Commercial Products Research Articles

Influence of gut microbiome composition on effect variations of anti-cholesterol treatment among individual mice

This study investigated if the variation in the effect of anti-cholesterol (AC) treatment on individual mice are related to gut microbiome composition. The bile salt hydrolase (BSH) activity of 23 commercial fermented milk products was examined to select a fermented milk product for AC treatment. Mice were fed to different diets for 6 weeks: high-fat (660 % of total calories from fat; D1), high-dietary fibre (20 % cellulose; D2), and low-fat (17.2 % of total calories from fat; D3) diets to change their gut microbiomes. Subsequently, faecal microbiome was transplanted (FMT) into mice treated with high cholesterol diet contained 2 % cholesterol, followed by AC or non-AC (sterile tap water, STW) treatments. Control groups with normal (NC) and high-cholesterol diets (PC) were prepared for both AC and STW treatment. All experimental groups were subjected to serum and liver cholesterol, cholesterol metabolism-related (CMR) gene expression, and intestinal microbiome analyses. D3-FMT mice showed the most significant enhancements in cholesterol ratio and decreased hepatic cholesterol levels with AC treatment. Moreover, upregulation of the Cyp7a1 gene expression was observed in this group. Furthermore, the intestinal microbiome analysis indicated higher abundances of BSH-producing Eubacterium, Bifidobacterium, and Parabacteroides in the D3-FMT + AC group compare to others, potentially contributing to increased bile acid synthesis.

Sustainable valorization of waste plastic into nanostructured materials for environmental, energy, catalytic and biomedical applications: A review

The extensive production and utilization of plastic products are inevitable in the current scenario. However, the non-degradable nature of waste plastic generated after use poses a grave concern. Comprehensive efforts are being made to find viable technological solutions to manage the escalating challenge of waste plastic. This review focuses on the progress made in transformation of waste plastic into value-added nanomaterials. An overview is provided of the waste plastic issue on a global level and its ecological impacts. Currently established methodologies for waste plastic management are examined, along with their limitations. Subsequently, state-of-the-art techniques for converting waste plastic into nanostructured materials are presented, with a critical evaluation of their distinct merits and demerits. Several demonstrated technologies and case studies are discussed regarding the utilization of these nanomaterials in diverse applications, including environmental remediation, energy production and storage, catalytic processes, sensors, drug delivery, bioimaging, regenerative medicine and advanced packaging materials. Moreover, challenges and prospects in the commercial level production of waste plastic-derived nanomaterials and their adoption for industrial and practical usage are highlighted. Overall, this work underscores the potential of transforming waste plastic into nanostructured materials for multifaceted applications. The valorization approach presented here offers an integration of waste plastic management and sustainable nanotechnology. The development of such technologies should pave the way toward a circular economy and the attainment of sustainable development goals.

The History of US Menstrual Health, School Nurses, and the Future of Menstrual Health Equity.

In the United States, adolescents suffer from inadequate menstrual health, meaning that adolescents are unprepared for menarche, lack the practical resources they need to comfortably and confidently manage menstruation, and receive inadequate health education and care for menstrual pain and disorders. In this article, we provide a historical analysis of the role of school nurses in addressing menstruation from the early 20th century up to the present day. We contextualize the current realities of school nursing and menstrual health education and clinical support. We argue that the decentralized US school system, a cultural aversion to open discussion about menstruation, and the outsized influence of commercial menstrual product manufacturers have hampered the ability of school nurses to deliver menstrual health education along with menstrual health support. Finally, we discuss implications for today's schooling experiences as well as recommendations for how to support school nurses in aligning our national approach to menstrual health toward the public health perspective of menstrual equity. (Am J Public Health. 2024;114(9):903-908. https://doi.org/10.2105/AJPH.2024.307705).

Eco-friendly one-step egg white gel preparation for sensitive detection of 13 trichothecenes in oats using UHPLC-MS/MS.

