Comb Plate Research Articles

Gap Junctions in Ctenophora.

Gap junction proteins form specialized intercellular communication channels, including electrical synapses, that regulate cellular metabolism and signaling. We present a molecular inventory of the gap junction proteins-innexins (INX-like) in ctenophores, focusing on two reference species, Pleurobrachia bachei and Mnemiopsis leidyi. Innexins were identified in more than 15 ctenophore species, including such genera as Euplokamis, Pukia, Hormiphora, Bolinopsis, Cestum, Ocyropsis, Dryodora, Beroe, benthic ctenophores, Coeloplana and Vallicula, and undescribed species of Mertensiidae. The observed diversity of innexins resulted from the independent expansion of this family from the common ancestor of ctenophores. Innexins show the conserved topology with four transmembrane domains connected by two extracellular loops, which bridge intracellular gaps. However, INX-like genes have highly diverse exon organization and low percentage identity for their amino acid sequences within the same species and between ctenophore species. Such a broad scope of molecular diversity differs from innexins in other phyla. We predicted posttranslational modifications in innexins: 249 and 188 for M. leidyi and P. bachei, respectively. Neither their number nor their locations were conserved within or between species. When the number of posttranslational modifications is factored into the innexins' radiation, the potential for molecular and physiological diversity within gap junctions of ctenophores is almost unfathomable. RNA-seq and in situ hybridization data revealed that innexins are expressed across embryogenesis, including early cleavage stages and gastrulation. They are abundant in all adult tissues, with the highest expression level in the aboral organ (the major integrative center and the gravity sensor in ctenophores), followed by tentacles and comb plates. Nevertheless, each organ and tissue has a unique combination of innexins, suggesting their involvement in complex integrative functions and behaviors of ctenophores.

The invention relates to a new type of curved hook gauge for measuring elevator related dimensions

In order to realize a measuring tool that can be easily used in elevator inspection and testing, this paper integrates several types of existing plug gauge (gap gauge and vernier gauge), steel ruler and some tape measure functions, and carries out technical transformation of existing tools through parameter calculation and mechanical optimization design to achieve a tool. It not only solves the direct measurement of the data related to the comb and comb plate of the escalator, but also can be used in the escalator and elevator inspection and detection of other items.

Effect of monochromatic light on the behavior of the ctenophore Mnemiopsis leidyi (A. Agassiz, 1865)

Ctenophores are invertebrate, gelatinous predators that perform complex movements due to their numerous ciliary comb plates. We investigated the behavioral responses of the ctenophore Mnemiopsis leidyi A. Agassiz, 1865 to red, green, and blue lights of different powers and fluxes emitted by LEDs or lasers. White LEDs were used to mimic natural sunlight. When laser light was directed to the aboral organ, the animals tended to leave the illumination zone. The blue-light reaction was six times faster than the red-light reaction. The behavioral strategy of the animals changed significantly when their freedom of maneuvering was restricted. Typical locomotions were ranked according to the laser beam avoidance time from the beginning of exposure to going into darkness. The minimum reaction time was required for turning and moving the ctenophore, while moving along the laser beam and turning around required more time. Typical patterns of behavior of M. leidyi in the light flux were established using cluster analysis. Three preferential behavioral strategies were identified for avoiding laser irradiation: 1) body rotation; 2) shifting sideways; and 3) movement with deviation from the beam. The elementary ability of ctenophores to make decisions in situative conditions has been demonstrated.

Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia

Comb plates are large ciliary structures uniquely seen in comb jellies (ctenophores).1,2,3 A comb plate is constructed from tens of thousands of cilia that are bundled together by structures called compartmenting lamellae (CLs).4,5,6 We previously reported the first component of the CL, CTENO64, and found that it was specifically found in ctenophores and was essential for the determination of ciliary orientation.3 However, CTENO64 is localized only in the proximal region of the CL; therefore, the molecular architecture of the CL over the entire length of a comb plate has not been elucidated. Here, we identified a second CL component, CTENO189. This ctenophore-specific protein was present in the distal region of comb plates, with a localization clearly segregated from CTENO64. Knockdown of the CTENO189 gene using morpholino antisense oligonucleotides resulted in complete loss of CLs in the distal region of comb plates but did not affect the formation of comb plates or the orientation of each cilium. However, the hexagonal distribution of cilia was disarranged, and the metachronal coordination of comb plates along a comb row was lost in the CTENO189 morphants. The morphant comb plate showed asymmetric ciliary-type movement in normal seawater, and in a high-viscosity solution, it could not maintain the normal waveforms but showed a symmetric flagellar-type movement. Our findings demonstrated two distinct compartments of a comb plate: the proximal CL as the building foundation that rigidly fixes the ciliary orientation, and the distal CL that reinforces the elastic connection among cilia to overcome the hydrodynamic drag of giant multiciliary plates.

