Commercial Formulations Research Articles

Changes in thermal sensitivity of Rhinella arenarum tadpoles (Anura: Bufonidae) exposed to sublethal concentrations of different pesticide fractions (Lorsban® 75WG).

The intensive use of agrochemicals and the rapid increase of global temperatures have modified the thermal conditions of aquatic environments, thus increasing amphibians' vulnerability to global warming and positioning them at great risk. Commercial formulations of chlorpyrifos (COM) are the pesticides most widely used in agricultural activities, with a high toxic potential on amphibians. However, little is known about the separate effects of the active ingredient (CPF) and adjuvants (AD). We studied the thermal sensitivity at different concentrations and pesticide fractions in Rhinella arenarum tadpoles, on thermal tolerance limits (CTmax = Critical thermal maximum and CTmin = Critical thermal minimum), swimming speed (Ss), Optimum temperature (Top), and Thermal breadth 50 (B50). Our results demonstrate that the pesticide active ingredient, the adjuvants, and the commercial formulation of chlorpyrifos differentially impair the thermal sensitivity of R. arenarum tadpoles. The pesticide fractions affected the heat and the cold tolerance (CTmax and CTmin), depending on the concentrations they were exposed to. The locomotor performance (Ss, Top, and B50) of tadpoles also varied among fractions, treatments, and environmental temperatures. In the context of climate change, the outcomes presented are particularly relevant, as mean temperatures are increasing at unprecedented rates, which suggests that tadpoles inhabiting warming and polluted ponds are currently experiencing deleterious conditions. Considering that larval stages of amphibians are the most susceptible to changing environmental conditions and the alarming predictions about environmental temperatures in the future, it is likely that the synergism between high temperatures and pesticide exposure raise the threat of population deletions in the coming years.

Determination of omeprazole via fluorescence-quenching methodology; application to content uniformity testing.

A new, proven, economical spectrofluorimetric approach has been used to determine the proton pump inhibitor omeprazole (OMP). This innovative technique is based on the ability of OMP to quench the native fluorescence of the mercurochrome dye in an acidic (pH3.6) solution. Because it was discovered that quenching is proportional to the drug concentration, this dye was used as a sensor for OMP detection. The fluorescence intensity was measured at 518/540 nm, and its linear response ranged from 0.2-10.0μg/mL with a linear coefficient of 0.9999. The computation yielded a limit of quantification (LOQ) of 0.20 μg/mL and a limit of detection (LOD) of 0.07 μg/mL. Every circumstance and element impacting the reaction product was examined in detail. Pharmacopeial standards carried out the validation. The approved method investigated several commercial preparations and formulations, and the results were favorably compared with those provided by a reference method. According to United States Pharmacopeia (USP) rules, content consistency for two distinct formulations was evaluated.

Ecological Implications on Aquatic Food Webs Due to Effects of Pesticides on Invertebrate Predators in a Neotropical Region.

Predation presents specific behavioral characteristics for each species, and the interaction between prey and predator influences the structuring of the food web. Concerning insects, predation can be affected in different ways, such as exposure to chemical stressors, e.g., pesticides. Therefore, analyses were carried out of the effects of exposure to insecticide fipronil and the herbicide 2,4-D on predation, parameters of food selectivity, and the swimming behavior of two neotropical predatory aquatic insects of the families Belostomatidae (giant water bugs) and larvae of Libellulidae (dragonfly). These predatory insects were exposed for 24 h to a commercial formulation of the chlorophenoxy herbicide, 2,4-D at nominal concentrations of 200, 300, 700, and 1400μgL-1, and to a commercial formulation of the phenylpyrazole insecticide, fipronil at nominal concentrations of 10, 70, 140, and 250µgL-1. In a control treatment, the insects were placed in clean, unspiked water. At the end of the exposure, the maximum swimming speed of the predators was evaluated. Afterward, the predators were placed in clean water in a shared environment for 24 h with several prey species, including the cladoceran Ceriodaphnia silvestrii, larvae of the insect Chironomus sancticaroli, the amphipod Hyalella meinerti, the ostracod Strandesia trispinosa, and the oligochaete Allonais inaequalis for 24 h. After this period, the consumed prey was counted. The results reveal that predators from both families changed prey consumption compared with organisms from the control treatment, marked by a decrease after exposure to fipronil and an increase in consumption caused by 2,4-D. In addition, there were changes in the food preferences of both predators, especially when exposed to the insecticide. Exposure to fipronil decreased the swimming speed of Belostomatidae individuals, possibly due to its neurotoxic effect. Exposure to the insecticide and the herbicide altered prey intake by predators, which could negatively influence the complex prey-predator relationship and the functioning of aquatic ecosystems in contaminated areas.

