota and evaluated the diversity of culturable seminal bacteria and their antimicrobial resistance. Our results obtained from sequencing, culturing, and antibiotic susceptibility testing provide novel information on the taxonomic composition, evolution, and factors shaping the seminal microbiota of yearling beef bulls. This information will serve as an important basis for further understanding of the seminal microbiome and its involvement in reproductive health and fertility in cattle.

Isorhynchophylline inhibits inflammatory responses in endothelial cells and macrophages through the NF-κB/NLRP3 signaling pathway

BackgroundAtherosclerosis is a chronic inflammatory disease of arterial wall, which is closely related to inflammatory reaction. In this study, the anti-inflammatory effect of isorhynchophylline was studied by NF- κB / NLRP3 pathway.Methods(1) ApoE−/− mice were fed with high-fat diet to establish atherosclerotic model, while C57 with the same genetic background was fed with common diet as control group. Body weight was recorded and blood lipids were detected. The expression of NLRP3, NF-κB, IL-18 and Caspase-1 in aorta was detected by Western-Blot and PCR, and plaque formation was detected by HE and oil red O staining. (2) Lipopolysaccharide interfered with Human Umbilical Vein Endothelial Cells (HUVECs) and RAW264.7 to form inflammatory model, and was treated with isorhynchophylline. The expression of NLRP3, NF-κB, IL-18 and Caspase-1 in aorta was detected by Western-Blot and PCR, and the ability of cell migration was detected by Transwell and scratch test.Results(1) the expression of NLRP3, NF- κB, IL-18 and Caspase-1 in aorta of model group was higher than that of control group, and plaque formation was obvious. (2) the expressions of NLRP3, NF- κB, IL-18 and Caspase-1 in HUVECs and RAW264.7 model groups were higher than those in control group, while isorhynchophylline decreased their expression and enhanced cell migration ability.ConclusionIsorhynchophylline can reduce the inflammatory reaction induced by lipopolysaccharide and promote the ability of cell migration.

Relative abundance and size composition of prey in the common minke whale diet in selected areas of the northeastern Atlantic during 2000-04

A total of 210 common minke whales (Balaenoptera acutorostrata) were sampled in five different areas in the northeastern Atlantic during May-June 2000-04. Analysis of forestomach contents revealed a relatively mixed diet at the population level, whereas on an individual level, each whale had fed upon mainly one species. There were significant differences in diet composition between areas and some differences between years. The importance of krill in the Barents Sea increased with latitude and krill dominated the Spitsbergen diet. Capelin dominated the diet around Bear Island and contributed considerably to the diet along the coast of northern Norway. In the latter area, herring and haddock were also a great part of the diet. The diet in the Norwegian Sea consisted of mainly mature herring, while the diet in the North Sea was dominated by sand eels and mackerel. The minke whales were found to feed on a wide range of prey sizes, apparently determined by the availability of different size classes.

Rainbow Paratha – Establishment of Common People Diet in Post Pandemic Situation as Protective Measure and Immunity Enhancer

Rainbow Paratha – Establishment of Common People Diet in Post Pandemic Situation as Protective Measure and Immunity Enhancer

Effects of Replacement Cold Press Poppy Seed (Papaver somniferum) Oil to Fish Oil at Different Proportions on the Growth Performance, Blood Parameters, and Digestive Tracks Histopathology in Juvenile Common Carp (Cyprinus carpio)

The major goal of the study is to determine the potential use of poppy seed oil as a main lipid source in common carp diets. To investigate this, five different experimental diets (PO0, PO25, PO50, PO75, and PO100) with various proportions of fish oil and poppy seed oil blended as oil sources were provided and supplied to common carp for 90 days. In triplicate groups of 60 fish, 300 common carp weighing 1.15 ± 0.06 g were randomly placed in 100 L aquariums. At the end of the feeding trial, blood and tissue samples were collected from the fish after gravimetric measurements of the fish. The growth and feed utilization performance of the fish were calculated by gravimetric measurements, and tissue samples were examined by hematological, micromorphometric, and histopathological methods. According to the results, poppy seed oil enhanced the quantity of linoleic acid (18 : 2 ω−6) in the diet, and fish fed with the diet consisting completely of poppy seed oil (PO100) had the significantly greatest growth and feed utilization performance ( P &lt; 0.05 ). Furthermore, no significant differences in hematological or liver pathology were detected between all groups that were fed with experimental diets ( P &gt; 0.05 ). However, as compared to the control group, the digestive system micromorphometry and histomorphometric measurements of the fish fed with PO100 group diets showed a significant increase ( P &lt; 0.05 ). In conclusion, it was determined that the carp fed with feeds produced by using only poppy oil as the main fat source improved their digestive system, growth, and feed utilization performance without any negative effects.

