Combination Of Monensin Research Articles

Combination of monensin and erlotinib synergistically inhibited the growth and cancer stem cell properties of triple-negative breast cancer by simultaneously inhibiting EGFR and PI3K signaling pathways.

Cancer stem cells (CSCs) in triple-negative breast cancer (TNBC) are recognized as a highly challenging subset of cells, renowned for their heightened propensity for relapse and unfavorable prognosis. Monensin, an ionophoric antibiotic, has been reported to exhibit significant therapeutic efficacy against various cancers, especially CSCs. Erlotinib is classified as one of the EGFR-TKIs and has been previously identified as a promising therapeutic target for TNBC. Our research aims to assess the effectiveness of combination of monensin and erlotinib as a potential treatment strategy for TNBC. The combination of monensin and erlotinib was assessed for its potential anticancer activity through various in vitro assays, including cytotoxicity assay, colony formation assay, wound healing assay, transwell assay, mammosphere formation assay, and proportion of CSCs assay. Additionally, an in vivo study using tumor-bearing nude mice was conducted to evaluate the inhibitory effect of the monensin and erlotinib combination on tumor growth. The results indicated that combination of monensin with erlotinib synergistically inhibited cell proliferation, the migration rate, the invasion ability and decreased the CSCs proportion, and CSC markers SOX2 and CD133 in vivo and in vitro. Furthermore, the primary proteins involved in the signaling pathways of the EGFR/ERK and PI3K/AKT are simultaneously inhibited by the combination treatment of monensin and erlotinib in vivo and in vitro. The simultaneous inhibition of the EGFR/ERK and PI3K/AKT/mTOR signaling pathways by the combination of monensin and erlotinib exhibited a synergistic effect on suppressing tumor proliferation and cancer cell stemness in TNBC.

Effects of Monensin and Rapamycin Combination Therapy on Tumor Growth and Apoptosis in a Xenograft Mouse Model of Neuroblastoma.

Neuroblastoma is the most common pediatric solid tumor originating from the neural crest. New treatment options are needed to improve treatment outcomes and the survival of patients with neuroblastoma. Monensin is an ionophore antibiotic with antiparasitic, antibacterial, and anticancer properties isolated from Streptomyces cinnamonensis. The aim of this study was to investigate the therapeutic effects of single and combined monensin and rapamycin treatments on mTOR (mammalian target of rapamycin) signaling pathway-mediated apoptosis and tumor growth in an SH-SY5Y neuroblastoma cell xenograft model. Control, monensin, rapamycin, and monensin + rapamycin groups were formed in the xenograft neuroblastoma model obtained from CD1 nude mice, and tumor volumes and animal weights were recorded throughout the treatment. In xenograft neuroblastoma tumor tissues, apoptosis was determined by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) and cleaved-caspase 3 immunohistochemistry, and PI3K (phosphoinositide-3-kinase)/AKT/mTOR expression was determined by the immunohistochemistry and immunofluorescence methods. The combination of monensin and rapamycin was to reduce the growth of xenograft neuroblastoma tumor tissues, trigger apoptosis, and suppress the expression of PI3K/AKT/mTOR. A significant increase in apoptotic cell rate was demonstrated in the combination group, supported by cleaved-caspase 3 immunohistochemistry results. In addition, it was reported that the combination treatment regime triggered apoptosis by reducing the expression of phosphorylated PI3K/AKT/mTOR. Our preclinical results may be a precursor to develop new therapeutic approaches to treat neuroblastoma.

Monensin, an Antibiotic Isolated from Streptomyces Cinnamonensis, Regulates Human Neuroblastoma Cell Proliferation via the PI3K/AKT Signaling Pathway and Acts Synergistically with Rapamycin