Bacterial agents for controlling anthracnose and soft rot in strawberries: present status and perspectives

ABSTRACT Anthracnose and soft rot, caused by the fungal pathogens Colletotrichum spp. and Rhizopus spp., respectively, stand as prominent diseases impacting strawberries during the postharvest phase, resulting in significant economic losses. The use of conventional treatments involving chemical fungicides has been discouraged since they negatively impact the environment and human health. In this context, alternative strategies, such as biological control, have been required for organic and sustainable agriculture. While bacterial agents, such as Bacillus spp., are successfully used in commercial formulations to control postharvest diseases, there are other bacteria exhibiting antagonistic activities that hold potential in plant disease management. Hence, the current review aims to investigate studies developed over the past decade describing the primary bacterial genera with the potential to inhibit the causal agents of soft rot and anthracnose. Additionally, it aims to provide an overview of biocontrol mechanisms for suppressing these pathogens, detailing methods of application in both pre and postharvest phases, along with strategies for enhancing the efficacy of biocontrol agents. The findings of this review demonstrate that numerous bacterial species possess the ability to effectively control anthracnose and soft rot, both in growing plants and harvested strawberries. This expands the available forms of control and minimises the occurrence of resistant pathogens. Furthermore, the application of postharvest techniques such as edible coatings and bacterial encapsulation proves advantageous, extending fruit shelf life and reducing strawberry decay.

Elucidating the effects of pure glyphosate and a commercial formulation on early life stages of zebrafish using a complete biomarker approach: All-or-nothing!

The search for new methods in the toxicology field has increased the use of early life stages of zebrafish (Danio rerio) as a versatile organism model. Here, we use early stages of zebrafish to evaluate glyphosate as pure active ingredient and within a commercial formulation in terms of oxidative stress. Biomarkers involved in the oxidative status were evaluated along with other markers of neurotoxicity, genotoxicity, cytotoxicity, energy balance and motor performance, and the selected tools were evaluated by its sensitivity in determining early-warning events. Zebrafish embryos exposed to glyphosate active ingredient and glyphosate-based formulation were under oxidative stress, but only the commercial formulation delayed the embryogenesis, affected the cholinergic neurotransmission and induced DNA damage. Both altered the motor performance of larvae at very low concentrations, becoming larvae hypoactive. The energy balance was also impaired, as embryos under oxidative stress had lower lipids reserves. Although data suggest that glyphosate-based formulation has higher toxicity than the active ingredient itself, the most sensitive biomarkers detected early-warning effects at very low concentrations of the active ingredient. Biochemical biomarkers of defense system and oxidative damage were the most sensitive tools, detecting pro-oxidant responses at very low concentrations, along with markers of motor performance that showed high sensitivity and high throughput, suitable for detecting early effects linked to neurotoxicity. Alterations on morphology during embryogenesis showed the lowest sensitivity, thus morphological alterations appeared after several alterations at biochemical levels. Tools evaluating DNA damage and cell proliferation showed mid-sensitivity, but low throughput, thus they could be used as complementary markers.

