Commercial Waters Research Articles

Fluorescence Analysis of Quinine in Commercial Tonic Waters.

Quinine is known for treating malaria, muscle cramps, and, more recently, has been used as an additive in tonic water due to its bitter taste. However, it was shown that excessive consumption of quinine can have severe side effects on health. In this work, we utilized fluorescence spectroscopy to measure the concentration of quinine in commercial tonic water samples. An external standard method was used to calculate the concentrations of quinine in two commercially available tonic water brands, namely Canada Dry and Schweppes, and compare them to the maximum allowable concentration of quinine in beverages. Upon analysis of the data collected by five different groups, the levels of quinine were found to be above the average concentration in most commercial tonic water samples, but below the maximum permitted concentration. Moreover, the five replicate sets of data demonstrated high reproducibility of the method employed in this study. The simple yet instructive protocol that we developed can be adapted to determine the concentration of other fluorescent compounds in foods and beverages. Further, the presented method and detailed protocol can be easily adopted for undergraduate labs and in chemical education.

Preventive potential of arginine incorporated in fancy waters for erosive tooth wear

Objective(s)The aim of the study was to examine the potential of arginine (Arg)-enriched fancy waters in preventing erosive tooth wear. MethodsL-arginine (2 % w/v.) was added to five commercial fancy waters: Oasis-Lemon, Oasis-Lemon Mint, Perrier-Lemon, Perrier-Grapefruit, Pellegrino-Lemon; and deionized water served as a negative control. The pH, buffer capacity of added Arg, and F− concentrations were measured. Tooth specimens were prepared and baseline volumetric assessment (T0) was conducted using micro-CT. Subsequently, the specimens were immersed in fancy waters for 72 h, kept in an incubator (37 ℃) and solutions were changed every 24 h. After the experimental cycle (T1), 3D volumetric analysis was performed, and 3D structural images were reconstructed for qualitative assessment. ResultsThe pH and F− concentrations of fancy waters with Arg were significantly higher than the control (p < 0.001). The buffer capacity of added Arg with Perrier-Grapefruit was significantly higher than Oasis-Lemon, Oasis-Lemon Mint & Pellegrino-Lemon (p < 0.05), except for Perrier-Lemon (p > 0.05). At T1, the specimen volume in Oasis-Lemon Mint (+/− Arg) and Pellegrino-Lemon (control) was significantly lower than T0 (p < 0.05). At T1, the specimen volume of Oasis-Lemon Mint and Pellegrino-Lemon (control) was significantly lower than Arg-containing fancy waters (p < 0.05). Surface contrast indicating wear was evident from T0 to T1 in specimens from the Oasis-Lemon Mint (+/− Arg) and Pellegrino-Lemon (− Arg). ConclusionIncorporating Arg in fancy waters reduces the potential of erosive tooth wear associated with these beverages. Clinical significanceConsuming fancy water beverages (flavoured sparkling/carbonated) can lead to erosive tooth wear in young people. Incorporating Arg in fancy water beverages can prevent the erosive wear potential of these beverages while alleviating the burden of oral care on individuals at risk to erosive wear.

Preparation and characterization of stationary phase gradients on C8 liquid chromatography columns

Continuous C8 stationary phase gradients are created on commercial Waters Symmetry Shield RP8 columns by strategically cleaving the C8 moieties in a time-dependent fashion. The method relies on the controlled infusion of a trifluoroacetic acid/water/acetonitrile solution through the column to cleave the organic functionality (e.g., C8) from the siloxane framework. The bond cleavage solution is reactive enough to cleave the functional groups, even with polar groups embedded within the stationary phase to protect the silica. Both the longitudinal and radial heterogeneity were evaluated by extruding the silica powder into polyethylene tubing and evaluating the percent carbon content in the different sections using thermogravimetric analysis (TGA). TGA analysis shows the presence of a stationary phase gradient in the longitudinal direction but not in the radial direction. Two different gradient profiles were formed with good reproducibility by modifying the infusion method: one exhibited an ‘S’-shaped gradient while the other exhibited a steep exponential-like gradient. The gradients were characterized chromatographically using test mixtures, and the results showed varied retention characteristics and an enhanced ability to resolve nicotine analytes.

