Commercial Plant Research Articles

Growth Responses to two Commercial Plant Growth Retardants with Different Concentration in Potted Geranium

Background and objective: The aim of this study was to elucidate the effect of varying treatment concentrations of two different plant growth retardants, acting at different inhibition positions during the biosynthetic process, on the growth characteristics of geranium (<i>Pelargonium</i> × <i>hortorum</i>) and then to provide essential data to improve the quality of pot plant production.Methods: Aqueous solutions of diniconazole (Binnari) and daminozide (B-9) at concentrations of 0 (distilled water), 50, 100, 200, and 400 mg⋅L<sup>-1</sup> were prepared for the geranium 'Ringo 2000TM Deep Red'. These solutions were applied by foliar spraying twice at 10-day intervals. Growth characteristics such as plant height, leaf size and relative chlorophyll content (SPAD) were then assessed.Results: When Binnari was applied at concentrations of 0, 50, 100, 200, and 400 mg⋅L<sup>-1</sup> , the corresponding plant heights were 29.5, 23.8, 24.1, 23.0, and 18.4 cm, respectively. There was a statistically significant decrease in plant height with increasing concentration. Conversely, for B-9, plant height was significantly reduced to 26.7 cm only at the 400 mg⋅L<sup>-1</sup> concentration, indicating a less pronounced inhibitory effect compared to Binnari. Leaf width showed a significant decreasing trend with increasing Binnari application concentrations and was 4.6, 4.7, 4.1, 4.2, and 3.9 cm respectively. However, there was no significant difference due to the B-9 treatment. Chlorophyll content increased with increasing concentrations of both retardants.Conclusion: In conclusion, Binnari showed a greater inhibitory effect on geranium growth compared to B-9. The application of Binnari at 400 mg⋅L<sup>-1</sup> resulted in the greatest increase in the ornamental value of the geranium. Furthermore, diniconazole acts during the early stage before the splitting of the GA<sub>1</sub> and GA<sub>4</sub> pathways, thus inhibiting the biosynthesis of both active gibberellins. In contrast, daminozide inhibits the step just before GA<sub>1</sub> biosynthesis. It is concluded that GA<sub>4</sub> serves as the dominant active gibberellin in geranium plants.

Evaluation of In-vitro Anticandidal Activity of 99 Different Commercial Plant Extract, Fixed and Essential Oils against Vaginal Candida albicans Isolates

Plant extracts (PE), fixed oils (FO) and essential oils (EO) are used in traditional medicine to treat various diseases. This study evaluated the anticandidal activity of 100 different commercially available PEs, FOs, and EOs against 19 Candida albicans vaginal isolates and C. albicans ATCC 10231. It was determined that 20 EOs and FOs had anticandidal activity. Piper nigrum FO, pine turpentine EO, pine tar EO, and Eugenia caryophyllata EO showed the highest anticandidal activity. The minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) values of these FO and EOs were between 0.125 and 2 µL mL-1. The volatile components of these FOs and EOs were determined by GC-MS analysis. There were six components in E. caryophyllata EO, 38 in Pine turpentine EO, 39 in P. nigrum FO, and 119 in Pine tar EO. In conclusion, this FOs and EOs can be used to treat vulvovaginal candidiasis.

71 Effects of Late Gestational Nutrient Restriction on Calf pre-Weaning Growth, Backgrounding and Feedlot Performance, and Carcass Composition