Elevated fucose content enhances the cryoprotective performance of anionic polysaccharides

Biological cryopreservation often involves using a cryoprotective agent (CPA) to mitigate lethal physical stressors cells endure during freezing and thawing, but effective CPA concentrations are cytotoxic. Hence, natural polysaccharides have been studied as biocompatible alternatives. Here, a subset of 26 natural polysaccharides of various chemical composition was probed for their potential in enhancing the metabolic post-thaw viability (PTV) of cryopreserved Vero cells. The best performing cryoprotective polysaccharides contained significant fucose amounts, resulting in average PTV 2.8-fold (up to 3.1-fold) compared to 0.8-fold and 2.2-fold for all non-cryoprotective and cryoprotective polysaccharides, respectively, outperforming the optimized commercial CryoStor™ CS5 formulation (2.6-fold). Stoichiometrically, a balance between fucose (18–35.7 mol%), uronic acids (UA) (13.5–26 mol%) and high molecular weight (MW > 1 MDa) generated optimal PTV. Principal component analysis (PCA) revealed that fucose enhances cell survival by a charge-independent, MW-scaling mechanism (PC1), drastically different from the charge-dominated ice growth disruption of UA (PC2). Its neutral nature and unique properties distinguishable from other neutral monomers suggest fucose may play a passive role in conformational adaptability of polysaccharide to ice growth inhibition, or an active role in cell membrane stabilization through binding. Ultimately, fucose-rich anionic polysaccharides may indulge in polymer-ice and polymer-cell interactions that actively disrupt ice and minimize lethal volumetric fluctuations due to a balanced hydrophobic-hydrophilic character. Our research showed the critical role neutral fucose plays in enhancing cellular cryopreservation outcomes, disputing previous assumptions of polyanionicity being the sole governing predictor of cryoprotection.

Quantifying the removal of polybrominated diphenyl ethers (PBDEs) in physical, chemical, and biological sludge treatment systems

Polybrominated diphenyl ethers (PBDE) are priority contaminants historically used as flame retardants. PBDEs are known to occur in wastewater biosolids posing potential concerns with the beneficial land application of the biosolids. This study evaluated the removal of 21 congeners in nine full-scale sludge treatment systems including pelletization (P), alkaline stabilization (AS), and aerobic (AE) and anaerobic (AN) digestion. It is the first study to conduct a mass balance analysis of a broad spectrum of PBDEs during physical, chemical, and biological sludge treatment. The PBDE congener pattern in raw sludge and biosolids samples was consistent with commercial formulations. The fully brominated congener BDE-209 dominated biosolids from all sites with an average concentration of 620 ng/g dry weight (dw), followed by BDE-99 (173 ng/g dw) and BDE-47 (162 ng/g dw). Mass balance analysis on the P and AS processes showed no change in PBDE mass flows with treatment. However, aerobic and anaerobic digestion processes reported significant levels of removal and formation of individual congeners, though the results were not consistent between facilities. One aerobic digestion process (AE2) reported an overall average removal of 48%, whereas the other (AE1) reported very high levels of accumulation of tri- and tetraBDE congeners. Similarly, there were significant variations in PBDE behavior across the five anaerobic digestion plants studied. The plant with the longest solids retention time (SRT) (AN1) reported a moderate removal (50%) of overall PBDE loading and lower congeners, whereas other plants (AN2-AN5) showed significant low (−19%) to high (−166%) levels of formation of lower congeners. The results suggest that reduced SRTs result in formation of lower congeners while extended SRTs can lead to moderate removal of some PBDEs. Conventional sludge treatment result in low to moderate PBDE removal and advanced thermal conversion technologies may be needed to improve the contaminant removal during sludge treatment.

Effects of Different Cover Crops and Amendments on Soil and Crop Properties in Organic Vegetable Production