Effects of dietary sulfur amino acid levels on growth performance and intestinal immunity in broilers vaccinated and subsequently infected with coccidiosis

Coccidia vaccination is a common practice in the poultry industry. However, research is lacking regarding the optimal nutritional support for coccidia vaccinated broilers. In this study, broilers were vaccinated with coccidia oocyst at hatch and were fed with a common starter diet from 1 to 10 d. On d 11, the broilers were randomly assigned to groups in a 4×2 factorial arrangement. Briefly, the broilers were fed one of four diets containing 0.6, 0.8, 0.9, and 1.0% of standardized ileal digestible methionine plus cysteine (SID M+C), respectively, from 11 to 21 d. On d 14, the broilers from each diet group were orally gavaged with either PBS (Mock challenge) or Eimeria oocysts. Compared to PBS-gavaged broilers and regardless of dietary SID M+C levels, the Eimeria-gavaged broilers had 1) decreased gain-to-feed ratio (15-21 d, P=0.002; 11-21 d, P=0.011); 2) increased fecal oocysts (P < 0.001); 3) increased plasma anti-Eimeria IgY (P=0.033); and 4) increased intestinal luminal interleukin-10 (IL-10; duodenum, P=0.039; jejunum, P=0.018) and gamma interferon (IFN-γ; duodenum, P < 0.001; jejunum, P=0.017). Regardless of Eimeria gavage, broilers fed 0.6% SID M+C had decreased (P<0.001) body weight gain (15-21 and 11-21 d) and gain-to-feed ratio (11-14, 15-21, and 11-21 d) when compared to those fed ≥ 0.8% SID M+C. Eimeria challenge increased (P < 0.001) duodenum lesions when the broilers were fed with 0.6, 0.8, and 1.0% SID M+C, and increased (P=0.014) mid-intestine lesions when the broilers were fed with 0.6 and 1.0% SID M+C. An interaction between the two experimental factors was detected on plasma anti-Eimeria IgY titers (P=0.022), as coccidiosis challenge increased plasma anti-Eimeria IgY titers only when the broilers were fed with 0.9% SID M+C. In summary, the dietary SID M+C requirement for grower (11-21 d) broilers vaccinated with coccidiosis was ranged from 0.8 to 1.0% for optimal growth performance and intestinal immunity, regardless of coccidiosis challenge.

Inclusion of Red Macroalgae (Asparagopsis taxiformis) in Dairy Cow Diets Modulates Feed Intake, Chewing Activity and Estimated Saliva Secretion.

The current study assessed the effects of red macroalgae Asparagopsis taxiformis (AT)-included as an enteric methane inhibitor-in dairy cow diets on feed intake and eating-rumination behaviour. Fifteen early lactating Norwegian Red dairy cows were offered ad libitum access to drinking water and a total mixed ration (TMR) composed of 35% concentrate feed and 65% grass silage on a dry matter (DM) basis. The experiment lasted for 74 days with the first 22 days on a common diet used as the covariate period. At the end of the covariate period, the cows were randomly allocated into one of three dietary treatments: namely, 0% AT (control), 0.125% AT and 0.25% AT in the TMR. The TMR was offered in individual feed troughs with AT blended in a 400 g (w/w) water-molasses mixture. Eating-rumination behaviour was recorded for 11 days using RumiWatchSystem after feeding the experimental diets for 30 days. The 0.25% AT inclusion significantly reduced the DM intake (DMI). Time (min/d) spent on eating and eating in a head-down position increased with the increasing AT level in the diet, whereas rumination time was not affected. The greater time spent on eating head-down with the 0.25% AT group resulted in a significantly higher chewing index (min/kg DMI). Estimated saliva production per unit DMI (L/kg DMI, SE) increased from 10.9 (0.4) in the control to 11.3 (0.3) and 13.0 (0.3) in the 0.125% and 0.25% AT groups, respectively. This aligned with the measured ruminal fluid pH (6.09, 6.14, and 6.37 in the control, 0.125% AT and 0.25% AT groups, respectively). In conclusion, either the level of the water-molasses mixture used was not sufficient to mask the taste of AT, or the cows used it as a cue to sort out the AT. Studies with relatively larger numbers of animals and longer adaptation periods than what we used here, with varied modes of delivery of the seaweed may provide novel strategies for administering the additive in ruminant diets.

