Combined Effect Of Salt Research Articles

Effects of water salinity and temperature on oil-in-water emulsions stabilized by a nonionic surfactant

The goal of this work is to understand the effect of water salinity of three salts- NaCl, CaCl2, and MgCl2- on oil-in-water (O/W) emulsions stabilized by nonionic surfactants such as Tergitol 15-S-7 (T15S7). The study investigates not only the impacts of mixing speed, water salinity, and temperature but also the combined effects of salt and temperature on emulsion stability. All emulsions were created using ExxsolTM D110 and distilled water, with a surfactant concentration of 0.050 % wt. in the water phase. Two oil fractions (10 % and 25 % by volume) were studied, and oil and water separation interfaces were measured using a state-of-the-art apparatus, which can record and scan videos of free liquid separation. According to the experimental findings, at concentrations higher than the surfactant’s Critical Micelle Concentration (CMC), neither of the divalent salts affects the surface tension of the aqueous solution. Divalent ions, on the other hand, were found to decrease the surfactant’s effectiveness at concentrations lower than the CMC. Conversely, monovalent ions had no effect at low concentrations but further reduced the surface tension at large concentrations. All salts, however, had no effect on the volume of the emulsion for more than 4 h at 25 °C, however at 70 °C, 100 % separation happens in a matter of seconds. Decrease in mixing speed from 2500 RPM to 800 RPM caused the emulsion volume to decrease by at least 72 %.

Effect of Salinity and Substrate on the Emergence and Growth of Propagules of the Mangrove Species Rhizophora racemosa in the Sassandra-Dagbego Ramsar Complex, Côte d’Ivoire

This study aimed to evaluate the behaviour of seeded propagules of Rhirophora racemosa (R. racemosa) on different substrates and under different salinity levels. Three substrates including sand, mud and a mixture of the two were tested together with tree salinity levels (low 5%, moderate 10% and high 25%). R. racemosa seedlings were more likely to emerge and grow under moderate and low salinity conditions. The propagules had significant early growth in mud compared to sand and mud-sand mixture. The combined effect of salt and substrate influenced significantly propagules performance in nursery (p<0.001). High propagules emergence and growth were observed in the combination mud and salt treatments as compared to sand and san-mud mixture substrates. These results provide valuable information for the management and restoration of mangroves, highlighting the optimal environmental conditions for the successful regeneration of this species.

Corrosion behavior and mechanism of X80 steel in silty soil under the combined effect of salt and temperature.

In this study, X80 pipeline steel was embedded in silty soil with different salinities and subjected to corrosion at a constant temperature for 24 h before electrochemical testing. The effect of soil medium, temperature, and salt content on the kinetics of corrosion behavior of X80 steel was analyzed. Furthermore, the compositions and structures of the corrosion products were analyzed by X-ray photoelectron spectroscopy and scanning electron microscopy. Based on the results, the anodic dissolution reaction mechanism of X80 steel in silty soil was determined, the differences in the corrosion process caused by different soil systems were comprehensively contrasted, and the impact of the migration process of heterogeneous silty soil on corrosion behavior under different conditions was systematically explored. Comparative analysis revealed that chloride ions possess strong adsorption ability at temperatures above freezing point and that more oxidized substances are present in the deposited layer on the surface of corroded steel, which facilitates the occurrence of corrosion under deposition. At temperatures below freezing point, the sulfate ions present in the pore solution contribute to crystallization-induced expansion and lead to swelling and deformation of the soil, rendering the X80 steel more prone to corrosion in sulfate corrosion environments.

Morphological characteristics, biomass accumulation and gas exchange of an important species native for restoration in Semi-arid Brazilian areas affected by salt and water stress

The Aspidosperma pyrifolium Mart. & Zucc. is one of the main species of Caatinga biome, a seasonally dry tropical forest in Brazilian Semi-arid, very prominsing for the restoration of degraded or desertified areas. The combined effects of salt and water stress on morphological dynamics, biomass accumulation and gas exchange in A. pyrifolium were investigated. The experiment was conducted in state of Pernambuco, Brazil. A completely randomised design was adopted, where the plants were submitted to four water regimes based on a percentage of the reference evapotranspiration (ET0): 25%, 50%, 75% and 100%.ET0, and four salinity levels, using NaCl: 0.2 (control), 1.8, 2.82 and 3.93 dS m−1. Biometric, biomass and gas exchange data were obtained, and water use efficiency, momentary and intrinsic water use efficiency were calculated. The water deficit affected stem diameter, and stem and root biomass. Gas exchange showed a reduction for an increase in salt and water stress. Momentary and intrinsic efficiency were maximized under a water regime of 65%.ET0 and an electrical conductivity of 1.51 dS m−1, and 67%.ET0 and 1.75 dS m−1, respectively. It is concluded that the species A. pyrifolium changes the biomass partitioning between the plant structures to compensate the salt and water stress.