Electrochemical Detection of Bisphenol A Based on Gold Nanoparticles/Multi-Walled Carbon Nanotubes: Applications on Glassy Carbon and Screen Printed Electrodes.

Bisphenol A (BPA) has been classified as an endocrine-disrupting substance that may cause adverse effects on human health and the environment. The development of simple and sensitive electrochemical biosensors is crucial for the rapid and effective quantitative determination of BPA. This work presents a study on electrochemical sensors utilizing gold nanoparticle-modified multi-walled carbon nanotubes (CNT/AuNPs). Glassy carbon electrodes (GCEs) and screen-printed electrodes (SPEs) were conveniently modified and used for BPA detection. AuNPs were electrodeposited onto the CNT-modified electrodes using the galvanostatic method. The electrodes were properly modified and characterized by using Raman spectroscopy, cyclic voltammetry (CV), and electrochemical impedance analysis (EIS). The electrochemical response of the sensors was studied using differential pulse voltammetry (DPV) and constant potential amperometry (CPA) for modified GCE and SPE electrodes, respectively, and the main analytical parameters were studied and optimized. Problems encountered with the use of GCEs, such as sensor degradation and high limit of detection (LOD), were overcome by using modified SPEs and a flow injection device for the measurements. Under this approach, an LOD as low as 5 nM (S/N = 3) was achieved and presented a linear range up to 20 μM. Finally, our investigation addressed interference, reproducibility, and reusability aspects, successfully identifying BPA in both spiked and authentic samples, including commercial and tap waters. These findings underscore the practical applicability of our method for accurate BPA detection in real-world scenarios. Notably, the integration of SPEs and a flow injection device facilitated simplified automation, offering an exceptionally efficient and reliable solution for precise BPA detection in water analysis laboratories.

Potential Benefits of a Cosmetic Ingredient Combining Thermal Spring Water and Diatom Algae Extract

The development of cosmetic ingredients improving the management of skin with irritation proclivity is an actual need. However, medical recommendations in specific cases, such as sensitive skin, include the use of as few and low-reactive ingredients as possible. In this context, we here describe the development of a new ingredient consisting of a mixture of thermal water and a diatom algae extract. First, we characterized a thermal spring water (La Solia, LS-TSW). Attending to its chemical composition, LS-TSW displayed an exclusive combination of different inorganic elements, with interesting potential properties when compared with other commercial spring waters. Then, LS-TSW hydrobiome was studied, and after finding specimens of Phaeodactylum-like sp., we proposed potentiating LS-TSW benefits with its combination with an oil extract of Phaeodactylum tricornutum (PtOE). Finally, we assessed the potential of the mixture during pro-inflammatory stimulation, in the first instance using an immune cell model, and then in an in vitro system mimicking keratinocytes under skin irritation. In the last-mentioned model, the ingredient of interest effectively attenuated the induced levels of different pro-inflammatory mediators (IL-6, IL-1, TNFα, NF-κB, and CCL1), at the level of gene expression. Thus, our results highlight the potential benefits of this combination in the context of skin irritation, opening roads for its use in new skincare regimens, and addressing an important dermatological concern.

Surface multi-walled carbon nanotube modified quaternary amine-functionalized polymers for purification and determination of glyphosate and its four metabolites in plasma samples

The present study focused on the pretreatment and detection of GLY and its four metabolites AMPA, N-acetyl AMPA, N-methyl GLY and N-acetyl GLY in plasma samples. Multi-walled carbon nanotube-modified quaternary amine-functionalized polymers (QA-PDNV@MWCNTs) were synthesized in a controlled manner by self-assembly, and its morphology and composition were extensively characterized. The QA-PDNV@MWCNTs microspheres were then used as an SPE adsorbent for the preparation and rapid determination of GLY and its four metabolites in plasma samples combined with ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). The SPE conditions based on QA-PDNV@MWCNTs were optimized for GLY and its metabolites to obtain the best purification efficiency. The experimental results show that when the adsorbent contains 8% MWCNTs, it can balance the adsorption of target analytes and the purification performance of the adsorbent for impurities. In addition, this study compared the QA-PDNV@MWCNTs based SPE method with the commercial Waters Oasis MAX SPE cartridge and the results showed that the developed method in this study has better resistance to matrix interference. Under optimal conditions, the recoveries of GLY and its metabolites spiked in plasma were 82.6-99.4% with relative standard deviations (RSDs) of 1.0-7.8%. The limits of detection (LODs, S/N≥3) and limits of quantification (LOQs, S/N≥9) of the method were 0.05-0.33 μg/L and 0.15-1.00 μg/L, respectively. Finally, the developed QA-PDNV@MWCNTs based SPE-UPLC-HRMS method was used to confirm GLY poisoning not only on the basis of the detection of the GLY prototype, but also on the basis of its four metabolites.

