Common Bean Genotypes Research Articles

Determining Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces from Türkiye Using SSR Markers

Assessment of genetic diversity among different varieties helps to improve desired characteristics of crops, including disease resistance, early maturity, high yield, and resistance to drought. Molecular markers are one of the most effective tools for discovering genetic diversity that can increase reproductive efficiency. Simple sequence repeats (SSRs), which are codominant markers, are preferred for the determination of genetic diversity because they are highly polymorphic, multi-allelic, highly reproducible, and have good genome coverage. This study aimed to determine the genetic diversity of 40 common bean (Phaseolus vulgaris L.) landraces collected from the Ispir district located in the Northeast Anatolia region of Türkiye and five commercial varieties using SSR markers. The Twenty-seven SSR markers produced a total of 142 polymorphic bands, ranging from 2 (GATS91 and PVTT001) to 12 (BM153) alleles per marker, with an average number of 5.26 alleles. The gene diversity per marker varied between 0.37 and 0.87 for BM053 and BM153 markers, respectively. When heterozygous individuals are calculated proportional to the population, the heterozygosity ranged from 0.00 to 1.00, with an average of 0.30. The expected heterozygosity of the SSR locus ranged from 0.37 (BM053) to 0.88 (BM153), with an average of 0.69. Nei’s gene diversity scored an average of 0.69. The polymorphic information content (PIC) values of SSR markers varied from 0.33 (BM053) to 0.86 (BM153), with an average of 0.63 per locus. The greatest genetic distance (0.83) was between lines 49, 50, 53, and cultivar Karacaşehir-90, while the shortest (0.08) was between lines 6 and 26. In cluster analysis using Nei’s genetic distance, 45 common bean genotypes were divided into three groups and very little relationship was found between the genotypes and the geographical distances. In genetic structure analysis, three subgroups were formed, including local landraces and commercial varieties. The result confirmed that the rich diversity existing in Ispir bean landraces could be used as a genetic resource in designing breeding programs and may also contribute to Türkiye bean breeding programs.

Evaluation of common bean (Phaseolus vulgaris) genotypes for resistance to common bacterial blight and angular leaf spot diseases, and agronomic performances

Common bean is the most important pulse crops worldwide and in Ethiopia for its multipurpose uses. However, common bean production and productivity is mainly constrained by common bacterial blight (CBB) and angular leaf spot (ALS) diseases. Identifying and using resistant common bean genotypes is the best option to reduce the impact of such bacterial and fungal diseases. Field experiments were carried out to evaluate the genetic resistance of 25 common bean genotypes as treatments to CBB and ALS diseases, and agronomic performances at Haramaya and Melkassa, Ethiopia, during the 2019 and 2020 main cropping seasons. The treatments were arranged in a 5 × 5 triple lattice design with three replications. Data on disease intensity, growth, grain yield and yield components were recorded. Analysis of variance indicated significant (p < 0.0001) variations existed among the genotypes for diseases intensity, grain yield and yield components. Eight genotypes, namely DAB-388, DAB-478, DRKDDRB-70, DRKDDRB-81, NUA-225, NUA-517, NUA-536 and NUA-577 attained relatively low disease severity, AUDPC and disease progress rate next to the checks Zoasho (DAB-96) and Gorossa (Biofort large seed-5) to both common bean diseases regardless of locations and seasons. The genotype DAB-525 showed a moderately resistant reaction to both CBB and ALS, and the other genotypes demonstrated variable disease resistance reactions at both locations and in the two cropping years. Similarly, genotypes DAB-388, NUA-225, NUA-517, and NUA-577 relatively executed well for grain yield and yield components at both experimental fields in the 2019 and 2020 main cropping seasons. Disease severity and grain yield variably and negatively associated with reaction groups of genotypes evaluated for reaction CBB and ALS diseases. In the study, resistance to CBB and ALS diseases and good agronomic performing common bean genotypes were identified which could be important for smallholder and commercial bean production in the study area and other similar agro-ecologies in Ethiopia. It is suggested that a large number of common bean accessions should be evaluated in CBB and ALS hot spot agro-ecologies of Ethiopia for more sources of resistance and better agronomic advantages.