Neuroblastoma is the most common extracranial childhood tumor and accounts for approximately 15% of pediatric cancer-related deaths. Further studies are needed to identify potential therapeutic targets for neuroblastoma. Monensin is an ionophore antibiotic obtained from Streptomyces cinnamonensis with known antibacterial and antiparasitic effects. No study has reported the effects of monensin on SH-SY5Y neuroblastoma cells by targeting the PI3K/AKT signaling pathway. The aim of this study was to investigate the antiproliferative effects of monensin alone and in combination with rapamycin in human SH-SY5Y neuroblastoma cells mediated by the PI3K/AKT signaling pathway. The effects of single and combination applications of monensin and rapamycin on SH-SY5Y cell proliferation were investigated by XTT, and their effects on the PI3K/AKT signaling pathway by RT-PCR, immunohistochemistry, immunofluorescence, and Western blotting. The combined effects of monensin and rapamycin on SH-SY5Y proliferation were most potent at 72 h (combination index < 1). The combination of monensin and rapamycin caused a significant decrease in the expression of P21RAS, AKT, and MAPK1 genes. Single and combined administrations of monensin and rapamycin caused a significant decrease in PI3K/AKT expression. Our results showed for the first time that monensin exerts an antiproliferative effect by targeting the PI3K/AKT signaling pathway in neuroblastoma cells. It is suggested that monensin and its combination with rapamycin may be an effective therapeutic candidate for treating neuroblastoma.

Monensin synergizes with chemotherapy in uveal melanoma through suppressing RhoA

Objective Uveal melanoma (UM) is the common primary cancer of the eye and new treatments are needed. Substantial evidence has shown that an antibiotic monensin is an attractive candidate for the development of anti-cancer drug. In this study, we investigated the potential of repositioning monensin for the treatment of UM in the pre-clinical setting. Materials and Methods Cellular activity assays were performed using multiple cell lines representing UM models with different cellular origins and genetic profiling and normal cells as control. Combination studies were performed using Chou-Talalay method. Mechanism studies were performed using immunoblotting and ELISA. Results Monensin was effective against all tested UM cell lines and less effective against normal fibroblast cells. Monensin induced G0/G1 arrest and thus decreased S phase, leading to UM cell growth inhibition. It also inhibited migration and induced apoptosis in UM cells. In addition, the combination of monensin and dacarbazine was synergistic in targeting UM cells. Our mechanistic studies showed that monensin specifically decreased activity of RhoA without affecting other small GTPases, such as Ras and Rac1. Consistently, monensin decreased phosphorylation of downstream effectors of RhoA signaling, including ROCK, MYPT1 and MLC. Rescue studies using RhoA activator calpeptin showed that calpeptin significantly abolished the inhibitory effects of monensin on RhoA activity, proliferation, migration and survival, confirming that RhoA is the target of monensin in UM cells. Conclusions Our study demonstrates that monensin is a potent inhibitor of UM and synergizes with chemotherapy, via suppressing RhoA activity and RhoA-mediated signaling. Our findings suggest that monensin may be a potential lead compound for further development into a drug for UM treatment.

Meat acceptability of crossbred bulls fed a high-grain feedlot diet with antimicrobials and a blend of organic trace minerals and yeast

Context Natural products, organic trace minerals and yeast have been studied for use as alternatives to antibiotics in improving meat quality and acceptability of bulls finished in feedlots. Aims This study was conducted to evaluate acceptability to consumers of meat from feedlot-finished bulls fed diets containing antimicrobials with or without a natural product based on organic trace minerals and live yeast. Methods Crossbred (European × Nellore) bulls (n = 24, age 24 ± 3 months, weight 385.5 ± 3.8 kg) were fed one of four diets: control, without additives; and three diets that included monensin (ionophore, at 30 mg/kg DM intake) alone or with virginiamycin (antibiotic, at 30 mg/kg DM intake) or with a supplement comprising organic minerals and live Saccharomyces cerevisiae (30 g/100 kg bodyweight). Mineral and microbiological analyses of meat were conducted, and consumer perception of the products, including effects of aging and length of display, was measured. Key results No differences in meat mineral levels were found among treatments (P &gt; 0.05). Consumer acceptability was not modified (P &gt; 0.050) by diet with respect to meat odour, flavour, tenderness and overall acceptability. Meat aged for 7 days had higher scores (P &lt; 0.001) than meat aged for 1 day for all studied attributes except odour (P &gt; 0.05). In the first 7 days of display, meat from bulls fed monensin or monensin + supplement blend had the highest visual acceptability scores (P &lt; 0.001). For all diets, visual acceptability decreased with display time after 5 days (P &lt; 0.001). During the first week of display, consumers showed greatest willingness to buy meat from the treatments with monensin or monensin + supplement blend (P &lt; 0.001). Conclusion A combination of monensin and mineral–yeast blend may be beneficial to meat acceptability and shelf life and promote consumer willingness to buy. Implications Combinations of organic mineral and yeast with conventional additives such as monensin are suitable as supplements to increase meat quality of cattle finished in feedlots and fed high-grain diets.