Oat products have gained widespread recognition as a health food due to their rich and balanced nutritional profile and convenience. However, the unique matrix composition of oats, which differs significantly from other cereals, presents specific challenges for mycotoxin analysis. This study presents an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method enhanced with an innovative egg white gel pretreatment for the simultaneous analysis of 13 regulated and unregulated trichothecenes in oats. The method demonstrated excellent performance with high accuracy (> 87.5%), repeatability (< 5.7%), and reproducibility (< 8.1%). Analysis of 100 commercial oat products revealed a concerning detection rate (78%) for at least one of the 11 trichothecenes investigated. Notably, deoxynivalenol, exceeding the standard limit in 2% of samples, exhibited the highest detection rate (62%). Additionally, concerning co-occurrence patterns and positive correlations were observed, highlighting potential synergistic effects. The first-time detection of unregulated mycotoxins (T-2 triol, 4,15-diacetoxyscirpenol, 15-acetoxyscirpenol, and neosolaniol) underscores the need for comprehensive monitoring. This method, while developed for oats, shows potential for broader application to other cereals, though further investigation and confirmation are necessary. These findings suggest a potentially underestimated risk of trichothecenes in oats, necessitating continuous monitoring to ensure consumer safety.

Maximizing vanillin loading into paper substrate and its effect on the paper mechanical properties

Abstract Vanillin (4-hydroxy-3-methoxybenzaldehyde) is one of the natural food flavouring agents, can be used as an additive in paper packaging. This study aims to understand the suitable solvent to use to achieve the highest vanilla loading into the paper substrate, and its effect on the paper mechanical properties. A total of three types of commercial vanilla flavor products (A, B, and C) in an oil form were diluted to make concentration of 5% (by weight) solution in isopropyl alcohol (IPA) or ethanol (EtOH), to immerse 29 gsm paper substrate. The vanillin content of the solution A, B and C in EtOH were 0.95, 1.17, and 1.14% w/v, whereas in IPA were 0.75, 0.81, and 0.69% w/v, respectively. The vanillin content absorbed in paper after immersion was determined from the assay of the samples obtained from an extraction process using water. It was found out that the vanillin concentration in the extract from the paper immersed with flavor A, B and C in EtOH and IPA were 0.05, 0.04, 0.07% w/v, and 0.09, 0.06, 0.03% w/v, respectively. It was also found out that the addition of vanilla had affected the mechanical properties of the paper (tensile strength, TEA and stretch). Compared to the paper without vanilla flavor, paper with flavor A in both solvents have 5% lower of all mechanical properties measured. The papers with flavor B in EtOH showed 10% increase in the mechanical properties, while in IPA showed 5% increase in all properties measured. Different impacts on properties were observed on flavor C. The tensile strength, stretch, and TEA increased by 9, 20, and 50% respectively.

P19-73 Commercial mushroom products as a source of biogenic amines

P19-73 Commercial mushroom products as a source of biogenic amines

Quality risk management and data integrity in R&D laboratories supporting CMC lifecycle of biological products

The development of pharmaceutical products is the critical bridge that moves a potential new medicine from academic discovery to applied treatment of patients. It translates an idea for a new drug to bench-level research on how it can be manufactured, formulated, characterized and controlled for use in non-clinical and early clinical trials.From pre-clinical R&D discovery work through the commercial launch, substantial R&D CMC data is generated to develop and optimize cGMP manufacturing and testing operations, while also supporting product comparability, elucidating product / impurity structures, assessing critical quality attributes, developing the drug delivery mode, and developing the product formulation for long-term stability. Significant R&D CMC work continues post-approval to support continuous improvement and market expansion of the commercial product. These activities are crucial elements of Product Lifecycle Management, and taken together, they comprise Pharmaceutical Quality or Chemistry, Manufacturing and Controls (CMC). The objective of this paper is to mitigate the regulatory ambiguity of R&D quality systems with practical, risk-based examples and recommendations when conducting supportive CMC studies for biological products.Making sound strategic CMC decisions under any circumstances assumes data from R&D studies are reliable, traceable, and complete. While there are specific regulatory guidelines on phase-appropriate cGMP activities, none exist for quality practices in R&D CMC laboratories conducting non-cGMP studies. Hindsight is not the time to discover that R&D studies lack key elements that would otherwise have allowed the data to be directly presented to regulators, if needed. There is a strong prospective business interest in protecting considerable investments made for CMC R&D studies. Therefore, establishment of a robust and stage-appropriate R&D laboratory quality system is essential for companies seeking to capitalize on prior knowledge, protect investments, and be prepared for accelerated approval pathways.