P895 Differences in ulcerative colitis patients regarding COVID-19 status

Abstract Background Severe acute respiratory syndrome coronavirus 2 is the novel coronavirus that caused the coronavirus disease 2019 (COVID-19) outbreak. Studies have increasingly reported the involvement of other organs, including the gastrointestinal system, in addition to the respiratory system. Ulcerative colitis is an inflammatory bowel disease with an unknown cause. Emerging data suggest that the gastrointestinal system may be influenced by COVID-19 via the expression of angiotensin-converting enzyme-2, but data on the association between COVID-19 and ulcerative colitis, are lacking. Methods In 6-month time (June to December 2021) 49 ulcerative colitis (UC) outpatients from Riga East Clinical University Hospital were included in a cross-sectional study. All patients were divided into groups according to medically proven COVID-19 status (COVID-19+ vs COVID-19-) in the last 6 months. Information about diet, IBD medications, food supplements, vitamins, sports activities, marital status, education level, and income level was collected. Data were analyzed with SPSS 20.0. Results Out of 49 patients, 33(63.3%) were males and 13(36.7%) were females, median age was 38.0 [IQR=17] years. Fourteen patients (28.6%: 7(50%) in each gender) were Covid-19+ within last 6 months. Median time patients suffered from COVID-19 was 7 [IQR=15.3] days. The most common symptoms were: 11(19.6%) fever, 6(10.7%) rhinitis and 5(8.9%) weakness. The most common diet among all patients was IBD diet 33(67.3%) from those 12(21.4%) were COVID-19- and 4(78.6%) were COVID-19+, p=0.00. Out of 49 patients, 25(44.6%) were using food supplements (fish oil, curcumin, collagen, zinc, calcium) of those 17(30.4%) patients were COVID-19-, 8(14.3%) COVID-19+, p=0.00. Most commonly used vitamins were vitamin D 18(64,3%), from those 15(42.9%) were COVID-19-, 3(21.4%) COVID-19+, p&amp;gt;0.5; vitamin C – 7(32,8%), from those 4(11.4%) were COVID-19-, 3(21.4%) COVID-19+, p&amp;gt;0.5. Twenty-four (49%) were doing sports, of those 18(51.4%) were COVID-19-, 6 (42.9%) COVID+; p&amp;gt;0.5. Most common sports activities were cycling 8(16.3%), running 8(16.3%) and fitness 6(12.2%). Most commonly used IBD medications were mesalazine 35(71.4%), azathioprine 7(14.3%) and biologics 4(8.16%), there were no statistically significant differences between COVID-19+ and COVID-19-. There were no statistically significant differences between marital status, education level, and income regarding COVID-19 status. Conclusion COVID-19 was less common among patients who were using food supplements and followed IBD diet.

The 'Nigerian Diet' and Its Evolution: Review of the Existing Literature and Household Survey Data.

Natural and social science studies have commonly referenced a 'typical' or 'habitual' Nigerian diet, without defining what such a diet entails. Our study, based on a systematic review of the existing literature and an analysis of household-level survey data, describes the general outline of a common Nigerian diet and how it varies based on spatial, demographic, and socio-economic characteristics. We further try to establish whether Nigeria has embarked on a dietary transition common in most modern economies, marked by a greater consumption of processed foods, fats, and sugar at the expense of traditional whole cereals and pulses. We conclude that while a traditional Nigerian diet is still relatively healthy from an international perspective, it has indeed been transitioning, with an increasing inclusion of high-energy, high-fat, and high-sugar processed foods and a related growing incidence of overweight, obesity, and diet-related non-communicable diseases.

