Commercial Farms Research Articles

Non-Conventional Data for Farming-Related Air Pollution: Contributions to Modelling and Risk Assessment in the Lombardy Region, Italy

Air pollution is one of the most critical global health concerns today. While emissions from industrial activities and combustion processes are the primary threats to air quality, intensive farming activities also contribute significantly, especially through ammonia emissions that promote the formation of secondary pollutants, such as particulate matter. Advancements in air quality research have been achieved by enhancements in emissions characterisation, modelling techniques, and sensor technology, expanding the availability of air pollution data beyond traditional ground sensor observations, which are often lacking in rural agricultural areas. Accordingly, this paper demonstrates the advantages of integrating traditional and non-conventional data to investigate farming-related air pollution through a case study in the Lombardy Region, Northern Italy. The study incorporates an array of data sources, including ground sensors and atmospheric composition model estimates. The concurrent utilisation of these diverse datasets is explored through machine learning modelling, focusing on assessing the influence of agricultural activities on particulate matter distribution patterns. Finally, the reliability of non-conventional air pollution data for health risk assessment applications is also investigated. The paper critically discusses the main findings based on empirical results, highlighting the significance of integrating multiple data sources to complement traditional air quality monitoring while outlining the main limitations in terms of the accuracy and usability of such non-conventional data.

Study Report on Optimum Utilisation Plan of Ngoma 22 Irrigation Scheme

The study was conducted in Ngoma 22 irrigation scheme located in Remera and Rurenge sectors of Ngoma District in Eastern Province of Rwanda between November 2018 and January 2019. It was recommended by RAB Board of Directors. The methodology used encompasses the study area, sampling procedures, data collection and analysis tools. The main data collection tool used was FGD which targeted site agronomists and household head farmers producing rice, fruits and vegetables as high value crops in the scheme area. Through pair wise ranking technique most profitable cash crops in the scheme were ranked as follows: (1) tree tomato, (2) maracuja, (3) egg plants, (4) water melon, (5) tomato, (6) irish potatoes, (7) french beans, (8) onions, (9) carrots, (10) cabbages, (11) beetroots. From this point of views, findings from the study show only two most profitable crops: maracuja with 696 Frw/kg followed by tree tomato with Frw 249 Frw/kg. The third ranked cash crop being Irish potato (with a loss of -40 Frw/kg), the fourth is egg plant with a loss of- 177 Frw. The existing paddy rice production has shown a loss of – 270 Frw inspite the water availability all the year-round. Maize and bush bean show respectively losses of -543 Frw/kg and -1743 Frw. Hence it is understandable why farmers in the scheme area have already abandoned maize and beans cultivation. Findings show also water fees (20,000 Frw/season) determined based on maintenance costs and farmers income. For capacity building model, more than one thousand beneficiary farmers in Ngoma 22 Irrigation Scheme need strong farmer organisation for ownership of the scheme and increased profitability. To that effect, they need continued capacity building as stated in the tripartite IMTA: administrative and financial management, record keeping, and conflicts resolution management, integrated water management, operation and maintenance of irrigation infrastructures, best agricultural practices for sustainable farmers’ organisations and commercial farming. Key recommendations include fixing minimum prices for agricultural products in relation to costs of production- or setting subsidy prices, increase yield for each crop by meeting all requirements: lime, fertilisers and improvement of irrigation technologies in order to reach automatically increased yield. Farmers should continue to leave out traditional crops and adopt profitable cash crops identified in this study (e.g maracuja and tree tomato). They should also increase the ownership spirit through IWUA and cooperatives performance. They should adopt collective marketing of their products and pay water fees per season.

MeHA: A Computational Framework in Revealing the Genetic Basis of Animal Mental Health Traits Under an Intensive Farming System-A Case Study in Pigs.

Intensively farmed animals such as pigs inevitably experience a certain degree of psychological stress, which leads to a reduction in production performance. Mental health traits are currently difficult to measure, resulting in a gap in understanding their genetic basis. To address this challenge, we propose a computational framework called mental health of animals (MeHA), capable of revealing genes related to animal mental health traits. Using MeHA, we identified 109 candidate genes associated with pig mental health and discovered their intricate connections with critical functions, such as memory, cognition, and neural development, which are essential components of mental health and cognitive performance. Importantly, our findings provide evidence of the potential impact of these genes on economically important traits, including meat quality and piglet survival. This research underscores the importance of genetic studies in enhancing our understanding of animal behavior and cognition, as well as promoting agricultural practices. By applying our approach to study the genetic basis of mental health in pigs as a case, we confirmed that our framework is an effective way to reveal genetic factors affecting animal mental health traits, which contributes to animal welfare and has potential implications for understanding human mental disorders.