Abstract Fall-calving Hereford-SimAngus heifers [single-sired; BW: 451 ± 28 (SD) kg; BCS: 5.4 ± 0.7] bred to a single sire were individually fed 100% (control; CON; n = 12) or 70% (nutrient restricted; NR; n = 13) of NASEM metabolizable energy and metabolizable protein requirements for maintenance, pregnancy, and growth from day 160 of gestation to calving. Post-calving, all females were fed chopped tall-fescue hay supplemented to meet lactational nutrient requirements in Calan gates to constrain diets of calves to milk only until day 147, then pen-fed in drylots until weaning at day 242 ± 3 (SD). Post-weaning, calves were fed a tall fescue hay-based backgrounding diet in the same large drylots for 53 ± 12 d, then moved to partially covered pens with GrowSafe feeding systems in 2 groups and fed a corn-based feedlot diet (formulated to be 18.3% CP, 1.21 Mcal NEg/kg, DM basis; 89% DM) for 255 ± 3 d (similar days on feed rather than carcass endpoint). Cattle were harvested in a commercial plant, and camera carcass grading was performed. Data were analyzed with maternal treatment, calving date, and calf sex (when P < 0.25) as fixed effects; feedlot group was included for feedlot and carcass measures. We previously reported that birth weight was not affected (P = 0.72), but NR decreased (P < 0.04) milk production by 15%. Calves born to NR dams had reduced ADG from birth to day 147 (P = 0.02), day 147 to weaning (P = 0.08), and birth to weaning (P = 0.003), resulting in NR calves having 13% lighter (P = 0.004) weaning weight. Backgrounding ADG was not affected (P = 0.80) by maternal treatment; thus, feedlot entry BW was 11% less (P = 0.009) in NR calves. On day 28, 56, 84, and 112 on feed, NR calves weighed less (P ≤ 0.09) than control. Final BW was not affected (P = 0.91) by maternal treatment. Feedlot ADG was greater for NR calves from day 168 to slaughter (P = 0.02) and the overall feeding period (P = 0.03), but not before day 168 on feed (P ≥ 0.22). Calves born to NR dams had less (P = 0.02) dressing percentage, but improved (P = 0.02) yield grade and backfat thickness. Hot carcass weight, ribeye area, marbling score, and KPH were not affected (P ≥ 0.26) by maternal treatment. Percent prime, yield grade ≤ 2, and yield grade ≥ 4 were not affected (P ≥ 0.29) by maternal treatment. Overall, late gestational nutrient restriction decreased growth pre-weaning but increased late finishing growth, without affecting backgrounding or early feedlot growth. These cattle had leaner carcasses after similar days on feed, suggesting that late gestational nutrient restriction altered growth patterns or body composition.

PSIV-1 Nutrient Intervention During Gilt Development Leads to Effects on Progeny Growth Responses

Abstract To elucidate the effect of early life dietary nutrient level and vitamin D source during gilt development on progeny growth performance, equal number of weaning age piglets (n = 72) were selected from each of 4 sow treatments group. All pigs were fed a common 3,000 ppm zinc diet for first two weeks postweaning to allow for accommodating the weaning process. At the beginning of week 3, pigs were sorted by sow parity and BW within gender and stratified based on maternal dietary treatments. Dietary treatments during gilt development period included two levels of dietary nutrients (Adeq: optimal growing diet that exceed NRC 2012 recommendation or Low: moderate slow growing diet that SID lys and ME levels were gradually reduced by 0.03% and 0 kcal/kg, 0.15% and 146 kcal/kg, and 0.15% and 150 kcal/kg, respectively) and two sources of vitamin D [StD: 1653 IU cholecalciferol/kg or HyD: 500 IU cholecalciferol/kg + 50 mcg calcifediol (ROVIMIX HY.D DSM Nutritional Products North America)] during development phase 1 (23 to 57 kg), 2 (57 to 91 kg), and 3 (91 until breeding). Dietary nutrient level treatment was withdrawn during gestation and lactation stages, while sows remained on their vitamin D treatments. Progeny were fed with a 6-phase feeding regimen as nursery phase 3 (14 d) and 4 (14 d), grower phase 1 (28 d) and 2 (28 d), and finisher phase 1 (28 d) and 2 (28 d). All pigs were transferred to a commercial processing plant where carcass characteristics were measured. Data were analyzed using Mixed procedure of SAS (Cary, NC) with maternal diet nutrient level, vitamin D source and the interaction as fixed effects. Progeny born from sows fed HyD diets had greater ADG in overall grower/finisher phases (P = 0.04) and during the whole trial (P = 0.03). This weight gain improvement increased the final BW, HCW, and yield percentage over StD derived progeny by 3.19 kg, 3.56 kg and 0.11% (P = 0.04, 0.02 and < 0.01), respectively. Dietary nutrient level had limited effect on progeny ADG and BW during the preslaughter period. The maternal vitamin D source did not affect progeny ADFI but overall G:F tended (P = 0.06) to be greater in pigs sourced from dams fed HyD . A maternal vitamin D by nutrient level interaction was observed for ADFI during grower/finisher phases (P = 0.05) and overall (P = 0.07) because progeny from gilts fed a low nutrient level had greater intake than cohorts from gilts fed an adequate nutrient diet, while ADFI was similar for all offspring from gilts fed HyD diets. These results indicate that manipulating nutrients during gilt development, or feeding HyD rather than StD to reproducing sows, can affect growth of their progeny to slaughter.

