Limanda Limanda Research Articles

The influence of continuous lines of light on the height at which fish enter demersal trawls

Fishing trials were conducted to investigate the effect of continuous lines of light on the height at which fish enter demersal trawls. A two-level trawl, with a horizontal separator panel that either directs fish to go above the panel to one codend or to go below it to another, was fished. A side-emitting fibre optic cable, illuminated by a light emitting diode (LED), was used to provide a continuous distribution of light on the leading edge of the separator panel and on the fishing line. During autumn daytime trials the illuminated cable only influenced common dab (Limanda limanda). The panel height was also varied during these trials and more lemon sole (Microstomus kitt), plaice, long rough dab (Hippoglossoides platessoides), common dab and gurnards entered the trawl above the panel when the height of its centre section was constrained to 20 cm above the fishing line. During trials held at night, in spring, the illuminated cable significantly reduced the proportion of haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), plaice (Pleuronectes platessa), common dab and gurnards (Eutrigla gurnardus and Chelidonichthys cuculus) that swim over the panel. It did not matter whether the illuminated line was attached to the leading edge of the separator panel or to the fishing line as there was no difference in how fish entered the gear between these cases.The results demonstrate how illuminated cables can be used to modify the height at which fish enter demersal trawls, direct them to different parts of the fishing gear and potentially improve the selective performance of trawl gears. They highlight that response to the panel will be species specific and may have seasonal and environmental dependencies.

Selective flatfish seine: a knee-high demersal seine barely catches cod

Abstract Norwegian coastal cod (Gadus morhua) protection restricts the use of active fishing gears. Demersal seines, acknowledged as being efficient for targeting flatfish, are therefore largely excluded from the fjords. To exploit plaice (Pleuronectes platessa), a species-selective gear that avoids catching cod is needed. We therefore designed a low-rise demersal seine with a 0.6 m vertical opening and tested it on fishing grounds in Lofoten (Northern Norway), comparing it with a conventional seine that had a vertical opening of ∼3.5 m, and fished both during the day and at night. Six to nine hauls were taken with each of the four gear/time-of-day categories (32 hauls in total). The low-rise seine caught no fewer plaice during day-time fishing, but less at night. Cod and haddock (Melanogrammus aeglefinus) catches were reduced by 94% and 98%, respectively, while catches of sole (Solea solea) increased with the low-rise seine. No catch differences were found for halibut (Hippoglossus hippoglossus), common dab (Limanda limanda), or monkfish (Lophius piscatorius). The low-rise seine therefore enables targeting flatfish while avoiding gadoid catches, although loss of plaice during night-time fishing is to be expected.

How larval traits of six flatfish species impact connectivity

AbstractConnectivity and dispersal are important factors for ecosystem dynamics, conservation, and resource management. Dispersal and recruitment success are determined in early life for many marine species. For those larvae that are pelagic, transport from spawning to nursery grounds is driven by hydrodynamic processes. Other environmental factors such as temperature and biological factors such as ecophysiology, behavior, and reproductive strategy (spawning period and spawning grounds) influence the final dispersal pattern and larval survival. We utilized a Lagrangian particle tracking model coupled with a three‐dimensional hydrodynamic model (Larvae&Co) to assess the connectivity patterns between spawning and nursery grounds of six commercially exploited flatfish species in the North Sea over a 10‐yr period (1997–2006). Standardized analyses have highlighted how spawning and nursery grounds are connected under the combined pressure of environment and life‐history traits. Results showed that the six flatfishes can be divided in two groups, each with their specific connectivity patterns. Turbot, common sole, and brill live in two subpopulations in the North Sea; common dab, European flounder, and European plaice represent a single mixed population. In general, the modeled and genetic patterns match, hence showing the strong impact of larval connectivity. The large overlap in connectivity for species that spawn during the same period and the seasonal change in hydrodynamics highlight the strong impact of a summer front in larval dispersal. Our results prove that individual‐based modeling is a powerful tool to guide resource management, even in cases of limited biological information.

Neurotoxin BMAA and its isomeric amino acids in cyanobacteria and cyanobacteria-based food supplements.