Markov Chain Analysis of Land-use Changes in India and Maharashtra

Dynamics of Agriculture resembles the history of agriculture in India which examines the emphasizing significance to the nation's legacy, culture and civilization. India's agriculture changed after independence from a food-scarce nation to one that exports food this comes true primarily due to the result of scientific advancements. The present study is based on secondary data with the use of Markov Chain analysis to ascertain land-use changes in India and Maharashtra state. Changes in land use brought on by agriculture, urbanization and environmental factors significantly impact resource management and sustainability. We build a Markov Chain model with secondary land utilization data from government reports to measure the transition probabilities between various land use categories over time. The Markov chain model is easy to understand and communicate. It effectively captures transitions between land-use changes over time. The model works with limited data as compared to other complex models, making it accessible for studies. The results of the study emphasize dynamic interactions between various land-use patterns in India and Maharashtra. There we have concluded a stable land-use changing pattern. A trend towards intensive cropping has been observed during this study. This study reveals important trends in land conversion, most notably the growth of urban areas at the expense of forested and agricultural areas. The predictive abilities of model highlight the significance of well-informed policy-making for sustainable land management by offering insights into potential future land use scenarios.

Exploring the association between SLC11A1 and CARD15 gene polymorphisms and tuberculosis susceptibility in Holstein cattle

Abstract. Bovine tuberculosis (bTB) is a contagious disease that has a socio-economic impact on familial and industrial farms. Genetic selection can improve resistance against bTB. This study aimed to characterize the links between SLC11A1 and CARD15 gene polymorphisms and tuberculosis (TB) susceptibility. In total 200 animals were used (50 cases and 150 controls). Polymorphisms in the SLC11A1 and CARD15 genes were identified and analyzed with the restriction fragment length polymorphism (PCR-RFLP) method. Our results report the PstI PCR-RFLP marker for the SLC11A1-SNP1 site and the StyI PCR-RFLP marker for the CARD51-SNP1 site in Tunisian Holstein cows. Statistical analysis showed a significant association between SLC11A1-SNP1, CARD15-SNP1 and susceptibility/resistance to TB (P < 0.05). Two SLC11A1-SNP1 genotypes were susceptible to tuberculosis, i.e., the heterozygote CG and the homozygote CC, while one SLC11A1-SNP1 genotype, i.e., the GG mutated homozygote, was less susceptible to infection. Concerning the CARD15 gene, two genotypes are highly sensitive to the incidence of bovine tuberculosis, i.e., AA and AG, while the GG genotype is more beneficial and more tolerant of Mycobacterium bovis. SLC11A1 and CARD15 are two useful candidate genes associated with tuberculosis, and this information can be used to improve the health status of domestic cattle and humans.

Dietary glycerides of valerate ameliorate diarrhea and impact intestinal physiology and serum biomarkers in weaned piglets infected with enterotoxigenic Escherichia coli F18.

In the commercial swine farm setting, the post-weaning period is a critical window during which piglets are highly susceptible to infection and enterotoxigenic E. coli (ETEC)-associated diarrhea. Short chain fatty acids and their glycerides are compounds which may influence intestinal health; however, valerate is one that has not been well-characterized for its role as a dietary supplement. Therefore, the major objective of this experiment was to investigate two forms of valerate glycerides on diarrhea, intestinal physiology, and systemic immunity of weaned pigs experimentally infected with ETEC F18. Dietary treatments included a control diet and three additional diets supplemented with 0.075% monovalerin, 0.1% monovalerin, or 0.1% trivalerin, respectively. Piglets were weaned (21-24 d of age), individually housed, and experimental diets were fed throught the 28-day trial period. After a seven-day period, all piglets were inoculated on three consecutive days with 1010 CFU ETEC F18/3mL. Growth performance was monitored throughout the trial and daily diarrhea scores were recorded. Rectal swabs were collected for bacterial culture to confirm the presence or absence of β-hemolytic coliforms throughout the trial. Serum samples were collected and analyzed for inflammatory biomarkers on d 0, 3, 6, and 21 post-inoculation (PI) and untargeted metabolomics on d 6 PI. Intestinal mucosa and tissue sections were harvested from pigs sacrificed on d 7 PI for gene expression and histology analysis. All data, except for frequency of diarrhea and metabolomics, were analyzed by ANOVA using the PROC MIXED of SAS. Dietary trivalerin reduced (P < 0.05) the frequency of severe diarrhea over the entire trial period and the frequency of β-hemolytic coliforms on d 7 PI compared with control. The intestinal villus height on d 7 PI in jejunum tissue was increased (P < 0.05) in pigs fed trivalerin. The mRNA expression of TNF-α was decreased (P < 0.05) in the trivalerin group, while that of ZO1 was increased (P < 0.05) compared with control. Throughout the trial, serum TNF-α was reduced in pigs fed trivalerin compared with control. Serum metabolites, adenosine, inosine, and shikimic acid were reduced (P < 0.05) on d 6 PI in all treatment groups compared with control. In conclusion, the present results indicate supplementing dietary valerate glycerides exhibited beneficial impacts on diarrhea, inflammation, and intestinal gene expression of piglets during the post-weaning period.