Kinetics of In Vitro Gas Production and Fitting Mathematical Models of Corn Silage

This study aimed at examining the effects of rumen inoculum of steers receiving different combinations of ionophore and probiotics in their diets on in vitro gas production of corn silage. The fitting of gas production was performed with five mathematical models and its kinetics was evaluated. Four crossbred steers (403.0 ± 75.5 kg body weight) with ruminal cannula were assigned to a 4 × 4 Latin square design. The additives used were Monensin sodium (Rumensin® 100, 3 g/day), Bacillus toyonensis (Micro-Cell Platinum® 109, 1 g/day) and Saccharomyces cerevisiae boulardii (ProTernative®20, 0.5 g/day). Additives were arranged into the following treatments, supplied daily into total mixed diet: (1) Monensin; (2) Monensin + B. toyonensis; (3) Monensin + S. boulardii; and (4) B. toyonensis + S. boulardii. The gas production data were fitted into the models of Gompertz, Groot, Ørskov, Brody, Richards, and Dual-pool Logistic. A perfect agreement between observed and predicted values in curves of accumulated in vitro gas production was observed in the Groot and Richards models, with higher coefficient of determination (R2 = 0.770 and 0.771, respectively), concordance correlation coefficient (CCC = 0.871 and 0.870, respectively), and root mean square error of prediction (RMSEP = 1.14 and 1.15, respectively). Evaluating the feed additives throughout the Groot model, the B. toyonensis + S. boulardii treatment presented higher VF (12.08 mL/100 mg of DM; p = 0.0022) than Monensin and Monensin + S. boulardii (9.16 and 9.22 mL/100 mg of DM, respectively). In addition, the fractional rate of gas production (k) was higher (p = 0.0193) in B. toyonensis + S. boulardii than in Monensin, not presenting a statistical difference (p &gt; 0.05) from the other two treatments. Additionally, with the time of beginning to gas production, the lag time (λ), was greater (p &lt; 0.001) with Monensin and Monensin + B. toyonensis than with Monensin + S. boulardii and B. toyonensis + S. boulardii. The combination of Monensin and probiotics (B. toyonensis + S. boulardii) resulted in better kinetics of degradation of corn silage, being that the Groot and Richards models had the best fit for estimates of the in vitro gas production data of corn silage tested with different feed additive combinations.

Effects of a blend of live yeast and organic minerals or monensin on performance of dairy cows during the hot season

The objective of this study was to evaluate the effects of feed additives on intake and digestibility of nutrients, milk yield and composition, feeding behavior, and physiological parameters of dairy cows during the hot season. Forty Holstein cows were assigned to a randomized block design experiment with a 2 × 2 factorial treatment arrangement to evaluate (1) control diet without inclusion of additives; (2) monensin (MON), 20 mg/kg diet dry matter sodium monensin (Rumensin; Elanco); (3) Milk Sacc+ (MS+), inclusion of 40 g/cow per d of Milk Sacc+ (a blend of live yeast and organic minerals, Alltech); and (4) combination of MON and MS+. The average temperature-humidity index throughout the experimental period was 73 ± 2.84 (standard deviation). The experiment lasted 11 wk, including 2 preliminary weeks for covariate adjustments. Cows fed MS+ increased dry matter intake (% body weight), milk yield, 3.5% fat-corrected milk, and solids yield, and cows fed MON had greater milk urea nitrogen content in comparison with counterparts. Feeding MS+ increased the intake of feed particles with size between 8 and 19 mm and decreased the intake of particles shorter than 4 mm compared with other treatments. Rumination time (min/d) and chewing time (min/kg of neutral detergent fiber) were lower for cows fed MS+. Physiologic parameters (i.e., heart and respiratory rates, and body temperature) were not affected by the treatments. Overall, the use of monensin did not differ from control, and Milk Sacc+ improved performance of cows.