The impacts of two different cover crop (CC) strategies, as well as compost, silicon (Si), and biocontrol (BC) soil amendments were evaluated on soil chemical and biological properties, crop development and yield, and disease and pest issues in organic vegetable production, as represented by legume (green snap bean), cucurbit (green zucchini squash), and solanaceous (sweet pepper) vegetable crops, in a three-year field trial in Maine, USA. A multi-species CC mixture (6 crops, including legumes, grasses, and brassicas) was compared with a standard winter rye CC for potential benefits on soil properties and biomass production. Soil amendments included a commercial organic fertilizer alone, composted dairy manure, compost plus BC (commercial formulations of Trichoderma and Streptomyces spp.), and compost plus Wollastonite, a natural source of Si. Poor stand establishment of some components of the multi-species CC mixture resulted in lower biomass and ground cover relative to winter rye, but had no effect on crop development or yield. Compost amendments increased soil pH, organic matter, and nutrient contents, as well as yields of bean, zucchini, and peppers relative to a fertilizer-only treatment. Additions of Si increased Si content in plant leaves and reduced powdery mildew on squash and leaf necrosis of beans. In the absence of substantial soilborne disease, BC provided only marginal reductions in powdery mildew and leaf necrosis and no effects on yield. These results help define specific management practices to improve organic vegetable production and provide useful information and options for growers.

Development of an Accelerated Rotator-based Drug Release Method for the Evaluation of Bupivacaine Multivesicular Liposomes.

A multivesicular liposome (MVL) is a liposomal vehicle designed to achieve sustained release characteristics for drugs with short half-lives. For example, a commercial MVL formulation of bupivacaine has been approved by the U.S. Food and Drug Administration for local and regional analgesia. For complex formulations like those containing MVLs, challenges in developing an in vitro release testing (IVRT) method may hinder generic development and regulatory approval. In this study, we developed an accelerated rotator-based IVRT method with the ability to discriminate bupivacaine MVLs with different quality attributes. Three IVRT experimental setups including mesh tube, horizontal shaker, and vertical rotator were screened to ensure that at least 50% of bupivacaine can release from MVLs in 24h. Sample dilution factors, incubation temperature, and the release media pH were optimized for the IVRT. The reproducibility of the developed IVRT method was validated with commercial bupivacaine MVLs. The discriminative capacity was assessed via comparing commercial and compromised bupivacaine MVL formulations. The rotator-based release setup was chosen due to the capability to obtain 70% of drug release within 24h. The optimized testing conditions were chosen with a 50-fold dilution factor, a temperature of 37ºC, and a media pH of 7.4. An accelerated rotator-based IVRT method for bupivacaine MVLs was developed in this study, with the discriminatory ability to distinguish between formulations of different qualities. The developed IVRT method was a robust tool for generic development of MVL based formulations.

Liver and kidney damage in quail embryos hatched from eggs sprayed once with chlorpyrifos during artificial incubation.

Birds have enough conditions to be used as bioindicators for the presence of environmental contaminants. Notably, chlorpyrifos (CPF) remains extensively employed in Argentina, particularly in fruit plantations and livestock settings This study aimed to assess the potential impacts of CPF on common quail (Coturnix coturnix) embryos through external eggshell application during key embryonic stages (days 1, 4, and 14). Concentrations equivalent to those used in fruit applications, 5× and 10 × (38.4, 192, and 384µg/egg), were employed. A 48% commercial formulation of CPF (Pirfos - Icona®) was utilized. An increase in embryonic deaths, as well as a statistical association between the degree of liver or kidney lesions and CPF concentrations was observed. The results suggest that CPF can induce embryotoxic effects with a single exposure to quail embryos and compromise the viability of the organisms. The study suggests a potential exposure risk for embryos through contact with the insecticide on the eggshell's exterior.

An Integrated Computational and Experimental Approach to Formulate Tamanu Oil Bigels as Anti-Scarring Agent.

