<i>Combretum glutinosum</i> and <i>Mitragyna inermis</i> are two plants used in Benin as a dewormer and antibiotic in traditional human and veterinary medicine. This study aimed to evaluate the anthelmintic, antimicrobial and antioxidant activities of the both plants tannin extracts. The extracts were tested <i>in vitro</i> on <i>Haemonchus contortus</i> larvae and worms and on the growth of 11 reference strains by agar medium diffusion method. Their chemical compositions were determined by UPLC-DAD-ESI-MS. It was found that total tannins extracted from plant leaves showed a strong inhibition on <i>H. contortus</i> larvae and adult worms compared to the negative control. Concerning the antimicrobial activity, <i>M. inermis</i> extract had an effect only on <i>Pseudomonas aeruginosa</i> with a MIC of 2.5 mg/mL. <i>C. glutinosum</i> extract inhibited the growth of most microbial strains with MIC values ranging from 1.25 to 20 mg/mL. The DPPH test showed that the extracts of <i>C. glutinosum</i> (IC<sub>50</sub> = 8.04 µg/mL) and <i>M. inermis</i> (IC<sub>50</sub> = 11.21 µg/mL) have good antioxidant activity and these results are confirmed by the FRAP method. Four (4) compounds could be identified in the tannin extract of <i>C. glutinosum</i> and could explain its activities. The results obtained from this work revealed that the tannins extracted from <i>C. glutinosum</i> showed better anthelmintic, antimicrobial and antioxidant activities compared to the extracts from <i>M. inermis</i>.
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