Research on a new designed upward turning comb of escalator

Abstract Escalator is an important transportation method for people in their daily life. The comb fixed at the entrance and exit is an important component of the escalator. Its function is to facilitate the transportation of passengers and prevent the escalator from being damaged by foreign matters to a certain extent. In order to avoid harm to passengers caused by the comb, the end of the comb should be made of easily broken material traditionally such as plastic and aluminum to ensure that the object can be released when clamped. This greatly increases the loss of comb and the repair time of maintenance personnel and leaves potential safety hazards. The article researches a new designed upward turning comb of escalator. The device comprises comb plate, mounting base, guide rod, spring, comb teeth, rotating shaft, upper baffle, chute, notch, mounting hole, long hole, guide hole, lower baffle, positioning block and arc push block. Compared with the prior technology, this new comb has the advantages of simple structure, convenient operation, repeated use, difficult to damage.

Neuropeptide repertoire and 3D anatomy of the ctenophore nervous system

Ctenophores are gelatinous marine animals famous for locomotion by ciliary combs. Due to the uncertainties of the phylogenetic placement of ctenophores and the absence of some key bilaterian neuronal genes, it has been hypothesized that their neurons evolved independently. Additionally, recent whole-body, single-cell RNA sequencing (scRNA-seq) analysis failed to identify ctenophore neurons using any of the known neuronal molecular markers. To reveal the molecular machinery of ctenophore neurons, we have characterized the neuropeptide repertoire of the ctenophore Mnemiopsis leidyi. Using the machine learning NeuroPID tool, we predicted 129 new putative neuropeptide precursors. Sixteen of them were localized to the subepithelial nerve net (SNN), sensory aboral organ (AO), and epithelial sensory cells (ESCs), providing evidence that they are neuropeptide precursors. Four of these putative neuropeptides had a behavioral effect and increased the animals' swimming speed. Intriguingly, these putative neuropeptides finally allowed us to identify neuronal cell types in single-cell transcriptomic data and reveal the molecular identity of ctenophore neurons. High-resolution electron microscopy and 3D reconstructions of the nerve net underlying the comb plates confirmed a more than 100-year-old hypothesis of anastomoses between neurites of the same cell in ctenophores and revealed that they occur through a continuous membrane. Our work demonstrates the unique ultrastructure of the peptidergic nerve net and a rich neuropeptide repertoire of ctenophores, supporting the hypothesis that the first nervous system(s) evolved as nets of peptidergic cells.

Improving Bridge Expansion and Contraction Installation Replacement Decision System Using Hybrid Chaotic Whale Optimization Algorithm

Bridge expansion and contraction installation (BECI) has proved to be an essential component of the bridge structure due to its stability, comfort, and durability benefits. At present, traditional replacement technologies for modular type, comb plate type, and seamless type BECIs are widely applied worldwide. Nevertheless, it is unfortunate that the research conducted on decision-making (DM) approaches for the technical condition assessment and the optimal replacement plan selection of existing BECIs remain scarce, which results in the waste of resources and the increase in cost. Therefore, a BECI technical condition assessment approach, which contains specific on-site inspection regulations with both qualitative and quantitative descriptions, is proposed in this research, and a corresponding calculation program has been developed based on the MATLAB platform, which provides the basis for the necessity of replacement. Simultaneously, the hybrid chaotic whale optimization algorithm is designed and performed to improve and automate the process of optimal replacement plan selection under the assistance of the analytic hierarchy process (AHP), where both the achievement in consistency modification and the reservation of initial information are perused, and its superiority and effectiveness are verified via the comparative experimental analysis. The improved BECI replacement decision system is established, and the corresponding case study demonstrates that the proposed system in this research proves reasonable and feasible. The improved system can effectively assist bridge managers in making more informed operation and maintenance (O and M) decisions in actual engineering projects.

CTENO64 Is Required for Coordinated Paddling of Ciliary Comb Plate in Ctenophores.