Opportunities and Challenges on Natural Farming

Natural Farming, an ecological and sustainable agricultural practice, emphasizes minimal human intervention and the use of natural resources for crop cultivation. Unlike conventional farming, which relies heavily on chemical inputs, Natural Farming integrates practices such as minimal tillage and organic inputs like cow dung and plant-based preparations. This case study explores Natural Farming practices among farmers in Tamil Nadu, focusing on their experiences, opportunities, and challenges. The study includes detailed case studies of three farmers in Coimbatore District and each farmer employing different natural farming techniques and facing unique obstacles. The findings reveal that Natural Farming offers significant benefits, including reduced farming costs, improved soil health, and enhanced market opportunities for organic produce. Farmers reported that these practices lead to better crop resilience and higher-quality produce, often fetching premium prices. However, challenges persist, including difficulties in weed control, pest and disease management, labor shortages, and delay in certification, which can affect market access and profitability. The study underscores the potential of Natural Farming to transform agricultural practices by promoting sustainability and reducing dependence on chemical inputs. It also highlights the need for supportive interventions, such as enhanced market access, comprehensive farmer training, and government support to address the identified challenges and maximize the benefits of Natural Farming. This study contributes to a deeper understanding of Natural Farming's role in sustainable agriculture and its implications for farmers in Tamil Nadu.

Enhancing Water Use Efficiency and Carbon Profitability Through the Long-Term Impact of Sustainable Farming Systems

This study aims to enhance water use efficiency, maximize productivity, and minimize environmental impact through the implementation of sustainable agricultural systems using drip irrigation systems. It investigates the effects of biodynamic farming compared to those of organic and conventional methods over a six-year period and focuses on soil properties, water use efficiency, crop yield, and environmental and economic perspectives. Using a biodynamic farming system resulted in an average increase in water use efficiency of 1.96 and 10.67% for maize and 3.62 and 10.68% for faba bean and an increase in maize yield of 1.68 and 0.99%, while the faba bean yield reached 3.25 and 1.57% compared to the organic and conventional farming systems, respectively. The biodynamic system sequestered the highest average soil carbon of 6.16 tons/ha (which is equivalent to 22.45 tons/ha of CO2 emissions), representing a 13% increase compared to the organic system. Additionally, the biodynamic system yielded an increase in total net profit of 5.70 and 21.66% for the maize crop and 6.72 and 22.19% for the faba bean crop compared to the organic and conventional farming systems, respectively. The farming system significantly influenced the soil carbon sequestration and organic carbon.

Establishing perennial strips in a cultivated floodplain for habitat restoration: monitoring over 4 years and success drivers

Floodplains, one of the most biologically diverse and productive ecosystems, are under threat from intensive crop production. Implementing perennial strips alongside agricultural ditches and streams could reduce negative impacts of intensive agriculture and restore wildlife habitats in cultivated floodplains. To successfully set up perennial strips, it is important to understand the parameters that drive their establishment. Here we assessed the establishment success of reed canarygrass (RCG; Phalaris arundinacea) strips in the lake Saint Pierre (LSP) floodplain, Québec, Canada by monitoring RCG biomass and vegetation height over 4 years and identify the factors driving its establishment. A total of 26 RCG strips across six municipalities of LSP were monitored. Biomass and vegetation height of RCG increased over time to reach an average of 5048 kg/ha in year 4 and 104 cm in year 3 in established strips. The RCG established successfully in 62% of surveyed plots and three environmental parameters explained 61% of this success. Establishment of RCG was most successful when a first rain came right after seeding (&lt;3 days). High clay content and low elevation were associated with establishment failures. Overall, our results highlight the ability of RCG strips to restore dense perennial vegetation cover in cultivated floodplain, thereby providing suitable habitat for fish spawning during spring floods. This study provides significant insight into the drivers of establishment of perennial grass strips in highly constrained cultivated areas such as floodplains.

