Combination Of Bio Research Articles

The Lower - Middle Miocene transition (Karpatian – Badenian) in the Krems Embayment (Central Paratethys, Lower Austria): a multistrati-graphic approach and the role of the Diendorf-Boskovice Fault System.

Abstract The Krems Embayment contains the westernmost fully marine depositional environments of the Karpatian and Bade-nian transgressions in the Central Paratethys. Four drill cores were investigated to analyse the bio- and lithostratigraphic, and tectonic relations. The investigated core sections cover the Karpatian Laa Formation (bio-zones M4, NN4) and the Badenian Gaindorf Formation (M5b-M6, NN4-NN5). Important biostratigraphic indicators identified are Praeorbulina glomerosa glomerosa, Praeorbulina glomerosa circularis and Orbulina suturalis for the Gaindorf Formation. The Laa Formation is indicated by the absence of Praeorbulina, Orbulina and Globigerina falconensis, low numbers of Globorotalia bykovae, and a prominent peak in Helicosphaera ampliaperta abundance at the end of the Karpatian. Cibicidoides lopjanicus and Cassigerinella spp. occur with high percentages in Badenian samples and show much longer stratigraphic ranges than known from literature data. The depositional gap at the Karpatian-Badenian boundary has a minimum duration of 0.41 My in the Krems Embayment. The combination of bio- and lithostratigraphic data allows the correlation across major faults. The Diendorf-Boskovice Fault System played an important role during basin formation and was identified as very active during the early to middle Badenian Stage. The results of this study show the complex interaction of sedimentation, tectonic activity and paleobiological developments in this peripheral part of a marginal sea.

Influence of different growing media on the growth and development of strawberry plants

The optimal production of strawberries requires the essential nutrients and favourable media for vegetative and reproductive growth. The present study sought to determine the effectiveness of growth parameters and fruit yield of strawberries in different media growing under a greenhouse. To analyze the significant effect for the growth and fruit yield among the growing media, four treatments such as control soil (CS), bio plus compost (T1), the combination of bio plus compost, and synthetic nutrient applied media/integrated media (T2) and synthetic nutrient applied soil media (T3) were assayed. Morphology parameters like plant height, canopy area, fresh weight, dry weight of roots were measured in each stage after eight weeks and sixteen weeks and yield attributing parameter as the number of fruits set per plant and number of fruits per plant were measured at the beginning and end of the reproductive stage eight and sixteen weeks respectively. The effects of growing media for the strawberry plant growth and productivity were analyzed using completely randomized block designs through analyzing the variance with a significance level of p < 0.05. The canopy area of the strawberry plants was calculated using the image processing technique applied in HSV colour space. Correspondingly, the vegetative stage and reproductive stage of T2 plants attained the maximum plant height of 16.93 ± 0.31 cm and 19.34 ± 0.21 cm, canopy area with 23.02 ± 1.94 cm2 and 28.78 ± 0.93 cm2, fresh weight of 18.00 ± 3.06 g, and 20.15 ± 3.49 g, dry weight of 5.15 ± 1.26 g and 6.66 ± 2.34 g and the number of fruits set per plant 18.83 ± 2.64 and number of fruits per plant 24.17 ± 2.14 followed by T1, T3, and CS respectively. A comparison of the relative growth and fruit yield at the vegetative and reproductive phases of plants T2 implied better performance. This study demonstrated that bio plus compost with synthetic nutrients act as a better source for the growth and production of strawberries under the greenhouse.

Optimization of a Catalytic Chemoenzymatic Tandem Reaction for the Synthesis of Natural Stilbenes in Continuous Flow

In view of the development of efficient processes for the synthesis of high-value compounds, the combination of bio- and chemocatalysis is highly promising. In addition, implementation of immobilized catalysts into continuous setups allows a straightforward separation of the target compound from the reaction mixture and ensures uniform product quality. In this work, we report the optimization of a chemoenzymatic tandem reaction in continuous flow and its extended application for the synthesis of pharmacologically active resveratrol and pterostilbene. The tandem reaction involves enzymatic decarboxylation of coumaric acid employing encapsulated phenolic acid decarboxylase from B. subtilis and a Heck coupling of the obtained vinylphenol with an aryl iodide using heterogeneous Pd-Ce-Sn oxides implemented in a packed bed reactor. By optimization of the reaction conditions for the limiting cross-coupling step, the yield of (E)-4-hydroxystilbene using the fully continuous setup could be more than doubled compared to previous work. Furthermore, the improved chemoenzymatic cascade could also be applied to the synthesis of resveratrol and pterostilbene in a continuous fashion. Leaching of the metal catalyst at high temperatures limited the process in many perspectives. Therefore, the feasibility of a reactor setup with reversed flow was experimentally evaluated and approved.

