We study optical frequency comb (OFC) generation using cascaded injection of semiconductor lasers in this work. The OFC generation system is operated in two cascaded optical injection stages. When a master laser optically injects into the first stage with proper injection power and frequency, period-one (P1) dynamics are invoked in an optically injected semiconductor laser of the first stage. Another semiconductor laser in the second stage is then optically injected by the P1 dynamics. With proper injection power adjusted in the second stage, the P1 dynamics are regenerated, and the semiconductor laser relaxation oscillations (ROs) become undamped so that subharmonic oscillations appear. Because a subharmonic oscillation frequency is half of an oscillation frequency of the P1 dynamics, extra optical frequency components appear in the middle of the adjacent optical frequency components of the P1 dynamics, thus signaling OFC generation. The OFC signals exhibit at least 15 comb lines, resulting in a bandwidth greater than 140 GHz. Microwave comb signals are obtained after photodetection, although the microwave linewidth is on the order of a few megahertz because of the semiconductor laser noise. Thus, we propose a cascaded injection-locking scheme to stabilize the P1 dynamics and OFC signals. We have demonstrated pure microwave generations with a linewidth of less than 3 Hz and low phase noise.