Tamanu oil has traditionally been used to treat various skin problems. The oil has wound-healing and skin-regenerating capabilities and encourages the growth of new skin cells, all of which are helpful for fading scars and hyperpigmentation, as well as promoting an all-around glow. The strong nutty odor and high viscosity are the major disadvantages associated with its application. The aim of this study was to create bigels using tamanu oil for its anti-scarring properties and predict the possible mechanism of action through the help of molecular docking studies. In silico studies were performed to analyze the binding affinity of the protein with the drug, and the anti-scarring activity was established using a full-thickness excision wound model. In silico studies revealed that the components inophyllum C, 4-norlanosta-17(20),24-diene-11,16-diol-21-oic acid, 3-oxo-16,21-lactone, calanolide A, and calophyllolide had docking scores of -11.3 kcal/mol, -11.1 kcal/mol, -9.8 kcal/mol, and -8.6 kcal/mol, respectively, with the cytokine TGF-β1 receptor. Bigels were prepared with tamanu oil ranging from 5 to 20% along with micronized xanthan gum and evaluated for their pH, viscosity, and spreadability. An acute dermal irritation study in rabbits showed no irritation, erythema, eschar, or edema. In vivo excisional wound-healing studies performed on Wistar rats and subsequent histopathological studies showed that bigels had better healing properties when compared to the commercial formulation (MurivennaTM oil). This study substantiates the wound-healing and scar reduction potential of tamanu oil bigels.

Bioinspired Herbicides-Ionic Liquids or Liquid Cocrystals?

This research provides information about combinations of several amino acids, including l-proline (Pro), l-arginine (Arg), and l-histidine (His), with phenoxyacetic acid herbicides (MCPA and 2,4-D). Five amino acid ionic liquids (AAILs), one amino acid higher-melting salt (AAHMS), and two amino acid liquid cocrystals (AALCs) were obtained in high yields (>90%). The ionization of the six new structures was confirmed by NMR, IR, and molecular modeling. X-ray crystallography was used to definitively confirm the binding location of the mobile hydrogen. Furthermore, we propose a computational method for estimating the energy of specific hydrogen bond(s) in AAIL crystals based on the NBO and QTAIM hydrogen bond parameters obtained by model calculations. An in-depth analysis of the structures allowed to answer the question posed in the title, ionic liquids or liquid cocrystals? AAILs based on arginine and histidine were obtained. In contrast, combining proline with MCPA and 2,4-D led to AALCs. Finally, the compounds were analyzed to measure their herbicidal activity. These studies proved that the novel form of MCPA or 2,4-D improved its ability to control weeds compared to commercial formulations containing the same active ingredients.

Biostimulants Improve Plant Performance of Rosemary Growth in Agricultural Organic System

Rosemary (Rosmarinus officinalis L.) is an evergreen and a xerophytic shrub that is frequently employed in culinary, decorative, and industrial applications. It shows significant variations in biomass and essential oil (EO) yield due to effects of biotic and abiotic factors. Biostimulants are widely used in agriculture, and in organic agricultural systems, they may contribute significantly to the stability and/or to the increase in crop yields, ensuring respect for the environment. The aim of this study was to assess the effect of different types of biostimulants on the yield performance of rosemary. Four commercial formulations of biostimulants based on Eklonia maxima, Ascophyllum nodosum, fulvic acids, and protein hydrolysates were used for the tests. Water was used as a control. Six applications were performed. At harvest, several morphological and productive parameters were determined. All parameters were affected by biostimulant application. The highest fresh and dry yields were obtained with the application of fulvic acids (13.1 t ha−1 of fresh biomass and 4.3 t ha−1 of dry biomass) and protein hydrolysates (13.6 t ha−1 of fresh biomass and 4.4 t ha−1 of dry biomass) with values 60% higher than that of the control. Regarding the EO content in the dry biomass, the highest value (1.72% v/w) was found in control plants, whilst the lowest (1.14%) was observed in plants treated with fulvic acids. In the case of the EO yield, the lowest value (46.5 kg ha−1) per unit area was found in the control. The application of Eklonia maxima and protein hydrolysates produced the highest EO yield values, 65 kg ha−1 and 66.5 kg ha−1, respectively. This study highlights the use of biostimulants to increase the yield performance of rosemary when grown in an organic agricultural system.