Biostability of Facade Systems

Protection of buildings and structures made of stone materials from biological corrosion is relevant for all groups of buildings, especially in wet and wet modes of operation of external enclosing structures and in conditions of pollution from industrial emissions. The presence of salts in raw materials, mixing water and technological additives to improve the properties of the mixture in the manufacture of building materials, masonry and plaster mortars causes the transfer of salts to the facades of buildings. Due to the hygroscopic effect, salts attract atmospheric moisture, and waterlogged material is a favorable environment for the development of spores of various types of filamentous fungi. The combined effect of salt and biological corrosion reduces the strength properties of materials. Destruction of paint and plaster layers disrupts the appearance of building facades. The study investigated the biological resistance of samples of facade plastering systems to the action of test cultures of filamentous fungi. The fungicidal and fungicidal properties of facade plastering systems of various compositions have been studied. The influence of different types of primer, filler and color on fungal resistance, fungicide and physical and mechanical properties of facade coatings was revealed. It was revealed that multilayer facade systems, consisting of materials with high vapor permeability, water-repellent properties and biostability, provide joint protection against salt exposure and the influence of biological pollution. The aesthetic qualities of the facade surface are ensured by decorative plastering or painting.

Paclobutrazol induced non-enzymatic antioxidants and polyamine levels in soybean plants grown under salinity stress

Paclobutrazol (PBZ) is a member of the triazole family of plant growth regulators and is known to protect crops from environmental stresses such as salinity and drought. It plays a role in maintaining water balance, photosynthesis capacity and enhancing antioxidant enzyme activities. Despite the well-documented effects of PBZ on antioxidant defence in soybean plants, the changes in non-enzymatic antioxidants and endogenous polyamines in the PBZ-treated plants grown under salinity stress are still not studied. Herewith, the study aimed to clarify the effects of PBZ on these molecules. For this purpose, the combined effect of salt (250 mM NaCl) and three different concentrations of PBZ (5, 10 and 20 ppm) on soybean (Glycine max L.) plants were studied. Proline, hydroxyl radical (OH.) scavenging capacity, non-enzymatic antioxidants (anthocyanin, flavonoid, phenolic and free thiol-containing compounds) and polyamines spermine, spermidine, and putrescine were investigated in the treated plants. We found that the PBZ (10 ppm) treatment was the most effective concentration to counteract oxidative stress due to NaCl in soybean seedlings. It significantly increased hydroxyl radical scavenging activity, proline, total phenolics, flavonoids, and conjugated polyamine content compared to those found in salt-treated plants only. For the first time, our study showed that PBZ could induce the non-enzymatic antioxidant defence in soybean plants grown under salt stress conditions.

Cobalt+Salt-Stressed Salvia officinalis: ROS Scavenging Capacity and Antioxidant Potency

Salvia officinalis L. (Lamiaceae) is one of the most widespread herbal species used in the food processing industry and for culinary and medicinal purposes. This work aimed to investigate changes in plant growth, water content, lipid peroxidation, H2O2, proline, and enzymes related to reactive oxygen species (ROS) detoxification including superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Phenolic contents and antioxidant capacity values such as ferric ion reducing antioxidant power (FRAP), cupric ion reducing antioxidant capacity (CUPRAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging were studied under stress conditions of salt, cobalt and a combination of the two. No significant differences were found in relative water content and chlorophyll fluorescence under salt, cobalt and their combination. However, the osmotic potential and relative growth rate were enhanced with salt+cobalt compared to salt-treated plants. Salt and cobalt individually stimulated high antioxidant activity. High APX and GR activities were associated with the high proline accumulation in the sage plants under the combined effect of salt+cobalt. The combination decreased lipid peroxidation (TBARS), while H2O2 content was increased. This increase with the combined salt+cobalt effect may be associated with the decrease in CAT activity. Moreover, a strong correlation was found between TPC and TF content and antioxidant capacity measured via FRAP, CUPRAC and DPPH. The TPC, TF and antioxidant capacity values also increased under the salt+cobalt combination, suggesting an increase in antioxidant content in the sage leaves. Therefore, the combination of salt and cobalt improved the stress tolerance of S. officinalis.