Influence of the calcium and nutrient content of the prelay diet on egg production, egg quality, and tibiae mineralization of brown egg-laying hens from 16 to 63 wk of age

The influence of the Ca and nutrient content of the diet fed from 16 to 19 wk of age, on egg production, egg quality, and tibiae mineralization, was studied in brown egg-laying hens from 16 to 63 wk of age. The experimental design was completely randomized with 4 prelay diets organized as a 2×2 factorial with 2 levels of Ca (2.5 vs. 3.8%) and 2 standardized ileal digestible Lys (g/kg) to AMEn (Mcal/kg) ratios (DLys:ME; 2.84 vs. 3.13) as main effects. From 20 to 63 wk of age, all hens received a common diet with 2.75 Mcal AMEn/kg, 0.75% DLys, and 3.8% Ca. Each treatment was replicated 18 times and the experimental unit was a cage with 10 hens. Hen production and egg quality traits were measured by period (4 wk), feeding phase (prelay and lay), and cumulatively (16-63 wk of age) and tibiae mineralization was measured at 63 wk of age. During the prelay phase, an increase in Ca delayed egg production (P=0.065), reduced feed intake (P < 0.05), and increased BW gain (P < 0.01) and percentage of shell of the egg (P < 0.05). An increase in the DLys:ME ratio increased feed intake (P < 0.01) and reduced egg size (P < 0.01). Nutrient content of the prelay diets did not affect hen production during the lay phase, except egg weight that increased (P < 0.05) in hens previously fed the low DLys:ME ratio. Eggshell quality (weight, percentage, strength, and thickness) in this phase was better (P < 0.05) in hens previously fed 3.8% Ca. Cumulatively (16-63 wk of age), hens fed the high Ca prelay diets had better shell quality but tibiae mineralization was not affected. In conclusion, an increase in Ca content of the prelay diet from 2.5 to 3.8% improved shell quality for the entire egg cycle without showing any negative effect on hen production.

Evaluation of oxidized beta-carotene on sow and piglet immune systems, sow reproductive performance, and piglet growth.

This study aimed to determine if supplementation of oxidized-beta carotene (OxC-Beta) improved sow reproductive performance, litter growth performance, vitamin A status, and ability to alter immune cells abundance in sows and piglets, subsequent litter performance, and nursery growth performance. At approximately d 60 of gestation and through the lactation period, 194 sows (blocked by parity) were assigned to a common gestation diet or the common diet supplemented with 80ppm oxidized beta-carotene (OxC-Beta, Aviagen, Ottawa, ON, Canada). A subset of sows (n = 54 per treatment) were sampled for blood and body weight recorded at the beginning of the study, farrowing, and weaning. A blood sample was taken from a subset of piglets at birth and weaning, and all piglet weights were recorded. Blood was analyzed for vitamin A as retinol concentrations and immunoglobulin G (IgG) and immunoglobulin M (IgG) levels were assessed from the sow's blood. Twelve pigs (n = 6 per treatment) were euthanized at birth and weaning. The livers were collected and analyzed for the Kupffer cell phagocytic activity through flow cytometry. Whole blood was analyzed via flow cytometry for cluster of differentiation (CD335, CD8, and CD4). Colostrum during farrowing and milk at weaning were analyzed for IgG and IgA concentrations. Data were analyzed via SAS 9.4 using MIXED and frequency procedures where appropriate. No differences (P > 0.05) between dietary treatments were observed in sow reproductive performance, feed intake, wean to estrus interval, or piglet growth performance. No differences (P > 0.05) were observed in the plasma or liver for vitamin A. No differences (P > 0.05) were observed in the composition of the colostrum or milk. No immunological differences (P > 0.05) were observed in the piglets' liver and blood or sow antibodies in colostrum and milk. The supplementation of OxC-Beta did (P < 0.05) decrease IgM and tended (P < 0.10) to decrease IgG in sow plasma. No differences (P > 0.05) were observed in the reproductive performance of subsequent litter information from the sows. Gilt litter weaning weight and feed intake were reduced (P < 0.05) compared to sow performance. In conclusion, the supplementation of OxC-Beta at 80ppm from d 60 of gestation through lactation does not affect the reproductive performance of sows, litter growth performance, vitamin A status, piglet immune status, and antibodies or composition in colostrum and milk.

A second iron injection administered to piglets during lactation improves hemoglobin concentration, growth performance, and carcass characteristics at slaughter.