The delineation of management zones using soil quality indices for the cultivation of irrigated rice (Oryza sativa L.) in Huila, Colombia

Conventional intensive farming systems can result in degraded soil. It is therefore important to monitor this effect periodically by delineating management zones (MZ) based on soil quality indices (SQI) in order to maintain and improve the soil characteristics in a precision farming environment and obtain homogeneous rice yields. The aim of this study was to determine and spatialise SQI and delineate MZ for cultivating flooded rice in an area of Fluvisols in Huila, Colombia. Forty-one georeferenced soil samples were collected from the 0 to 20 cm layer, and the physical, chemical and biological attributes of the soil were analysed to calculate the Integrated Quality Index (IQI) and the Nemoro Quality Index (NQI) using linear scoring functions. Geostatistical tools were then used to fit semivariogram models of the SQI, and interpolated using ordinary kriging to map the MZ using the QGIS software. The IQI and NQI showed a moderate spatial correlation, which allowed three distinct MZ to be identified and delineated. Attributes, such as bulk density (Bd), total porosity (TP), soil respiration (SR), available water (AW) and soil organic matter (SOM) were significant and can be used as a guide by farmers for restoring the quality of the soil in rice production. The method proved to be effective, and provided an information base to be used in the local management of areas of rice cultivation in the study region.

The Effect of LED Lighting Durations on Growth, Yield and Quality of Mustard (Brassica juncea L.) under Greenhouse Condition

Light-emitting diodes (LEDs) show promise as a radiation source for intensive crop cultivation systems, especially for vegetables in the greenhouse. Regarding mustard greens, most studies have investigated the effects of LED lights at the seedling stage under chamber conditions for microgreens not addressed throughout their growing period up to harvest. Therefore, this study is focused to evaluate the effect of additional LED lighting time from 6 pm on the growth, yield and quality of mustard greens under a greenhouse in Kien Giang province as a typical tropical region. The experiment was arranged in a completely randomized design with 3 replications, from August 1st to September 18th, 2023. Four treatments: AL0, AL2, AL4 and AL6 correspond to lighting durations of 0, 2, 4 and 6 h of LED light, which includes a combination of 3 white lights: 1 pink light, 58 μmol·m−2·s−1 PPFD, and a Blue (18 %): Green (41 %): Red (41 %) ratio. Results showed that AL4 had the most significant positive impact on mustard plant growth, with higher dry and fresh weight, plant height, leaf width and length. However, AL6 inhibited most parameters except leaf number. Additionally, under AL4 conditions, mustard allocated more biomass to leaves, while those under AL6 conditions allocated more biomass to stems. In terms of quality, LED lighting duration negatively influenced protein content. For instance, AL0 showed the significantly greatest protein value (2.87 ± 0.08 g·kg−1) compared to others. Apart from AL0, AL6 had a considerably higher value of protein content (2.58 ± 0.2 g·kg−1) compared to AL2 (1.74 ± 0.04 g·kg−1) and AL4 (2.17 ± 0.07 g·kg−1). The additional durations of LED lighting influenced mature mustard greens’ morphology, physiology and quality, providing valuable scientific information for improving vegetable quality and quantity in tropical zones. HIGHLIGHTS 4 hours of additional LED light had the most significant positive impact on mustard plant growth, with higher dry and fresh weight, plant height, leaf width, and length. Additionally, leaf number increased under 6 hours of additional LED light. Under 4 hours of additional LED light, mustard allocated more biomass to leaves, while those under 6 hours of additional LED light allocated more biomass to stems. Lighting with LED resulted in a lower protein value, and different durations of LED lighting resulted in varying protein content values. GRAPHICAL ABSTRACT

Performance of different wheat varieties and their associated microbiome under contrasting tillage and fertilization intensities: Insights from a Swiss long-term field experiment