Combined effects of salt, cyclic wetting and drying cycles on the physical and mechanical properties of sandstone

Salt weathering has considerable effects, and it has recently become the subject of interest among researchers and engineers, especially in terms of sandstone heritage buildings and sandstone monuments. However, the impacts of salt weathering on sandstone after wetting-drying cycles have been neglected in the literature. Under the conditions of the long-term or gradual underground seepage of water into sandstone heritage buildings and monuments, salt accumulation and recrystallization occur in sandstone when the rate of evaporation is sufficiently high, and they reduce rock stability. In view of this problem, experiments subjecting sandstone to up to 50 wetting-drying cycles were conducted using water and solutions containing concentrations of 4%, 6% or 8% magnesium sulfate (MgSO4). The physical and mechanical properties were tested after different wetting-drying cycles. The results show that the wetting-drying cycles impacted the sandstone samples soaked in a salt solution more than the samples that were soaked in only water. Thirty cycles is the threshold number in terms of changes in P-wave velocity, thermal conductivity and tensile strength. A correlation analysis was conducted, and it showed that both color lightness and thermal conductivity are good parameters for evaluating tensile strength. The results contribute to the evaluation process and protection of sandstone heritage buildings and monuments against salt weathering.

Pressure-Stimulated Supercrystal Formation in Nanoparticle Suspensions.

Nanoparticles can self-organize into "supercrystals" with many potential applications. Different paths can lead to nanoparticle self-organization into such periodic arrangements. An essential step is the transition from an amorphous state to the crystalline one. We investigate how pressure can induce a phase transition of a nanoparticle model system in water from the disordered liquid state to highly ordered supercrystals. We observe reversible pressure-induced supercrystal formation in concentrated solutions of gold nanoparticles by means of small-angle X-ray scattering. The supercrystal formation occurs only at high salt concentrations in the aqueous solution. The pressure dependence of the structural parameters of the resulting crystal lattices is determined. The observed transition can be reasoned with the combined effect of salt and pressure on the solubility of the organic PEG shell that passivates the nanoparticles.

Combined effect of salt and drought on boron toxicity in Puccinellia tenuiflora

Boron toxicity is a worldwide problem, usually accompanied by salt (NaCl) and drought. The combined stresses may induce complex toxicity to the plant. The aim of the present study was to investigate how the combined stresses of salt and drought affect B toxicity in plants. Puccinellia tenuiflora seedlings were planted in vermiculite. A three (B) × three (salt) × three (drought) factorial experiment (for a total of 27 treatments) was conducted. After a 30-day cultivation, plants were harvested to determine dry weight and the concentrations of B, Na+, K+, Ca2+, and Mg2+. Plant growth was inhibited by B toxicity, which was alleviated by salt and drought. B stress enhanced B uptake and transport of the plant, which was inhibited by salt and drought. B stress had a little effect on K+ and Na+ concentration and caused Ca2+ and Mg2+ accumulation in the plant. Salt addition increased Na+ concentration and inhibited Ca2+ and Mg2+ accumulation. Drought addition inhibited Na+ accumulation and enhanced Ca2+ and Mg2+ accumulation. The combined stresses of salt and drought had a greater alleviation on the inhibition of dry weight caused by B than individual salt and drought. Besides, the combined stresses of salt and drought also enhanced B uptake and inhibited B transport. The results indicate that salt, drought, and the combined stresses of salt and drought all can alleviate B toxicity in P. tenuiflora, the main mechanism of which is the restriction of B and Na+ uptake caused by salt and drought. The combined stresses of salt and drought have a greater effect on B toxicity than individual salt and drought.