Genetic variability, heritability and genetic gain of common bean (Phaseolus vulgaris L.) genotypes in response to Mexican bean weevil (Zabrotes subfasciatus Boheman) infestation

The aims of the study were to determine expected genetic gains from selection and the extent and pattern of genetic diversity of common bean genotypes to Mexican bean weevil. Three hundred bean genotypes were artificially infested with the Mexican bean weevil. The experiment was laid out in a randomized complete block design with three replications. Data on thirteen insect and seed related traits were recorded and subjected to statistical analysis. The broad-sense heritability values ranged from 68.5%–93.9% for the traits studied. The expected genetic gains from selection ranged from 5.9%-67.1% for insect related traits and from 0.2%-82.2% for seed related traits. Seed weight loss showed significant positive phenotypic and genotypic correlation with number of eggs, number of adults emerged, percent adult emergence, index of susceptibility, number of holes and first and second progeny damage. Cluster analysis classified the 300 genotypes into two major clusters and seven sub-clusters. Mahalanobis’s D2 value calculated among the sub clusters ranged from 5.6 to 191.6. There was no defined relationship between geographic origins and the pattern of genetic diversity in response to Mexican been weevil infestation. Therefore, parental selection should be made based on genetic diversity and other special merits of the genotype for the resistance attributes. The introgression of the resistance gene into the adapted improved varieties and landraces, and increasing the frequency of resistance genes through selection could be used as a strategy to improve bruchid resistance in the future

Characterization of Common Bean (Phaseolus vulgaris L.) Genotypes for Drought Tolerance

Background: Drought is a major constraint affecting the seed yield of common bean in smallholder farmers’ fields in Malawi. Therefore, there is a need to develop genotypes that can perform well under drought conditions. The study was conducted to assess the variability of common bean genotypes under stressed and optimum soil moisture conditions. Methods: Forty-three common bean genotypes were evaluated under low and optimum soil moisture in a split-plot pot experiment in 2017. Data were collected at flowering stage on five root traits and seed weight. Result: Highly significant (P less than 0.01) differences were observed among the genotypes and the genotype × water treatment interactions for the root traits and seed weight. Water stress increased hypocotyl root number, basal root number and basal root growth angle by 127.1, 11.3 and 46.1% respectively, while hypocotyl root length, basal root whorl number and seed weight were reduced by 7.1, 1.9 and 9.4% respectively. Broad sense heritability and genetic advance (%) was highest for hypocotyl root length. Genotypes CER-78, SAB-560 and SER-125 were considered tolerant to soil moisture stress and should therefore be tested in various drought conditions for release and used for genetic enhancement focusing on root traits and seed yield.

Genotypic variation in response to drought stress is associated with biochemical and transcriptional regulation of ureides metabolism in common bean (Phaseolus vulgaris L.)

Ureidic legumes such as common bean (Phaseoulus valgaris L.) plants export nitrogen from the nodules to shoots and leaves as ureides during symbiotic biological nitrogen fixation. Common bean gene encoding allantoinase (allantoin amidohydrolase, EC 3.5.2.5), is a key enzyme that catalyses the hydrolysis of allantoin to allantoic acid. It plays a role in ureide generation for export and ureide catabolism to generate a nitrogen source in sinks tissues. As such, one of the adaptive mechanisms of plants to drought stress, is associated with ureides accumulation. To identify genetic variation of common bean in response to drought stress, changes in the expression of ALLANTONAISE (PvALN) gene and ureides content were examined in the leaf tissues of the three common bean genotypes (CAL96, DAB514 and DAB541) and one tepary bean genotype (Phaseolus acutifolius A.Gray). Amongst all the genotypes, the suggested drought susceptibility in DAB514 common bean genotype, was probably attributed to a repressed PvALN expression rate which were corroborated by an impaired ureides levels, and reduced plant growth. On contrary, drought stress induced an upregulated relative expression of PvALN coupled with an increase in allantoin and allantoate in DAB541 common bean genotype. In addition, the sustained plant growth in CAL96 was probably attributed to a steady amount of allantoin synthesized under drought stress. Taken together, DAB541 and CAL96 common bean genotypes are the promising genotypes with an induced upregulated transcriptional control of catabolism and/or biosynthesis of ureides, hence potential genotypes for selection and introduction under Botswana semi-arid conditions.