Effects of supplemental phytomolecules on growth performance, carcass characteristics and liver abnormalities of finishing beef steers

ABSTRACT This study was conducted to evaluate the growth performance and carcass characteristics of finishing steers fed diets containing different feed additives: control (CTL; no feed additives); monensin [26 mg/kg dry matter (DM); MON]; a blend of cinnamaldehyde, eugenol, and capsicum (120 mg of a commercial product/kg DM; XT), or the combination of monensin and the blend of cinnamaldehyde, eugenol, and capsicum (26 mg/kg DM and 120 mg of a commercial product/kg DM, respectively; MON + XT). A total of 860 steers [initial body weight (BW) = 334 kg] were fed the basal diet (7% alfalfa hay and 93% concentrate; DM basis) containing the treatments during 154 days. Feed additives did not affect final BW, DM intake, average daily gain, and feed efficiency of finishing steers (P ≥ 0.12). Dressing percentage was lower for MON + XT than MON and XT (P = 0.01). Feeding XT tended to increase the percentage of the carcass with a small marbling score compared to MON (P = 0.09). The proportion of carcass grading premium choice tended to be greater for steers fed MON compared to XT (P = 0.07). The feed additives evaluated herein did not affect the growth performance of finishing beef steers and had little effects on carcass characteristics.

Sodium butyrate and monensin supplementation to postweaning heifer diets: Effects on growth performance, nutrient digestibility, and health

The objective of this study was to evaluate growth and performance of postweaning heifers supplemented with monensin (MON), sodium butyrate (SB), or the combination of MON and SB (MSB) compared with heifers not receiving these feed additives. Forty Holstein heifers [mean age 84.2 ± 1.2 d; body weight (BW) 99.8 ± 10.8 kg (mean ± SD)] were housed in a freestall barn, blocked by birth date, and randomly assigned to 1 of 4 treatments in a randomized complete block design. Treatments were (1) 100 g of soybean meal carrier (control; CON); (2) 0.75 g of SB/kg of BW + carrier (SB); (3) 1 mg of MON/kg of BW + carrier (MON); (4) 1 mg of MON/kg of BW + 0.75 g of SB/kg of BW (MSB). Data were analyzed using single degree of freedom contrasts evaluating CON versus additives (ADD), SB versus MON, and SB and MON versus MSB. Treatments were hand-mixed daily. Feed and orts were measured daily and frozen at -20°C. Orts samples were subsampled for dry matter (DM) determination, and total mixed ration samples were taken weekly and composited monthly for DM and nutrient analysis. Initial BW, heart and paunch girths, body length, blood samples, and fecal coccidia counts were measured before the start and weekly during the 12-wk trial. Blood samples were analyzed for glucose, plasma urea nitrogen (PUN), and ketone concentrations. Apparent total-tract nutrient digestibility was determined from d 21 to 27 and from d 63 to 69 using acid detergent insoluble ash as a marker. Daily dry matter intake (DMI) and metabolizable energy intake were increased in ADD compared with CON, and average BW, final BW, and heart girth tended to increase. Whereas MSB tended to be greater than SB and MON for heart girth, feed efficiency was greater with MON compared with SB. Compared with CON, ADD decreased coccidia counts. No effect of treatment on PUN was detected. Monensin and SB tended to have greater plasma glucose than MSB did. Average blood ketone concentrations were greater with ADD versus CON, in SB versus MON, and in MSB versus SB and MON. During the wk-3 digestibility phase, DMI tended to be greater in heifers fed SB versus MON, as well as in heifers fed MSB versus SB and MON. Digestibility of nutrients were similar, except that starch digestibility was increased in heifers fed MSB versus SB and MON. During the wk-9 digestibility phase, DMI and digestibility of nutrients were similar, except NDF, which tended to be greater in CON than in ADD. Overall, ADD resulted in positive growth and reduced coccidia compared with CON.

Essential oil and monensin affect ruminal fermentation and the protozoal population in continuous culture