Ctenophores, or comb jellies, are one of the earliest branching basal metazoan groups, whose phylogenetic position continues to be controversial. They have eight rows of iridescent structures, called comb plates, which are huge multiciliated paddle-like structures used for locomotion and uniquely found in this group of animals [1]. Despite a number of morphological and physiological studies over the past 50 years, the molecular nature of comb plates remains completely unknown. Here, we identified a protein CTENO64 that is specifically localized in the comb plates. This protein is only found in ctenophores and not in other animals or eukaryotic species that possess multiciliary cells or tissues. It is localized to regions, called compartmenting lamella (CL), which are uniquely seen in ctenophore multicilia, connecting adjacent cilia in the comb plates. Knockdown of the CTENO64 gene did not affect the formation of comb plates but caused the loss or misformation of CLs and the disruption of ciliary orientation, resulting in aberrant and non-planar waveforms in the mid-distal region of the comb plates. We propose that CLs have been convergently acquired in ctenophores to overcome the hydrodynamic constraints of possessing extremely long multicilia. Our findings provide the initial step in unveiling the molecular structure and evolutionary significance of ciliary comb plates and shed light not only on the hidden biology of ctenophores but also on the unique evolutionary pathway of these animals. VIDEO ABSTRACT.

Animal Evolution: The Hidden Biology of the Ctenophore Cilium.

A new study uncovers the function of a completely novel protein in the eye-catchingly iridescent cilia of the ctenophore comb plate.

CTENO64 is Required for Coordinated Paddling of Ciliary Comb Plate in Ctenophores

Motile cilia are oscillating nanomachinery that are essential for cell locomotion and the generation of extracellular fluid flow. They typically comprise an axoneme with a 9+2 microtubule structure with dynein arms on each microtubule doublet that generate force by sliding between adjacent microtubules. These structures are efficient for motility and have therefore been conserved in cellular-level aquatic microflow throughout eukaryotic evolution. Diversity in the ciliary structures is seen in the basic microtubule arrangements but also in the bundling of multiple cilia, such as compound cilia. Studies on the structural diversity of cilia shed light on the mechanisms underlying ciliary formation and the biological adaptation to different aquatic environments. Ctenophores (comb jellies) have eight rows of iridescent comb plates that facilitate locomotion. A comb plate consists of tens of thousands of cilia to form a “puddle-like” ciliary plate with lengths of up to 1 mm. It exhibits structural color by light diffraction and is reported to have properties similar to photonic crystals. Here we identified a protein specific to the construction of comb plates (CTENO64). This protein is specifically present in ctenophore species and is localized on the compartmenting lamella (CL), which is the structure connecting adjacent cilia on the comb plates. Knockdown of the CTENO64 gene caused loss or misformation of CLs, resulting in aberrant waveforms in the comb plates. Our findings provide the initial characterization of a protein involved in the bundling of cilia into giant compound ciliary comb plates.

Fracture Analysis of Escalator Step and Comb Plate

The escalator step fractured during applications. The causes for the fracture of escalator step and comb plate were investigated by means of macroscopic fractography, optical microscope and scanning electron microscope. The analysis results indicate that some porosity defects with different size distributed in the escalator step support. The fracture mode of escalator step is overload fracture.

Analysis the Cause of Step Fracture in the Escalator Step Arch Event

The Escalator steps are one of the most important parts of escalators. The step is a special structure of the four-wheel car, there are two main wheel and two auxiliary wheel. In this paper, by taking a variety of analytical methods, such as visual inspection, chemical composition analysis, and microstructure, analysis on the cause of step fracture in the escalator step arch event. According to analysis, we can conclude that the escalator step collision event is not caused by the step body. Due to improper adjustment of escalator ladder, causing the step pedal to align with the upper comb plate, eventually triggering a step collision event.

Research on Construction Technology of Fixation Stent Installing Comb Expansion Joint

The hot-rolling-whole-molded and comb-type-designed bridge expansion devices are widely used in the urban and highway bridges, owning the characteristics of large expansion allowance and small burial depth. Whereas using traditional craft to install comb-type expansion joint, such phenomenon like settlement, water seepage, bumping and noise in driving would happen with slow construction. Through continuous improvement for many years, Jinan Urban Construction Group has developed a full construction technology of installing comb expansion joint based on fixation stent by using tailor-made location frame to integrally move the anchor bolt group into the groove for firm weld with embedded steel, matching with moving lifting tool. Through this construction technology, the efficiency of construction is improved, the construction period is shortened, and the smoothness of asphalt after installing comb plate would meet the design requirements, also such quality defects like bumping and abnormal sound would be precluded. hot-rolling-whole-molded and comb-type-designed bridge expansion devices

A cleavage clock regulates features of lineage-specific differentiation in the development of a basal branching metazoan, the ctenophore Mnemiopsis leidyi.

