Adsorption into biochar-derived materials and mycoremediation are promising technologies for removing dyes from solid and liquid matrices. This study presents a combined treatment with adsorption into wood-chip biochar and mycodegradation under solid-state fermentation by Trametes villosa for removing the leather-dye Acid Blue 161. In the first stage, untreated wood-chip biochar, NaOH–depolymerised biochar and KMnO4–depolymerised biochar were assessed for their dye removal efficiency by adsorption. KMnO4–depolymerised biochar exhibited the highest adsorption (85.1 ± 1.9%) after 24 h of contact. KMnO4–depolymerisation modified some physical and chemical properties on the untreated wood-chip biochar, increasing the surface area (50.4 m2 g–1), pore size (1.9 nm), and presence of surface functional groups. Response surface methodology coupled with a Box–Behnken design was used to optimise the AB161 adsorption into the KMnO4–depolymerised biochar. The optimised conditions, pH 3.0, dye concentration 100 mg L–1 and sorbent dosage 2 g L–1, led to a higher dye removal efficiency by adsorption (91.9 ± 1.0%). In a second stage, the wood-chip biochar supplemented with nutrients (1% malt extract and 0.5% peptone) was employed as a solid matrix for growing T. villosa and regenerating the dye-saturated material. After 15 days, T. villosa was able to grow (86.8 ± 0.8%), exhibit laccase activity (621.9 ± 62.3 U L–1), and biodegrade (91.4 ± 1.3%) the dye adsorbed into the KMnO4–depolymerised biochar. Finally, the mycoregenerated biochar was reutilised in a new cycle of adsorption reaching 79.5 ± 2.0% of dye removal efficiency by adsorption. This study revealed the potential of the combined treatment and is an initial assessment for developing commercial alternatives for treating leather industry wastewaters.