Growth-Promoting Bacteria and Salicylic Acid Improve Essential Oil, Flower Yield, And Compatible Osmolyte Content of Common Yarrow (Achillea millefolium L.) Under Drought Stress

Drought is one of the major constraints on agricultural productivity in arid and semiarid regions. Coping with drought stress involves several adaptations and mitigation strategies such as the use of plant-growth promoting bacteria and salicylic acid, which was examined in this study under two levels of water regimes (well-watered as control and drought stress) in southwest Iran in 2018 and 2020 on common yarrow (Achillea millefolium). Catalase levels significantly increased in response to drought stress and salicylic acid application. Salicylic acid and combined application treatment of both bacteria also resulted in a significant increase in proline compared with the control. Different bacteria strains also resulted in a significant increase in peroxidase and superoxide dismutase when compared with the control. Drought stress, salicylic acid application, and different bacteria strains significantly reduced total protein. The use of salicylic acid and the combined application of both bacteria significantly increased the percentage of essential oil compared with the control. Moreover, 41 compounds were isolated, accounting for 92.08% of the essential oil, with more than 90.00% being volatile terpenes. The main components of yarrow essential oil were borneol (16.79%), flanderin (15.92%), cineole (14.32%), camphor (9.77%), and alpha-pinene (3.44%). In general, growth-promoting bacteria and salicylic acid application constitute an advantageous management practice for the commercial production of Achillea millefolium, increasing the nutraceutical and medicinal values of this species.

Is it possible to obtain substitutes for human milk oligosaccharides from bovine milk, goat milk, or other mammal milks?

Considering the current level of chemical and biological synthesis technology, it was a sensible selection to obtain milk oligosaccharides (MOs) from other mammals as the potential substitute for human MOs (HMOs) that possessed various structural features in the infant formula. Through a comprehensive analysis of the content, structure, and function of MOs in six distinct varieties of mammal milk, it has been shown that goat milk was the most suitable material for the preparation as a human milk substitute. Goat MOs (GMOs) had a relatively high content and diverse structural features compared to those found in other mammalian milks. The concentration of GMOs in colostrum ranged from 60 to 350mg/L, whereas in mature milk, it ranged from 200 to 24,00mg/L. The acidic oligosaccharides in goat milk have attracted considerable attention due to their closeness in acidic content and structural diversity with HMOs. Simultaneously, it was discovered that some structures, like N-glycolylneuraminic acid, were found to have a certain content in GMOs and served essential functional properties. Moreover, studies focused on the extraction of MOs from goat milk indicated that the production of GMOs on an industrial scale was viable. Furthermore, it is imperative to do further study on GMOs to enhance the preparation process, discover of new MOs structures and bioactivity evaluation, which will contribute to the development of both the commercial production of MOs and the goat milk industry.

Efficiency of handmade attractants in fruit fly control

Fruit flies (Diptera: Tephritidae) are considered the main pests of fruit crops worldwide, and using traps and attractants are useful tools for their effective monitoring and control. The study's main objective was to evaluate the capacity to capture fruit flies with non-commercially produced attractants and use the Lin and Binns method to evaluate their performance. Five food attractants were evaluated to capture the genera Anastrepha and Ceratitis. The study was carried out in five municipalities of Táchira State, Venezuela, during two semesters, using a completely randomized design with four replications. The experimental unit was represented by a JD EuGo 97 trap, baited with 300 mL of the respective attractant solution. The attractants did not perform equally in all environments, while no variation was observed in the level of trap capture between the two semesters studied. Anastrepha fraterculus was the only species collected at all altitudinal levels, suggesting an adequate capacity to adapt to different environments. The commercial product PedGo plus was the most effective attractant for fly trapping, and it may be an appropriate alternative for use by fruit growers in the control of this pest. Although it was the most efficient attractant in most of the environments studied, molasses + urea proved to be an alternative to replace PedGo plus and Nulure.

Biopriming of corn seeds based on Bacillus subtilis under different storage periods

Corn crop (Zea mays L.) is one of the pillars of Brazilian agriculture, with a wide cultivation area and high productivity; Brazil is the world's second largest exporter of this cereal. For this reason, the aim of the present study was to evaluate the viability of biopriming of corn seeds with a product based on Bacillus subtilis, at different concentrations, up to 120 days of storage. The research was carried out in the seed technology laboratory of the State University of Londrina (UEL), using the commercial corn seeds Balu 366. The experimental design was completely randomized, with four replications, in a 7 × 5 factorial scheme, with seven concentrations of the commercial product based on Bacillus subtilis (Serenade) for seed biopriming (0.0, 2.5, 5.0, 7.5, 10.0, 12.5, and 15.0 %) and five storage periods in a cold chamber (0, 30, 60, 90, and 120 days). The evaluation of the physiological quality of the seeds occurred through germination, cold and seedling length tests. The variables analyzed were first germination count, germination percentage, vigor from the cold test, shoot length, length of the longest root, shoot dry mass, and root dry mass. For the statistical analysis, adjustments of regression models with the best fit were made. The biopriming of seeds proved to be an advantageous practice to enhance the physiological quality of the seeds under storage; it should be carried out at a concentration of 2.5 %, due to its economic viability, considering its efficiency equivalent to the highest concentrations. The biopriming of seeds proved to be a viable practice for seed storage for up to 60 days.