BackgroundAn important question in experimental embryology is to understand how the developmental potential responsible for the generation of distinct cell types is spatially segregated over developmental time. Classical embryological work showed that ctenophores, a group of gelatinous marine invertebrates that arose early in animal evolution, display a highly stereotyped pattern of early development and a precocious specification of blastomere fates. Here we investigate the role of autonomous cell specification and the developmental timing of two distinct ctenophore cell types (motile compound comb-plate-like cilia and light-emitting photocytes) in embryos of the lobate ctenophore, Mnemiopsis leidyi.ResultsIn Mnemiopsis, 9 h after fertilization, comb plate cilia differentiate into derivatives of the E lineage, while the bioluminescent capability begins in derivatives of the M lineage. Arresting cleavage with cytochalasin B at the 1-, 2- or 4-cell stage does not result in blastomere death; however, no visible differentiation of the comb-plate-like cilia or bioluminescence was observed. Cleavage arrest at the 8- or 16-cell stage, in contrast, results in the expression of both differentiation products. Fate-mapping experiments indicate that only the lineages of cells that normally express these markers in an autonomous fashion during normal development express these traits in cleavage-arrested 8- and 16-cell stage embryos. Lineages that form comb plates in a non-autonomous fashion (derivatives of the M lineage) do not. Timed actinomycin D and puromycin treatments show that transcription and translation are required for comb formation and suggest that the segregated material might be necessary for activation of the appropriate genes. Interestingly, even in the absence of cytokinesis, differentiation markers appear to be activated at the correct times. Treatments with a DNA synthesis inhibitor, aphidicolin, show that the number of nuclear divisions, and perhaps the DNA to cytoplasmic ratio, are critical for the appearance of lineage-specific differentiation.ConclusionOur work corroborates previous studies demonstrating that the cleavage program is causally involved in the spatial segregation and/or activation of factors that give rise to distinct cell types in ctenophore development. These factors are segregated independently to the appropriate lineage at the 8- and the 16-cell stages and have features of a clock, such that comb-plate-like cilia and light-emitting photoproteins appear at roughly the same developmental time in cleavage-arrested embryos as they do in untreated embryos. Nuclear division, which possibly affects DNA-cytoplasmic ratios, appears to be important in the timing of differentiation markers. Evidence suggests that the 60-cell stage, just prior to gastrulation, is the time of zygotic gene activation. Such cleavage-clock-regulated phenomena appear to be widespread amongst the Metazoa and these cellular and molecular developmental mechanisms probably evolved early in metazoan evolution.

Cilia and the life of ctenophores

AbstractCtenophores, or comb jellies, are a distinct phylum of marine zooplankton with eight meridional rows of giant locomotory comb plates. Comb plates are the largest ciliary structures known, and provide unique experimental advantages for investigating the biology of cilia. Here, I review published and unpublished work on how ctenophores exploit both motile and sensory functions of cilia for much of their behavior. The long‐standing problem of ciliary coordination has been elucidated by experiments on a variety of ctenophores. The statocyst of ctenophores is an example of how mechanosensory properties of motile cilia orient animals to the direction of gravity. Excitation or inhibition of comb row beating provides adaptive locomotory responses, and global reversal of beat direction causes escape swimming. The diverse types of prey and feeding mechanisms of ctenophores are related to radiation in body form and morphology. The cydippid Pleurobrachia catches copepods on tentacles and undergoes unilateral ciliary reversal to sweep prey into its mouth. Mnemiopsis uses broad muscular lobes and ciliated auricles to capture and ingest prey. Beroë has giant smooth muscles and toothed macrocilia to rapidly engulf or bite through ctenophore prey, and uses reversible tissue adhesion to keep its mouth closed while swimming. Ciliary motor responses are calcium‐dependent, triggered by voltage‐activated calcium channels located along the length (reversed beating) or at the base (activation of beating) of ciliary membranes. Ciliary and muscular responses to stimuli are regulated by epithelial and mesogleal nerve nets with ultrastructurally identifiable synapses onto effector cells. Post‐embryonic patterns of comb row development in larval and adult stages are described and compared with regeneration of comb plates after surgical removal. Truly, cilia and ctenophores, like love and marriage, go together like a horse and carriage.