Estimation of Genotype X Environmental Interaction and Grain Yield Stability in Small Red Common bean (Phaseolus vulgaris L.) Genotypes at West and Kellem Wallaga Zones of Western Oromia, Ethiopia

Estimation of Genotype X Environmental Interaction and Grain Yield Stability in Small Red Common bean (Phaseolus vulgaris L.) Genotypes at West and Kellem Wallaga Zones of Western Oromia, Ethiopia

GENETIC DIVERSITY ANALYSIS OF COMMON BEAN (PHASEOLUS VULGARIS L.) GENOTYPES USING SCOT POLYMORPHISM

Common bean (Phaseolus vulgaris L.) is legume crop of worldwide importance and due to optimal content of proteins and other essential compounds it has a potential as a functional food. Genetic diversity studies are significant from the point of obtaining information important for breeding process. The goal of present work was to analyze genetic diversity among 34 genotypes of common bean from different countries using 5 SCoT (Start Codon Targeted) markers. Altogether 82 DNA fragments were amplified, out of which 66 fragments were polymorphic with an average of 11 polymorphic fragments per primer. The highest number of polymorphic fragments was detected by marker SCoT 59 (15). The percentage of polymorphism ranged from 57.17% (SCoT 2) to 78.57% (SCoT 19) with an average of 67.3%. PIC values varied from 0.719 (SCoT 19) to 0.886 (SCoT 3) and average PIC value was 0.802. The DI values varied between 0.722 (SCoT 19) and 0.888 (SCoT3), with an average of 0.804. The dendrogram of 34 genotypes of common bean, constructed based on hierarchical cluster analysis separated genotypes into two clusters (I and II). Cluster I was formed by two subclusters. Thirty-three genotypes were included in cluster I and genotype Albena (Slovak Republic), which significantly differed from other genotypes in seed size was included in cluster II. Obtained results support the effectiveness of SCoT markers in the analysis of common bean useful for genotypes differentiating and assessment of genetic diversity in the set of common bean germplasm.

Evaluation of resistance to black bean aphid (Aphis fabae) in selected varieties and mutant genotypes of common bean (Phaseolus vulgaris)

AbstractCommon bean (Phaseolus vulgaris) is an important food crop across sub‐Saharan Africa. In Zambia, actual common bean yields are typically lower than potential yields because of the impact of invertebrate pests and plant diseases. Black bean aphids (Aphis fabae) (Hemiptera: Aphididae) negatively impact bean productivity directly by ingesting plant assimilates and indirectly by vectoring diseases such as bean common mosaic virus. Current breeding programmes aim to develop bean cultivars with improved yield and tolerance to pests. The objective of this study was to screen five common bean varieties (Rozi Koko, Mwezi Moja, Majesty, KK25 and AO‐1012‐29‐3A) and four mutation‐derived genotypes (CA 3, CA 15, CA 24 and CA 38) for resistance to black bean aphid. Commercial bean cultivars such as Kabulangeti and Carioca (variety from which all mutants were derived) were used as controls for the selected varieties and mutant genotypes, respectively. Several parameters of aphid resistance traits were assessed. Deterrence to aphid was assessed by settling preference while physical barriers to aphid feeding were evaluated by nymph survival. Reduction in palatability of phloem sap was evaluated by nymph development and mean relative growth rate. Electrical penetration graph recordings of feeding behaviour were performed in order to localise aphid‐resistant factors. Nymph development was significantly longer on AO‐1012‐29‐3A compared to Kabulangeti despite the fact that there were significantly fewer glandular trichomes on this line. The variety AO‐1012‐29‐3A can be used in genetic improvement of common bean for aphid resistance.

Number of experiments that should be considered in the cluster analysis of common bean genotypes for plant architecture and grain yield traits

The number of experiments that allows the choice of parents to be used in controlled crossings in a more assertive way in cluster analysis is unknown for plant architecture and grain yield traits in common bean. Therefore, the objective of this work was to determine the number of experiments that should be considered in Tocher's and the unweighted pair group method with arithmetic mean (UPGMA) cluster analyses to identify promising common bean parents for several plant architecture and grain yield traits. Four experiments were carried out in different years and growing seasons, in the same site. The randomized block design was used and 17 common bean genotypes with carioca (beige seed coat with brown streaks) and black grains were evaluated in relation to 12 traits related to plant architecture and five traits related to grain yield. Statistical analyses were performed with data obtained from individual and combined experiments. Significant genotype × experiment interaction was observed for most of the evaluated traits. When Tocher's and UPGMA cluster analyses was performed from data obtained in individual experiments different groups were formed. The use from data obtained in two, three or four experiments allowed greather reliability in the formation of groups. Three and two experiments are sufficient in the Tocher's and the UPGMA cluster analyses, respectively, to identify promising carioca and black common bean parents for several plant architecture and grain yield traits in a more assertive way.