Two protocols using fluralaner for Rhipicephalus microplus strategic control on taurine cattle in a tropical region

BackgroundThe present study aimed to evaluate the effects of different treatment strategies using a new commercial formulation containing pour-on fluralaner on Rhipicephalus microplus infestation in cattle and in pastures in a tropical climate region where up to five generations of this tick species can occur per year.MethodsForty-five naturally infested cattle were divided into three experimental groups: T01, treated with fluralaner (2.5 mg/kg) pour-on every 42 days; T02, the cattle received the first treatment with fluralaner on Day 0 but the next treatment involved a weekly visual evaluation; T03, control, received palliative treatment with a spray formulation when the group mean was ≥ 30 ticks. Counts of female R. microplus were performed weekly until day 343, and larval counts on pasture were performed on Days 0, 30, and 60 and every 30 days until Day 330.ResultsUsing fluralaner, six applications were performed in Group T01, and four were performed in Group T02. In the control group (T03), it was necessary to perform eight palliative acaricide treatments with the spray formulation. The animals in T01 and T02 showed lower mean tick counts (p ≤ 0.05) than the control group (T03) on 28 and 27 of the 49 evaluated dates, respectively. In the paddock where the animals were kept as controls, the R. microplus larvae counts increased to 1458. In the paddocks where the animals were treated with fluralaner, the number was ≤ 19 per paddock during the study.ConclusionsThe different strategic treatment protocols performed with pour-on fluralaner (2.5 mg/kg) over a year in taurine cattle in a tropical region with a history of up to five annual generations of cattle ticks were effective, maintaining levels of R. microplus infestations in animals and in pastures close to zero in most of the study. Depending on the retreatment criterion adopted, the number of applications per year may be lower, resulting in a reduction in the mean cost of acaricide treatment per year and lower exposure of R. microplus populations to the active ingredient, resulting in lower resistance and selection pressure.Graphical

Toxicity assessment of commercial herbicide formulations to Eisenia andrei (Bouché, 1972) in oxisols

The use of long-residual herbicides can have adverse effects on terrestrial ecosystems. This study assessed the acute and chronic toxicity and avoidance behavior of Eisenia andrei earthworms exposed to nominal concentrations of clomazone, indaziflam, and sulfentrazone, using recommended commercial formulations for sugarcane cultivation. The formulations included Gamit® 360 CS (360 g L−1 of the active ingredient - a.i. - clomazone), Boral® 500 SC (500 g L−1 of a.i. sulfentrazone), and Alion® (500 g L−1 of a.i. indaziflam). Boral® 500 SC induced avoidance at concentrations as low as 1 mg kg−1, while Gamit® 360 CS and Alion® exhibited higher avoidance at 50–75 mg kg−1 and 75–100 mg a.i. kg−1, respectively. Reproduction tests showed significant impacts from Gamit® 360 CS (EC50: 0.572 mg kg−1, EC10: 0.2144 mg kg−1) and Boral® 500 SC (EC50: 0.3941 mg kg−1, EC10: 0.134 mg kg−1). Acute toxicity tests indicated moderate toxicity for Gamit® 360 CS (LC50: 184.12 mg kg−1) and Boral® 500 SC (LC50: 1000 mg kg−1). Gamit® 360 CS reduced biomass at all concentrations, while Boral® 500 SC influenced only at higher levels (500 and 1000 mg kg−1). Results suggest significant acute risks with Gamit® 360 CS, while chronic exposure raises concerns for both Gamit® 360 CS and Boral® 500 SC, indicating potential long-term risks. Alion®'s acute effects were inconclusive, but chronic exposure hints at a possible risk. These findings provide crucial insights for environmental agencies establishing protective limits against herbicide exposure to non-target soil invertebrates.

2,4-D Herbicide-Induced Hepatotoxicity: Unveiling Disrupted Liver Functions and Associated Biomarkers.