Winter wheat is an important global cereal crop. However, conventional farming practices, characterised by intensive tillage and high fertilizer inputs, pose significant threats to the environment. In response, more conservative management practices are being applied aiming to maintain wheat production while promoting a beneficial microbiome. Here, we evaluated the suitability of three different wheat varieties for less intensive agricultural systems, focusing on reduced tillage and fertilizer intensity. The study was conducted over two consecutive years in a Swiss long-term field experiment comparing conventional versus reduced tillage and full fertilization versus half fertilization. In addition, we investigated the composition of plant-associated microbial communities using amplicon sequencing of phylogenetic marker genes, specifically targeting bacteria and fungi in rhizosphere samples and fungi in root samples. Our results revealed that in our study wheat variety most strongly predicted grain yield and quality, independent of tillage and fertilization intensity. Specifically, wheat varieties demonstrated higher yields and N uptake in plots subjected to conventional ploughing and full fertilization compared to those under reduced tillage and half fertilization. We found no significant effect of wheat variety on the composition of microbial communities. However, tillage emerged as the primary factor influencing microbial community composition in the rhizosphere, while fertilization intensity significantly impacted fungal communities in the root system. These findings underscore the complex interplay between agronomic practices, plant genetics, and microbial dynamics in agroecosystems, emphasizing the need for holistic and adaptive approaches and their further development to ensure sustainable crop production.

Insect Production: A Circular Economy Strategy in Iceland

In this review, the multifaceted issue of food security is addressed, emphasizing the need for innovative and culturally appropriate solutions. Exploring insect livestock farming emerges as a potential remedy, offering a pathway to alleviate food insecurity and promote food sovereignty, particularly when integrated with social acceptability. Stakeholder engagement on both production and consumption fronts, coupled with sustained support, is vital for successful implementation. The expanding landscape of commercial insect farming in the West prompts questions about its broader scalability and equitable deployment, especially for vulnerable populations. Existing research gaps underscore the need for a coordinated effort across international, national, and legal frameworks to effectively integrate insect farming into existing agricultural systems. In this review, we have delved into the industrial-scale production processes of mealworms and black soldier flies (BSFs), known for their high protein content and organic waste conversion, covering small and industrial cultivation methods, offering insights into mealworm production life cycles, innovative rearing systems, and harvesting techniques. This review concludes with climate-specific recommendations for insect facilities, stressing the importance of sustainable practices, continuous research and development, effective market strategies and economic feasibilities in Iceland. In the context of escalating demand for sustainable protein sources, industrial-scale insect production emerges as a pivotal player in addressing global food security challenges.

Layer segmented filamentous bacteria colonize and impact gut health of broiler chickens.

In commercial poultry farms, chicks hatch away from their progenitors from which they acquire key host-specific microbiota, like segmented filamentous bacteria (SFB) involved in gut maturation in early life. This study investigated whether providing chicken SFB to newly hatched broilers would increase their gut maturation and resistance to bacteria relevant to broiler and human health. One-day-old Ross308 broilers were orally treated with either phosphate-buffered saline (CON) or layer-derived SFB (D-SFB). On days 5, 10, 17, and 24, feces were collected to detect and enumerate SFB and Enterobacteriaceae. On days 8, 15, 22, and 29, birds were euthanized, intestinal samples were collected to detect and enumerate SFB through quantitative PCR (qPCR) and microscopy and expression of genes associated with gut immune function through reverse transcription-qPCR. This study showed that, despite their host specificity, layer SFB can colonize their genetically distinct relative broilers. Ileal SFB colonization was accelerated by a week with the SFB treatment and covered the proximal, medial, and distal sections of the ileum. Colonization of the ileum by SFB in early life highly activated gene expression of intestinal barrier proteins and cytokines, e.g., IL-10 and IFNγ but not IL-17. SFB treatment reduced the level of Enterobacteriaceae in the gut and provided superior resistance to intestinal and extraintestinal pathogens as tested in vitro. Overall, early gut colonization of SFB is imperative for the maturation of the gut immune system and the establishment of a homeostatic gut environment. Improving our understanding of gut immune maturation in food-producing animals is crucial for both human and animal health.IMPORTANCEIn commercial farms, newly hatched chicks may lack host-specific microbiota that help mature their gut immune system for lifelong health benefits. Here, introducing an avian segmented filamentous bacteria (SFB) to commercially sourced chickens orally at hatch accelerated SFB colonization of the ileum. Remarkably, SFB from layers were able to colonize broilers and enhance gut immune maturation, and this immunomodulation impacted the ability to increase intestinal and extraintestinal resistance to bacteria relevant to poultry and human health. With the antibiotic restrictions in animal production, strategies that will help mitigate infections are urgently needed. In summary, we developed a live prophylactic for newly hatched chicks to improve animal health and food safety. Due to the host specificity of SFB, our data highlight the importance of investigating the molecular mechanism of SFB interaction in their own host.