Cyanobacteria are photosynthetic microorganisms distributed globally in aquatic and terrestrial environments. They are also industrially cultivated to be used as dietary supplements, as they have a high nutritional value; however, they are also known to produce a wide range of toxic secondary metabolites, called cyanotoxins. BMAA (β-methylamino-l-alanine) and its most common structural isomers, DAB (2,4-diaminobutyric acid) and AEG (N-2-aminoethylglycine) produced by cyanobacteria, are non-proteinogenic amino acids that have been associated with neurodegenerative diseases. A possible route of exposure to those amino acids is through consumption of food supplements based on cyanobacteria. The review critically discusses existing reports regarding the occurrence of BMAA, DAB and AEG in cyanobacteria and cyanobacteria-based food supplements. It is shown that inconsistencies in reported results could be attributed to performance of different methods of extraction and analysis applied and in ambiguities regarding determination of soluble and bound fractions of the compounds. The critical aspect of this review aims to grow awareness of human intake of neurotoxic amino acids, while results presented in literature concerning dietary supplements aim to promote further research, quality control as well as development of guidelines for cyanotoxins in food products.

How benthic habitats and bottom trawling affect trait composition in the diet of seven demersal and benthivorous fish species in the North Sea

Demersal and benthivorous fish depend on prey availability in benthic habitats for their diet. Prey availability may in turn be affected by bottom trawling. Our study attempted to link the prey consumed by fish directly to those available in the benthic environment. We test the hypothesis that bottom trawling significantly affected the trait composition of fish diets with a strong link to benthic habitats. Stomach content data were analysed for seven demersal and benthivorous fish species in the southern and central North Sea and were related to habitat fauna collected by grab and epi-benthic trawl data across the same spatial extent. Biological trait analysis was used to avoid taxonomic bias in large-scale comparisons across habitats and to quantify the effect of bottom trawling, which tends to shift prey diversity in favour of small, opportunistic and short-lived species and traits. The diets of two demersal omnivores (whiting, Merlangius merlangus and Atlantic cod, Gadus morhua) and two opportunistic benthivores (haddock, Melanogrammus aeglefinus and long-rough dab, Hippoglossoides platessoides) did not appear to reflect either infaunal or epifaunal traits that were abundant in the benthic environment. The diets of European plaice (Pleuronectes platessa) and Dover sole (Solea solea), however, showed strong links with infaunal prey trait composition in shallow sand to muddy sand habitats located in the eastern North Sea and the Dogger Bank. Such strong links were also observed for the diets of Common dab (Limanda limanda) and plaice in similar habitats in deeper waters. Plaice targeted small to medium sized infaunal burrowers and deeper dwelling infaunal species. Its diet was significantly affected by bottom trawling, which caused a reduction in prey biomass in their stomachs rather than a shift in trait composition. Our study showed that the diets of benthivorous fish across large spatial scales are strongly linked with the prey trait availability in their benthic habitats and that bottom trawling significantly affected the diet of plaice, a species with strong habitat-diet associations.

First isolation of Vibrio tapetis and an atypical strain of Aeromonas salmonicida from skin ulcerations in common dab (Limanda limanda) in the North Sea.

Skin ulcerations rank amongst the most prevalent lesions affecting wild common dab (Limanda limanda) with an increase in prevalence of up to 3.5% in the Belgian part of the North Sea. A complex aetiology of these ulcerations is suspected, and many questions remain on the exact factors contributing to these lesions. To construct the aetiological spectrum of skin ulcerations in flatfish, a one-day monitoring campaign was undertaken in the North Sea. Fifteen fish presented with one or more ulcerations on the pigmented and/or non-pigmented side. Pathological features revealed various stages of ulcerations with loss of epidermal and dermal tissue, inflammatory infiltrates and degeneration of the myofibers bordering the ulceration, albeit in varying degrees. Upon bacteriological examination, pure cultures of Vibrio tapetis were retrieved in high numbers from five fish and of Aeromonas salmonicida in one fish. The V.tapetis isolates showed cross-reactivity with the sera against the representative strain of serotype O2 originating form a carpet-shell clam (Ruditapes descussatus). Moreover, the A.salmonicida isolates displayed a previously undescribed vapA gene sequence (A-layer type) with possible specificity towards common dab. Further research is necessary to pinpoint the exact role of these agents in the development of skin ulcerations in common dab.

Effect of fisher’s soak tactic on catch pattern in the Danish gillnet plaice fishery

Soak duration in the gillnet fisheries can vary from a few hours to several days. The industry reports a variation of soak tactics between target species, but also between seasons for the same species. These are determined by the robustness of the target species and the catch of unwanted species. Different soak tactics were compared to estimate the role that the choice of a soak tactic plays in the catch efficiency of both target and unwanted species. In the Danish summer gillnet fishery targeting plaice (Pleuronectes platessa), nets are deployed approximately 12h (h) during day. Unwanted species are common dab (Limanda limanda) and edible crab (Cancer pagurus). The commercially used 12h deployment during day was compared to 12h deployment during night and 24h deployment. On average, there were about 1.5 more catches of commercial size plaice (above 27cm), and 2 and 4 times less catches of the unwanted dab and edible crab, respectively, for 12h at day compared to the other soak tactics (12h at night or 24h). Gillnetters participating in the coastal summer fishery for plaice follow the theoretical optimal soak tactic. The commercially used 12h deployment during day maximises the catch of commercial sized plaice and limits handling time by catching less unwanted dab and crabs.