2,4-dichlorophenoxyacetic acid (2,4-D) is a widely used herbicide worldwide and is frequently found in water samples. This knowledge has prompted studies on its effects on non-target organisms, revealing significant alterations to liver structure and function. In this review, we evaluated the literature on the hepatotoxicity of 2,4-D, focusing on morphological damages, toxicity biomarkers and affected liver functions. Searches were conducted on PubMed, Web of Science and Scopus and 83 articles were selected after curation. Among these studies, 72% used in vivo models and 30% used in vitro models. Additionally, 48% used the active ingredient, and 35% used commercial formulations in exposure experiments. The most affected biomarkers were related to a decrease in antioxidant capacity through alterations in the activities of catalase, superoxide dismutase and the levels of malondialdehyde. Changes in energy metabolism, lipids, liver function, and xenobiotic metabolism were also identified. Furthermore, studies about the effects of 2,4-D in mixtures with other pesticides were found, as well as hepatoprotection trials. The reviewed data indicate the essential role of reduction in antioxidant capacity and oxidative stress in 2,4-D-induced hepatotoxicity. However, the mechanism of action of the herbicide is still not fully understood and further research in this area is necessary.

Development of a Palm Kernel-Based Emulgel Loaded with Mitracarpus scarber Leaf Extracts for Effective Anti-Inflammatory Activity

Mitracarpus scaber is a tropical herb used in ethnomedicine for the treatment of inflammation and a variety of other diseases. The aim of this study was to develop a palm kernel-based emulgel containing Mitracarpus scarber and examine the product's physicochemical characteristics and anti-inflammatory efficacy on Wister rats. Different batches of emulgel comprising Mitracarpus scarber leaf extracts (MSE), different proportions of oils (palm kernel oil and/or liquid paraffin), and surfactants (tween-80 and/or Cremophor EL-30) were prepared. The freshly prepared and 5-month-old formulations, stored at room temperature (28 ± 2 °C), were evaluated for rheology, syneresis, and extract/excipient compatibility using Fourier transform infrared (FT-IR) spectroscopy. The spreadability, extrudability, and anti-inflammatory activity of the optimal formulation were compared with those of a commercial anti-inflammatory emulgel. Even after five months of storage, the created emulgels remained olive green and creamy, with a homogeneous texture, consistency, and glossy appearance. The optimized batch containing palm kernel oil, tween-80, and xanthan gum had the most stable characteristics, with no significant change in pH (P = 1.000) or FT-IR spectrum after 5 months of storage. It exhibited spreadability and extrudability of 12.75 cm2 and 600 g/cm2, respectively, compared to 16.82 cm2 and 400 g/cm2 for the commercial formulation. Four hours post-treatment, the anti-inflammatory effect of the optimized batch was significantly (P < 0.05) higher than that of the aqueous MSE dispersion. The obtained results demonstrate the prospects of palm kernel-based emulgel loaded with Mitracarpus scarber leaf extracts as an innovative therapeutic approach to inflammation treatment. Keywords: Inflammation, Emulgel, Mitracarpus scarber, Palm kernel oil, Formulation

Resposta anamnéstica contra o vírus da diarreia viral bovina e herpesvírus bovino tipo 1 em novilhas Holandesas jovens vacinadas com quatro diferentes formulações comerciais

ABSTRACT: This study evaluated the vaccine-induced serological response after administering four commercial formulations containing Bovine Viral Diarrhea Virus (BVDV) type-1, BVDV-2, and Bovine Herpesvirus type 1 (BoHV-1) to young heifers with circulating maternal antibody titers. The study also determined the anamnestic response to vaccinations after the complete metabolization of maternal antibodies when the calves reached six months. Eighty-seven Holstein heifers were selected and randomly distributed into four experimental groups based on the vaccine administered. The four vaccine-based experimental groups were as follows: vaccine A (n = 22), vaccine B (n = 22), vaccine C (n = 24), and vaccine D (n = 19), given on the 60th, 90th, and 180th day of life, respectively. Virus-neutralization (VN) tests were performed at baseline (day 60) and 30 days after administering the second (day 120) and third doses (day 210). We analyzed the effect of vaccine group (P ≤ 0.001), day of vaccination (P ≤ 0.001), and group versus vaccine day interaction (P ≤ 0.001) for antibody titers produced against BVDV-1, BVDV-2, and BoHV-1 using the PROC MIXED method (Statistical Analysis System -SAS 9.4). Antibody titers against BVDV-1, BVDV-2, and BoHV-1 were similar at baseline and on day 60 of life. The mean antibody titers were constant and persisted against BVDV-1 in heifers immunized with vaccines A and C. Heifers immunized with vaccine A alone had a similar effect against BVDV-2. Regarding BoHV-1, the antibody titers decreased between days 60 and 210 in groups B, C, and D. The antibody titer for heifers in group A also decreased between days 60 and day 120, and an intense increase in titers was observed on day 210. After being immunized with formulations B, C, and D, the frequency of animals with titers above protective levels for BVDV-1, BVDV-2, and BoHV-1 was very low or null. Indicators of anamnestic response were observed in heifers vaccinated with formulation A only. Therefore, it can be concluded that passive immunity negatively interferes with antibody production after vaccination.