The Dynamic Accumulation Rules of Chemical Components during the Medicine Formation Period of Angelica sinensis and Chemometric Classifying Analysis for Different Bolting Times Using ATR-FTIR

The dried roots of the perennial herb Angelica sinensis (Oliv.) Diels (AS) are commonly used as medicinal and edible resources. In commercial planting, early bolting and flowering (EB) of ca. 60% in the medicine formation period reduces root yield and quality, becoming a significant bottleneck in agricultural production. In the cultivation process, summer bolting (SB) occurs from June to July, and autumn bolting (AB) occurs in September. The AB root is often mistaken for the AS root due to its similar morphological characteristics. Few studies have involved whether the root of AB could be used as herbal medicine. This study explored and compared the accumulation dynamics of primary and secondary metabolites in AS and EB roots during the vegetative growth stage (from May to September) by light microscopy, ultraviolet spectrometry, and HPLC methods. Under a microscope, the amount of free starch granules and oil chamber in the AS root increased. On the contrary, they decreased further from EB-Jul to EB-Sep. By comparison, the wall of the xylem vessel was slightly thickened and stacked, and the cell walls of parenchyma and root cortex tissue were thickened in the EB root. Early underground bolting reduces soluble sugar, soluble protein, free amino acids, total C element, total N element, ferulic acid, and ligustilide accumulation, accompanied by the lignification of the root during the vegetative growth stage. Furthermore, a total of 55 root samples from different bolting types of AS root (29 samples), SB root (14 samples), and AB root (12 samples) were collected from Gansu Province during the harvesting period (October). The later the bolting occurred, the less difference there was between unbolted and bolted roots in terms of morphological appearance and efficacy components. Fourier transform infrared spectroscopy with the attenuated total reflection mode (ATR-FTIR) provides a “holistic” spectroscopic fingerprinting of all compositions in the tested sample. The ATR-FTIR spectrum of the AB root was similar to that of the AS root. However, the number and location of absorption peaks in the spectra of SB were different, and only one strong absorption peak at 1021 cm−1 was regarded as the characteristic peak of C-O stretching vibration in lignin. The ATR-FTIR spectra can be effectively differentiated based on their various characteristics using orthogonal partial least squares discrimination analysis (OPLS-DA). Results were assessed using multiple statistical techniques, including Spearman’s correlation, principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and OPLS-DA. Among these methods, the ATR-FTIR data demonstrated the most effective outcomes in differentiating between viable and non-viable roots for their application in herbal medicine. Essential substances are ferulic acid and flavonoid, which are much more abundant in the AB root. It provides a material basis for the pharmacological action of the AB roots and a theoretical basis for improving their availability.

Early and long-distance uptake by Eucalyptus grandis of N, K and Ca tracers injected down to a depth of 7 m

Eucalyptus plantations are commonly fertilized at planting to sustain high biomass production over successive rotations in nutrient-poor soils. However, the dynamics of nutrient uptake by tree roots in the early stages of stand development, depending on the lateral distance from the trees and the depth in the soil, are poorly documented. We assessed the potential uptake of N, K and Ca, from 6 to 18 months after planting, by Eucalyptus grandis trees depending on: i) the lateral distance from the tracer injection point and ii) the depth in the soil. At 6 months after planting, we injected 15NO3–, Rb+, Sr2+ and Cs+ tracers at depths of 1, 3, 5, and 7 m, respectively. Recently expanded leaves were then sampled monthly on 16 target E. grandis trees located at lateral distances ranging from 0.75 to 11.1 m from the injection points, and on 4 more distant E. grandis control trees; 15N contents (atom %) and concentrations of Rb, Cs and Sr in the leaves were determined at each sampling date, and compared to those of control trees to detect the dates of tracer uptake for each individual. A LOESS model was applied to account for the temporal trend and inter-tree variability among the control trees. Tracer uptake by a target tree was detected if the measurements exceeded the upper limit of the prediction interval (α = 0.005) generated by the model. Only the 6 target trees located within 2.0 m from the injection point took up the 15NO3– tracer. However, Rb+, Sr2+ and Cs+ tracers were taken up to lateral distances of approx. 11 m from the injection point. Tracer uptake occurred as early as 9, 7, 8 and 9 months after planting for 15NO3–, Rb+, Sr2+ and Cs+, respectively. Four trees located within 2.8 m from the injection point took up the 3 cation tracers, injected between the depths of 3 m and 7 m. All but one of the target trees took up at least one cation tracer. The potential of Eucalyptus trees to take up nutrients early, at long distance and at depth, can be promoted by silvicultural practices enhancing rapid root development after planting, as subsoiling in commercial plantations and large planting holes in smallholder plantations. Our results also suggest that fertilization could be applied only once at planting in Eucalyptus stands established on deep tropical soils, with low risks of nutrient losses by deep drainage.