The interaction of monensin and essential oil was hypothesized to suppress protozoa and methane production while maintaining normal rumen function. The objective of this study was to determine the effects of feeding monensin (MON) and CinnaGar (CIN, a commercial blend of cinnamaldehyde and garlic oil; Provimi North America, Brookville, OH) on ruminal fermentation characteristics. Continuous culture fermentors (n = 4) were maintained in 4 experimental periods in a 4 × 4 Latin square design. Four dietary treatments were arranged in a 2 × 2 factorial: (1) control diet, 37 g/d of dry matter (40 g/d at ∼92.5% dry matter) of a 50:50 forage:concentrate diet containing no additive; (2) MON at 11 g/909 kg of dry matter; (3) CIN at 0.0043% of dry matter; and (4) a combination of MON and CIN at the levels in (2) and (3). Treatment had no effects on protozoal populations, concentration of NH3N, total N flow of effluent, production of total volatile fatty acids, or flows of conjugated linoleic acid and total C18 fatty acids. The MON decreased acetate:propionate ratio and biohydrogenation of both total C18 and 18:1 cis-9 but increased protozoal generation time, concentration of peptide, and flow of 18:1 trans-11. The MON tended to decrease protozoal counts in effluent and flow of 18:0 but tended to increase propionate production. The CIN decreased true organic matter digestibility and protozoal N flow of effluent but increased nonammonia, nonmicrobial N flow. The CIN tended to decrease protozoal counts, microbial N flow, and neutral detergent fiber digestibility but tended to increase biohydrogenation of total C18, 18:2, and 18:3. The CIN tended to increase isovalerate production. The MON and CIN tended to interact for increased methane production and bacterial N flow. A second experiment was conducted to determine the effects of MON and CIN on protozoal nitrogen and cell volume in vitro. Four treatments included (1) control (feed only), (2) feed + 0.0043% dry matter CIN, (3) feed + 2.82 μM MON, and (4) feed + CIN + MON at the same levels as in (2) and (3). With no interactions, MON addition decreased percentage of protozoa that were motile and tended to decrease cell volume at 6 h. The CIN did not affect cell count or other indicators of motility or volume at either 3 or 6 h. Under the conditions of our study, we did not detect an additive response for MON and CIN to decrease protozoal counts or methane production. A 3-dimensional method is suggested to better estimate protozoal cell volume.

Effects of feeding generic ractopamine (Actogain) with or without the combination of monensin and tylosin phosphate on growth performance, carcass characteristics, and tenderness of finishing steers

ABSTRACT The objectives of this study were to evaluate the effects of generic ractopamine (Actogain) with or without the combination of monensin and tylosin on growth performance, carcass characteristics, and tenderness of finishing steers. A total of 1,230 steers were finished in 6 blocks based on allocated BW, where each block had 3 pens (1 pen per treatment). Steers were assigned to 1 of 3 different finishing diets for the final 33 or 34 d before slaughter: (1) no generic ractopamine with monensin and tylosin (CON + M/T), (2) generic ractopamine without monensin and tylosin (ACTO − M/T), or (3) generic ractopamine with monensin and tylosin (ACTO + M/T). Steers fed ACTO − M/T and ACTO + M/T had greater ( P P P P = 0.01) in steers fed ACTO + M/T compared with steers fed CON + M/T. There were no treatment differences ( P ≥ 0.06) for YG parameters (excluding HCW), calculated YG, or Warner-Bratzler shear force. In conclusion, final BW, ADG, G:F, and HCW were increased in steers fed ACTO with or without M/T compared with steers fed CON + M/T, and other carcass characteristics were minimally affected among treatments.

In vivo ruminal degradation characteristics and apparent digestibility of low-quality prairie hay for steers consuming monensin and Optimase.

Seven ruminally cannulated crossbred steers (BW = 720 ± 62 kg) were used in a randomized crossover design (4 periods, each 18 d) to evaluate in vivo rumen characteristics and apparent digestibility of steers consuming low-quality prairie hay and 1 of 4 isonitrogenous protein supplements. Treatments included 1) 40% CP (DM basis) cottonseed meal and wheat middlings-based supplement (Control), 2) a cottonseed meal and wheat middlings-based supplement with slow-release urea and a fibrolytic feed enzyme (Optimase; Alltech, Inc., Nicholasville, KY) designed to replace 30% of plant-based CP provided in the Control (OPT), 3) the Control plus 0.40 mg∙kg BW∙d monensin (Rumensin 90; Elanco Animal Health, Greenfield, IN; MON), and 4) the OPT plus 0.40 mg∙kg BW∙d monensin (COMBO). Steers were allowed ad libitum access to prairie hay (5.0% CP and 76% NDF) and were provided each respective supplement at 0800 h daily at a rate of 1.0 g/kg of BW. Steers were adapted to diets for 10 d before sample collection. Beginning on d 11, DMI was measured and samples were collected to determine apparent digestibility. On d 15 of the 18-d period, rumen fluid was collected 10 times over a 24-h period. Forage DMI was greater ( ≤ 0.02) for steers consuming the OPT compared with steers consuming the MON or COMBO, although forage DMI was not different ( = 0.10) among steers consuming the Control compared with steers consuming the OPT, MON, or COMBO. Steers fed the MON and COMBO had lower ( ≤ 0.05) passage rate compared with steers fed the Control and the OPT. The MON-fed steers had lower ( = 0.01) ruminal pH and increased ( = 0.03) propionate as a percentage of total VFA production. A time × treatment ( = 0.01) interaction was observed for ruminal NH-N due to a rapid (0 to 1 h after feeding) increase followed by a quick (1 to 4 h after feeding) decline in NH-N by steers consuming the OPT and COMBO that was not observed for steers consuming all other treatments. Apparent digestibility of DM ( = 0.01) and NDF ( = 0.03) were improved for steers fed the COMBO supplement compared with steers consuming all other experimental supplements. This work suggests that the OPT may be an effective replacement for a portion of supplemental degradable intake protein in low-quality forage. Further research is necessary to determine if the combination of monensin and the Optimase consistently improves low-quality forage utilization.