Hydro-geochemical characterization of the main European mineral water brands

The history of the mineral water consumption goes back to early legends. Already then, in addition to physiological needs, their beneficial effects were also used in medicine. Mineral water contains a complex of components, the composition of each mineral water varies according to its origin, the minerals, salts, gases and radioactive elements it contains and the quantities of the components. In our research, statistical methods were used to analyze the chemical composition of the macro-parameters indicated on the labels of European bottled and commercial mineral waters. A total of 692 mineral waters from 13 countries were analyzed: Austria (15), Belgium (8), Greece (10), France (33), Poland (34), Portugal (7), Spain (66), Slovakia (11), United Kingdom (9), Hungary (33), Germany (309), Italy (123), Romania (34), United Kingdom (9). Furthermore, mineral waters were classified according to their evaporation residue and ionic content. Correlation coefficients were used to study the relationship between the chemical composition of rocks and mineral waters. Statistical analysis was carried out on the cations Ca2+, Mg2+, Na+, K+, and on the anions HCO3-, Cl-, SO42-. Piper diagrams of the studied mineral waters were prepared at country level, as well as box-plots and Spearman correlations and heat maps.

Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry

In cases of low concentration paraquat (PQ) poisoning, as the disease progresses over a long period of time, the prototype PQ may not be detected in the urine, which has a significant negative impact on the precise treatment of the poisoning. But PQ poisoning can also be confirmed by the detection of specific metabolites of PQ in the urine samples. In the present study, core-shell amphiphilic carboxyl-functionalized magnetic polymer microsphere (Amphiphilic-MPs-COOH) was prepared, and the as-prepared Amphiphilic-MPs-COOH was characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermogravimetry and differential thermogravimetry analyses (TG-DTG). Then, the Amphiphilic-MPs-COOH was employed as a magnetic solid-phase extraction (MSPE) adsorbent for pretreatment and rapid determination of PQ and its four metabolites from urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). The extraction-elution conditions of Amphiphilic-MPs-COOH towards PQ and its metabolites were optimized in spiking urine samples to obtain the best MSPE efficiency. The adsorption mechanism of PQ and its metabolites by Amphiphilic-MPs-COOH involves electrostatic attraction and π-π stacking interactions. Moreover, the effect of different ratios of hydrophilic monomer NVP and functional monomer 4-VBA on the extraction and purification performance of PQ and its metabolites in urine samples. And the results revealed that both hydrophilic monomer and functional monomer were important for the adsorption of PQ and its metabolites, and the addition of the appropriate amount of the hydrophilic monomer NVP can improve the compatibility of the adsorbent with the urine substrate. In addition, this study compared the matrix effect of the Amphiphilic-MPs-COOH based MSPE method and the commercial Waters Oasis WCX SPE method. The results showed that the Amphiphilic-MPs-COOH based MSPE method developed in this paper had better resistance to matrix interference. Under optimal conditions, the recoveries of PQ and its metabolites were ranging from 84.5 to 103%, with relative standard deviations (RSDs) of 1.1–6.3%. While the limits of detection (LODs, S/N ≥ 3) and limits of quantification (LOQs, S/N ≥ 9) of the method were in range of 0.1–1.6 μg/L and 0.3–4.8 μg/L, respectively. Finally, the established MSPE-UPLC-HRMS method in this study was used to confirm PQ poisoning not only based on detecting PQ prototype, but also on its four metabolites, providing strong technical support for clinical precision treatment.