Hydroponic lettuce yields are improved under salt stress by utilizing white plastic film and exogenous applications of proline

Abstract Greenhouse crops are often affected by salinity due to water quality decay associated with crop fertilization and irrigation managements as well as the fact that greenhouse environment may become even hotter during summer time. Thus, combined effects of salt, heat and light stresses can affect plants grown in greenhouse. This research work addressed the combined effect of white greenhouse covering film and foliar proline spray application to reduce the detrimental effects of salinity on two cultivars of lettuce (Lactuca sativa cv. Teide and cv. Impulsion) grown on a floating system. Five different experiments were conducted in two twin greenhouses covered with plastic films characterized by different light permeability. The experiments aimed at identifying the most suitable nutrient solution (exp. #1), and assessing how the effects of mild salinity (0–15 mM NaCl) would be alleviated by the greenhouse covering film (exp. #2, #3, #4 and #5) and foliar proline spray application (0–15 μM) (exp. #4 and #5). Results showed that the white covering film changed the spectral light intensity and decreased the Photosynthetically Active Radiation (PAR) of the light transmitted causing a delay in the plant growth and leaf chlorophyll content. Although salinity negatively affected plant growth and leaf photosynthesis of both cultivars, using the white film partially mitigated the influence of salt stress. The beneficial effects of the white film on salt stress mitigation were more evident during summer and in the heat sensitive genotype (cv. Teide) in terms of greater total and marketable yield as compared to control conditions. Exogenous application of foliar proline (up to 5 μM) increased the yield under control condition and enhanced the plant response to salinity. Overall, for summer cultivation of cv. Teide, in presence of saline water (15 mM NaCl), the combination of both white covering film and proline application enabled to preserve efficiently the plant growth and final yield.

Factors influencing seed germination of the pastoral plant Retama raetam subsp. bovei (Fabaceae): interactive effects of fruit morphology, salinity, and osmotic stress

The aim of this work was to study the morphology of retama seeds and the combined effects of salt and water stress on the seeds germination of four Tunisian populations of Retama raetam. Seeds were harvested from Bouhedma National Park, Meknassi, Oueslatia, and Rtiba. In this study we were interested in evaluating different parameters of germination in presence of the salinity and osmotic stress. The results revealed that this species can withstand salinity up to 15 g/l of salt (the germination percentage of the Rtiba population reached 42%) and it can also tolerate severe drought resulted in significant doses of PEG6000 (germination percentage reached 29% to the water potential of –1.6 MPa of Oueslatia population). The morphological traits of the seeds were measured to find a relationship between the size of the seeds and their germination capacity under salinity and osmotic stress. Results have shown that for the correlation between morphological traits of seeds and the germination percentage, the seed weight is a major factor in promoting germination under stress and confirmed that saline water and sprouting inhibition have impact on the small caliber of seeds.

Role of 24-Epibrassinolide, Putrescine and Spermine in Salinity Stressed Adiantum capillus-veneris Leaves

In the present investigation, the effects of 24-epibrassinolide and polyamines (putrescine and spermidine) on antioxidant enzymes (superoxide dismutase, catalase and guaiacol peroxidase), antioxidants (phenols and proline), lipid peroxidation, proteins and photosynthetic pigments in the Adiantum capillus-veneris under salt stress were studied. 24-epibrassinolide and polyamine treatment alone or in combination with salt stress modulate the activities of antioxidant enzymes. Modest decrease in proline content was observed for salt treatment alone. However, the combination of 24-epibrassinolide and polyamines with salt stress caused major increase in proline content over salt stress alone. The combined effect of salt with 24-epibrassinolide increased the phenolic content. Major enhancement in phenolic content was observed for 150 mM NaCl+10−8 EBL. A remarkable decrease in malondialdehyde content was observed in leaves treated with 24-epibrassinolide, putrescine and spermidine with/or without salinity stress. Proteins and photosynthetic pigments did not show any remarkable change under salt stress whereas, treatment of 24-epibrassinolide and polyamines enhanced the titers of protein and photosynthetic pigments of leaves with/or without salt stress. An increase in carotenoid content was observed with salt (150 and 300 mM) stress, which further improved remarkably by supplementation of polyamines and 24-epibrassinolide. Data presented here is one of the first detailed analysis of application of EBL, Put and Spd on the antioxidant system of Adiantum under salt stress.