An experiment was conducted to evaluate the effects of a second injection of iron dextran administered on days 6 to 8 of age. A total of 144 crossbred pigs (equal barrows and gilts; initial age 6 to 8 d; initial body weight [BW] = 2.86 ± 0.01 kg) were assigned to either the control (CON) or an added-injection treatment (+Fe). Pigs were paired by sex and BW within a litter and randomly assigned to the iron treatment within each pair. All pigs had received an initial intramuscular (IM) injection of iron dextran (200 mg Fe) <24 h after birth. Pigs assigned to the +Fe treatment received a second IM injection of iron dextran (200 mg Fe) on days 6 to 8. All pigs were weaned at 22 to 25 d, housed 6 pigs/pen, and received a common corn-soybean meal diet. BW and feed disappearance were recorded every 2 wk. Hemoglobin (Hb) concentrations were measured at birth, initiation of experiment (days 6 to 8), weaning, and the end of the nursery and end of the study. At the end of the study, 1 pig/pen (n = 12 pigs/treatment), closest to the pen mean was selected and slaughtered for carcass characteristic measures. The individual pig served as the experimental unit for BW, Hb, average daily gain (ADG), and carcass characteristic data whereas the pen served as the experimental unit for average daily feed intake, and gain/feed ratio data. The +Fe pigs had a greater Hb at weaning (13.1 vs. 10.7 g/dL, respectively; P < 0.01) and end of the nursery (12.1 vs. 11.7 g/dL, respectively; P = 0.01) compared to CON pigs. During the finisher period, +Fe pigs had a greater ADG (0.94 vs. 0.91 kg, respectively; P = 0.05) compared to CON pigs. Overall, pigs receiving the second iron injection had an ~4% increase in ADG (P = 0.04) from weaning to the end of study. The cumulative improvement in ADG from weaning to the end of study observed for +Fe group resulted in +Fe pigs having a heavier BW at the end of the study (~3 kg; P = 0.04). Following slaughter, +Fe pigs had ~7.2% heavier trimmed loin (P = 0.04) compared to the CON pigs. In conclusion, administering a second iron injection resulted in greater Hb at weaning and the end of the nursery as well as improved growth performance from weaning to the end of study weight and increased carcass weight at slaughter.

Effects of injectable vitamin E before or after transit on receiving phase growth performance, health, and blood parameters of beef steers.

The objective was to determine the effects of injectable vitamin E (VE) before or after transit on feedlot cattle receiving performance, health, and blood parameters. Angus × Simmental steers (n = 196; body weight [BW] = 163 ± 29 kg) were utilized in a randomized complete block design. Steers were blocked by BW and randomly assigned to 1 of 3 treatments: intramuscular injections of saline pre- and post-transit (CON), intramuscular injections of VE (2,000 mg d-α-tocopherol) pre-transit and saline post-transit (PRE), or intramuscular injections of saline pre-transit and VE (2,000 mg d-α-tocopherol) post-transit (POST). Pre-transit injections were administered on day 0, and steers were transported on day 7 for approximately 4 h (348 km). After arrival, steers were fed a common corn silage-based diet in GrowSafe bunks. Final BW tended to be greater (P = 0.08) for CON steers compared with POST steers while PRE steers were intermediate. From days 7 to 63, treatment affected average daily gain (ADG) with PRE and CON steers exhibiting (P = 0.04) greater ADG compared with POST steers. Dry matter intake (DMI), water intake, and gain to feed from days 7 to 63 were not affected (P ≥ 0.17) by treatment. Day 0 serum α-tocopherol concentrations were considered marginal (2.3 ± 0.2 mg/l). A treatment × day interaction (P < 0.01) was observed for serum α-tocopherol concentrations. Serum α-tocopherol concentrations were greatest for PRE steers on day 7 (prior to and post-transit), but greater for POST steers on dys 10 and 14. Plasma ferric-reducing antioxidant potential concentrations increased (P = 0.04) for POST steers compared with CON steers and PRE steers being intermediate. Plasma non-esterified fatty acids (NEFA) concentrations exhibited a treatment × day interaction (P = 0.04) with CON and POST steers being 16% and 14% greater than PRE steers on day 14, respectively. On day 21, NEFA concentrations were greatest for POST steers compared with PRE steers and CON steers being intermediate. There was no main effect (P ≥ 0.14) of treatment on the number of bovine respiratory disease morbidity treatments. Hair cortisol concentrations were decreased (P < 0.01) 14 days after transit for PRE and POST steers compared with CON steers. Overall, injectable VE administered before or after transit increased serum tocopherol concentrations while reducing stress, but did not improve the growth performance of beef steers during the receiving phase.

Evaluation of branched-chain amino acid interactions in 10 to 20 kg nursery pigs using a central composite design.