Modeling the Tokamak Exhaust Processing System in a Commercial Simulator for Process Monitoring Purposes

Nuclear fusion depends on tritium breeding and self-sufficiency. Tritium represents a hazard due to its radioactivity and migration properties. Because of these difficulties, ITER, the largest fusion experiment so far, relies on a conservative static procedure to monitor the tritium inventory. Future commercial fusion plants can avoid operation halts if a dynamic monitoring strategy proves itself valid. Tritium plant models have been developed for this kind of monitoring and analysis task, but sensor accuracy and reliability are an issue still to be addressed, and the path to dynamic monitoring remains unclear. The present work shows the modeling procedure of the Tokamak Exhaust Processing system in a commercial simulator, Aspen HYSYS, to reproduce the inventories, streams, process conditions, and compositions of this subsystem during operation. The model is verified in a steady-state scenario using data from the available literature. A demonstration of such a tritium plant subsystem shows meaningful value for several reasons. First, this process has not been modeled before in commercial dynamic simulators, which are typically used in the process industry. It will also allow new stakeholders to participate in future fusion-related projects. Second, it will play a key role in industry-like tritium process monitoring, in which the new model will act as a digital twin of the plant. Data-driven diagnostics can be fueled by model data, helping engineers to generate additional data that could otherwise be expensive to get directly from the plant. For these reasons, models will represent an essential part of a dynamic monitoring system, necessary for feasible fusion projects.

Sustainability of Renewable Energy Options as Compared to Coal-Fired Power Plants in Pakistan

Though frequent availability of energy is vital for economic growth, using any energy source can have a certain degree of impact on the environment. Pakistan is facing the worst power crisis nowadays. The government of Pakistan has been focused to find energy solutions in fossil fuels. As the world moves towards a clean sustainable direction, coal sources, are cheap at the moment, and new coal-based power plants can be put up quickly with a predictable output, but with the degradation of the environment, Pakistan can utilize sustainable energy resources such as biomass, solar energy, hydropower, and wind power; which are frequently available in Pakistan and can generate environment-friendly power above 40,000 MW. It is necessary to consider these sustainable energy resources, as their prices have been dipping dramatically and it is now cheaper to build new commercial plants based on improved technologies able to generate more electricity. The present paper discusses the insight environment-friendly sustainable energy options available in Pakistan other than coal-fired plants to overcome future energy demand.

Structural divergence and phylogenetic relationships of Ajania (Asteraceae) from plastomes and ETS

BackgroundAjania Poljakov, an Asteraceae family member, grows mostly in Asia’s arid and semi-desert areas and is a significant commercial and decorative plant. Nevertheless, the genus’ classification has been disputed, and the evolutionary connections within the genus have not been thoroughly defined. Hence, we sequenced and analyzed Ajania’s plastid genomes and combined them with ETS data to assess their phylogenetic relationships.ResultsWe obtained a total of six new Ajania plastid genomes and nine ETS sequences. The whole plastome lengths of the six species sampled ranged from 151,002 bp to 151,115 bp, showing conserved structures. Combined with publicly available data from GenBank, we constructed six datasets to reconstruct the phylogenetic relationships, detecting nucleoplasmic clashes. Our results reveal the affinities of Artemisia, Chrysanthemum and Stilpnolepis to Ajania and validate the early taxonomy reclassification. Some of the plastid genes with low phylogenetic information and gene trees with topological differences may have contributed to the ambiguous phylogenetic results of Ajania. There is extensive evolutionary rate heterogeneity in plastid genes. The psbH and ycf2 genes, which are involved in photosynthesis and ATP transport, are under selective pressure. Plastomes from Ajania species diverged, and structural aspects of plastomes may indicate some of the real evolutionary connections. We suggest the ycf1 gene as a viable plastid DNA barcode because it has significant nucleotide diversity and better reflects evolutionary connections.ConclusionOur findings validate the early Ajania taxonomy reclassification and show evolutionary rate heterogeneity, genetic variety, and phylogenetic heterogeneity of plastid genes. This research might provide new insights into the taxonomy and evolution of Ajania, as well as provide useful information for germplasm innovation and genetic enhancement in horticultural species.