Detection of low numbers of microplastics in North Sea fish using strict quality assurance criteria

We investigated 400 individual fish of four North Sea species: Atlantic Herring, Sprat, Common Dab, and Whiting on ingestion of >20μm microplastic. Strict quality assurance criteria were followed in order to control contamination during the study. Two plastic particles were found in only 1 (a Sprat) out of 400 individuals (0.25%, with a 95% confidence interval of 0.09–1.1%). The particles were identified to consist of polymethylmethacrylate (PMMA) through FTIR spectroscopy. No contamination occurred during the study, showing the method applied to be suitable for microplastic ingestion studies in biota. We discuss the low particle count for North Sea fish with those in other studies and suggest a relation between reported particle count and degree of quality assurance applied. Microplastic ingestion by fish may be less common than thought initially, with low incidence shown in this study, and other studies adhering to strict quality assurance criteria.

Role of Vibrio tapetis in the development of skin ulceration in common dab (Limanda Limanda)

Role of Vibrio tapetis in the development of skin ulceration in common dab (Limanda Limanda)

Spatial and temporal analysis of the risks posed by polychlorinated biphenyl and metal contaminants in dab (Limanda limanda) collected from waters around England and Wales

The metals cadmium, mercury and lead, and the ICES7 CB levels were analysed in the common dab Limanda limanda to investigate whether concentrations of these determinants are at levels above established OSPAR assessment criteria around England and Wales. The results indicate that CB118 is above the OSPAR derived EAC at 22 out of 29 sampled stations, with 15 showing significant reductions. Cadmium is increasing significantly at six stations with two sites in the North Sea breaching corresponding OSPAR EAC. Mean results for all other PCBs and metals stayed below the EAC thresholds, with Hg concentrations even falling below BAC levels at South Eddystone located in the western English Channel. This study shows that PCBs banned over 20years ago are still found at concentrations in fish liver tissues which may pose a health risk to both the individual fish and their predators.

Mercury species in dab (Limanda limanda) from the North Sea, Baltic Sea and Icelandic waters in relation to host-specific variables

In the framework of the ICON project (Integrated Assessment of Contaminant Impacts on the North Sea), muscle tissue from a total of 135 common dab (Limanda limanda) (20–28 cm total length) was collected in seven offshore sampling areas in the North Sea, at Iceland and in the Baltic Sea during Aug/Sept and December 2008 for a chemical mercury speciation analysis by means of gas chromatography and detection by cold vapour atomic fluorescence spectroscopy (GC-CVAFS). There was a highly significant correlation between concentrations of methylmercury (MeHg+) and inorganic mercury (Hg2+) in individual fish, and the mean ratio of MeHg+ compared to Σ Hg (MeHg+ + Hg2+) was 94.0%. The results revealed statistically significant differences in concentrations of MeHg+ and Hg2+, respectively, between sampling areas. Mean concentrations in the German Bight (North Sea), in Icelandic waters and in Mecklenburg Bight (Baltic Sea) were low (MeHg+: 0.023–0.036; Hg2+: 0.001–0.002 mg/kg wet weight), while concentrations in dab from the Dogger Bank, Firth of Forth and the vicinity of the Ekofisk oil field (all North Sea) were significantly higher (MeHg+: 0.059–0.101; Hg2+: 0.003–0.004 mg/kg wet weight). Statistical correlation analysis on effects of host-specific factors revealed that neither length, weight, age, sex nor condition factor showed a significant relationship with Hg concentrations. However, Hg concentrations were significantly correlated with the Fish Disease Index (FDI), indicating a relationship between Hg concentrations and the health status of dab. Multiple linear regression analysis aiming to find factors affecting Hg concentrations revealed that only the sampling area had a highly significant main effect on Hg concentrations, and in some cases, additionally the condition factor contributed significantly to the final model. From the results, it cannot be excluded that elevated Hg concentration recorded in dab were linked to discharges from offshore oil and gas installations and that Hg affected the health status of dab.