Investigation on chlorogenic acid stability in aqueous solution after microwave treatment

Recently, several health benefits associated with the consumption of foods rich in chlorogenic acid (5-CQA) have been reported. However, an important issue is its low stability during extraction and food processing, resulting in isomerization to neochlorogenic and cryptochlorogenic acids and the formation of further degradation products. This work describes the evaluation of 5-CQA reactivity in commercial waters after microwave treatment. An optimized HPLC-UV method was used to monitor 5-CQA conversion to its main isomers, while LC-HRMS/MS was performed for the elucidation of transformation products. Results revealed different degrees of isomerization in 5-CQA depending on the water sample, and the formation of oxidation derivatives of CQA isomers. This study highlights the importance of analytical monitoring of food compounds, during microwave treatment for example. In the case of 5-CQA, understanding of the degradation process would allow better preservation of bioactive constituent in foods and beverages and health promoting effects.

Qualities of groundwater source used for production of commercial bottled waters in Korea

Qualities of groundwater source used for production of commercial bottled waters in Korea

Spatial distributions of natural radionuclide concentrations of bottled mineral water: doses estimation and health risk assessment

Background: Evaluation of the various types of water reveal that groundwater comprises 99% of the earth’s available fresh water. Many factors affect the type and degree of mineralization, as well as the natural radionuclides content in these types of water. The consumption of bottled natural mineral water, which comes from groundwater, among Iranians is gradually increasing. Therefore, the detection of high concentrations of radionuclides, associated with consumption of groundwater, is proposed as a public health problem in several areas. Methods: In this study, the activity concentration of natural radionuclides such as 226Ra, 228Ra, 210Pb, and 40K, annual effective dose for three age groups (&lt;1 year, 7-12 years, and &gt;17 years), and excess lifetime cancer risk due to the ingestion of natural radionuclides present in 70 different commercial bottled mineral waters from most provinces of Iran, were evaluated. Activity concentrations were measured using gamma spectrometry and a high purity germanium detector (HPGe). Results: The results showed that the activity concentrations of natural radionuclides were higher than those reported in the same studies in other countries. Also, the annul effective dose for the three age groups was much higher than the recommended value (0.1 mSvyr-1), as reported by the WHO. The excess lifetime cancer risk for three radionuclides, 226Ra, 228Ra, and 40K, were less than the acceptable value of 10-3 for radiological risk, while the risk for 210Pb was higher than the recommended value. Conclusion: According to the results of this study, the frequent use of bottled waters produced in different provinces of Iran possess health hazards to consumers.

Prevalence of per- and polyfluoroalkyl substances (PFASs) in drinking and source water from two Asian countries

The present study focuses on the determination of the occurrence and levels of per- and polyfluoroalkyl substances (PFASs) in the drinking and source water from the Philippines and Thailand. A total of 46 samples (18 commercial bottled waters, 5 drinking water from vending machine (re-fill stations) and 23 source water) were analyzed using liquid chromatography with tandem high-resolution mass spectrometry. Using the targeted method, 12 different PFASs were detected in the drinking water samples with total quantifiable PFASs (∑PFASs) levels ranging from 7.16 to 59.49 ng/L; 15 PFASs were detected in source water with ∑PFASs ranging from 15.55 to 65.65 ng/L. A 100% detection frequency was observed for perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorosulfonic acid (PFOS) in all water samples. Six other PFASs, not included in the targeted analysis, were detected using the suspect screening approach. For the first time, the presence of 2-(N-methylperfluorooctanesulfonamido) acetic acid (N-MeFOSAA) in drinking water is reported, and 3 novel PFASs (C5H5OF8, C6H4O2F6, and C9H2O2F16) were detected using suspect screening in source water. Combined results from target and suspect screening analysis showed that PFASs detected were predominantly (52%) short-chain (with fluorinated alkyl chain of ≤6) which could be explained by their high mobility in the environment. The detected PFASs levels in drinking water will not likely pose immediate health risk to consumers according to US EPA health advisory for PFOS and PFOA of 70 ng/L, but inclusion of bottled and drinking water from re-fill stations in monitoring programs is warranted.

The ecotoxicological aspects of using the marine benthic invertebrates skeleton in the production of active calcium substances

Marine benthic invertebrates are susceptible to the accumulation of heavy metals and other pollutants from the environment, leading to the impossibility of their use as raw materials in the food production and pharmaceutical industry. In this paper, the accumulation of heavy metals in the shells and needles of Strongylocentrotus sea urchins was studied to assess the safety of the use of this raw material in the production of active calcium substances for food and medical purposes with the use of voltammetry analysis and capillary electrophoresis. High levels of strontium accumulation in the studied samples were revealed, indicating the unfavorable situation of commercial waters with respect to the presence of mobile forms of strontium.