A cost-benefit analysis for the management of Gonipterus platensis by comparing chemical and augmentative biological control

Around 1.5 million ha of the Iberian Peninsula is currently occupied by Eucalyptus globulus, the preferred species for commercial plantations due to its fast growth and wood technological properties. However, these economically important plantations are being heavily damaged by Gonipterus platensis (Col: Curculionidae), an invasive species native to Australia which was established in the Peninsula, in the nineties. A classical biological control with the release of the egg parasitoid Anaphes nitens (Hym: Mymaridae) was launched in the Peninsula. Yet, in the colder regions, in the North of the Peninsula, the parasitoid is not efficient enough to control the weevil population. The resulting damage overcomes more than 200 million euros every year.Current control strategies rely on the application of insecticide (acetamiprid) or augmentative biological control (ABC) with yearly releases of A. nitens in spring. However, uncertainties about the best option persist. In this study, chemical and ABC treatments were submitted to a cost-benefit analysis with the aim of determining their viability against the weevil, based on a treatment efficacy and stand productivity trade-off analysis.A simulation tool (3-PG.d) was used to simulate the growth of E. globulus stands, under four defoliation intensity scenarios mimicking the removal of 25, 50, 75 and 100% of the spring new leaves biomass. Each defoliation scenario was combined with three treatments: (i) chemical (acetamiprid), (ii) ABC+ (optimistic approach), and (iii) ABC- (pessimistic approach). For each treatment, several plans of yearly treatments were tested. The Net Present Value (NPV) and the harvested volume were used to compare all treatment alternatives.The best treatment decision, based on a cost-benefit analysis comparing economic results and harvested volume, varied with the intensity of defoliation. Treating was always better than doing nothing, except for the 25% defoliation scenario. ABC+ offered the best economic results, relative to other treatments, for 25, 50 and 75% defoliation scenarios. ABC- was only as good as the chemical treatment for the 25 and 50% defoliations. Under the heaviest weevil attack (100%), applying insecticide was the best alternative, overcoming both ABC treatments. These results show that augmentative biological control was a strategy to consider for this study, and thus, may be a strategy to be considered in the future.The 3-PG.d simulator showed that it can be an asset for forest managers aiming at integrating chemical and/or biological treatments against forest pests in stand management decisions.

Determinants of Smallholders’ Commercial Plantation Establishment: Challenges and Opportunities to Promote Tree-Based Livelihood in Ethiopia

Determinants of Smallholders’ Commercial Plantation Establishment: Challenges and Opportunities to Promote Tree-Based Livelihood in Ethiopia

Ultra-high Performance Liquid Chromatography-Ion Mobility-High-Resolution Mass Spectrometry to Evaluate the Metabolomic Response of Durum Wheat to Sustainable Treatments.

Sustainable agriculture aims at achieving a healthy food production while reducing the use of fertilizers and greenhouse gas emissions using biostimulants and soil amendments. Untargeted metabolomics by ultra-high performance liquid chromatography-ion mobility-high-resolution mass spectrometry, operating in a high-definition MSE mode, was applied to investigate the metabolome of durum wheat in response to sustainable treatments, i.e., the addition of biochar, commercial plant growth promoting microbes, and their combination. Partial least squares-discriminant analysis provided a good discrimination among treatments with sensitivity, specificity, and a non-error rate close to 1. A total of 88 and 45 discriminant compounds having biological, nutritional, and technological implications were tentatively identified in samples grown in 2020 and 2021. The addition of biochar-biostimulants produced the highest up-regulation of lipids and flavonoids, with the glycolipid desaturation being the most impacted pathway, whereas carbohydrates were mostly down-regulated. The findings achieved suggest the safe use of the combined biochar-biostimulant treatment for sustainable wheat cultivation.

Exploring the potential of concentrating solar power technologies for vertical farming in arid regions: The case of Western Iraq

Indoor vertical farming can address future food insecurity by reducing land use and environmental dependence positively mitigating the environmental impacts associated with conventional agriculture. However, its high energy consumption and environmental impact raise concerns. This paper explores the use of Concentrating Solar Power (CSP) to power vertical farming in Iraq's arid regions, using abundant solar resources to reduce environmental impact and energy costs. The study evaluates the feasibility and benefits of integrating CSP technologies into sustainable agricultural practices, considering factors like implementation costs, energy production, and water usage. The study utilized the system advisor model (SAM) to carry out the techno-economic assessment by relocating existing commercial CSP plant in Seville, Spain to six Iraqi locations, following a comprehensive technical site assessment. The energy production level, capacity factor (CF), and levelized cost of electricity (LCOE) of the initial Spanish plant were lower than five of the six selected locations. Haditha and Ar Rutbha cities have the highest annual energy output, 123 GWh and 122 GWh, respectively, surpassing Seville's original plant output, which at 110 GWh performed less than Iraq locations. Next, comparisons are made between conventional farming and vertical farming (VF) cultivation. The study focuses on Romaine lettuce as the benchmark crop due to its prevalence in vertical farms and its suitability for arid climates. VF shows the potential to achieve yields between 41 and 68 kg/m2/y, 63 to 104 times higher than conventional farming in Western Iraq. VF's year-round cultivation with artificial lighting results in 83 times greater energy demands, requiring 25 kWh/kg/y. The findings emphasize the potential of CSP technologies to support sustainable agriculture in Iraq's arid regions. These findings demonstrate the possibility of utilizing solar power for sustainable agriculture in Iraq, offering valuable insights for policymakers and stakeholders seeking cost-effective and energy-efficient solutions.