Simultaneous Spectrophotometric Determination of Piroxicam, Naproxen, Diclofenac Sodium and Mefenamic Acid in Pharmaceutical Formulations by Partial Least Squares Method

A chemometric method, partial least squares regression (PLS) was applied for the simultaneous determination of piroxicam (PIR), naproxen (NAP), diclofenac sodium (DIC), and mefenamic acid (MEF) in synthetic mixtures and commercial formulations. The proposed method is based on the use of spectrophotometric data coupled with PLS multivariate calibration. The Spectra of drugs were recorded at concentrations in the linear range of 1.0 - 10 μg mL-1 for NAP and from 1.0 - 20 μg mL-1 for PIR, DIC, and MEF. 34 sets of mixtures were used for calibration and 10 sets of mixtures were used for validation in the wavelength range of 200 to 400 nm with the wavelength interval λ = 1 nm in methanol. This method has been used successfully to quantify drugs in pharmaceutical formulations with no interference from excipients. The proposed method is simple, quick, and can be used as an alternative analysis tool in drug and formulation quality control as well as process control.

A dual-function chromogenic and fluorogenic benzofurazan probe for plazomicin and its innovative utility for development of two microwell assays with high throughput for analysis of drug substance and pharmaceutical formulations.

Plazomicin (PLZ) is a novel aminoglycoside which has been recently approved by The US Food and Drug Administration for the treatment of complicated urinary tract infections including acute pyelonephritis, caused by certain Enterobacteriaceae, in adult patients with limited or no options for alternative treatment. This study focuses on the development of microwell-based photometric and fluorometric assays for the quantitative determination of PLZ in its bulk drug substance and commercial pharmaceutical formulations (Zemedri® injections). Both assays utilize the dual-function chromogenic and fluorogenic properties of the 4-fluoro-7-nitrobenzofurazan (NBD-F) probe. The reaction between PLZ and NBD-F, conducted in a borate buffer at pH 8, resulted in the formation of a colored and fluorescent reaction product. The product exhibited maximum light absorption at 473 nm and emitted fluorescence at 541 nm when excited at 473 nm. Factors influencing the reaction between PLZ and NBD-F were thoroughly investigated, and optimal conditions were determined. Under the optimized reaction conditions, calibration curves were generated to establish the relationship between absorbance and fluorescence intensities of the reaction product with the corresponding PLZ concentrations. The absorbance-concentration relation was linear in a PLZ concentration range of 20-800 μg mL-1 with a limit of quantitation of 25 μg mL-1, while the fluorescence-concentration relation was linear in the concentration range of 0.05-1.5 μg mL-1 with a limit of quantitation of 0.08 μg mL-1. Both assays underwent validation and were successfully applied to the quantitation of PLZ in its bulk drug substance and pharmaceutical formulations (injections) with satisfactory accuracy and precision. The eco-friendliness/greenness assessment of the assays demonstrated that both assays comply with the requirements of green analytical chemistry approaches. Furthermore, the proposed microwell assay plates allowed for the simultaneous handling of numerous samples with micro-volumes, enabling high-throughput analysis. In conclusion, this study represents the first evaluation of NBD-F as a dual-function probe for the microwell-based photometric and fluorometric determination of PLZ. The developed assays serve as valuable analytical tools for the quality control of PLZ's bulk drug substance and pharmaceutical formulations.