Decorative fountains are a common element of urban architecture and are frequently used by inhabitants as recreational water reservoirs. The presence of potentially pathogenic microorganisms in the water, as well as lack of sanitary and epidemiological supervision over such objects, poses a risk of acquiring a water‐borne infection. Hence, the aim of the study is to assess the microbiological and mycological contamination of fountain water. Water samples are collected from 10 fountains located in the city of Lodz (Poland), in 2016 twice: June/July (first sampling period) and September (second sampling period). Bacteriological analysis of water samples is carried out in accordance with standard procedures of the Commission Directive (EU) 2015/1787. Additionally, the material is examined for the presence of potentially pathogenic microscopic fungi by standard mycological protocols. In the first sampling period, 15.6% Escherichia coli and 74.3% of other coliform bacteria are detected in total isolates, while coliform bacteria accounts for 43.8% and E. coli 5.6% of the total in samples taken in September. In addition, 82 strains of fungi are isolated from the water samples, which are classified into yeasts (5 species) and filamentous fungi (17 species). Of the isolated fungi, 36.4% are potential pathogens from biosafety level 2 (BSL‐2). Aspergillus fumigatus is dominant among filamentous fungi and Candida albicans among yeasts. Contaminated fountains are hidden sources of infection for humans and represent an epidemiological problem of public health. The presence of fecal bacteria and fungal species classified into BSL‐2 in the fountain water indicates the need to introduce seasonal microbiological monitoring and implement legal regulations regarding its sanitary condition.