Effects of thinning on growth performance of teak (Tectona grandis) plantations in Tain II Forest Reserve, Ghana

Although recent interest in commercial teak plantation establishment has soared, knowledge of intensive silvicultural management of residual stands is limited in most parts of West Africa. This study evaluated the effects of thinning regimes on the growth responses and pruning requirements of teak plantations, two years after thinning, in the transition zone of Ghana. The thinning trial was conducted on a 4-year-old stand with initial stocking of 1 111 trees ha−1 in the Tain II Forest Reserve, Ghana. The treatments were four thinning regimes: 50%, 30%, 0% thinning intensities and 50% decrowning, arranged in a completely randomised design with four replicates. Two years after thinning, the treatments were significantly different in diameter at breast height (DBH) (p < 0.00029), total height (p = 0.017) and stem density (p < 0.0002). The 50% thinning intensity had the highest mean tree DBH, volume, height and basal area of 15.57 cm, 0.13 m3, 11.94 m and 0.02 m2 respectively. The lowest was found in the control (no thinning) except height which was least in decrowned plots. Maximum mean annual increment of DBH, total height and volume were respectively 2.00 cm, 1.34 m and 9.9 m3 ha−1 for the heavily thinned stand. The number of epicormic shoots per tree increased with the thinning intensity/regime, hence increasing the pruning requirement. It is concluded that 50% thinning intensity promotes positive growth of residual stand but increases the pruning requirements and possibly the pruning costs. Long-term evaluation of this trial is essential to validate the recommendations from this study.

Critical stage and key natural mortality factors of Hypothenemus hampei in commercial coffee plantations

AbstractThe coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae), is one of the most important coffee pests, and is present in almost all countries producing this crop, causing annual losses of US$350 million. Natural factors regulate H. hampei populations in coffee crops. Ecological life table analysis is a robust tool that allows the estimation of the mortality caused by these factors. Therefore, the main objective of this study was to determine the critical stage and the key mortality factors affecting H. hampei in the field. Data for constructing life tables were collected over 2 years in six coffee plantations in the regions of Paula Cândido and Viçosa, Minas Gerais State, Brazil. The average mortality of H. hampei was 75.34% ± 5.85%. The mortality at each developmental stage was 24.32% ± 2.83% (eggs), 7.29% ± 1.01% (first instar), 11.58% ± 1.46% (second instar), 9.68% ± 1.10% (third instar), 7.45% ± 1.01% (pupa), and 15.02% ± 1.29% (adult; n = 124). The mortality factors observed and quantified in this study were parasitism by Prorops nasuta (Hymenoptera: Bethylidae), predation by ants (Crematogaster spp., Pheidole spp. and Solenopsis spp.), physiological disorders and fungal infection by Beauveria bassiana and Metarhizium anisopliae. The critical mortality stage of H. hampei was the adult stage. The key factors for mortality of H. hampei adults were predatory ants, followed by B. bassiana and malformations (these insects had deformities in the head, wings, legs, or abdomen). This information is essential to conserve the activities of natural enemies and, thus maintain H. hampei natural mortality factors in coffee plantations, reducing damage to the crops and the need for excessive insecticide interventions.

Model based process optimization of an industrial chromatographic process for separation of lactoferrin from bovine milk