Interaction of sunflower oil with monensin on milk composition, milk fatty acid profile, digestion, and ruminal fermentation in dairy cows

Abstract Four ruminally fistulated multiparous Holstein cows were assigned to a 4 × 4 Latin square design to determine the interaction between sunflower oil and monensin supplementation on intake, milk production, total apparent digestibility of the diet (TTAD), ruminal fermentation characteristics and milk fatty acid profile. The experimental diets consisted of a 2 × 2 factorial arrangement of treatments: (1) control (no sunflower oil and no monensin; CON), (2) diet containing (dry matter (DM) basis) 42 g/kg sunflower oil (OIL), (3) control with monensin (16 mg/kg of DM; MON), and (4) diet containing (DM basis) 42 g/kg sunflower oil and 16 mg/kg monensin (MIX). Sunflower oil supplementation tended to decrease DM intake and yield of milk fat. Sunflower oil decreased milk urea N concentration likely as a result of better N utilization, suggesting that sunflower oil contributes to decrease deamination and amount of amino acids used for gluconeogenesis. There were interactions between monensin and oil supplementation for acetate, propionate, and the acetate to propionate ratio in the rumen as a result of lower proportion of acetate and higher proportion of propionate for cows fed MON compared to those fed CON. Molar proportions of butyrate and isobutyrate in the rumen were decreased by monensin and proportion of butyrate was increased by oil. There was no interaction between monensin and oil for milk FA profile. Sunflower oil supplementation decreased proportions of all short-chain and most medium-chain FA. Compared with cows fed no oil, those supplemented with sunflower oil had higher proportions of total trans FA and monensin had no effect. This study suggests that supplementing dairy cow diets containing a corn-based concentrate with a combination of monensin and sunflower oil adds no further improvements in milk FA profile to supplementing sunflower oil alone although the lack of effect on parameters with large numerical changes such as DM intake and milk yield should be confirmed using more animals.

Investigation of ruminal bacterial diversity in dairy cattle fed supplementary monensin alone and in combination with fat, using pyrosequencing analysis

The objective of this study was to examine and compare the effects of monensin, both alone and together with dietary fat, on ruminal bacterial communities in dairy cattle fed the following 3 diets: a control diet, the control diet supplemented with monensin, and the control diet supplemented with both monensin and fat. Bacterial communities in the liquid and the adherent fractions of rumen content were analyzed using 454 pyrosequencing analysis of 16S rRNA gene amplicons. Most sequences were assigned to phyla Firmicutes and Bacteroidetes, irrespective of diets and fractions. Prevotella was the most dominant genus, but most sequences could not be classified at the genus level. The proportion of Gram-positive Firmicutes was reduced by 4.5% in response to monensin but increased by 12.8% by combination of monensin and fat, compared with the control diet. Some of the operational taxonomic units in Firmicutes and Bacteroidetes were also affected by monensin or by the combination of monensin with fat. The proportion of numerous bacteria potentially involved in lipolysis and (or) biohydrogenation was increased by both monensin and fat. The Shannon diversity index was decreased in the control diet supplemented with both monensin and fat, compared with the other 2 diet groups. Supplementary fats hinder bacterial attachment to plant particles and then result in decreased bacterial diversity in the rumen. The finding of this study may help in understanding the effect of monensin and fat on ruminant nutrition and the adverse effect of monensin and fat, such as milk fat depression and decreased feed digestibility.