Diseases of dab (Limanda limanda): Analysis and assessment of data on externally visible diseases, macroscopic liver neoplasms and liver histopathology in the North Sea, Baltic Sea and off Iceland

In the framework of the ICON project (Integrated Assessment of Contaminant Impacts on the North Sea), common dab (Limanda limanda) from seven offshore sampling areas in the North Sea, Icelandic waters and the western Baltic Sea were examined in 2008 for the presence of externally visible diseases and parasites (EVD), macroscopic liver neoplasms (tumours) (MLN) and histopathological liver lesions (LH). Methodologies applied followed standardised ICES and BEQUALM protocols. The EDV results revealed pronounced spatial variation, with dab from the central and northern North Sea sampling areas showing the highest disease prevalence. MLN were recorded only in North Sea dab from the German Bight, Firth of Forth and Ekofisk at a low prevalence. LH results revealed a dominant prevalence of non-specific, mostly inflammatory, lesions and a low prevalence of early toxicopathic non-neoplastic lesions, tumour pre-stages (foci of cellular alteration) and liver tumours. For the analysis and assessment of spatial variation of EVD, a Fish Disease Index (FDI) was calculated for individual dab, summarising data on the presence/absence of EDV, their severity grades, effects on the host and compensating for effects of length, sex and season. FDI data confirmed that the health status of North Sea dab from the offshore areas Dogger Bank, Ekofisk and Firth of Forth was significantly worse than in dab from the German Bight, Icelandic areas and the western Baltic Sea. An assessment of the disease data following ICES/OSPAR criteria was accomplished by applying established numeric background (BAC) and ecological assessment criteria (EAC) for EDV, MLN and LH. The combined assessment of the three disease categories indicated that health effects classified as unacceptable were rare and mainly affected dab from the North Sea.Based on the findings of the present study, it is recommended to monitor wild fish diseases in the context of assessing the impact of hazardous substances and other stressors on the marine environment. The Fish Disease Index (FDI) is regarded as a strong tool for disease data analysis and assessment, suitable as ecosystem health indicator.

Flatfishes of the White Sea: New data on the biology of the Arctic Flounder Liopsetta glacialis, European flounder Platichthys flesus, and common dab Limanda limanda

Biological characteristics have been studied in three flatfish species, the Arctic flounder Liopsetta glacialis, European flounder Platichthys flesus, and common dab Limanda limanda, collected by trawling and with fixed nets in different areas of the White Sea in 2001–2013. Flatfishes of the eastern areas of the sea are characterized by slower growth rates and a longer age series with a considerable proportion of older individuals. The greatest proportion of left-eye European flounders has been found in western areas of the sea. Pigmentation of the blind side of the body is recorded most frequently in Arctic flounders of Onega Bay.

Common dab, Limanda limanda (L.), as a natural carrier of salmonid alphavirus (SAV) from waters off north-west Ireland.

Common dab, Limanda limanda (L.), as a natural carrier of salmonid alphavirus (SAV) from waters off north-west Ireland.

Identification of a wild reservoir of salmonid alphavirus in common dab Limanda limanda, with emphasis on virus culture and sequencing

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Detection of salmonid alphavirus RNA in Celtic and Irish Sea flatfish.

Pancreas disease (PD) caused by the salmonid alphavirus (SAV) has been the most significant cause of mortalities in Irish farmed salmon Salmo salar L. over the past decade. SAV is a single-strand positive-sense RNA virus, originally thought to be unique to salmonids, but has recently been detected using real-time RT-PCR in a number of wild non-salmonid fish. In the present report, 610 wild flatfish (common dab Limanda limanda, plaice Pleuronectes platessa and megrim Lepidorhombus whiffiagonis) were caught from the Irish and Celtic Seas and screened for SAV using real-time RT-PCR and sequencing. In general, a very low prevalence was recorded in common dab and plaice, except for 1 haul in Dublin Bay where 25% of common dab were SAV-positive. SAV sequence analysis supported the fact that real-time RT-PCR detections were specific and further characterised the detected viruses within SAV Subtype I, the predominant subtype found in farmed salmon in Ireland.

Application and utility of using otolith weights in the ageing of three flatfish species

The relationship between fish length and otolith weight for the three flatfish species Plaice Pleuronectes platessa, Common sole Solea solea and Common dab Limanda limanda sampled from Poole Bay, England, were analysed to identify their utility in deriving estimates of age (in years). Following estimations of age from otolith microscopy, predictive models using discriminant function analysis and logistic regression were developed using the parameters age estimate, fish length and otolith weight. These revealed that the estimated ages from microscopy were relatively consistent with their relationship between total length and otolith weight, with agreement in the classification of age groups across the three species and their five age groups of between 69 and 100%. Agreement <100% tended to occur where there were overlaps in otolith weight and length for specific age classes. Thus, whilst use of otolith weight: fish length relationships have high utility in helping improve the accuracy of age estimates from otolith microscopy, difficulties may remain where there are overlaps in these parameters between age groups.