Model based process development using predictive mechanistic models is a powerful tool for in-silico downstream process development. It allows to obtain a thorough understanding of the process reducing experimental effort. While in pharma industry, mechanistic modeling becomes more common in the last years, it is rarely applied in food industry. This case study investigates risk ranking and possible optimization of the industrial process of purifying lactoferrin from bovine milk using SP Sepharose Big Beads with a resin particle diameter of 200 µm, based on a minimal number of lab-scale experiments combining traditional scale-down experiments with mechanistic modeling. Depending on the location and season, process water pH and the composition of raw milk can vary, posing a challenge for highly efficient process development.A predictive model based on the general rate model with steric mass action binding, extended for pH dependence, was calibrated to describe the elution behavior of lactoferrin and main impurities. The gained model was evaluated against changes in flow rate, step elution conditions, and higher loading and showed excellent agreement with the observed experimental data. The model was then used to investigate the critical process parameters, such as water pH, conductivity of elution steps, and flow rate, on process performance and purity. It was found that the elution behavior of lactoferrin is relatively consistent over the pH range of 5.5 to 7.6, while the elution behavior of the main impurities varies greatly with elution pH. As a result, a significant loss in lactoferrin is unavoidable to achieve desired purities at pH levels below pH 6.0. Optimal process parameters were identified to reduce water and salt consumption and increase purity, depending on water pH and raw milk composition. The optimal conductivity for impurity removal in a low conductivity elution step was found to be 43 mS/cm, while a conductivity of 95 mS/cm leads to the lowest overall salt usage during lactoferrin elution. Further increasing the conductivity during lactoferrin elution can only slightly lower the elution volume thus can also lead to higher total salt usage. Low flow rates during elution of 0.2 column volume per minute are beneficial compared to higher flow rates of 1 column volume per minute.The, on lab-scale, calibrated model allows predicting elution volume and impurity removal for large-scale experiments in a commercial plant processing over 106 liters of milk per day. The successful model extrapolation was possible without recalibration or detailed knowledge of the manufacturing plant. This study therefore provides a possible pathway for rapid process development of chromatographic purification in the food industries combining traditional scale-down experiments with mechanistic modeling.

Identifying and quantifying a complete set of full-power initiating events during early design stages of high-temperature gas-cooled reactors

Identifying initiating events is the first step in building a probabilistic risk assessment model. The history of commercial nuclear power plant is primarily light-water reactors, hence, a comprehensive set of possible initiating events that covers most available light-water reactors is identified allowing for a frequentist approach to estimating their associated frequencies. However, other technologies of nuclear power plants do not share the same operating experience, hence, there are no comprehensive lists of initiating events for non-light-water reactors. This paper presents an approach that utilizes top-down deductive methodologies, master logic diagrams, and heat balance fault trees; the bottom-up inductive methodology of failure modes and effects analysis; and legacy and contemporary information to identify a complete list of initiating events for the Xe-100 high-temperature gas-cooled reactor (HTGR). Moreover, the paper presents the approach on how to estimate the frequencies using legacy sources along with deterministic analysis and fault trees. Furthermore, the paper presents an approach to estimate uncertainty parameters associated with each of these initiating events using constrained non-informative distributions to account for lack of operating experience. Finally, the paper presents the list of identified initiating events for the Xe-100 along with their frequencies and uncertainty parameters which serves, along with other contemporary sources, as another building block in having a comprehensive set of initiating events for HTGRs.

First report of black rot disease in Eruca vesicaria subsp. sativa caused by Xanthomonas campestris pv. campestris in Belgium.