Patterns of comb row development in young and adult stages of the ctenophores Mnemiopsis leidyi and Pleurobrachia pileus

The development of comb rows in larval and adult Mnemiopsis leidyi and adult Pleurobrachia pileus is compared to regeneration of comb plates in these ctenophores. Late gastrula embryos and recently hatched cydippid larvae of Mnemiopsis have five comb plates in subsagittal rows and six comb plates in subtentacular rows. Subsagittal rows develop a new (sixth) comb plate and both types of rows add plates at similar rates until larvae reach the transition to the lobate form at ∼5 mm size. New plate formation then accelerates in subsagittal rows that later extend on the growing oral lobes to become twice the length of subtentacular rows. Interplate ciliated grooves (ICGs) develop in an aboral-oral direction along comb rows, but ICG formation itself proceeds from oral to aboral between plates. New comb plates in Mnemiopsis larvae are added at both aboral and oral ends of rows. At aboral ends, new plates arise as during regeneration: local widening of a ciliated groove followed by formation of a short split plate that grows longer and wider and joins into a common plate. At oral ends, new plates arise as a single tuft of cilia before an ICG appears. Adult Mnemiopsis continue to make new plates at both ends of rows. The frequency of new aboral plate formation varies in the eight rows of an animal and seems unrelated to body size. In Pleurobrachia that lack ICGs, new comb plates at aboral ends arise between the first and second plates as a single small nonsplit plate, located either on the row midline or off-axis toward the subtentacular plane. As the new (now second) plate grows larger, its distance from the first and third plates increases. Size of the new second plate varies within the eight rows of the same animal, indicating asynchronous formation of plates as in Mnemiopsis. New oral plates arise as in Mnemiopsis. The different modes of comb plate formation in Mnemiopsis versus Pleurobrachia are accounted for by differences in mesogleal firmness and mechanisms of ciliary coordination. In both cases, the body of a growing ctenophore is supplied with additional comb plates centripetally from opposite ends of the comb rows.

Regeneration of ciliary comb plates in the ctenophore Mnemiopsis leidyi. i. morphology

Regeneration of missing body parts in model organisms provides information on the mechanisms underlying the regeneration process. The aim here is to use ctenophores to investigate regeneration of their giant ciliary swimming plates. When part of a row of comb plates on Mnemiopsis is excised, the wound closes and heals, greatly increasing the distance between comb plates near the former cut edges. Video differential interference contrast (DIC) microscopy of the regeneration of new comb plates between widely separated plates shows localized widenings of the interplate ciliated groove (ICG) first, followed by growth of two opposing groups of comb plate cilia on either side. The split parts of a new plate elongate as their bases extend laterally away from the ICG widening and continue ciliogenesis at both ends. The split parts of a new plate grow longer and move closer together into the ICG widening until they merge into a single plate that interrupts the ICG in a normal manner. Video DIC snapshots of dissected gap preparations 1.5-3-day postoperation show that ICG widenings and/or new plates do not all appear at the same time or with uniform spacing within a gap: the lengths and distances between young plates in a gap are quite variable. Video stereo microscopy of intact animals 3-4 days after the operation show that all the new plates that will form in a gap are present, fairly evenly spaced and similar in length, but smaller and closer together than normal. Normal development of comb plates in embryos and growing animals is compared to the pattern of comb plate regeneration in adults. Comb plate regeneration differs in the cydippid Pleurobrachia that lacks ICGs and has a firmer mesoglea than Mnemiopsis. This study provides a morphological foundation for histological, cellular, and molecular analysis of ciliary regeneration in ctenophores.

Eoandromedaand the origin ofCtenophora

The Ediacaran fossil Eoandromeda octobrachiata had a high conical body with eight arms in helicospiral arrangement along the flanks. The arms carried transverse bands proposed to be homologous to ctenophore ctenes (comb plates). Eoandromeda is interpreted as an early stem-group ctenophore, characterized by the synapomorphies ctenes, comb rows, and octoradial symmetry but lacking crown-group synapomorphies such as tentacles, statoliths, polar fields, and biradial symmetry. It probably had a pelagic mode of life. The early appearance in the fossil record of octoradial ctenophores is most consistent with the Planulozoa hypothesis (Ctenophora is the sister group of Cnidaria+ Bilateria) of metazoan phylogeny.

Ctenophores

Ctenophores

A New Comb Circular Polarizer Suitable for Millimeter-Band Application

This letter presents a new polarizer which has a simple comb structure inside a circular waveguide. The electrical performance of the proposed comb polarizer is optimized by a circular waveguide radius and by the physical parameters of the comb plates. This polarizer is suitable for providing good performance in millimeter-band application because of its simple structure and low fabrication cost. In our experiments the dual-band comb polarizer designed in band 1(K) and band 2(Ka) showed good electrical performance without any tuning elements.