Buildings are one of the leading sources of carbon emissions in the world. Most of the carbon emissions are released during the operation phase of the building. It is essential for buildings to provide thermal and visual comfort for the users. In the case of existing buildings, it is necessary to offer retrofit solutions so that the operational carbon emissions can be reduced without compromising on the other essential factors. In this study a Multi-Objective Optimization (MOO) of passive design strategies was conducted for a commercial laboratory in India situated in a moderate climate zone. The design variables considered for the study are wall and roof insulation, glazing material, window-wall ratio (WWR), depth of shading device and the number of shading devices used. The objective functions are: 1. reduced energy use intensity and operational carbon emissions, 2. increased thermal comfort hours and 3. increased daylight autonomy. Rhinoceros and grasshopper software along with Ladybug and Honeybee plug-ins were used for the study which resulted in 1296 iterations. MOO technique namely Pareto front optimization was used to optimize the objective functions. Out of 1296 solutions (excluding base case), 72 solutions were non-dominated. Two methods are described in the study to identify the recommendations for retrofit. The first method describes a Heuristic method of selection using Design Explorer recommending 5 good solutions. In the second method a factor is evolved to identify the 5 best solutions in sequential order. The overall study recommends the use of EPS insulation for the RCC roof, WWR of 20% on all sides, 3 horizontal shading devices of depth 0.75 m for all window openings. When compared with the base case scenario, this solution minimizes the EUI by 3.7%, maximizes average TCH by 106.6% and maximizes average DA by 66.9%. The overall operational carbon emissions are reduced by 7095.6 kgCO2.