Development of GeSn epitaxial films with strong direct bandgap luminescence in the mid-wave infrared region using a commercial chemical vapor deposition reactor

Germanium tin (GeSn) is a material of interest for electronic and photonic device applications, but its development and commercialization have been limited by material quality issues and lack of availability from epitaxy suppliers. In this paper, we report initial studies in optimizing GeSn films deposited on a Ge buffer layer grown on 200-mm diameter silicon (Si) substrates with an ASM Epsilon 2000 chemical vapor deposition reactor designed for commercial production. Using a single-step growth process, a Sn content up to 22% near the surface of a GeSn film was achieved due to the increase in Sn incorporation via strain relaxation. A two-step growth process resulted in a bilayer structure with a nearly 100% relaxation on the first layer, followed by a higher quality GeSn layer with 18% Sn as evident by a high photoluminescence intensity emitting in the mid-wave infrared region at 3.2 μm at 20 K.

Plant extracts and commercial products for controlling black spot and inducing resistance in rose bushes

Rose is one of the most appreciated flowers in the world; however, fungal diseases can affect its production, decreasing its commercial value. Thus, the objective of this work was to assess the potential of extracts of medicinal plants and commercial products based on plant extracts, plant oils, and potassium phosphite to control black spot (Diplocarpon rosae) in roses by evaluating the activation of plant defense mechanisms. The treatments consisted of using crude aqueous extract (CAE) of Rosmarinus officinalis leaves; CAE of Equisetum arvense stems; CAE of Moringa oleifera seeds; commercial product (CP) based on fermented plant extracts; CP based on potassium phosphite; CP based on plant oils; CP based on citrus mass; and fungicide. Severity of black spot disease, and yield, biometry, chlorophyll contents, and enzyme activity of peroxidase, catalase, and polyphenoloxidase in rose plants were evaluated. The treatments presented no difference from the control (water) for disease incidence and severity and yield. However, the longest stem was found in rose plants in the treatment with potassium phosphite-based commercial product, whereas the thickest stems were found in the three treatments with commercial products. The highest enzyme specific activities of peroxidase, catalase, and polyphenoloxidase were found in plants treated with potassium phosphite, CAE of M. oleifera, and CAE of R. officinalis. The results found showed that the tested natural products did not control black spot in rose bushes, despite their positive effect on plant physiology.

Applications of an Electrochemical Sensory Array Coupled with Chemometric Modeling for Electronic Cigarettes.

This study investigates the application of an eNose (electrochemical sensory array) device as a rapid and cost-effective screening tool to detect increasingly prevalent counterfeit electronic cigarettes, and those to which potentially hazardous excipients such as vitamin E acetate (VEA) have been added, without the need to generate and test the aerosol such products are intended to emit. A portable, in-field screening tool would also allow government officials to swiftly identify adulterated electronic cigarette e-liquids containing illicit flavorings such as menthol. Our approach involved developing canonical discriminant analysis (CDA) models to differentiate formulation components, including e-liquid bases and nicotine, which the eNose accurately identified. Additionally, models were created using e-liquid bases adulterated with menthol and VEA. The eNose and CDA model correctly identified menthol-containing e-liquids in all instances but were only able to identify VEA in 66.6% of cases. To demonstrate the applicability of this model to a commercial product, a Virginia Tobacco JUUL product was adulterated with menthol and VEA. A CDA model was constructed and, when tested against the prediction set, it was able to identify samples adulterated with menthol 91.6% of the time and those containing VEA in 75% of attempts. To test the ability of this approach to distinguish commercial e-liquid brands, a model using six commercial products was generated and tested against randomized samples on the same day as model creation. The CDA model had a cross-validation of 91.7%. When randomized samples were presented to the model on different days, cross-validation fell to 41.7%, suggesting that interday variability was problematic. However, a subsequently developed support vector machine (SVM) identification algorithm was deployed, increasing the cross-validation to 84.7%. A prediction set was challenged against this model, yielding an accuracy of 94.4%. Altered Elf Bar and Hyde IQ formulations were used to simulate counterfeit products, and in all cases, the brand identification model did not classify these samples as their reference product. This study demonstrates the eNose's capability to distinguish between various odors emitted from e-liquids, highlighting its potential to identify counterfeit and adulterated products in the field without the need to generate and test the aerosol emitted from an electronic cigarette.