Eruca vesicaria subsp. sativa (Mill.) Thell. (arugula or rocket) is a leafy vegetable originating from the Mediterranean region primarily being sold in bagged salads. From 2014 to 2017, plants (cv. Montana) exhibiting blackened leaf veins and irregular V-shaped chlorotic to necroic lesions at the leaf margins were observed in commercial greenhouses in Flanders, Belgium (Figure S1A). Symptoms started after harvest of the first cut, indicating that leaf injury favours disease development. By the last cut, infections had spread uniformly across the plots, with symptoms advanced to the point where harvesting was no longer profitable. Excised surface-sterilized necrotic leaf tissue and seeds were homogenized in phosphate buffer (PB), followed by dilution plating on Pseudomonas Agar F containing sucrose. After four days at 28°C, bright yellow round, mucoid, convex Xanthomonas-like colonies were obtained, both from leaves and seeds. For confirmation, DNA was extracted from pure cultures after which a partial fragment of gyrB was amplified and sequenced (Holtappels et al. 2022). Amplicons were trimmed to 530 nucleotides (Genbank ON815895-ON815900) according to Parkinson et al. (2007) and compared with the NCBI database. Strain GBBC 3139 shares 100% sequence identity with Xanthomonas campestris pv. campestris (Xcc) type strain LMG 568 and with RKFB 1361-1364, isolated from arugula in Serbia (Prokić et al. 2022). The other isolates from Belgian rocket - GBBC 3036, 3058, 3077, 3217 and 3236 - all have a gyrB sequence 100% identical to that of Xcc strain ICMP 4013, among others. To determine the genetic relatedness to other pathogenic Xc strains, the genomes of GBBC 3077, 3217, 3236 and 3139 were sequenced using a MinION (Nanopore) and non-clonal sequences were submitted to NCBI (BioProject PRJNA967242). Genomes were compared by calculating Average Nucleotide Identity (ANI). This revealed that the Belgian strains cluster together with Xc isolates originating from Brassica crops and separate from strains identified as Xc pv. barbareae, pv. incanae and pv. raphani (Figure S2A). Their designation as pv. campestris is supported by maximum likelihood clustering of concatenated gyrB-avrBs2 sequences (EPPO, 2021; Figure S2B,C). Finally, pathogenicity was verified on five-week-old rocket 'Pronto' plants grown in a commercial potting mix by cutting the leaves along the midrib with scissors dipped into a suspension of 108 cfu/ml of each strain or PB as control (4 plants/strain). Plants were kept in closed polypropylene boxes for 48 hr to support high humidity and facilitate infection. They were then maintained at 25 ± 2 °C. Lesions like those observed on commercial plants developed on the inoculated leaves within one week (Figure S1B). Bacterial colonies reisolated from symptomatic tissue were identified based on gyrB as the strains used for inoculation, thereby fulfilling Koch's postulates. To the best of our knowledge, this is the first report of black rot disease in arugula caused by Xcc in Belgium. Previously, Xcc on arugula has been reported in Argentina, California and Serbia as well (Romero et al. 2008; Rosenthal et al. 2017; Prokić et al. 2022). Arugula being a minor crop in Belgium, challenged by Xcc infections and strong import competition, many growers have abandoned the sector in recent years. Therefore, this study makes a strong case for early detection of disease symptoms and timely application of relevant management strategies in vulnerable crop settings.

Detection of commercialized plant products derived from new genomic techniques (NGT) - Practical examples and current perspectives

New genomic techniques (NGT) are increasingly applied in plant research and development in many countries due to their simplicity in introducing genetic modifications that alter specific traits. First NGT products are on the market in several countries where no specific regulatory approval is required. In the European Union (EU), however, all NGT derived products are subject to the existing genetically modified organism (GMO) authorization regulations. This nourishes the discussion on the technical and analytical feasibility of detecting, identifying and differentiating plants produced by targeted mutagenesis from conventional breeding products and spontaneous mutations in routine food/feed and seed/grain testing by enforcement laboratories. The anticipated difficulties are currently based mainly on theoretical considerations, particularly with regard to single nucleotide or very limited sequence changes. Against that background, this brief review examines concrete and practical approaches for the detection and identification of market-relevant NGT plants. Four early commercialized NGT plants have been examined as real examples to verify the feasibility of detection method design and development using routinely applied analytical approaches. In addition, we searched for potential new screening approaches and show, how a common and well conserved genetic element used in CRISPR/Cas9 applications may serve as a potential target, if this foreign DNA element is still integrated in the host genome. The conclusions to be drawn from the considered theoretical approaches and the practical examples are described in the context of key differences to the current GMO testing practice, in particular regarding the difficulty to develop methods specifically identifying NGT applications. Finally, we discuss whether the consideration of such factors could facilitate a resolution of the current dilemma between detection and the ability to identify plant NGT events.

Rhizosphere Bacteria Biofertiliser Formulations Improve Lettuce Growth and Yield under Nursery and Field Conditions

Rhizosphere bacteria can provide multiple benefits to plants, including increased nutrient supply, pathogen/disease control, and abiotic stress tolerance, but results from pot trials do not always translate to field conditions. This study tested whether rhizosphere biocontrol bacteria can also provide plant growth promotion and how benefits can be provided at a commercial farm. Commercial lettuce seeds and plants were treated with rhizosphere biocontrol bacteria Bacillus velezensis UQ9000N, B. amyloliquefaciens 33YE, Brevibacillus laterosporus 4YE, and Pseudomonas azotoformans UQ4510An. 33YE increased the head diameter, plant height, and fresh weight of the Green Moon cultivar, while 33YE, UQ4510An, and UQ9000N increased the fresh and dry weight of Liston, a more heat-tolerant cultivar, via a single seed treatment or repeat root treatments under nursery and field conditions across different inoculation schedules and growth stages. Significant growth promotion was also demonstrated when inoculating field plants after transplanting (in particular for 33YE). Applications of these microbial biostimulants to lettuce seeds or plantlets potentially enable earlier transplanting and earlier harvests. Repeat inoculations using irrigation water and long-lasting formulations may further advance the benefits of these biostimulants as microbial biofertilisers for plant growth promotions in the field.