Hyperpigmentation in North Sea dab Limanda limanda. II. Macroscopic and microscopic characteristics and pathogen screening

An increasing trend in the prevalence of hyperpigmentation in the common dab Limanda limanda from the North Sea prompted us to investigate the potential role of infectious agents as causes or contributing factors to the condition. Dab representing 3 severity grades of hyperpigmentation were sampled for virology, bacteriology, histopathology and ultrastructure assessments. No cytopathic effect was recorded during virology testing, and bacteriological results showed no differences between normal and hyperpigmented dab. Histopathological assessment showed that the most significant changes occurred in the dermis as a result of chromatophore hyperplasia, namely melanophores and iridophores, alongside loose melanin granules. Dermal lymphocytic infiltration occasionally expanding into the epidermis and the underlying musculature was more frequent in highly pigmented dab than in normal fish, suggesting an active immune response. Ultrastructure studies showed additional disruption of the epithelial layer, with loose melanin granules between cells and a number of single or aggregated melanocytes. Dab representing different grades of hyperpigmentation kept in the laboratory alongside normal fish for a monitoring period of 18 mo showed no changes in their pigment distribution pattern, nor occurrence of new pigment in the normal fish. The current investigation found no association of hyperpigmentation in the common dab with infectious agents; therefore, understanding the cause of the condition remains a challenge which can now more reliably focus on a non-infectious origin hypothesis.

Hyperpigmentation in North Sea dab Limanda limanda. I. Spatial and temporal patterns and host effects

Hyperpigmentation is a term describing a specific pigment anomaly affecting common dab Limanda limanda in the North Sea and, less frequently, in adjacent areas, e.g. the English Channel, Irish and Celtic Seas, western Baltic Sea and Icelandic waters. Other North Sea flatfish species are also affected, but at a markedly lower prevalence. The condition is characterised by the occurrence of varying degrees of green to black patchy pigment spots in the skin of the upper (ocular) body side and pearly-white pigment spots in the skin of the lower (abocular) body side. In the course of fish disease monitoring programmes carried out by Germany and the UK (England and Scotland), a pronounced spatial pattern of hyperpigmentation has been detected in the North Sea. An increase in prevalence has been recorded in almost all North Sea areas studied in the past 2 decades. The prevalence recorded in hot spot areas of the condition increased from 5 to >40% between 1988 and 2009. Analysis of the German data indicates that the prevalence and intensity (degree of discolouration) of hyperpigmentation increase with size and age, indicating a temporal progression of the condition with size and age. Intense hyperpigmentation is associated with increased growth (length) and decreased condition factor. Potential causes of the condition (UV-B radiation nutrition, water temperature increase, demographic changes) and, in particular, of the spatial/temporal patterns recorded as well as the relationship to host-specific factors (sex, age, length, growth, condition factor) are discussed.

A method of detecting patterns in mean lengths of samples of discarded fish, applied to the self-sampling programme of the Dutch bottom-trawl fishery

Abstract Uhlmann, S. S., Bierman, S. M., and van Helmond, A. T. M. 2011. A method of detecting patterns in mean lengths of samples of discarded fish, applied to the self-sampling programme of the Dutch bottom-trawl fishery. – ICES Journal of Marine Science, 68: 1712–1718. In 2009, a self-sampling programme was organized in the Netherlands, fishers sampling ca. 80 kg of discards from randomly selected bottom trawls in the North Sea. A statistical procedure is proposed to highlight samples, trips (with multiple samples), or vessels (which may have multiple trips within a year) where extreme mean lengths of discarded fish were observed. Randomization methods were used to test for evidence of non-randomness in patterns of highlighted discard samples, e.g. repeated observations of extreme mean lengths for consecutive discard samples across trips from the same vessel. European plaice (Pleuronectes platessa), common dab (Limanda limanda), grey gurnard (Eutrigla gurnardus), and whiting (Merlangius merlangus) were considered because these were the most abundant species in most of the discard samples. A linear mixed model was used to estimate random-sample effects on the estimated mean lengths by species. These random effects were incorporated into uni- and bivariate procedures to identify extreme samples that were summed for each vessel, and the probability of observing such numbers was estimated. Excluding these samples from the dataset had marginal effects on estimated size distributions of fish.