Additives the panacea of coal water slurry

Indian high ash coal was used for the preparation of coal water slurry consisting of coarse and fine particles in the proper proportion. Four bimodal samples were prepared with the combination of four coarse fractions such as (212 µm–300 µm, 90 µm–300 µm, 150 µm–212 µm, 75 µm–150 µm) with fine fraction of (<38 µm) in different proportion and labeled as S1, S2, S3 and S4 respectively. Additives are the guiding factor for the flow of the slurry in pipeline. These samples were tried in the presence of different additives like bio, chemical and the combination of chemical and bio additives. In this study bio additives like Sapindous Mukorossi, Saponin from Shikakai, chemical additive like different alcohol groups, and combination of bio and chemical additive like saponin from Shikakai mixed with DDAB (Di dodecyl ammonium bromide), SDBS (Sodium do decyl benzene sulphate) were used. A comparative study revealed saponin from Shikaki when mixed with chemical additive DDAB in the proportion of 0.95:0.05 by weight gives a better result of 70% of coal loading.

Late-Stage Diversification of Tryptophan-Derived Biomolecules.

Pd‐mediated reactions have emerged as a powerful tool for the site‐selective and bioorthogonal late‐stage diversification of amino acids, peptides and related compounds. Indole moieties of tryptophan derivatives are susceptible to C2H‐activation, whereas halogenated aromatic amino acids such as halophenylalanines or halotryptophans provide a broad spectrum of different functionalisations. The compatibility of transition‐metal‐catalysed cross‐couplings with functional groups in peptides, other biologically active compounds and even proteins has been demonstrated. This Review primarily compiles the application of different cross‐coupling reactions to modify halotryptophans, halotryptophan containing peptides or halogenated, biologically active compounds derived from tryptophan. Modern approaches use regio‐ and stereoselective biocatalytic strategies to generate halotryptophans and derivatives on a preparative scale. The combination of bio‐ and chemocatalysis in cascade reactions is given by the biocompatibility and bioorthogonality of Pd‐mediated reactions.

The composition difference of macrophyte litter-derived dissolved organic matter by photodegradation and biodegradation: Role of reactive oxygen species on refractory component.

The overgrowth of macrophytes has become serious due to increasing eutrophication in shallow lakes. The primary degradation processes of macrophytes litter, including photodegradation and biodegradation, induce considerable patchiness in the concentration and bioavailability of dissolved organic matter (DOM). In this study we investigated the composition evolution of DOM derived from emergent aquatic plant litter, Phragmites australis, in microbial degradation, photodegradation, and the combination of bio- and photo-degradation. Results revealed that the effects of photo- and biodegradation on the composition difference of macrophyte litter-derived DOM during short- and long-term degradation phase were different. Although large changes in DOM were observed after five days of incubation, the abundance and chemical composition were similar in the three treatments. However, more concentration of DOM was produced by combined photo- and biodegradation at the long-term degradation phase, and the composition of DOM showed less lignin-like formulae, as well as less condensed aromatic and aromatic compounds when compared to sole treatments. More reactive oxygen species (ROS) were found under the combined treatments, thus the contents of refractory components (condensed aromatic- and aromatic compound groups) were reduced. This study provide deeper insight into the fate of DOM and relevant biogeochemical processes in eutrophic lakes.

Discoloration of denim garment with color free effluent using montmorillonite based nano clay and enzymes: nano bio-treatment on denim garment

In this research, discoloration of indigo dyed denim garment along with color free effluent is introduced by using a combination of bio and nano-treatment. Indigo dyed denim garment were treated with amylase, cellulase, laccase and their combination with modified Montmorillonite based nano clay and mineral clay in the conventional washing machine. Adsorption of released indigo dye in the washing machine on the clay particles essentially open up new opportunity for possible reuse of washing effluent. The colorimetric properties of the treated garments were measured and the color changes expressed in CIE L*a*b* system. The surfaces of the treated fabrics were studied by scanning electron microscope and energy dispersive X-ray analysis. UV-Vis, Raman and transmission electron microscopy were used to study the possible interaction between the Montmorillonite and indigo dye. The results revealed the possibility of using combination of laccase and Montmorillonite in denim washing as a competitor process with conventional bio-washing with enzyme producing lower indigo staining on the denim garment.