The Use of Different Commercial Mineral Water Brands to Produce Oil-In-Water Nanoemulsions.

Nanoemulsions are submicron-size colloidal systems that have the ability to encapsulate, protect, and deliver active ingredients. They have been used in the pharmaceutical, cosmetics, and food industries to improve the absorption of drugs by the skin or via the gastrointestinal tract, aide in food conservation, and treat skin problems. To proper formulate a nanoemulsion, it is important to know the characteristics of its components (aqueous and oil phases, surfactants and additives), as well as the influence on the production method that will be used. This study investigates the influence of aqueous phase composition, stability and particle size in an oil-and-water nanoemulsion formation. By using a low energy method, the purified water was exchanged for different commercial mineral water and saline solutions, and the results of stability, particle size, pH and conductivity tests, were compared. These results show that the minerals present in commercial waters may alter the particle size, pH and conductivity values of nanoemulsions, as well as their stability.

Composite films with UV barrier capacity to minimize flavored waters degradation

Abstract Secondary packages for commercial flavored waters were developed from composite films to reduce beverage UV degradation. Bottle packs of orange and grapefruit flavored waters (OFW and GFW), were wrapped with films based on high density polyethylene, poly(styrene- b -butadiene- b -styrene), and thermoplastic corn starch (0 and 5% w/w bentonite). A commercial protective film (CPF) was employed as reference. Packs were placed in a UV chamber, and the effect of radiation on beverages was evaluated by colorimetry, UV–vis spectroscopy, vitamin C content (VCC), and total phenolic compounds (TPC). Wrapping films minimized OFW color variation, being this effect more evident employing composite films and comparable to CPF. Use of composite materials as secondary packages reduced the oxidative degradation of natural juices compounds, which is the main responsible of the changes observed by UV–vis spectroscopy. VCC in both flavored waters was too low, therefore after 6 days UV irradiation it could not be detected. Regarding TPC, no protective effect of all studied films was evidenced. Composite films containing 5% w/w bentonite increased UV barrier capacity of studied matrices, being the protective effect more efficient than the corresponding to CPF.

Carbon Dioxide in Bottled Carbonated Waters and Subsequent Bubble Nucleation under Standard Tasting Condition.

Experimental and theoretical developments, including gas-liquid thermodynamics and bubble nucleation, were made relevant to the conditioning and service of three various commercial carbonated bottled waters holding different levels of dissolved carbon dioxide comprised between about 3 g L-1 and 7 g L-1. The strong dependence in temperature of the partial pressure of gas-phase CO2 found within the three batches of bottled carbonated waters was determined. Moreover, in a glass of carbonated water, the process by which the diffusion of dissolved CO2 in tiny immersed gas pockets enabled heterogeneous bubble nucleation was formalized, including every pertinent parameter at play. From this assessment, the minimum level of dissolved CO2 below which bubble nucleation becomes thermodynamically impossible was determined and found to strongly decrease by increasing the water temperature and size of the gas pockets acting as bubble nucleation sites. Accordingly, the total number of bubbles likely to form in a single glass of sparkling water was theoretically derived to decipher the role played by various key parameters. Most interestingly, for a given level of dissolved CO2, the theoretical number of bubbles likely to form in a glass was found to increase by increasing the water temperature.

Educação Ambiental: composição química de águas minerais relacionados aos elementos da tabela periódica