Ruminal fermentation characteristics and fatty acid profile of ruminal fluid and milk of dairy cows fed flaxseed hulls supplemented with monensin

Flaxseed hull, a co-product obtained from flax processing, is a rich source of n-3 fatty acids (FA) but there is little information on its value for dairy production. Monensin supplementation is known to modify biohydrogenation of FA by rumen microbes. Therefore, the main objective of the experiment was to determine the effect of feeding a combination of monensin and flaxseed hulls on ruminal fermentation characteristics and FA profile of ruminal fluid and milk. Four ruminally fistulated multiparous Holstein cows averaging 665 ± 21 kg body weight and 190 ± 5 d in milk were assigned to a 4×4 Latin square design (28-d experimental periods) with a 2×2 factorial arrangement of treatments. Treatments were: 1) control, neither flaxseed hulls nor monensin; 2) diet containing (dry matter basis) 19·8% flaxseed hulls; 3) diet with monensin (16 mg/kg dry matter); 4) diet containing 19·8% (dry matter basis) flaxseed hulls and 16 mg monensin/kg. Flaxseed hull supplementation decreased the acetate to propionate ratio in ruminal fluid and monensin had no effect. Concentrations of trans-18:1 isomers (trans9,trans11,trans13/14+6/8) and cis9,12,15-18:3 in ruminal fluid and milk fat were higher and those of cis9,12-18:2 in milk fat tended (P=0·07) to be higher for cows supplemented with flaxseed hulls than for cows fed no flaxseed hulls. Monensin had little effect on milk fatty acid profile. A combination of flaxseed hulls and monensin did not result in better milk fatty acid profile than when feeding only flaxseed hulls.

Effect of monensin, fish oil or their combination on in vitro fermentation and conjugated linoleic acid (CLA) production by ruminal bacteria

An in vitro study was conducted to examine the effect of adding monensin, fish oil, or their combination on rumen fermentation and conjugated linoleic acid (CLA) production by mixed ruminal bacteria when incubated with safflower oil. Concentrate (1 g/100 ml) with safflower oil (0.2 g/100 ml) was added to a mixed solution (600 ml) of strained rumen fluid and buffer (control). Monensin (10 ppm), fish oil (0.02 g/100 ml), or monensin plus fish oil was also added into control mixture. All the culture solutions prepared were incubated anaerobically at 39 °C for 12 h. A higher pH and ammonia concentration were observed from the culture solution containing monensin at 12 h of incubation than those from the control or the culture containing fish oil. Monensin increased ( P < 0.007) the C 3 content over all the collection times of culture solution while reducing the C 4 content at 6 h ( P < 0.018) and 12 h ( P < 0.001) of incubations. Supplementation of monensin, fish oil or their combination changed the content of C 18-fatty acids of ruminal culture. Monensin alone reduced ( P < 0.021) the content of cis-9, trans-11 CLA compared to fish oil at all sampling times, but increased ( P < 0.041) the trans-10, cis-12 CLA production compared to fish oil addition and the control which were similar at incubation for 12 h. The combination of monensin and fish oil increased the content of cis-9, trans-11 CLA ( P < 0.023) and transvaccenic acid (TVA, P < 0.018) significantly compared to the control or monensin alone at incubation for 12 h.

Interaction of monensin and sulfadimethoxine in broilers, as mediated by hepatic microsomal cytochrome P-450 monooxygenases.

The influence of monensin + sulfadimethoxine on cytochrome P-450 monooxygenase activity in broilers, and the possible consequences of modification of this system, including changes in blood levels of sulfadimethoxine, influence on the duration of xylazine-ketamine anesthesia, total antioxidant status and superoxide dismutase activity were studied. The results indicate that the combination of monensin + sulfadimethoxine gave a short-term inhibition of microsomal cytochrome P-450 monooxygenase activity but apparently did not influence the metabolism of other (exogenic) substances (ketamine, xylazine), and did not change the state of antioxidant systems or the relative liver weight. There was a rise in blood sulfadimethoxine levels.

Feeding Monensin to yearling cattle on summer grass

Two grazing trials conducted at different locations in Kansas evaluated feeding Monensin to grazing yearling cattle. In one trial Monensin was fed with and without implant treatments of diethylstilbestrol or Ralgro. Monensin increased weight gain on summer grass; diethylstilbestrol and Ralgro implants also increased weight gains, and the combination of Monensin with either implant was more effective than Monensin alone.