Introducing Saffron and a few medicinal and high value plants for small farms and water deficit conditions of northern New Mexico

This document reviews the potential of cultivating saffron and other high-value medicinal and aromatic plants such as lavender, rosemary, thyme, chamomile, and Damask rose on small farms in northern New Mexico, particularly under water deficit conditions. Medicinal and aromatic plants, rich in secondary metabolites, are crucial to various industries including pharmaceuticals, cosmetics, and food. With an increasing global market for herbal products and a rising interest in traditional medicine, these plants offer economic benefits and sustainability for small-scale farmers. Northern New Mexico's diverse climate and soil conditions are suitable for growing a wide range of these plants, which are more lucrative and adaptable to dryland farming compared to traditional crops. Saffron (Crocus sativus), the most expensive herb globally, thrives in arid and semi-arid conditions and requires minimal resources. Its cultivation can enhance farm profitability through the integration of summer vegetable production. Lavender (Lavandula spp.), another high-value crop, is well-suited for the region and offers multiple uses in essential oils and personal care products. Rosemary (Rosmarinus officinalis) and thyme (Thymus vulgaris) are also highlighted for their drought tolerance and medicinal properties. Chamomile (Matricaria chamomilla) and Damask rose (Rosa damascena) are noted for their medicinal uses and potential in commercial production. The document underscores the importance of proper soil and water management, organic farming practices, and the role of secondary metabolites in enhancing crop quality under stressful conditions. Intercropping and the use of farm residues for soil conservation are also discussed. The review concludes that these high-value crops can diversify farm income, improve land use efficiency, and offer sustainable agricultural solutions for water-scarce regions in northern New Mexico.

Conjugation of chitosan oligosaccharide to papain for controllable anti-felting finishing of wool textiles

The application of protease as a biocatalyst for the anti-felting finishing of wool has been widely explored. However, commercial proteases tend to hydrolyze the non-keratinized proteins in the cell membrane complex (CMC) layer while removing wool scales, resulting in excessive hydrolysis and unacceptable fiber damage. Herein, chitosan oligosaccharide (COS) was covalently grafted to papain using carbodiimide/N-hydroxysuccinimide (EDC/NHS) to prepare a less-destructive modified protease of Pap-COS. Subsequently, one-bath anti-felting finishing of wool was carried out using reducing agent and Pap-COS, aiming at achieving low shrinkage and reduced strength loss. The results reveal that Pap-COS achieved a larger particle size than native papain, accompanied by a prolongation of its half-life and a decrease in the thermal inactivation rate, demonstrating improved stability. Under the same incubation conditions, the felting shrinkage of wool fabrics treated with papain and Pap-COS was similar (approximately 5 %). In contrast, native papain caused a 20.82 % decrease in fabric strength, while Pap-COS resulted in a substantially lower decrease (8.10 %), meeting the requirements of commercial woolen products. Furthermore, the dyeing performance and wearability of the Pap-COS-treated wool fabrics were also dramatically improved. The present work provides an alternative for eco-friendly shrink-resistant finishing of woolen textiles in wool industry.

ICT adoption, commercial orientation and productivity: Understanding the digital divide in Rural China

This study investigates the impact of Chinese smallholders’ adoption of Information and Communication Technologies—the use of smartphones and computers connected to the internet—on their commercial orientation, land, and labor productivity. Commercial orientation is the share of farm output for sales in the market. We used a control function approach and a selectivity-corrected model. The study uses national survey data from rural sample households, the China Household Database, and the China Household Finance Survey and Research Center. Findings reveal that the adoption of information and communication technologies by Chinese farmers increased the commercial orientation of farming. Furthermore, adopting information and communication technologies increases land and labor productivity by about 21.3 % and 28.2 %, respectively. Farm households’ commercial orientation improved labor productivity by about 35.9 %. Heterogeneity indicates that the adoption of information and communication technologies has a more significant effect on improving productivity for young household heads and small farmers. Policymakers should establish information and communication technologies training programs, develop digital infrastructure, and promote smallholder commercial production to increase agricultural productivity.