Combination of bio- and chemocatalysis for dynamic kinetic resolution: The assembly strategies for nanozeolite-modified flow microchannel reactors

The combination of bio- and chemocatalysts in one-pot is regarded as a breakthrough both for development of new catalytic concepts and for preparation of high-value chemicals. Zeolite nanocrystals (nanozeolites) have been proved as the most used chemical catalysts and as promising supports for enzyme immobilization. These capabilities of nanozeolites endow them with new possibilities for constructing desired platforms for the combination of bio- and chemocatalysis. In this article, several strategies are proposed to develop a one-pot flow microchannel reactor (MCR) by combining enzyme and chemocatalysis via a nanozeolite assembly approach, and the compatibilities of different types of active centers in various assembly strategies are systematically discussed on the basis of the catalytic results of dynamic kinetic resolution (DKR) of sec-alcohol. By precisely controlling the spatial distribution and the ratio of acidic and enzymatic active sites, as well as the reaction conditions, nanozeolite-modified MCR can achieve fast and highly selective one-pot DKR of sec-alcohol within 30min.

InfoBiology by printed arrays of microorganism colonies for timed and on-demand release of messages

This paper presents a proof-of-principle method, called InfoBiology, to write and encode data using arrays of genetically engineered strains of Escherichia coli with fluorescent proteins (FPs) as phenotypic markers. In InfoBiology, we encode, send, and release information using living organisms as carriers of data. Genetically engineered systems offer exquisite control of both genotype and phenotype. Living systems also offer the possibility for timed release of information as phenotypic features can take hours or days to develop. We use growth media and chemically induced gene expression as cipher keys or "biociphers" to develop encoded messages. The messages, called Steganography by Printed Arrays of Microbes (SPAM), consist of a matrix of spots generated by seven strains of E. coli, with each strain expressing a different FP. The coding scheme for these arrays relies on strings of paired, septenary digits, where each pair represents an alphanumeric character. In addition, the photophysical properties of the FPs offer another method for ciphering messages. Unique combinations of excited and emitted wavelengths generate distinct fluorescent patterns from the Steganography by Printed Arrays of Microbes (SPAM). This paper shows a new form of steganography based on information from engineered living systems. The combination of bio- and "photociphers" along with controlled timed-release exemplify the capabilities of InfoBiology, which could enable biometrics, communication through compromised channels, easy-to-read barcoding of biological products, or provide a deterrent to counterfeiting.

Combined approach to demonstrate acetylcholinesterase activity changes in the rat brain following tabun intoxication and its treatment

Reactivation effects of K203 and currently available oximes (obidoxime, HI-6) in combination with atropine on acetylcholinesterase activities in the brain parts of rats poisoned with tabun were studied. The activity was determined by quantitative histochemical and biochemical methods correlating between them very well. The tabun-induced changes in acetylcholinsterase activity as well as in reactivation potency of reactivators used were different in various parts of the brain. Pontomedullar area seems to be important for observed changes following tabun intoxication and its treatment. From the oximes studied, the reactivation effect of K203 was comparable with obidoxime; HI-6 was ineffective. Combination of bio- and histochemical methods allow fine differentiation among the action of different oximes following tabun poisoning.

Biotyping, phage typing, and O-serotyping of clinical isolates of Enterobacter cloacae.

The purpose of this study was to make an independent evaluation of the methods of bio-, phage-, and O-serotyping which had been used only in the laboratory of origin, and to assess the extent of possible cross-infection of Enterobacter cloacae in a Danish university hospital. The material consisted of 237 clinical isolates of E. cloacae from the clinical microbiology laboratory at Hvidovre Hospital. The typability of bio-, phage-, and serotyping was 100%, 83%, and 85%, respectively. Reproducibility of serotyping was 90% and of phage typing 96% if two major differences were allowed to differentiate between patterns. O-serotyping had the highest discriminatory power and combination of all typing methods further increased discrimination. Outbreaks of E. cloacae were not evident in clinical departments, but cross-infections from one department to another could not be completely ruled out. We concluded that the combination of bio-, phage- and O-serotyping is sufficiently discriminating and will be satisfactory in the majority of clinical situations.