A educação ambiental está entre os temas transversais que devem ser contemplados durante a escolarização. Uma maneira de abordar a educação ambiental é a interdisciplinaridade, relacionando os diferentes temas propostos pelos Parâmetros Curriculares Nacionais aos conteúdos formais de cada disciplina. No presente trabalho, foram realizadas atividades pedagógicas em aulas de química, com objetivo de relacionar a composição química de águas minerais comerciais, de diferentes marcas, aos elementos da tabela periódica, de modo a promover o ensino de química integrado à educação ambiental. O público alvo foram alunos do nono ano do ensino fundamental, com idades entre 13 e 15 anos de uma escola da rede particular do Rio de Janeiro. A metodologia consistiu na apresentação das características da água potável, seguida da análise de rótulos de águas minerais comerciais, sendo que os alunos deveriam identificar os elementos e substâncias presentes, com as respectivas concentrações. Os argumentos que embasaram a análise discente dialogaram com referenciais teóricos epistemológicos previamente selecionados, com base na transversalidade e interdisciplinaridade. Os alunos relataram que nunca tinham observado anteriormente o rótulo de uma garrafa de água mineral, e, após a prática, perceberam a quantidade de informações presentes no mesmo, que remetiam ao assunto estudado na aula sobre a tabela periódica. O professor norteou uma discussão na qual foi feita uma correlação entre o local da fonte de água e sua composição química, dando ênfase à importância da preservação das fontes naturais e da variedade de diferentes águas minerais existentes, relacionadas inerentes ao local no qual elas se encontram.

Uranium and radium isotopes in some selected thermal, surface and bottled waters in Vietnam

Uranium and radium isotopes were measured in 17 water samples (6 thermal, 3 stream and 8 commercial bottled waters) using LSC and alpha spectrometer after chemical separation. The thermal and stream water samples were collected from Kim Boi spa Hoa Binh, North Vietnam. The measured radioactivity ranged ≤ 0.5 ÷ 9.0 mBq/L for 238U, 1.60 ÷ 18.6 mBq/L for 234U, ≤ 5 ÷ 29.2 mBq/L for 226Ra. For the bottled water 234U was ≤ 3 mBq/L and 238U undetected, the 226Ra ranged 5 ÷ 202 mBq/L and 228Ra ≤ 20 ÷ 115 mBq/L. For studied thermal water the increasing tendency of Ra concentration with depth water formation is observed.

Simultaneous Determination of Inorganic Anions and Cations in Water and Biological Samples by Capillary Electrophoresis with a Capacitive Coupled Contactless Conductivity Detector Using Capillary Filling Method.

An analytical method for concurrent analysis of inorganic anions and cations has been developed using a capillary electrophoresis (CE)-capacitively coupled contactless conductivity detector (C4D) system. Although hydrodynamic and electrokinetic injection techniques have been widely used in CE, we employed a capillary filling method (CFM) for the analysis of inorganic ions. The procedure is relatively simple and has the advantage that CMF does not require pressure control and vial exchange. Three anions (chloride, sulfate, nitrate) and five cations (ammonium, potassium, sodium, magnesium, calcium) were successfully separated and detected at ppm levels within 80 s using a 9 mM histidine/15 mM malic acid (pH 3.6) containing 50 mM N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate as background electrolyte. Applying this analytical condition, the electroosmotic flow is negligible and anions and cations were migrated concurrently to different polarities according to their electrophoretic mobility. Obtained raw data showed stepwise increases in detected conductivity due to the migration of sample components, which expresses as peak profiles by differentiation of electropherograms. The RSD values of the peak area and migration times for the anions and cations were satisfactory and were less than 5.15 and 2.04%, respectively. The developed method was applied for the analysis of inorganic anions and cations in commercial mineral waters, tap water, urine, and exhaled breath condensate. These results indicate that the CE-C4D system with CFM is suitable for the rapid analysis of inorganic anions and cations in various samples.

Sugar-specific carbon isotope ratio analysis of coconut waters for authentication purposes.

The application of sugar-specific carbon isotope analysis by combining high performance liquid chromatography and isotope ratio mass spectrometry is described, for investigating the detection of added C4-plant sugars in coconut waters. Authenticity of coconut waters gains more importance since the product is considered a juice by the European Fruit Juice Association (AIJN), while it holds an increasing consumer preference as healthy, low-carb beverage. The detection potential was compared with the conventional total sugar carbon isotope analysis and it is demonstrated that the isotopic profile of individual sugars substantially improves the limit of detection of added C4-plant sugars in coconut water. The study includes 30 authentic coconut waters (extracted from coconuts in the lab), which provide the authentic carbon isotope range of pulp, total sugars, sucrose, glucose and fructose, and 24 commercial coconut waters (bottled) purchased from grocery stores. The market scan revealed that 38% of the tested samples contain undeclared added C4-sugars.