Investigation of the genetic structure of some common bean (Phaseolus vulgaris L.) commercial varieties and genotypes used as a genitor with SSR and SNP markers

Common bean is a species belonging to the Phaseolus genus of the Leguminosae family. It has economic importance due to being rich in protein, vitamin A and C, and minerals. Being one of the most cultivated species of legumes, the determination of genetic diversity in bean genotypes or populations has an important role in terms of our genetic resources. The objective of this study was to evaluate the genetic structure of 94 genotypes which were cultivated in different parts of the world and our country with SSR and SNP markers. 10 SSR loci and 73 SNP primers were used for the determination of genetic structure in commercial cultivars and breeding lines. All of the SSR and SNP loci used in the study were found to be polymorphic. A total of 89 alleles were identified for 10 SSR loci. Mean number of alleles per locus (Na = 8.9), effective allele number (Ne = 3.731), Shannon information index (I = 1.468), observed heterozygosity (Ho = 0.023), and expected heterozygosity (He = 0.654) were calculated based on SSR analysis. According to the results of Bayesian-based STRUCTURE analysis using SSR and SNP data, 94 bean genotypes were genetically divided into three main clusters. According to genetic distance based UPGMA dendrogram obtained from SNP analysis, 94 bean genotypes were divided into 2 main clusters corresponding Mesoamerican and Andean gene pools. The obtained results provide important information about the genetic structures of the studied bean cultivars and breeding lines. With the obtained results, it will be possible to develop breeding programs to develop new cultivars by using our gene resources.

Genetic divergence and combined selection for traits that provide high agronomic performance in common bean lines

The selection of common bean lines with intermediate cycle, upright plant architecture and high grain yield is an important goal of breeding programs. This study proposes to examine the genetic diversity of common bean genotypes for agronomic traits, select superior genotypes for traits that provide high agronomic performance and define promising crosses. Seventeen common bean genotypes were evaluated in two growing seasons in 2019. A total of 17 agronomic traits were determined, which comprised the cycle, 12 traits related to plant architecture and four traits related to production. All traits exhibited a significant genotype × environment interaction, except for lodging and insertion of the first pod. Tocher's and the unweighted pair group method with arithmetic mean (UPGMA) cluster analyses resulted in the formation of three groups of genotypes. Tocher and UPGMA analyses do not allow identifying differences between the superior genotypes of high agronomic performance. Cultivars Fepagro Triunfo and Fepagro Garapiá and lines SM 1510 and Linhagem 110, selected by the multiplicative index, stand out for different agronomic traits. The cross between cultivars Fepagro Triunfo and Fepagro Garapiá (superior agronomic traits) is recommended for the breeding program. Recombiants with high agronomic performance could be setected from the tested hybrid combinations.

Effect of Drought and Low P on Yield and Nutritional Content in Common Bean

Common bean (Phaseolus vulgaris L.) production in the tropics typically occurs in rainfed systems on marginal lands where yields are low, primarily as a consequence of drought and low phosphorus (P) availability in soil. This study aimed to investigate the physiological and chemical responses of 12 bush bean genotypes for adaptation to individual and combined stress factors of drought and low P availability. Water stress and P deficiency, both individually and combined, decreased seed weight and aboveground biomass by ∼80%. Water deficit and P deficiency decreased photosynthesis and stomatal conductance during plant development. Maximum rates of carboxylation, electron transport, and triose phosphate utilization were superior for two common bean genotypes (SEF60 and NCB226) that are better adapted to combined stress conditions of water deficit and low P compared to the commercial check (DOR390). In response to water deficit treatment, carbon isotope fractionation in the leaf tissue decreased at all developmental stages. Within the soluble leaf fraction, combined water deficit and low P, led to significant changes in the concentration of key nutrients and amino acids, whereas no impact was detected in the seed. Our results suggest that common bean genotypes have a degree of resilience in yield development, expressed in traits such as pod harvest index, and conservation of nutritional content in the seed. Further exploration of the chemical and physiological traits identified here will enhance the resilience of common bean production systems in the tropics.

