Serpentine nozzles more easily deform and damage due to high-temperature airflow because of their multi-bend channel configuration. Therefore, film cooling technologies should be applied to serpentine nozzles. Complex pressure gradients in serpentine nozzles lead to different film cooling characteristics from combustion chambers, turbine blades, and other exhaust systems. This study experimentally investigated the effects of complex pressure gradients and film hole inclination angles on a serpentine nozzle’s film cooling effectiveness (FCE) using pressure-sensitive paint (PSP). The flow mechanisms were obtained via numerical simulations. The experimental nozzle design needs to consider the needs of PSP observations and the normal flow of serpentine nozzles. However, the complex structure of serpentine nozzles makes those increasingly difficult. The studied pressure gradients include the pressure gradient mutation (PGM), strong adverse pressure gradient (SAPG), weak adverse pressure gradient (WAPG), and favorable pressure gradient (FPG). The results show that complex pressure gradients change the coolant coverage and adhesion and may induce a recirculation zone in the SAPG region. This affects the development of the vortex construction and leads to different FCE distributions. The FCE under the effects of the PGM is the largest, followed by the SAPG, WAPG, and FPG. A larger inclination angle weakens the coolant adhesion but may enhance its downstream and lateral flow treads. This is coupled with the effects of complex pressure gradients, where larger inclination angles bring better film cooling effects in some cases. After accounting for the four blowing ratios, the area-averaged FCEs under the effects of the SAPG, WAPG, and FPG are 28.9 %, 42.8 %, and 52.3 % lower than that under the PGM, respectively. The area-averaged FCEs under the conditions α = 45° and 60° are 6.6 % and 10 % lower than that under α = 30°.