Due to the limited availability of slip systems, deformation twins have to play an additional role to accommodate strain in developing plastic deformation and undoubtedly influence the mechanical properties of materials with hexagonal structure. Therefore, understanding the interfacial characteristic of deformation twin and interaction behavior between different twin variants during plastic deformation is crucial to microstructural design, processing and application of hexagonal close-packed (hcp) materials. In the present study, {112¯2} twinning behavior in deformed titanium, with an emphasis on the misorientation across {112¯2} twinning boundary and the interaction between two {112¯2} twin variants sharing the same [101¯0] zone axis, is investigated by a combination of electron back-scatter diffraction (EBSD) and transmission electron microscopy (TEM) techniques. The results reveal that the actual value of misorientation across {112¯2} twinning boundary is not always equal to the theoretical one. Also, a distinctive phenomenon of one twin penetrating into the interior of the other twin, which is not observed when two {101¯2} twins encounter to each other, is discovered when the interaction between two {112¯2} twins takes place. Accordingly, the possible mechanisms behind such phenomena are proposed and discussed. These findings are expected to provide an insight into understanding the twinning behavior in hcp metals.