Identification of QTLs/ Candidate Genes for Seed Mineral Contents in Common Bean (Phaseolus vulgaris L.) Through Genotyping-by-Sequencing.

Throughout the ages, the common bean has been consumed by humanity as an important food staple crop and source of nutrition on a global scale. Since its domestication, a wide spectrum of phenotypic and genotypic investigations have been carried out to unravel the potential of this crop and to understand the process of nutrient accumulation along with other desirable characteristics. The common bean is one of the essential legume crops due to its high protein and micronutrient content. The balance in micronutrients is critical for the growth and development of plants as well as humans. Iron (Fe), Zinc (Zn), Copper (Cu), Manganese (Mn), Magnesium (Mg), Calcium (Ca), and Molybdenum (Mo) are some of the important micronutrients present in legumes. Thus, we aimed to investigate the quantitative trait loci’s (QTLs)/single nucleotide polymorphisms (SNPs) to identify the candidate genes associated with micronutrients through genotyping by sequencing (GBS). In our investigation, through GBS we identified SNPs linked with traits and assessed seven micronutrients in 96 selected common bean genotypes for screening nutritionally rich genotypes. Among 96399 SNPs total identified through GBS, 113 SNPs showed significant phenotypic variance, ranging from 13.50 to 21.74%. SNPs associated with most of the seed micronutrients (Mg, Mn, Fe, Ca, Cu) were found on chr3 & chr11 (Mg, Mn, Mo, Ca, Zn). The findings from this study could be used for haplotype-based selection of nutritionally rich genotypes and for marker-assisted genetic enhancement of the common bean. Further, the identified SNPs for candidate genes/transporters associated with micronutrient content may pave the way for the enrichment of seeds by employing genomics-assisted breeding programs.

Pathogenesis-related gene (PvPR1 and PvPR2) expression involved in Meloidogyne incognita parasitism and resistance reactions of common bean genotypes in host–nematode interactions

Pathogenesis-related (PR) genes are useful molecular markers, and gene expressions are activated in the reaction of plant-parasitic nematodes. Determining the PR gene expression and the resistance reaction of Phaseolus vulgaris L. genotypes against root-knot nematode are valuable for understanding plant–nematode interactions. However, resistance reactions of P. vulgaris genotypes and the gene expression of host–nematode interactions are not fully understood. Therefore, this study aimed to determine the expression of PR genes during the infection of Meloidogyne incognita on resistant and susceptible bean varieties at 1, 3, 5, and 7 days postinfection (dpi). The results revealed that one genotype gave a reaction as “immune”, and numerous genotypes were susceptible. The expression of PvPR1 and PvPR2 genes reached the highest peak at 1 dpi in nematode-infected immune plants, and the expression of PvPR1 reached 5 dpi in susceptible plants. In conclusion, PR genes involved in nematode resistance and parasitism and the immune common bean genotype have great potential in breeding programs and understanding the exact location of resistance on the gene map would be advantageous as future work.

The Impact of High Temperatures on Some Plant Growth Parameters in Some Common Bean Genotypes

The Impact of High Temperatures on Some Plant Growth Parameters in Some Common Bean Genotypes

Influence of common bean genotypes and rhizobia interaction for nodulation and nitrogen fixation

Bacteria from the rhizobia group are able to associate symbiotically with bean crop, forming nodules in the root, in which the biological nitrogen-fixing process occurs. However, the efficiency of this process has been low and it can be attributed to genetic and environmental factors. Thus, the objective of this study was to evaluate the capacity of nodulation of local varieties and commercial common bean cultivars inoculated with a Rhizobium tropici strain used in commercial inoculants and rhizobia isolates from common bean root nodules. The experiment was carried out in a factorial scheme (2x4), in a randomized block design with four replicates. It was tested two local varieties and two commercial cultivars, inoculated with the reference Rhizobium tropici strain CIAT899 and the RBZ14 strain isolated from common bean nodules grown in soils of Southern Brazil, in adapted Leonard-type pots. The CIAT899 strain promoted either higher mass of viable nodules and higher nitrogen accumulation in the aerial part. The black group local variety showed better response than the cultivar TAA Dama for nodule viability, suggesting more efficiency for nodulation. The interaction between genotypes (local varieties and commercial cultivars) and bacteria showed the specificity of the complex symbiotic relationship of biological nitrogen fixation in common bean, requiring further studies of these interactions.

