Temporal diet patterns were studied for nine species of haplochromine cichlids in Lake Malawi, East Africa, using a combination of stable isotope and stomach content analyses. The results illustrate the complementarity of the two approaches in elucidating dietary differences. Evidence was found that benthic algal production contributed to the energy requirements of offshore fishes that occupy depths between 10 and 30 m. The importance of the lake fly Chaoborus edulis as a food source for demersal fishes was confirmed, supporting the recent hypothesis that deep demersal fish production is principally sustained through the pelagic food chain rather than from benthic detrital sources. Isotopic differences were observed among species with apparently similar diets, feeding behavior, and depth preferences, suggesting that important resource partitioning exists among Lake Malawi benthic haplochromine cichlids.