Effect of Salt, Smoke Compound, and Temperature on the Survival of Listeria monocytogenes in Salmon during Simulated Smoking Processes

The objectives of this study were to examine and develop a model to describe the survival of Listeria monocytogenes in salmon as affected by salt, smoke compound (phenol), and smoking process temperature. Cooked minced salmon containing selected levels of salt (0%, 2%, 4%, and 6%) and smoke compound (0, 5, 10, and 15 ppm phenol) were inoculated with a 6-strain mixture of L. monocytogenes to an inoculum level of 6.0 log(10) CFU/g. The populations of L. monocytogenes in salmon during processing at 40, 45, 50, and 55 degrees C that simulated cold- and hot-smoking process temperatures were determined, and the effects of salt, phenol, and temperature on the survival of L. monocytogenes in salmon were analyzed and described with an exponential regression. At 40 degrees C, the populations of L. monocytogenes in salmon decreased slightly with inactivation rates of <0.01 log(10) CFU/h, and at 45, 50, and 55 degrees C, the inactivation rates were 0.01 to 0.03, 0.15 to 0.30, and 2.8 to 3.5 log(10) CFU/h, respectively. An exponential regression model was developed and was shown to closely describe the inactivation rates of L. monocytogenes as affected by the individual and combined effects of salt, phenol, and smoking process temperature. Temperature was the main effector in inactivating L. monocytogenes while salt and phenol contributed additional inactivation effects. This study demonstrated the inactivation effects of salt, smoke compound, and temperature on L. monocytogenes in salmon under a smoking process. The data and model can be used by manufacturers of smoked seafood to select concentrations of salt and smoke compound and alternative smoking process temperatures at 40 to 55 degrees C to minimize the presence of L. monocytogenes in smoked seafood.

Effects of Phosphates and Salt in Ground Raw and Cooked Farmed Cod (Gadus morhua) Muscle Studied by the Water Holding Capacity (WHC), and Supported by 31P‐NMR Measurements

A model system consisting of ground farmed cod muscle (80%, w/w) and added brine (20%, w/w) with different content and combinations of salt (0% and 3% in brine) and phosphorus compounds (mono-, di-, tri- and hexametaphosphates; 0% and 3% in brine) was used to simulate industrial brining of muscle foods. Individual phosphorus component concentrations and breakdown as function of time (0, 23 h) were analyzed using (31)P-NMR spectroscopy. The effects of salt and phosphate on water holding capacity (WHC) were measured at similar sampling times, and interrelations between phosphorous components determined by NMR and WHC were established. Addition of salt led to a significant increase (+18%) in WHC, and the combined effect of salt and phosphates was even more pronounced (+29%). The positive effect of triphosphate and salt on WHC was also seen after cooking (+36% in raw and +41% in cooked cod muscle, relative to control), although NMR analysis showed a rapid breakdown of di- and triphosphates.

Sorption of a Fluorescent Whitening Agent (Tinopal CBS) onto Modified Cellulose Fibers in the Presence of Surfactants and Salt

The combined effect of salt (10 mmol L(-1)) and surfactants on the sorption of the fluorescent brightener 4,4'-distyrylbiphenyl sodium sulfonate (Tinopal CBS) onto modified cellulose fibers was studied. Sorption efficiencies with both cationic and anionic surfactants were evaluated. Emission spectroscopy was used for quantitative analysis since Tinopal has an intense fluorescence. The sorption efficiency of the brightener is greater for solutions containing a cationic surfactant (DTAC) below the critical micelle concentration (cmc), while for an anionic surfactant (SDS) above its cmc the efficiency is greater. The profile of the sorption isotherms were interpreted in terms of the evolution of surfactant aggregation at the fiber/solution interface. Salt influences the efficiency of the Tinopal sorption on the modified cellulose fibers either because it decreases the cmc of the surfactants or because the ions screen the surface charges of the fiber which decreases the electrostatic interaction among the charged headgroup of the surfactant and the charged fiber surface.