DNA-barcoding and Identification for Some Common Bean and Teparry Bean Genotypes using rbcL Gene

DNA-barcoding and Identification for Some Common Bean and Teparry Bean Genotypes using rbcL Gene

SELECTION OF MESOAMERICAN COMMON BEAN LINES FOR FAST COOKING AND MINERAL BIOFORTIFICATION

ABSTRACT Developing fast-cooking, mineral-biofortified common bean lines represents an important innovation for breeding programs. Thus, this study was conducted to determine whether elite Mesoamerican common bean lines differ for cooking time and mineral concentration and to select fast-cooking, mineral- biofortified lines. The experiments were carried out in two growing seasons, in which 16 common bean genotypes were evaluated. Cooking time was determined using a Mattson cooker, and the minerals were analyzed by acid digestion. The resulting data were subjected to individual analysis of variance, combined analysis of variance, clustering by the Scott-Knott test, and selection index (multiplicative). There was a significant genotype × environment interaction effect for cooking time and the concentrations of potassium, iron, zinc and copper. Only the phosphorus concentration showed no genetic variability between the common bean lines. The ranking of the best common bean lines differed for all traits examined in the different environments, according to the Scott-Knott test. The use of the multiplicative selection index allows selecting superior common bean lines for all traits, namely, DF 07-11, DF 08-21, DF 08-20 and DF 08-28. These lines exhibit a very short cooking time (up to 15 min), high potassium concentration (&gt; 12.00 g kg-1 of dry matter) and the highest mean values of micromineral concentration, and will thus be selected by the breeding program.

Host Resistance to Uromyces appendiculatus in Common Bean Genotypes.

Rust, induced by the fungus Uromyces appendiculatus, is one of the most serious bean diseases. The involved mechanisms in rust resistance were evaluated in 10 common bean genotypes during the 2019/2020 and 2020/2021 growing seasons. The disease parameters such as final rust severity (FRS%), area under the disease progress curve (AUDPC) and disease increase rate (r-value) were lower in the resistant genotypes than in highly susceptible genotypes. Biochemical compounds such as total phenols and the activity of antioxidant enzymes such as catalase, peroxidase and polyphenol oxidase were increased in the resistant genotypes compared to susceptible genotypes. In the resistance genotypes, the levels of oxidative stress markers such as hydrogen peroxide (H2O2) and superoxide (O2•−) increased dramatically after infection. The electrolyte leakage percentage (EL%), was found to be much greater in susceptible genotypes than resistant genotypes. The resistant gene SA14, which was found in genotypes Nebraska and Calypso at 800 bp, had an adequate level of resistance to bean rust with high grain yield potential. After infection, the transcriptions levels of 1,3-D-glucanases and phenylalanine ammonia lyase) were higher in the resistant genotypes than susceptible genotypes. In conclusion, the resistant genotypes successfully displayed desirable agronomic traits and promising expectations in breeding programs for improving management strategies of common bean rust disease. The resistance was mediated by antioxidant enzymes, phenolic compounds, and defense gene expressions, as well as the resistant gene SA14.

Temporal progress of web blight in three common bean genotypes on the central coast of Ecuador

Web blight caused by Rhizoctonia solani Kuhn is one of the main common beans (Phaseolus vulgaris L.) diseases of the Ecuadorian central coast. Thus, the present study aimed to evaluate the progress of web blight incidence using nonlinear models in promising genotypes SER 03 and SER 08, using the commercial cultivar INIAP 473 as a control. This study was carried out between the 2010 and 2012 seasons in Quevedo, Ecuador. Epidemiological parameters such as initial disease (y0), disease progress rate (r), final incidence and the area under the disease progress curve were estimated using the Exponential, Logistic, and Gompertz models. In general, progress curves, epidemiological parameters and other evaluated plant health variables were lower in the INIAP 473 cultivar than the other genotypes in 2011 and 2012, especially in the last season when higher y0 and r were observed in the commercial cultivar. It seems that soil and seeds inoculum and temperature play an important role in standard bean crop disease progress. While the response of genotypes and each location conditions influenced the epidemic modeling, the Logistic model better described the web blight progress under these conditions.