Two groups of 240 pigs (PIC 337 × 1050, PIC Genus, Hendersonville, TN) were used to investigate the interactions between leucine, isoleucine, and valine on the growth performance of approximately 10 to 20 kg nursery pigs. At weaning, pigs were placed into 40 pens with three barrows and three gilts per pen and fed a common diet for 3 wk. On day 21 postweaning, pens were randomly assigned to 1 of 15 dietary treatments in a central composite design. Diets were formulated to various levels of standardized ileal digestible (SID) Leu, Ile, and Val by supplementing L-Leu, L-Ile, and L-Val. Levels of the branched-chain amino acids, expressed as ratios to SID Lys, ranged from 98% to 180%, 46% to 64%, and 51% to 78% for Leu, Ile, and Val, respectively. Diets were formulated to be iso-Lys, isonitrogenous, and isocaloric. Pig weights and feed intake were measured for the 21-d experiment to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency (G:F). Growth performance data were analyzed using the lm() function in R version 4.2.2 (R Core Team, 2022). The second-order polynomial model included the linear and quadratic effects of Leu, Ile, and Val, their three two-way interactions, and initial body weight. Pen was the experimental unit, and parameters were considered significant at P ≤ 0.10. A linear and quadratic effect of Val was observed for ADG and G:F (P < 0.001). There was an interaction between Leu and Ile for ADG (P = 0.069) and G:F (P = 0.032), where increasing Leu and decreasing Ile, and the inverse, improved ADG and G:F. However, growth and efficiency were negatively impacted as Leu and Ile increased in the diet. There was an interaction between Leu and Val for ADFI (P = 0.060), where Leu negatively impacted feed intake at low levels of Val but had little impact as Val increased above NRC (2012) recommendations. In conclusion, Val linearly and quadratically impacted ADG and G:F, regardless of Leu and Ile levels in the diet, while ADG and G:F were reduced with high levels of Leu and Ile, which was resolved as either Leu or Ile was reduced. Furthermore, ADFI was negatively impacted by increased Leu when Val was below NRC (2012) recommendations but was not affected by Leu at higher Val levels. Together, the results of this experiment emphasize the complexity of amino acid metabolism in nursery pigs and the importance of considering potential interactions among amino acids when conducting requirement studies.

Torula yeast may improve intestinal health and immune function of weanling pigs.

An experiment was conducted to test the hypothesis that inclusion of a conventional torula yeast or a torula yeast produced from forestry byproducts (i.e., woody torula yeast) in diets for weanling pigs instead of fish meal and plasma protein improves growth performance and intestinal health of pigs. A total of 120 weanling pigs (6.53 ± 0.78 kg) were allotted to three treatments with ten replicate pens per diet. Pigs were fed one of three diets from days 1 to 14 post-weaning (phase 1), whereas all pigs were fed a common diet in phase 2 (days 15 to 28). The three treatments in phase 1 included a control diet with 5% fish meal, 3.5% plasma protein, and no torula yeast. The second diet contained 1.5% fish meal, 14% woody torula yeast, and no plasma protein, whereas the third diet contained 1.5% fish meal, 14% conventional torula yeast, and no plasma protein. Fecal scores were assessed every other day. On day 7, one pig per pen was euthanized to collect ileal tissue and mucosa for determination of morphology and for ribonucleic acid (RNA) sequencing analysis. At the end of phases 1 and 2, blood samples were collected and concentrations of cytokines, plasma urea nitrogen (PUN), peptide YY, immunoglobulin G, total protein, and albumin were analyzed. Results indicated that both torula yeast sources could replace fish meal and plasma protein without affecting growth performance, intestinal morphology, or blood characteristics of pigs. Pigs fed a diet containing torula yeast had improved (P < 0.05) fecal scores during phase 1. Pigs fed the conventional torula yeast diet had greater (P < 0.05) concentration of interleukin-2 compared with pigs fed the control diet. On day 14, greater (P < 0.05) concentrations of interleukin-4 and interleukin-10 were observed in pigs fed the diet containing the woody torula yeast or conventional torula yeast compared with pigs fed the control diet. Results from the RNA sequencing indicated that 19 of 24 analyzed genes involved in digestion and absorption of protein and vitamins were downregulated in pigs fed the diet containing woody torula yeast compared with pigs fed the control diet. However, only two genes (i.e., ANKS4B and FAM54A) were downregulated in pigs fed the woody torula yeast diet compared with the conventional torula yeast diet. In conclusion, using woody or conventional torula yeast instead of fish meal and plasma protein in the phase 1 diet for weanling pigs may improve intestinal health without influencing growth performance of pigs.