'The veterinary profession must condemn intensive pig farming'.

Steven McCulloch argues that the global intensive pig farming industry does not meet the welfare needs of pigs and that the veterinary profession should and must withdraw all support.

Characterization and monitoring of changes during lactation in the profile of multiple bioactive compounds of milk from grazing mares.

Mare milk has often been considered a food product with potential functional properties. However, the bioactive compound composition of mare milk, including vitamins and other minor bioactive compounds, as well as factors affecting this composition have scarcely been studied. Therefore, the present study aimed to characterize the changes during lactation in the content of water- and fat-soluble vitamins and total polyphenols, and the total antioxidant capacity of mare milk from semi-extensive farms. A total of 310 individual milk samples from 18 mares belonging to three commercial farms and 12 lactation times were analyzed. Ascorbic acid (vitamin C), thiamine (vitamin B1), riboflavin (vitamin B2), nicotinic acid and niacinamide (vitamins B3), pantothenic acid (vitamin B5), pyridoxal and pyridoxine (vitamins B6), folic acid (vitamin B9), cyanocobalamin (vitamin B12), tocopherols and tocotrienols (vitamin E) and retinol and retinyl esters (vitamin A) were quantified using liquid chromatography. Total polyphenols and antioxidant capacity assays were analyzed using spectrophotometry. The concentration of most bioactive compounds tended to decline as lactation progressed, with the exception of polyphenols and the total antioxidant capacity that oscillated during lactation. On the other hand, the effect of the different semi-extensive management of the farms was only significant for vitamin B3 content. To the best of our knowledge, the present study provides the most in-depth description of the vitamin profile of mare milk as well as new insights into polyphenol content and antioxidant capacity of mare milk. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

How mariculture expansion is dewilding the ocean and its inhabitants.

The world's oceans are largely free from intensive farming, but momentum to intensify and expand mariculture-the cultivation of aquatic organisms in the ocean-is growing. Despite optimism that mariculture will create economic and nutritional benefits for humans, it can also generate a host of risks, including environmental degradation, harms to wildlife integrity and welfare, captivity effects, and shifts in how humans view the nonhuman world. Collectively, we refer to these four types of risks as "dewilding." In this systematic review, we searched Scopus and Web of Science for recent literature documenting mariculture's dewilding impacts to organize and collate this evidence under one unified framework. We find that mariculture's dewilding impacts are consistently documented, though often in isolation, and that captivity and conceptual dewilding impacts are recognized as potential harms far less than impacts on the environment and wildlife. Future work examining mariculture's dewilding impacts will be paramount to guiding human decision-making and activity going forward.

The Effect of Synbiotics and Probiotics on Ochratoxin Concentrations in Blood and Tissues, Health Status, and Gastrointestinal Function in Turkeys Fed Diets Contaminated with Ochratoxin A.

The aim of this study was to evaluate carcass quality and analyze gastrointestinal functional status, ochratoxin A (OTA) accumulation in tissues and organs, and the health status of turkeys fed diets contaminated with OTA and supplemented with synbiotic preparations in comparison with commercial probiotic feed additives. The research involved 120 female BIG 6 turkeys, divided into six treatment groups (five replicates, four birds per replicate). Wheat naturally contaminated with OTA (662.03 μg/kg) was used in turkey diets. Turkeys in group 1 received an OTA-contaminated diet without additives. Groups 2 and 3 received 0.4 g/kg of probiotic preparation BioPlus 2B or Cylactin. Groups 4, 5, and 6 received 0.5 g/kg of synbiotics S1, S2, or S3. The following parameters were monitored: growth performance, carcass quality, gastrointestinal tract structure and digesta pH, health status, and concentrations of OTA in the blood and tissues of turkeys. The study found no significant differences in the growth performance and carcass quality of turkey. However, the introduction of probiotics or synbiotics into OTA-contaminated feed mixtures resulted in a reduced pH of the digesta in certain sections of the turkey digestive tract (p < 0.05). Additionally, the tested synbiotic additives significantly reduced liver weight in turkeys at weeks 6 and 15 (p < 0.05). The addition of probiotic and synbiotic preparations based on lactic acid bacteria strains, inulin, and S. cerevisiae yeasts to OTA-contaminated diets in commercial turkey farming may improve health status (p < 0.05) and reduce mycotoxin accumulation in organs and tissues of poultry (p < 0.05).