Effects of salt, acid, and MSG on cold storage survival and subsequent acid tolerance of Escherichia coli O157:H7

The combined effects of salt, monosodium glutamate (MSG), and pH on cold storage survival and subsequent acid tolerance of Escherichia coli O157:H7 were determined. Cold storage survival was evaluated in tryptic soy broth (TSB) with combinations of pH (7.2, 5.0, or 4.0), MSG (0, 0.5, 1%) and salt (0, 2, 4%). Survival through 21 d at 5°C and acid tolerance in simulated gastric fluid were evaluated weekly. In separate experiments, strains were tested individually for the effect of growth in the presence of MSG on subsequent acid resistance and for the ability of MSG to impact growth under acid conditions. The impact of salt on cold storage survival was greater at pH 4.0 and 7.0 compared to pH 5.0. MSG did not enhance cold storage survival. The presence of MSG alone enhanced acid tolerance following cold storage at pH 5.0 and 7.2 compared to control cells. At pH 4.0, MSG alone enhanced acid tolerance compared to control cells following 21 days cold storage. Overnight growth in TSB containing MSG did not affect subsequent acid tolerance in acidified TSB (pH 2.0). The presence of MSG in TSB (37°C) did not enable growth at lower pH.

Kinetic growth parameters of different amylolytic and non-amylolytic Lactobacillus strains under various salt and pH conditions

Four Lactobacillus species were studied for their ability to grow at high NaCl concentrations and different initial pH values. Lactobacillus plantarum 541 and A6 could ferment glucose and produce lactic acid in the presence of 8% salt in the medium. For strain 541, the specific rate of lactate production ( q lac) and the yield of lactic acid relative to substrate ( Y p/ s ) remained constant, whereas the yield of biomass relative to substrate ( Y x/ s ) decreased up to 6% salt. In contrast, for strain A6, Y p/ s decreased up to 6% salt whereas Y x/ s did not vary markedly. Combined effects of salt and pH studied through a factorial design did not show significant interaction between salt and pH. The pH was the dominant factor in glucose fermentation for both the strains. Considering overall performance, 4% salt and pH between 6.0 and 6.6 can be taken as appropriate conditions, for the use of both strains as starters in processes where higher salt concentrations are required.

Carotenoid Retention In Canned Pickled Jalapeño Peppers and Carrots As Affected by Sodium Chloride, Acetic Acid, and Pasteurization

ABSTRACT The combined effect of salt, acetic acid, and pasteurization temperature on the retention of carotenoids in canned pickled carrots and green jalapeño peppers was studied by a central composite design. The results were analyzed by response surface methodology. The carotenoid standards were obtained by open column chromatography and the quantitation was done by HPLC. Only the main carotenoids were quantified: α‐ and β‐carotene in carrots and α‐, β‐carotene, lutein, and violaxanthin in peppers. After analyzing the experimental results and the restrictions of the Mexican Regulations, 2% NaCl and 2% acetic acid concentrations were recommended. The optimal pasteurization conditions were 70 °C/12.45 min for carrots and 83 °C/5.2 min for peppers.

Combined effects of NaCl and raw meat pH on water-holding in cooked sausage with and without added phosphate

The intake of sodium in diets is of concern in many industrialized countries. Attempts have been made to lower sodium intake via meat products. The keeping quality, taste and water-holding/firmness of meat products have usually been impaired. The water-holding in cooked sausage was determined by a laboratory sausage method. Beef and pork with varying natural post-rigor pH-values (range: pork 5.50–6.12 and beef 5.60–6.48) were used as mixtures, and 0.5–2.5 % NaCl was used with or without added commercial sausage phosphate (2,5 g/kg determined as P 2O 5). The pH-values of raw batter increased to a level 0.0–0.7 units higher than the pH-values of the respective meat mixtures. The increase was higher in lower pH-values, with higher salt levels and with added phosphate. The cooking caused an additional increase of about 0.0–0.2 units, and the increase was again higher at lower pH-values. Maximum in water-holding was reached in 2.5% NaCl in all pH-values, both with and without added phosphate. The pH-value of meat raw materials for the maximum water-holding was ca. 6.3. The combined effect of salt and pH is important in high salt contents and low pH-values. By 2.5% NaCl , where the maximum in water-holding was obtained, raw meat pH has the largest effect, but in low NaCl contents, below 1.0%, only a minor effect in pH-values below 5.9, and above that, almost no effect. Approximately the same water-holding as with 2.5% NaCl in pH 5.7 can be reached with 1.5% NaCl in pH 6.1 and above. The effects of the variables were similar with pork and beef. It was concluded that when lowering the salt content in cooked sausages, the pH of the batter should be increased by using high-pH meat mixtures and/or pH-raising phosphates in order to reach a high enough level of water-holding.