Medidas de control de olores para un tanque homogeneizador de una planta de tratamiento de aguas residuales en Costa Rica

[Objective] This study aimed to evaluate the short-term response of four different odor control measures in a homogenization tank of a university Wastewater Treatment Plant (WWTP). [Methodology] The following were implemented: aeration, adding lime, adding iron sulfate, and adding the commercial product BiOWiSH® Odor over four hydraulic retention times. The effluent and the unit were monitored during each period, using a baseline to compare the measures. Hydrogen potential, oxidation-reduction potential, dissolved oxygen, and dissolved sulfide concentration were measured in triplicate every 30 minutes. An odor perception survey was administered to WWTP visitors to evaluate intensity, offensiveness, and character (15 surveys per monitoring period). Differences were determined between control measures and the baseline using Student’s t-tests (quantitative data) and Mood’s median tests (qualitative data). Minitab 2019 was used with a 95% confidence level. [Results] All control measures significantly reduced the dissolved sulfide concentration in the unit. Aeration prevented oxygen depletion, reductive regime, and acid fermentation, which favored the oxidation of odorants. Aeration was also the only studied control measure that reduced odor intensity and offensiveness. [Conclusions] Applying aeration changed unit behavior and odor perception, which confirms its effectiveness, while adding iron sulfate, lime, and BiOWiSH® did not significantly change odor perception.

Vaccinated Atlantic salmon (Salmo salar L.) maintain a specific antibody response throughout the seasonal fluctuations of a full commercial production cycle in sea: a case study

Vaccination is a critical prophylactic measure to prevent disease outbreaks in Atlantic salmon (Salmo salar L.) and multivalent oil-adjuvanted vaccines administered intraperitoneally (IP) are widely used for this purpose. The Atlantic salmon in this observational study were sampled during commercial production in a salmon farm in Northern Norway. Fish were sampled in the hatchery prior to vaccination, and further every 6 weeks after sea transfer up until slaughter. The key findings revealed that Atlantic salmon vaccinated IP with a commercial multivalent oil-based vaccine developed specific and long-lasting antibody responses against A. salmonicida, V. anguillarum, Y. ruckeri, and IPNV for up to 20 months post-vaccination. However, there was variation in the measured specific antibody response to the different antigens. Notably, the antibody responses against A. salmonicida and V. anguillarum antigens were higher and more prolonged compared to the antibody responses against Y. ruckeri and IPNV antigens. Histological examination showed presence of vaccine-induced granulomas in the pancreas at the injection site for up to 50 weeks post-vaccination and V. anguillarum antigens were found in the granulomas up to 44 weeks post-vaccination. There were also individual IgM-positive cells present in these vaccine-induced granulomas, and qPCR confirmed increased gene expression of membrane-bound and secretory IgM up to 37 weeks post-vaccination. Altogether the qPCR data of membrane and secretory IgM suggests local production of IgM in the vaccine-induced granulomas, while the head kidney might be responsible for the long-term secretion of IgM. Neither the low temperature the fish experienced for two winter seasons (below 5 °C) nor the seasonal changes in photoperiod affected the vaccine-induced specific antibody responses.

Effect of ear cleaning on treatment outcome for canine otitis externa.

Ear cleaning is often recommended for management of canine otitis externa (OE). Few invivo studies evaluate how ear cleaning affects treatment outcome. To determine the effect of ear cleaning on canine OE by comparing treatment outcome in cleaned versus noncleaned ears. Twenty-three client-owned dogs with ceruminous or purulent OE. Forty ears were randomised to one of two groups: 20 ears were wiped with dry or saline-moistened gauze (grp1), and 20 external ear canals received manual cleaning (grp2) with a commercial product. One millilitre of a commercial otic suspension containing hydrocortisone aceponate, miconazole nitrate and gentamicin sulfate was applied to affected ears every 24 h for five days. Cytological scores, modified otitis index score (OTIS3), pruritus Visual Analog Scale (PVAS), modified PVAS and client questionnaire were compared on Day (D)0 and D7. There were no statistical differences between cytological scores, modified OTIS3, PVAS, modified PVAS or client assessments between groups at D7. Both groups had significant reduction in all treatment parameters from D0 to D7 with the exception of cytological rod scores that were only significantly decreased in cleaned ears. There was a significant difference in OTIS3 before and after cleaning on D0. Both groups achieved successful outcome regardless of cleaning. Ear cleaning may be more important when rod-shaped bacteria are present.