Molecular characterization of reassortant infectious bursal disease virus (IBDV) strains of genogroup A3B1 detected in some areas of Britain between 2020 and 2021

Infectious bursal disease virus (IBDV) causes a major immunosuppressive disease of chickens. As part of ongoing epidemiological surveillance for IBDV, the hypervariable region (HVR) of the VP2 capsid gene encoded by segment A, and a region of the VP1 polymerase gene, encoded by segment B, were sequenced from 20 IBDV-positive bursal samples obtained in 2020 and 2021, from 16 commercial British broiler farms. Birds had received a live IBDV vaccine at 17–22 days of age, and samples were obtained at 25–55 days of age. Of the 16 farms, none contained very virulent (vv) strains, one contained a classical virulent strain, two contained vaccine strains, and five contained sequences of reassortant strains with a vv segment A and a non-vv segment B belonging to genogroup A3B1. In eight of the farms, we identified the sequences of both genogroup A3B1 reassortant strains and vaccine strains in the same samples. Therefore, the majority of the farms (13/16 (81%)) contained genogroup A3B1 reassortant viruses. Of the flocks containing reassortant strains, 5/13 (38%) had HVR mutations Q219L, G254D, D279N, and N280T, consistent with a recently described Western European clade, but the rest had other mutations or no mutations, demonstrating that multiple clades were present in the samples. Taken together, vv strains were not detected, but reassortant strains predominated in the farms, which belonged to different clades, and were frequently found together with vaccine strains.

Effect of Different Weed Management Practices on growth and yield in Potato at Khumaltar, Lalitpur, Nepal

Potato (Solanum tuberosum L.) is the fifth most important crops in the world in terms of production with an estimate of 375 million metric tons. In Nepal, potato ranks second in terms of production and sixth in terms of area coverage. Potato production is affected by weed infestation. Weeds compete with crops for nutrients, water and other resources, which eventually reduce the crop yield. The critical period of crop-weed competition for potato is 25-40 days from planting depending on the climatic-zone and planting seasons. The most common method of weed control in Nepal is manual method, while herbicides are uses in less areas. Weeds are considered as the major problem in conventional and organic farming, due to lower yields by weeds and higher costs of labor for weed control. Mulching is one of the best and safest practice for effective weed management in potato due to which micro-climate manipulation is developed. Metribuzin is widely used herbicides in potato that controls a range of broadleaf weed species and suppresses many grassy weeds. To study the performance of different weed management practices in potato ten treatments were set up viz., three herbicides (pendimethalin@ 1.0 kg a.i ha-1, metribuzin@ 0.5 kg a.i ha-1 and atrazine @ 0.25 kg a.i ha-1) constituting seven treatments, one mulching with rice straw, one treatment with two manual hoeing, and another treatment with no any weed management practices as control.The treatments were laid out in RCB design with 4 replications. The potato variety Janak Dev was planted on 12th Feb 2021 and 23rd Jan 2022 with tuber rate of 4.1 t.ha-1 with row spacing of 60 cm and plant to plant 20 cm in a plot size of 4 x 3 meter in flat bed. Growth parameters such as plant height, plant fresh and dry biomass were recorded. Data on weed density, weed dry biomass, and tuber yield were taken.The economics of the different treatments were calculated. Among the treatments metribuzin@ 0.25 kg a.i ha-1 as pre-emergence proved effective in controlling weeds showing highest weed control efficiency (200.7 %) and tuber yield (32.79 t ha-1) . However, the treatment rice straw mulch @ 6 t ha-1 produced the highest tuber yield (38.31 t ha-1), 73.4 % increased yield over control, weed control efficiency(147.1%), economic benefit (NRS 13,40,850), benefit cost ratio (4.93).The treatment rice straw mulch @ 6 t ha-1 showed superiority among the different weeds management practices.